##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552600_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1552227
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67720893915645	31.0	31.0	34.0	30.0	34.0
2	31.82476983070131	33.0	31.0	34.0	30.0	34.0
3	31.808196868112717	33.0	31.0	34.0	30.0	34.0
4	35.53254453118004	37.0	35.0	37.0	33.0	37.0
5	35.44092262278649	37.0	35.0	37.0	33.0	37.0
6	35.381183937658605	37.0	35.0	37.0	33.0	37.0
7	35.80557611741066	37.0	35.0	37.0	35.0	37.0
8	35.696888406141625	37.0	35.0	37.0	35.0	37.0
9	37.49749488960055	39.0	37.0	39.0	35.0	39.0
10	36.84388430300465	39.0	37.0	39.0	32.0	39.0
11	36.778828740899364	39.0	37.0	39.0	32.0	39.0
12	36.77006713579908	39.0	35.0	39.0	32.0	39.0
13	36.70190700200422	39.0	37.0	39.0	32.0	39.0
14	37.85170081437831	40.0	37.0	41.0	33.0	41.0
15	37.98205030578646	40.0	37.0	41.0	33.0	41.0
16	38.00440270656289	40.0	37.0	41.0	33.0	41.0
17	37.967494445077946	40.0	37.0	41.0	33.0	41.0
18	37.88627565426964	39.0	37.0	41.0	33.0	41.0
19	37.747725042793355	39.0	37.0	41.0	33.0	41.0
20	37.59783523930456	39.0	35.0	41.0	33.0	41.0
21	37.45554033011924	39.0	35.0	41.0	32.0	41.0
22	37.519460104739835	39.0	35.0	41.0	33.0	41.0
23	33.72590864609364	39.0	35.0	41.0	8.0	41.0
24	35.27694531792064	39.0	35.0	41.0	18.0	41.0
25	36.262138205301156	38.0	34.0	40.0	29.0	41.0
26	36.83894365965803	38.0	35.0	40.0	31.0	41.0
27	30.97550487138801	38.0	32.0	40.0	0.0	41.0
28	33.406648640952646	38.0	32.0	40.0	16.0	41.0
29	35.2274428933397	38.0	32.0	40.0	25.0	41.0
30	36.103910059546706	38.0	35.0	40.0	30.0	41.0
31	36.416377888027974	38.0	35.0	40.0	30.0	41.0
32	34.899523072334134	38.0	35.0	40.0	25.0	41.0
33	35.341634954165855	38.0	35.0	40.0	23.0	41.0
34	35.64001914668409	39.0	35.0	40.0	26.0	41.0
35	35.75792587037849	39.0	35.0	40.0	28.0	41.0
36	35.740814326770504	39.0	35.0	40.0	27.0	41.0
37	35.75259997410173	39.0	35.0	40.0	27.0	41.0
38	35.704002700635925	39.0	35.0	40.0	27.0	41.0
39	35.661947640390224	39.0	35.0	40.0	27.0	41.0
40	35.61444427909062	39.0	35.0	40.0	27.0	41.0
41	35.525298812609236	38.0	35.0	40.0	26.0	41.0
42	35.462346035728025	38.0	35.0	40.0	26.0	41.0
43	35.43280267641266	38.0	35.0	40.0	26.0	41.0
44	35.36777223949848	38.0	35.0	40.0	26.0	41.0
45	35.28759002388182	38.0	34.0	40.0	26.0	41.0
46	35.183909956468995	38.0	34.0	40.0	26.0	41.0
47	35.09310493890391	38.0	34.0	40.0	25.0	41.0
48	35.03829723358761	38.0	34.0	40.0	25.0	41.0
49	35.003152245129094	38.0	34.0	40.0	25.0	41.0
50	34.87301148607774	38.0	34.0	40.0	24.0	41.0
51	34.14021467220967	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	7.0
9	43.0
10	72.0
11	69.0
12	67.0
13	51.0
14	72.0
15	82.0
16	162.0
17	397.0
18	800.0
19	1528.0
20	2724.0
21	4407.0
22	6722.0
23	9443.0
24	13442.0
25	18841.0
26	23928.0
27	26316.0
28	27568.0
29	31119.0
30	38034.0
31	47751.0
32	61202.0
33	81354.0
34	115134.0
35	130958.0
36	141249.0
37	179117.0
38	258087.0
39	331334.0
40	143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.18065656633985	18.298418981244367	22.999728776783293	18.521195675632494
