##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552604_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1318556
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.800152591167915	31.0	31.0	34.0	30.0	34.0
2	31.92411774698989	33.0	31.0	34.0	30.0	34.0
3	31.943218945573793	33.0	31.0	34.0	30.0	34.0
4	35.60197519104232	37.0	35.0	37.0	33.0	37.0
5	35.50633723558195	37.0	35.0	37.0	33.0	37.0
6	35.44288221357303	37.0	35.0	37.0	33.0	37.0
7	35.78844508689809	37.0	35.0	37.0	35.0	37.0
8	35.65261771210324	37.0	35.0	37.0	35.0	37.0
9	37.44443163581979	39.0	37.0	39.0	35.0	39.0
10	36.94964567299379	39.0	37.0	39.0	33.0	39.0
11	36.80205694714521	39.0	37.0	39.0	32.0	39.0
12	36.63600180803849	39.0	35.0	39.0	32.0	39.0
13	36.49292483595691	39.0	35.0	39.0	32.0	39.0
14	37.54151966241859	40.0	36.0	41.0	32.0	41.0
15	37.68972573026856	40.0	36.0	41.0	33.0	41.0
16	37.75967725299494	40.0	36.0	41.0	33.0	41.0
17	37.69058500359484	40.0	36.0	41.0	33.0	41.0
18	37.70097515767248	39.0	36.0	41.0	33.0	41.0
19	37.65538134140681	39.0	36.0	41.0	33.0	41.0
20	37.57683177657984	39.0	36.0	41.0	33.0	41.0
21	37.42065183427932	39.0	35.0	41.0	32.0	41.0
22	37.4932858369307	39.0	35.0	41.0	33.0	41.0
23	33.910502853121145	39.0	35.0	41.0	11.0	41.0
24	35.37174909522235	39.0	35.0	41.0	18.0	41.0
25	36.30313767485037	39.0	34.0	41.0	30.0	41.0
26	36.81575526560874	39.0	35.0	41.0	31.0	41.0
27	31.261786378432163	38.0	33.0	40.0	0.0	41.0
28	33.53376724234693	38.0	32.0	40.0	16.0	41.0
29	35.26767084598606	38.0	33.0	40.0	25.0	41.0
30	36.16658678129711	38.0	35.0	40.0	30.0	41.0
31	36.5019475850855	38.0	35.0	40.0	30.0	41.0
32	35.127341576694505	38.0	35.0	40.0	27.0	41.0
33	35.713488088484674	38.0	35.0	40.0	27.0	41.0
34	36.008409957559635	38.0	35.0	40.0	29.0	41.0
35	36.20046930126593	38.0	35.0	40.0	30.0	41.0
36	36.239935201842016	38.0	35.0	40.0	30.0	41.0
37	36.22934331192608	38.0	35.0	40.0	30.0	41.0
38	36.181944490791444	38.0	35.0	40.0	30.0	41.0
39	36.150932535288604	38.0	35.0	40.0	30.0	41.0
40	36.03214425477568	38.0	35.0	40.0	30.0	41.0
41	35.9456238491198	38.0	35.0	40.0	30.0	41.0
42	35.880346378917544	38.0	35.0	40.0	30.0	41.0
43	35.831436055806506	38.0	35.0	40.0	30.0	41.0
44	35.74440448490621	38.0	35.0	40.0	29.0	41.0
45	35.68303811138852	38.0	34.0	40.0	29.0	41.0
46	35.58200713507807	37.0	34.0	40.0	29.0	41.0
47	35.497184799128746	37.0	34.0	40.0	29.0	41.0
48	35.45128837910563	37.0	34.0	40.0	29.0	41.0
49	35.40160599929013	37.0	34.0	40.0	29.0	41.0
50	35.28332281677835	37.0	34.0	40.0	28.0	41.0
51	34.531366889233375	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	18.0
9	65.0
10	113.0
11	87.0
12	71.0
13	72.0
14	82.0
15	99.0
16	174.0
17	285.0
18	509.0
19	906.0
20	1617.0
21	2593.0
22	3878.0
23	5480.0
24	7606.0
25	10214.0
26	13336.0
27	16886.0
28	20781.0
29	26433.0
30	33602.0
31	42854.0
32	55132.0
33	75308.0
34	113521.0
35	123212.0
36	123025.0
37	156597.0
38	217421.0
39	266458.0
