##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552596_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	520174
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.871239239177658	33.0	31.0	34.0	30.0	34.0
2	32.00014610495719	33.0	31.0	34.0	30.0	34.0
3	32.01643873011723	33.0	31.0	34.0	30.0	34.0
4	35.683034907550166	37.0	35.0	37.0	33.0	37.0
5	35.571710619907954	37.0	35.0	37.0	33.0	37.0
6	35.51293797844568	37.0	35.0	37.0	33.0	37.0
7	35.82817672548032	37.0	35.0	37.0	35.0	37.0
8	35.656341916358755	37.0	35.0	37.0	35.0	37.0
9	37.49593789770346	39.0	37.0	39.0	35.0	39.0
10	37.04173795691442	39.0	37.0	39.0	33.0	39.0
11	36.901469508279924	39.0	37.0	39.0	33.0	39.0
12	36.74345891951539	39.0	35.0	39.0	33.0	39.0
13	36.57017844029113	39.0	35.0	39.0	32.0	39.0
14	37.71532218065494	40.0	36.0	41.0	33.0	41.0
15	37.893762471788285	40.0	36.0	41.0	33.0	41.0
16	37.948647952415925	40.0	36.0	41.0	33.0	41.0
17	37.918575322872734	40.0	36.0	41.0	33.0	41.0
18	37.9016559843437	40.0	36.0	41.0	33.0	41.0
19	37.80817380338117	40.0	36.0	41.0	33.0	41.0
20	37.71186372252362	40.0	36.0	41.0	33.0	41.0
21	37.55978384156071	39.0	35.0	41.0	33.0	41.0
22	37.604870677888556	39.0	35.0	41.0	33.0	41.0
23	33.62542149357715	39.0	35.0	41.0	0.0	41.0
24	35.20831106514358	39.0	35.0	41.0	18.0	41.0
25	36.28302452640847	39.0	34.0	41.0	29.0	41.0
26	36.901911668018776	39.0	35.0	41.0	31.0	41.0
27	30.80851407413673	38.0	32.0	40.0	0.0	41.0
28	33.301885522921175	38.0	32.0	40.0	16.0	41.0
29	35.24165375432067	38.0	32.0	40.0	25.0	41.0
30	36.231332208068835	38.0	35.0	40.0	30.0	41.0
31	36.60889048664485	38.0	35.0	40.0	31.0	41.0
32	35.09886884004199	38.0	35.0	40.0	27.0	41.0
33	35.726556882889184	38.0	35.0	40.0	27.0	41.0
34	36.16880120882628	38.0	35.0	40.0	30.0	41.0
35	36.33026448842118	38.0	35.0	41.0	30.0	41.0
36	36.30916001184219	38.0	35.0	40.0	30.0	41.0
37	36.33934798740422	38.0	35.0	40.0	30.0	41.0
38	36.27294328436254	38.0	35.0	40.0	30.0	41.0
39	36.23972939823982	38.0	35.0	40.0	30.0	41.0
40	36.13026410393445	38.0	35.0	40.0	30.0	41.0
41	36.01784210667969	38.0	35.0	40.0	30.0	41.0
42	35.96091692395237	38.0	35.0	40.0	30.0	41.0
43	35.931924702118906	38.0	35.0	40.0	30.0	41.0
44	35.85764955572559	38.0	35.0	40.0	30.0	41.0
45	35.780823339882424	38.0	35.0	40.0	30.0	41.0
46	35.67083322119137	38.0	35.0	40.0	29.0	41.0
47	35.56244064486114	37.0	34.0	40.0	29.0	41.0
48	35.53468454786283	37.0	34.0	40.0	29.0	41.0
49	35.49306578183454	37.0	34.0	40.0	29.0	41.0
50	35.368865033623365	37.0	34.0	40.0	29.0	41.0
51	34.63299588214713	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	29.0
10	38.0
11	41.0
12	35.0
13	34.0
14	36.0
15	61.0
16	74.0
17	148.0
18	251.0
19	443.0
20	726.0
21	1077.0
22	1656.0
23	2242.0
24	3079.0
25	4066.0
26	5139.0
27	6432.0
28	7727.0
29	9562.0
30	12494.0
31	16150.0
32	21037.0
33	28343.0
34	43099.0
35	51563.0
36	48402.0
37	60698.0
38	84087.0
39	111330.0
40	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.027909891690086	18.198141391149885	23.381983720831876	15.391964996328152