2	34.98103048072221	23.199635104917	25.54639237688817	16.272942037472614
3	29.793644872818213	23.31546867822812	30.872997312893023	16.01788913606064
4	26.57826464814747	25.390036379988235	29.888669634016157	18.143029337848137
5	23.77757892370124	29.307440213319314	29.30750463688623	17.60747622609322
6	22.550245550425295	34.4022491555681	28.934943149423376	14.112562144583235
7	78.90579148539486	5.435609611223101	11.768510662422441	3.890088240959602
8	81.38648535297995	4.031691241036266	10.835270872108268	3.746552533875522
9	75.51086277973518	6.521211137288553	13.05047522044134	4.917450862534926
10	35.50698448100697	24.068773446151884	23.723785245328163	16.700456827512987
11	27.512535215532264	26.505852558936287	26.99643802098533	18.985174204546112
12	22.73494791676733	23.722174656155318	33.352016167738356	20.190861259339002
13	22.339709333750797	24.45441291769825	34.66876945189073	18.537108296660218
14	20.912598479474973	26.261944934600418	33.39472899260224	19.43072759332237
15	20.696843953880457	26.16299033582073	34.38865578294927	18.751509927349545
16	22.001227913185378	24.765772016592933	33.57472843855957	19.65827163166212
17	22.908505006033266	25.102127459450195	31.226167306714803	20.763200227801732
18	22.903930932782384	24.55993872030315	32.568754441199644	19.96737590571482
19	22.5595869676278	26.858764858490414	30.9068196855228	19.674828488358983
20	22.372372082176124	26.16634036130025	31.78864946943972	19.672638087083914
21	22.249387492937565	26.69680401126897	31.919687004542507	19.13412149125096
22	21.28522439050474	25.19534836077455	31.289753367258783	22.229673881461924
23	18.47339338898241	33.83184289411278	27.88477458516055	19.809989131744263
24	20.0439755267754	25.90362105542553	32.53512533927061	21.517278078528463
25	20.572184351902138	27.30412497656593	30.869389593145847	21.25430107838609
26	20.387353138426274	27.226816696269296	31.603947103097678	20.781883062206752
27	17.90891409568317	38.25278132644259	26.171429823086445	17.6668747547878
28	20.25025978803358	26.252410246697167	32.53267724372788	20.96465272154137
29	21.93867262971202	24.93043865362476	31.256317536030494	21.874571180632728
30	21.536862842870278	25.85227547259518	31.536431204971954	21.07443047956259
31	21.809116836648247	25.8951171445929	30.44380751011289	21.851958508645968
32	21.559411091290126	28.55613257596988	29.175565171846646	20.708891160893348
33	21.93448509786262	25.761824784648123	30.006951302870004	22.296738814619253
34	21.088410393582897	25.838939794244016	30.53696398787033	22.53568582430276
35	22.934145585664982	24.6717136088987	30.54044286048368	21.85369794495264
36	22.585163123692602	26.344858065218553	29.657775570196886	21.41220324089196
37	21.9376418526414	27.09687436180404	30.107645337956367	20.857838447598194
38	21.5890459320705	27.32216357530181	29.72561358615718	21.36317690647051
39	21.998457699808082	26.509331431549636	30.315926729788877	21.1762841388534
40	22.941683142993906	25.471210074299698	30.749304064418414	20.837802718287982
