FastQCFastQC Report
Sat 18 Jun 2016
SRR3552604_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552604_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1318556
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28400.21538713562412212No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC17900.13575456787576712No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC17080.12953564353732416No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAG302.1657452E-645.0000041
TAACGCA2200.039.88636426
GCGATAT800.039.3759
TCTAACG2300.039.13043624
TCGCAAT700.038.57142622
TACGGGT1500.037.5000044
ATCTCGA250.002107220736.011
CGAACTA250.002107220736.024
CACGGAT250.002107220736.022
ACGTAGT250.002107220736.023
TGTCGAA250.002107220736.040
CGCACGG1950.034.6153832
GGGCGAT24400.034.303287
ACGTACG606.4337655E-933.7500041
CTCACGA401.5607407E-533.7524
CATGCGG2600.033.752
CGTAAGG2350.033.510642
CGTATGG1750.033.428572
ACGGGTA1550.033.3870965
TAGGGCA6150.033.2926835
Produced by FastQC (version 0.11.5)