2	30.480762206492447	25.443986050821454	25.179843667695813	18.895408074990293
3	31.166109801720193	24.6969283355185	27.202628351282453	16.93433351147885
4	28.71539138826623	26.47710189282817	25.941511878717506	18.86599484018809
5	25.551834578429528	30.03091273304702	24.69019981775329	19.727052870770166
6	24.624452587018958	35.54233775621235	24.444512797640787	15.388696859127906
7	81.16668653181436	6.520510444582005	7.711073602294618	4.601729421309023
8	83.3763317659091	5.1905708474472005	7.1677938535951435	4.265303533048557
9	78.05811132428764	7.830841218515343	9.143478912825325	4.967568544371691
10	42.714553207196055	25.90114077212625	15.926593793615213	15.457712227062483
11	31.929892689753814	26.79334222779301	22.435569636314003	18.841195446139178
12	28.621576626282746	23.607100700919307	28.740959755774032	19.03036291702392
13	24.121351701546022	25.7204320092892	29.07142609972817	21.08679018943661
14	20.54735530803154	28.49046665154352	29.169854702464942	21.79232333795999
15	19.89507357153568	26.67357461157228	32.66945291383268	20.76189890305936
16	22.034549977507524	24.858028275154084	31.59596596523471	21.51145578210368
17	22.31849342719936	25.095641073948332	28.018316947790545	24.567548551061762
18	23.75224444128311	24.89724592155702	28.444520487375378	22.905989149784496
19	24.608111901017736	26.480370030028418	26.442305843813802	22.46921222514005
20	26.31292605935706	25.680445389427387	26.673190124842844	21.333438426372712
21	25.07237962681718	27.259340143874933	25.969579409966663	21.69870081934122
22	24.061371771753297	25.0583458611926	26.40116576376366	24.47911660329044
23	20.996051321288643	34.86852476286781	23.45061460203701	20.684809313806536
24	21.76483253680499	25.332677142648418	29.479366519664573	23.423123800882014
25	22.19045934629566	27.364305021012196	26.985393349148556	23.459842283543583
26	22.058964884827002	28.07772014748911	26.32311495768724	23.540200009996656
27	18.732001214978062	39.642888725695634	22.638578629458607	18.986531429867696
28	20.790927651132122	27.341812547339927	28.840157331969685	23.027102469558265
29	22.067615836239412	26.605904947190744	27.664204669975817	23.662274546594027
30	22.486898614694315	27.64498033350379	27.12784568240627	22.740275369395626
31	24.014848877490994	28.12558874530446	24.925697939535617	22.933864437668934
32	22.940208468704704	30.63071203097425	24.942615355631002	21.486464144690046
33	23.228381272420382	28.397613106383634	24.485268390961487	23.8887372302345
34	22.222756231568667	27.761095325794834	26.415199529388246	23.60094891324826
35	22.746619400431396	26.92925828665024	26.318501116933952	24.00562119598442
36	23.40486068123359	28.742113215962352	25.362090377450624	22.490935725353438
37	22.92271432251516	28.86245756227724	25.813093311084366	22.401734804123237
38	22.287542245479397	29.261939274165954	25.8513497406637	22.59916873969095
39	23.75493584838919	27.798775025280005	25.54510606066432	22.90118306566649
40	24.30129149092419	26.44115238362548	27.934883327501954	21.32267279794838
