FastQCFastQC Report
Sat 18 Jun 2016
SRR3552605_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552605_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160652
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23910.20600490069374802No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC14280.12303429451721963No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13280.11441844756223227No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC12200.1051133328508459No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGC207.0333155E-445.016
TACGCAT1100.040.90909216
CGAATAT1550.040.6451614
TATACGG1000.040.5000042
TATTGCG950.040.263161
GCTACGA1700.039.70588310
AACGCGT1100.038.86363627
GACCGAT4600.038.6413049
GTCGATG356.2483414E-638.571431
ACGCATT1050.038.5714317
TACGAGT356.2483414E-638.571432
CTACGAA1700.038.3823511
CAATGCG659.094947E-1238.0769271
TCGTTGA1200.037.49999624
ATTCGCG301.1399288E-437.4999961
CGTTCGG301.1399288E-437.4999962
TAACGCG1150.037.17391226
ATCGTAG552.748493E-936.818181
GTATACG552.748493E-936.818181
TATCGCG552.748493E-936.818181
Produced by FastQC (version 0.11.5)