FastQCFastQC Report
Sat 18 Jun 2016
SRR3552600_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552600_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1552227
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95910.6178864302708302No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC19980.12871828669389207No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC17810.11473837267358447No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG16760.10797389814762917No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT16660.10732966247849057Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG302.1659962E-645.0000041
TAACGGC207.034051E-445.09
TAACACG351.2123019E-745.01
CGTTTTT96900.042.9798741
AATTGCG603.6379788E-1241.2500041
TTAGGCG501.0822987E-940.51
TGCGTCG403.459263E-739.3751
TAACGGG4250.038.647063
AGTCGAT356.2497056E-638.57142627
AGTACGG1900.037.8947372
TATTACG301.1401062E-437.5000041
ATAGTCG301.1401062E-437.5000041
TACGAAT2200.036.8181812
CGAATAT2150.036.62790714
TGCGAAG1050.036.428571
GAGTACG751.8189894E-1236.01
ATACGGG4200.035.8928573
GTTTTTT120300.035.5174562
AGGGCGC2600.035.480776
GCTACGA2350.035.42553310
Produced by FastQC (version 0.11.5)