41	21.77828957233541	27.086705602356137	27.184941961728192	23.950062863580264
42	21.95130860058365	27.23184934271994	27.25799444032189	23.55884761637452
43	22.688754147650595	26.261212594247308	27.455812862619045	23.594220395483052
44	21.983413242491938	26.856590294785974	26.99077616336072	24.169220299361367
45	22.56418044731186	26.69279894804431	26.494403795652993	24.24861680899084
46	22.06665461941581	28.41087789854933	26.466336264403832	23.056131217631023
47	21.854610188129357	27.33566075966888	28.419336606597025	22.39039244560474
48	22.119137057984446	26.085694402257708	28.204024038110326	23.591144501647527
49	22.243903001687897	26.616478332250367	28.13596988699935	23.00364877906239
50	21.914013387827918	26.81660367492416	27.404483884238733	23.864899053009186
51	21.058530414822734	26.71567590844602	26.870047330316392	25.355746346414854
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1547.0
1	1804.0
2	2061.0
3	2790.5
4	3520.0
5	2528.5
6	1537.0
7	1542.5
8	1548.0
9	1566.5
10	1585.0
11	1629.5
12	1674.0
13	1649.5
14	1625.0
15	1623.0
16	1621.0
17	1715.5
18	1810.0
19	1812.0
20	1814.0
21	1836.0
22	1858.0
23	2126.5
24	2395.0
25	2998.0
26	4135.0
27	4669.0
28	5992.5
29	7316.0
30	8494.5
31	9673.0
32	9944.5
33	10216.0
34	11814.5
35	13413.0
36	14784.0
37	16155.0
38	17535.0
39	18915.0
40	21084.0
41	23253.0
42	26043.0
43	28833.0
44	32586.0
45	36339.0
46	38563.0
47	40787.0
48	40830.0
49	40873.0
50	38103.5
51	35334.0
52	33278.0
53	31222.0
54	29682.5
55	28143.0
56	26958.0
57	25773.0
58	24883.5
59	23994.0
60	23516.0
61	23038.0
62	21613.5
63	20189.0
64	17619.0
65	15049.0
66	13032.0
67	11015.0
68	10077.5
69	9140.0
70	7804.5
71	6469.0
72	5446.5
73	4424.0
74	3924.0
75	2725.5
76	2027.0
77	1618.0
78	1209.0
79	961.0
80	713.0
81	489.0
82	265.0
83	162.5
84	60.0
85	47.0
86	34.0
87	21.5
88	9.0
89	5.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	520174.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.478590966657656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.38443186090349	24.159015366975016
2	9.438271057344851	6.130834902078937
3	3.809828446322995	3.7121357928378416
4	2.0535578718664937	2.667866645868473
5	1.2245071732589123	1.9885133808007205
6	0.8529211633400529	1.662100655455643
7	0.6857390202519759	1.5590286004048344
8	0.5071367717376389	1.3176870218734402
9	0.43226971647990176	1.2635560177941425
>10	6.2665739221825	46.724640188352815
>50	0.3165230239171076	6.403355271166272
>100	0.024709975844829958	1.3756006862560348
>500	0.0023533310328409484	0.503501570813259
>1k	0.0011766655164204742	0.5321638993225527
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1428	0.2745235248205408	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1357	0.26087424592540187	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	690	0.13264792165698402	No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	685	0.13168670483338268	TruSeq Adapter, Index 21 (95% over 21bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	650	0.12495818706817334	No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	610	0.11726845247936267	TruSeq Adapter, Index 21 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.922433647202667E-4	0.0	0.0	0.08497156720635787	0.0