41	20.811646750120953	26.413469163981812	30.172326599137882	22.60255748675935
42	21.778257948096506	26.631091973016837	29.540073713445263	22.050576365441398
43	21.471601769586535	25.831724354749657	30.71554611535555	21.981127760308254
44	21.767177094587325	26.106426444070358	29.375084958578867	22.75131150276345
45	22.05998220621082	25.514889252667295	29.763752337770182	22.6613762033517
46	22.28263005346512	26.390920915561967	29.431262308927753	21.895186722045164
47	21.50142988106765	26.3376426257242	30.480400096119965	21.680527397088184
48	21.60598933016885	25.667444259119314	30.164080382572912	22.562486028138924
49	21.801836973586983	25.36452464749035	30.113894423947013	22.71974395497566
50	20.799084154572753	25.21957162193416	31.002810800224452	22.978533423268633
51	20.899262801123804	24.699222471970916	29.453166321678463	24.948348405226813
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1783.0
1	2138.0
2	2493.0
3	11398.0
4	20303.0
5	14285.5
6	8268.0
7	8212.5
8	8157.0
9	8333.5
10	8510.0
11	8570.0
12	8630.0
13	8577.5
14	8525.0
15	8345.0
16	8165.0
17	8056.0
18	7947.0
19	7989.5
20	8032.0
21	7862.5
22	7693.0
23	8478.0
24	9263.0
25	10455.5
26	13674.5
27	15701.0
28	18317.5
29	20934.0
30	24392.5
31	27851.0
32	31343.5
33	34836.0
34	39617.0
35	44398.0
36	49601.0
37	54804.0
38	59815.5
39	64827.0
40	70466.0
41	76105.0
42	83362.5
43	90620.0
44	99285.5
45	107951.0
46	119965.5
47	131980.0
48	138797.0
49	145614.0
50	143764.5
51	141915.0
52	127509.0
53	113103.0
54	100479.0
55	87855.0
56	79306.5
57	70758.0
58	63281.5
59	55805.0
60	49426.5
61	43048.0
62	38479.5
63	33911.0
64	28919.0
65	23927.0
66	19728.5
67	15530.0
68	13556.5
69	11583.0
70	9411.0
71	7239.0
72	6158.0
73	5077.0
74	4211.0
75	2649.0
76	1953.0
77	1544.0
78	1135.0
79	881.0
80	627.0
81	430.0
82	233.0
83	161.5
84	90.0
85	58.5
86	27.0
87	17.5
88	8.0
89	8.5
90	9.0
91	8.0
92	7.0
93	5.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1552227.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.51055212351506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.07214194858174	27.013637618125486
2	9.006022736000634	6.396176795846245
3	3.441936052391196	3.6667514878272964
4	1.8447731372934741	2.6203565059168126
5	1.215821613282506	2.15872483856823
6	0.864142968276271	1.8411716350286125
7	0.7174134386560718	1.783302311525463
8	0.5876829984450004	1.6695158198707842
9	0.5162195872001045	1.6498118302603864
>10	5.403596541551709	40.8653940671013
>50	0.29388816270602747	6.47414373770428
>100	0.03301955363880433	1.9109647589706087
>500	0.0015723585771253987	0.4129719944984821
>1k	0.0015723585771253987	0.86768089160134
>5k	1.9654482214067484E-4	0.6693957071547983
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9591	0.6178864302708302	No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	1998	0.12871828669389207	No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	1781	0.11473837267358447	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG	1676	0.10797389814762917	No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	1666	0.10732966247849057	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.288471338277198E-4	0.0	0.0	0.12949136949685838	0.0