40	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.84626515673206	18.40376897151126	22.677231759591553	16.072734112165126
2	31.222337162775037	24.177054292726286	25.853054401936664	18.747554142562013
3	32.88294164222073	24.299157563273763	25.706302955657552	17.111597838847953
4	28.923686214313232	26.083230442999767	25.66132951501491	19.33175382767209
5	25.53422076878039	28.861800333091654	24.763529194057742	20.84044970407021
6	25.507524898449518	33.84535810386514	24.60540166667172	16.041715331013624
7	81.04214003804161	6.014458240681473	8.091199767017859	4.852201954259053
8	83.28406226205031	4.439250210078298	7.569341006373639	4.70734652149776
9	77.77978333874329	6.466392022788566	9.852975527774323	5.9008491106938195
10	42.70656688073923	24.095677392541536	16.70789864063415	16.48985708608508
11	33.25524285657947	24.50574719617521	23.154572122837408	19.084437824407914
12	27.891875657916692	23.29245022585313	28.420332545602918	20.395341570627263
13	26.01952438880108	23.46582170192241	29.932289565251686	20.58236434402483
14	22.782725951722945	25.970758921122805	30.601430655960005	20.64508447119425
15	22.46935283749799	24.2510746604619	31.930005248165415	21.349567253874692
16	25.51571567684649	23.98563276796738	29.710228462044842	20.788423093141283
17	25.33316749535097	23.612042264416527	28.009125133858554	23.045665106373942
18	27.38177218108294	22.76247652735265	28.11985232329912	21.735898968265285
19	25.45580164968344	25.670430379900438	26.797724177054295	22.07604379336183
20	26.646270617250995	25.2850087519984	27.075603918225692	20.993116712524916
21	25.969317950849263	26.229981889278882	27.802383819875683	19.998316339996176
22	25.00136512973283	23.48516103980415	28.202973555920263	23.310500274542758
23	21.85489277664354	32.45868965747378	25.41196581715149	20.274451748731188
24	23.988893911218028	24.20594953873783	28.556617997263672	23.248538552780467
25	23.64086166988736	25.654428025810056	26.739023598542648	23.965686705759936
26	22.871838587060388	25.572596082381025	27.600041257254148	23.95552407330443
27	19.610543655332044	37.047345732756135	23.250434566298285	20.091676045613536
28	21.914427601103025	26.570430076538276	28.21086097215439	23.304281350204313
29	23.472799031667975	26.16324221345169	27.807540976644145	22.55641777823619
30	25.491901747062695	24.488758915055563	28.141087674698685	21.878251663183057
31	25.88050867767467	24.816996775260208	27.042537442474952	22.25995710459017
32	25.523451411999186	28.14017758821013	26.001701861733594	20.334669138057087
33	26.101280491689394	24.67138293709179	26.16582079183592	23.0615157793829
34	22.950561068320194	25.82362827214013	29.970816559933745	21.254994099605934
35	24.06761639247783	26.798330901379998	27.845461247000507	21.288591459141667
36	25.90401924529561	28.187577926155583	25.318909473696984	20.58949335485182
37	25.18019712473342	26.92324027193384	27.067261458747293	20.82930114458544
38	24.703084283109707	27.38768774325853	26.576193957632437	21.33303401599932