2	1.922433647202667E-4	0.0	0.0	0.455616774387032	0.0
3	1.922433647202667E-4	0.0	0.0	0.6130640900929304	0.0
4	1.922433647202667E-4	0.0	0.0	0.8079988619192808	0.0
5	1.922433647202667E-4	0.0	0.0	1.3182127518868687	0.0
6	1.922433647202667E-4	0.0	0.0	1.7628716544848455	0.0
7	1.922433647202667E-4	0.0	0.0	2.2090685040005846	0.0
8	1.922433647202667E-4	0.0	0.0	3.0201432597553897	0.0
9	1.922433647202667E-4	0.0	0.0	3.393095387312707	0.0
10	1.922433647202667E-4	0.0	0.0	4.118045115672833	0.0
11	1.922433647202667E-4	0.0	0.0	4.989292044585081	0.0
12	1.922433647202667E-4	0.0	0.0	5.695978653296781	0.0
13	1.922433647202667E-4	0.0	0.0	5.963389173622672	0.0
14	1.922433647202667E-4	0.0	0.0	6.087193900502524	0.0
15	1.922433647202667E-4	0.0	0.0	6.2431032692906605	0.0
16	1.922433647202667E-4	0.0	0.0	6.583950754939694	0.0
17	1.922433647202667E-4	0.0	0.0	6.947675200990438	0.0
18	1.922433647202667E-4	0.0	0.0	7.406752355942435	0.0
19	1.922433647202667E-4	0.0	0.0	7.639751313983398	0.0
20	1.922433647202667E-4	0.0	0.0	7.874672705671564	0.0
21	3.844867294405334E-4	0.0	0.0	8.132086571032001	0.0
22	3.844867294405334E-4	0.0	0.0	8.418144697735757	0.0
23	3.844867294405334E-4	0.0	0.0	8.71496845286385	0.0
24	3.844867294405334E-4	0.0	0.0	8.95431144194058	0.0
25	3.844867294405334E-4	0.0	0.0	9.16308773602679	0.0
26	3.844867294405334E-4	0.0	0.0	9.360906158323946	0.0
27	3.844867294405334E-4	0.0	0.0	9.548727925655646	0.0
28	3.844867294405334E-4	0.0	0.0	9.773075932284197	0.0
29	3.844867294405334E-4	0.0	0.0	10.017225005478936	0.0
30	3.844867294405334E-4	0.0	0.0	10.316740167713112	0.0
31	3.844867294405334E-4	0.0	0.0	10.578383387097395	0.0
32	3.844867294405334E-4	0.0	0.0	10.839449876387517	0.0
33	3.844867294405334E-4	0.0	0.0	11.106860396713408	0.0
34	3.844867294405334E-4	0.0	0.0	11.36811912936825	0.0
35	3.844867294405334E-4	0.0	0.0	11.667634291602425	0.0
36	7.689734588810668E-4	0.0	0.0	11.941196599599365	0.0
37	7.689734588810668E-4	0.0	0.0	12.245710089316267	0.0
38	7.689734588810668E-4	0.0	0.0	12.531960459384745	0.0
39	7.689734588810668E-4	0.0	0.0	12.818210829453221	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAAT	20	7.0297206E-4	45.000004	22
CAACGGA	20	7.0297206E-4	45.000004	3
GCAACGC	20	7.0297206E-4	45.000004	13
CAAATCG	20	7.0297206E-4	45.000004	1
GCGATAT	20	7.0297206E-4	45.000004	9
CAGTACG	20	7.0297206E-4	45.000004	1
ATCGCTT	20	7.0297206E-4	45.000004	25
GTACGCG	20	7.0297206E-4	45.000004	1
TTATCGC	25	3.8878516E-5	45.0	24
CTAACGG	25	3.8878516E-5	45.0	2
TTCGGAA	25	3.8878516E-5	45.0	32
TATGTCG	25	3.8878516E-5	45.0	5
CGATAGA	25	3.8878516E-5	45.0	10
TCAACGA	25	3.8878516E-5	45.0	21
GTAAGCG	30	2.1632022E-6	44.999996	1
CCAATCT	60	3.6379788E-12	41.249996	16
TCGCGCG	45	1.9248546E-8	40.000004	1
GATAACG	40	3.4533514E-7	39.375004	1
TACGCAT	115	0.0	39.130436	16
GGCACAT	75	0.0	39.0	8
>>END_MODULE