2	1.288471338277198E-4	0.0	0.0	0.5098481085562871	0.0
3	1.288471338277198E-4	0.0	0.0	0.7923454494735628	0.0
4	1.288471338277198E-4	0.0	0.0	1.0327742011960879	0.0
5	1.288471338277198E-4	0.0	0.0	1.7498729245142624	0.0
6	1.288471338277198E-4	0.0	0.0	2.5031132688711124	0.0
7	1.288471338277198E-4	0.0	0.0	3.1660961959816443	0.0
8	1.288471338277198E-4	0.0	0.0	4.211626263426677	0.0
9	1.288471338277198E-4	0.0	0.0	4.709878129938469	0.0
10	1.288471338277198E-4	0.0	0.0	5.706059745127485	0.0
11	1.288471338277198E-4	0.0	0.0	6.840558758480557	0.0
12	1.288471338277198E-4	0.0	0.0	7.772703348157196	0.0
13	1.288471338277198E-4	0.0	0.0	8.183790128634536	0.0
14	1.288471338277198E-4	0.0	0.0	8.388528224286782	0.0
15	1.9327070074157967E-4	0.0	0.0	8.638749358180215	0.0
16	1.9327070074157967E-4	0.0	0.0	9.13738776609349	0.0
17	1.9327070074157967E-4	0.0	0.0	9.687307333270198	0.0
18	1.9327070074157967E-4	0.0	0.0	10.339724795406857	0.0
19	1.9327070074157967E-4	0.0	0.0	10.68677455037182	0.0
20	1.9327070074157967E-4	0.0	0.0	11.016043400868558	0.0
21	1.9327070074157967E-4	0.0	0.0	11.432541760966663	0.0
22	2.576942676554396E-4	0.0	0.0	11.876420137003157	0.0
23	2.576942676554396E-4	0.0	0.0	12.3326034143202	0.0
24	2.576942676554396E-4	0.0	0.0	12.704327395413172	0.0
25	3.221178345692995E-4	0.0	0.0	13.039007825530673	0.0
26	3.221178345692995E-4	0.0	0.0	13.358677564557246	0.0
27	3.221178345692995E-4	0.0	0.0	13.681955023330994	0.0
28	3.221178345692995E-4	0.0	0.0	14.069398354750948	0.0
29	3.221178345692995E-4	0.0	0.0	14.45516667343114	0.0
30	3.8654140148315934E-4	0.0	0.0	14.904972017623711	0.0
31	3.8654140148315934E-4	0.0	0.0	15.322307884091696	0.0
32	3.8654140148315934E-4	0.0	0.0	15.742671658204632	0.0
33	3.8654140148315934E-4	0.0	0.0	16.16361524441979	0.0
34	3.8654140148315934E-4	0.0	0.0	16.566906773300555	0.0
35	3.8654140148315934E-4	0.0	0.0	16.983662827666315	0.0
36	3.8654140148315934E-4	0.0	0.0	17.397584245087863	0.0
37	3.8654140148315934E-4	0.0	0.0	17.808799872699034	0.0
38	4.5096496839701925E-4	0.0	0.0	18.23689447484163	0.0
39	4.5096496839701925E-4	0.0	0.0	18.65345725850665	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGACG	30	2.1659962E-6	45.000004	1
TAACGGC	20	7.034051E-4	45.0	9
TAACACG	35	1.2123019E-7	45.0	1
CGTTTTT	9690	0.0	42.979874	1
AATTGCG	60	3.6379788E-12	41.250004	1
TTAGGCG	50	1.0822987E-9	40.5	1
TGCGTCG	40	3.459263E-7	39.375	1
TAACGGG	425	0.0	38.64706	3
AGTCGAT	35	6.2497056E-6	38.571426	27
AGTACGG	190	0.0	37.894737	2
TATTACG	30	1.1401062E-4	37.500004	1
ATAGTCG	30	1.1401062E-4	37.500004	1
TACGAAT	220	0.0	36.81818	12
CGAATAT	215	0.0	36.627907	14
TGCGAAG	105	0.0	36.42857	1
GAGTACG	75	1.8189894E-12	36.0	1
ATACGGG	420	0.0	35.892857	3
GTTTTTT	12030	0.0	35.517456	2
AGGGCGC	260	0.0	35.48077	6
GCTACGA	235	0.0	35.425533	10
>>END_MODULE