FastQCFastQC Report
Sat 18 Jun 2016
SRR3552595_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552595_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726250
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27630.38044750430292595No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18320.25225473321858866No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC11100.15283993115318417Illumina PCR Primer Index 5 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGC10690.14719449225473322No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10670.14691910499139416No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC10230.1408605851979346No Hit
GAATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC10080.13879518072289157No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC9020.12419965576592083No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCG8610.11855421686746986No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAAC253.8893828E-545.010
GCGCATC207.03157E-445.018
TTATCGC253.8893828E-545.015
ATCGTTC207.03157E-445.032
ATAACGG253.8893828E-545.02
CAACTCG207.03157E-445.015
CGATCCA207.03157E-445.010
TATCGTG302.1643937E-644.99999637
TCGATAG700.041.7857131
ATCCGGC650.041.5384646
CGTGTAC1800.041.2518
TTGTGCG501.0804797E-940.51
TCGTGTA1850.040.13513617
CGACAAT900.040.020
ATCGGGT451.9266736E-840.04
TCGACAA900.040.019
TGCGTAG451.9266736E-840.01
CATACGA1250.039.618
ACATACG1250.039.617
ACGGGAT2050.039.5121965