FastQCFastQC Report
Sat 18 Jun 2016
SRR3552594_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552594_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786059
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22010.2800044271485983No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19950.25379774291751633No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC10730.13650374844636345No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC9220.11729399447115293No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9070.11538574076500618No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC8040.10228239864946524No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT7930.10088301259829097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACAA302.1646229E-645.00000419
CGAACTA253.889677E-545.016
GGTCGTA207.0319243E-445.044
CGTACTC207.0319243E-445.045
TTACGCG253.889677E-545.01
CCCGATA207.0319243E-445.021
GCGAATG650.041.538461
TTAACGG650.041.538462
GCGATAC650.041.538469
TCGATGG451.9270374E-840.02
CTAACGC1700.039.70588325
ACGCATT1650.039.54545217
TTTAGCG800.039.3751
TTAGCGG1600.039.3752
ATGACGC1500.039.00000427
TACGCAT1800.038.7516
GGTCCGT356.2457693E-638.5714268
AGGGCGT700.038.5714266
ACGAGAT700.038.57142615
TAACGCA1700.038.38235526