39	25.60884786084171	24.827159407715712	26.501566865571125	23.062425865871454
40	26.141324297185708	24.46016703120687	27.472780829938202	21.92572784166922
41	23.37056598278723	25.704406942139734	26.43664736272104	24.488379712351996
42	24.60957289641092	25.078798321800516	26.669022779464807	23.642606002323756
43	24.334423414705178	24.086955730359573	28.10384996920874	23.474770885726507
44	23.93557801109699	24.897236067334266	28.258564672262686	22.90862124930606
45	25.018732613556043	24.597665931519025	26.56534876031052	23.81825269461441
46	24.205570336034267	25.09904774617081	27.53405998683408	23.161321930960842
47	22.68830447853561	25.82135305591875	29.039722241603695	22.450620223941947
48	23.645715464492977	25.323156543976893	28.658092640737294	22.373035350792836
49	24.54366746653157	24.57969172337011	28.48267347006877	22.393967340029548
50	22.298787461435086	24.919988229548082	28.98837819554118	23.79284611347565
51	22.42635125091388	24.328962895773863	28.186591999126314	25.05809385418594
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1513.0
1	2255.5
2	2998.0
3	5069.5
4	7141.0
5	5070.0
6	2999.0
7	2844.0
8	2689.0
9	2784.0
10	2879.0
11	3068.0
12	3257.0
13	3330.0
14	3403.0
15	3501.0
16	3599.0
17	3449.5
18	3300.0
19	3602.0
20	3904.0
21	4499.0
22	5094.0
23	5467.0
24	5840.0
25	6446.5
26	8949.5
27	10846.0
28	12244.0
29	13642.0
30	16020.5
31	18399.0
32	21915.5
33	25432.0
34	28519.5
35	31607.0
36	34710.0
37	37813.0
38	40270.5
39	42728.0
40	47433.5
41	52139.0
42	56422.0
43	60705.0
44	67367.5
45	74030.0
46	82616.5
47	91203.0
48	99111.0
49	107019.0
50	109080.5
51	111142.0
52	105570.0
53	99998.0
54	95431.5
55	90865.0
56	85572.0
57	80279.0
58	75496.0
59	70713.0
60	66102.5
61	61492.0
62	58154.0
63	54816.0
64	48009.5
65	41203.0
66	34393.5
67	27584.0
68	24509.0
69	21434.0
70	17849.5
71	14265.0
72	12094.0
73	9923.0
74	7840.0
75	4830.0
76	3903.0
77	2897.0
78	1891.0
79	1531.0
80	1171.0
81	914.0
82	657.0
83	391.0
84	125.0
85	92.0
86	59.0
87	37.0
88	15.0
89	15.5
90	16.0
91	14.5
92	13.0
93	6.5
94	0.0
95	1.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1318556.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.97231396093297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.67677992008956	22.08263579395486
2	10.026286170237265	6.010219939130232
3	3.9304919352541856	3.534178149130604
4	2.099357713951259	2.5169043407541456
5	1.2878148457393466	1.9299395440025076
6	0.8734765328853675	1.5708067728688455
7	0.6835126775140804	1.4340519596710963
8	0.4941851094859442	1.1849497005064602
9	0.43168509649668185	1.1644741120008775
>10	5.733026239671248	41.769586515429964
>50	0.7171594001607693	13.543075416792997
>100	0.04239361058944456	2.100297765243111
>500	0.0025538319499123067	0.4875868916497061
>1k	0.0012769159749561533	0.671293098864666
