##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552594_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	786059
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.715542980870392	31.0	31.0	34.0	30.0	34.0
2	31.834906794528145	33.0	31.0	34.0	30.0	34.0
3	31.87348405145161	33.0	31.0	34.0	30.0	34.0
4	35.550442142383716	37.0	35.0	37.0	33.0	37.0
5	35.44436613536643	37.0	35.0	37.0	33.0	37.0
6	35.38927612303911	37.0	35.0	37.0	33.0	37.0
7	35.75287351203917	37.0	35.0	37.0	35.0	37.0
8	35.58747116946692	37.0	35.0	37.0	35.0	37.0
9	37.41211283122514	39.0	37.0	39.0	35.0	39.0
10	36.86513862191006	39.0	37.0	39.0	32.0	39.0
11	36.74228143180092	39.0	37.0	39.0	32.0	39.0
12	36.638024626650164	39.0	35.0	39.0	32.0	39.0
13	36.47440459303945	39.0	35.0	39.0	32.0	39.0
14	37.584134269819444	39.0	36.0	41.0	33.0	41.0
15	37.75002894184788	40.0	36.0	41.0	33.0	41.0
16	37.80920643361376	40.0	36.0	41.0	33.0	41.0
17	37.75422073915571	40.0	36.0	41.0	33.0	41.0
18	37.73491302815692	39.0	36.0	41.0	33.0	41.0
19	37.65052750493284	39.0	36.0	41.0	33.0	41.0
20	37.56059786860783	39.0	35.0	41.0	33.0	41.0
21	37.40283490170585	39.0	35.0	41.0	32.0	41.0
22	37.46673214097161	39.0	35.0	41.0	33.0	41.0
23	33.44996495174026	39.0	34.0	41.0	0.0	41.0
24	35.06256782251714	38.0	34.0	41.0	18.0	41.0
25	36.08499743657919	38.0	34.0	40.0	27.0	41.0
26	36.68860098287787	38.0	35.0	40.0	31.0	41.0
27	30.570606023211997	38.0	31.0	40.0	0.0	41.0
28	33.11349148091937	38.0	31.0	40.0	16.0	41.0
29	35.0370252105758	38.0	32.0	40.0	25.0	41.0
30	36.01659417422865	38.0	34.0	40.0	30.0	41.0
31	36.40172302587974	38.0	35.0	40.0	30.0	41.0
32	34.898019105436106	38.0	35.0	40.0	26.0	41.0
33	35.53958545096488	38.0	35.0	40.0	25.0	41.0
34	35.94856238526624	38.0	35.0	40.0	29.0	41.0
35	36.13046348938184	38.0	35.0	40.0	30.0	41.0
36	36.169731534146926	38.0	35.0	40.0	30.0	41.0
37	36.16369636375895	38.0	35.0	40.0	30.0	41.0
38	36.093972589843766	38.0	35.0	40.0	30.0	41.0
39	36.03447323928611	38.0	35.0	40.0	30.0	41.0
40	35.946617238655115	38.0	35.0	40.0	30.0	41.0
41	35.860238226392674	38.0	35.0	40.0	29.0	41.0
42	35.8120726306804	38.0	35.0	40.0	29.0	41.0
43	35.77013430289584	38.0	35.0	40.0	29.0	41.0
44	35.69001817929697	38.0	35.0	40.0	29.0	41.0
45	35.594254375307706	38.0	34.0	40.0	29.0	41.0
46	35.492917198327355	38.0	34.0	40.0	28.0	41.0
47	35.387568872056676	37.0	34.0	40.0	28.0	41.0
48	35.341270820638144	37.0	34.0	40.0	28.0	41.0
49	35.30693879212629	37.0	34.0	40.0	28.0	41.0
50	35.20679872630426	37.0	34.0	40.0	28.0	41.0
51	34.47563096408794	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	37.0
10	52.0
11	62.0
12	36.0
13	54.0
14	59.0
15	84.0
16	115.0
17	225.0
18	409.0
19	750.0
20	1283.0
21	1936.0
22	2829.0
23	3828.0
24	5167.0
25	6418.0
26	8455.0
27	10329.0
28	12448.0
29	16027.0
30	20319.0
31	25845.0
32	33311.0
33	44579.0
34	66250.0
35	77783.0
36	73507.0
37	92899.0
38	125830.0
39	155037.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.9958298295675	19.27781502406308	23.77035311598748	14.956002030381942
2	30.55801154875143	25.063767478013737	26.611234016785	17.766986956449834
3	31.083799053251727	25.095826140277	27.07544853503363	16.74492627143764
4	27.758985012575394	27.10114635160974	25.927061454674522	19.21280718114035
5	24.497397778029384	30.781531666198088	25.496050550912848	19.225020004859687
6	24.443839457343532	35.78128359321629	24.943801928353977	14.831075021086203
7	81.07215870564424	6.999220160318755	7.491040748849641	4.437580385187371
8	83.48559077626489	5.442594003757987	6.793001543141164	4.278813676835963
9	78.37261579601531	7.786565639474899	8.796031850026525	5.044786714483264
10	42.16642771089702	25.683441064856456	16.387828394560714	15.762302829685812
11	31.14435430419345	27.60848740361729	22.2989622916346	18.948196000554667