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2840	0.21538713562412212	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1790	0.13575456787576712	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1708	0.12953564353732416	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.584054071271907E-5	0.0	0.0	0.11269904349910054	0.0
2	7.584054071271907E-5	0.0	0.0	0.516701603875755	0.0
3	7.584054071271907E-5	0.0	0.0	0.7077439259310944	0.0
4	7.584054071271907E-5	0.0	0.0	0.9101623290933415	0.0
5	7.584054071271907E-5	0.0	0.0	1.5284902575241401	0.0
6	7.584054071271907E-5	0.0	0.0	2.0975976750323837	0.0
7	7.584054071271907E-5	0.0	0.0	2.613995916745288	0.0
8	7.584054071271907E-5	0.0	0.0	3.432315351035527	0.0
9	7.584054071271907E-5	0.0	0.0	3.818267862722554	0.0
10	7.584054071271907E-5	0.0	0.0	4.549446515733878	0.0
11	7.584054071271907E-5	0.0	0.0	5.436856682613405	0.0
12	7.584054071271907E-5	0.0	0.0	6.167580292380452	0.0
13	7.584054071271907E-5	0.0	0.0	6.433022184874969	0.0
14	7.584054071271907E-5	0.0	0.0	6.556793947318127	0.0
15	7.584054071271907E-5	0.0	0.0	6.726904280136756	0.0
16	1.5168108142543814E-4	0.0	0.0	7.070006886321097	0.0
17	1.5168108142543814E-4	0.0	0.0	7.4398053628363146	0.0
18	1.5168108142543814E-4	0.0	0.0	7.884458453034987	0.0
19	1.5168108142543814E-4	0.0	0.0	8.151948040128747	0.0
20	1.5168108142543814E-4	0.0	0.0	8.405482967731366	0.0
21	1.5168108142543814E-4	0.0	0.0	8.692008530544019	0.0
22	1.5168108142543814E-4	0.0	0.0	8.995674055557746	0.0
23	1.5168108142543814E-4	0.0	0.0	9.301614796792855	0.0
24	1.5168108142543814E-4	0.0	0.0	9.549006640597744	0.0
25	1.5168108142543814E-4	0.0	0.0	9.780396130312251	0.0
26	1.5168108142543814E-4	0.0	0.0	10.007841911909695	0.0
27	1.5168108142543814E-4	0.0	0.0	10.26183188275659	0.0
28	1.5168108142543814E-4	0.0	0.0	10.517642026580592	0.0
29	1.5168108142543814E-4	0.0	0.0	10.800982286683311	0.0
30	1.5168108142543814E-4	0.0	0.0	11.125579800933748	0.0
31	1.5168108142543814E-4	0.0	0.0	11.40816165562934	0.0
32	1.5168108142543814E-4	0.0	0.0	11.696962434663375	0.0
33	1.5168108142543814E-4	0.0	0.0	11.996153367775051	0.0
34	2.2752162213815719E-4	0.0	0.0	12.294737576561026	0.0
35	2.2752162213815719E-4	0.0	0.0	12.610537588088787	0.0
36	3.033621628508763E-4	0.0	0.0	12.911472853636857	0.0
37	3.033621628508763E-4	0.0	0.0	13.216200146220562	0.0
38	3.033621628508763E-4	0.0	0.0	13.55141533617078	0.0
39	3.033621628508763E-4	0.0	0.0	13.965732210084365	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTAAG	30	2.1657452E-6	45.000004	1
TAACGCA	220	0.0	39.886364	26
GCGATAT	80	0.0	39.375	9
TCTAACG	230	0.0	39.130436	24
TCGCAAT	70	0.0	38.571426	22
TACGGGT	150	0.0	37.500004	4
ATCTCGA	25	0.0021072207	36.0	11
CGAACTA	25	0.0021072207	36.0	24
CACGGAT	25	0.0021072207	36.0	22
ACGTAGT	25	0.0021072207	36.0	23
TGTCGAA	25	0.0021072207	36.0	40
CGCACGG	195	0.0	34.615383	2
GGGCGAT	2440	0.0	34.30328	7
ACGTACG	60	6.4337655E-9	33.750004	1
CTCACGA	40	1.5607407E-5	33.75	24
CATGCGG	260	0.0	33.75	2
CGTAAGG	235	0.0	33.51064	2
CGTATGG	175	0.0	33.42857	2
ACGGGTA	155	0.0	33.387096	5
TAGGGCA	615	0.0	33.292683	5
>>END_MODULE