12	27.296551531119167	23.761193498197972	28.950371409779674	19.99188356090319
13	23.58716076019739	26.650544043131625	29.392704618864485	20.369590577806502
14	20.71218572651671	29.440156527690668	28.63818110345407	21.20947664233855
15	20.424039416888554	26.163557697322975	33.03225330414129	20.380149581647178
16	22.493604169661566	24.781854797158992	31.255669103718674	21.468871929460764
17	22.024809842518184	24.95168937700605	27.9667302327179	25.056770547757868
18	23.805464984180578	24.248434277834107	29.179489071431025	22.766611666554294
19	24.278966337132456	26.410612943812104	27.413591091762836	21.896829627292608
20	25.715754160947206	25.82986773257478	26.67662351044896	21.77775459602905
21	23.612731359859758	27.687616323965504	27.124935914479703	21.57471640169504
22	22.23331836414315	26.10745503836226	26.542536883363717	25.116689714130874
23	19.485178593464358	34.88147836231123	23.700892681083737	21.932450363140678
24	20.860775081768672	26.607290292458963	29.97497643306673	22.556958192705636
25	21.33071436113574	28.309070947600624	27.350109851805016	23.010104839458613
26	20.944611027925387	28.73804638074241	27.168698532807333	23.14864405852487
27	17.840899983334584	39.99470777638829	22.571715354699837	19.592676885577294
28	20.210696652541348	27.152287550934474	29.330622764957848	23.306393031566333
29	21.68412294751412	26.10618286922483	28.031992509468118	24.177701673792935
30	22.43877367983828	27.352908623907368	26.96273434945723	23.245583346797126
31	24.379340482075772	27.447685224645983	24.87485036110521	23.29812393217303
32	22.611915899442664	31.104280976364368	23.77735004624335	22.50645307794962
33	23.327765473075175	27.029014361517394	25.488671969915742	24.15454819549169
34	22.101648858419022	26.31927119974455	27.520707733134536	24.058372208701893
35	21.99135179420374	26.604364303442875	26.597367373186998	24.806916529166386
36	23.858896087952687	27.43788952228777	25.900727553529695	22.802486836229853
37	21.96629006219635	28.395578448945948	27.32415760140142	22.313973887456285
38	22.751600070732604	28.421149048608314	26.28823027279123	22.539020607867858
39	23.18630026499283	27.160938301069002	27.307492185701072	22.34526924823709
40	23.498363354404695	25.845388196051438	28.18872374719964	22.467524702344228
41	20.697301347608768	26.22640285271207	27.117302899655115	25.958992900024043
42	21.592526769619074	25.728730286149005	27.16615419453247	25.51258874969945
43	21.842762438951784	25.15320096837515	28.387436566466384	24.616600026206683
44	22.504672041157217	25.579632063242073	27.441706029700057	24.47398986590065
45	22.508361331655767	26.615941042593494	26.92367875693809	23.952018868812647
46	22.419309492035584	26.76122275808813	27.323012649177734	23.496455100698547
47	21.75320173167663	26.1686463738727	28.828370389500023	23.249781504950644
48	21.549145802032672	25.02712900685572	29.035733958901304	24.387991232210304
49	22.393738892373218	25.283216654220613	29.270958032412324	23.052086420993845
50	21.873930582818847	25.29135853670017	28.495570943148035	24.33913993733295
51	21.28517070601571	25.374812832115655	26.95942670969991	26.38058975216873
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2221.0
1	2446.0
2	2671.0
3	3871.5
4	5072.0
5	3657.0
6	2242.0
7	2203.5
8	2165.0
9	2325.5
10	2486.0
11	2459.5
12	2433.0
13	2578.0
14	2723.0
15	2681.0
16	2639.0
17	2508.0
18	2377.0
19	2436.0
20	2495.0
21	2747.5
22	3000.0
23	3453.0
24	3906.0
25	4495.5
26	6090.5
27	7096.0
28	8271.5
29	9447.0
30	10951.5
31	12456.0
32	14306.5
33	16157.0
34	18819.5
35	21482.0
36	22813.0
37	24144.0
38	25877.5
39	27611.0
40	30417.5
41	33224.0
42	37495.0
43	41766.0
44	47670.0
45	53574.0
46	58006.5
47	62439.0
48	64134.0
49	65829.0
50	66775.5
51	67722.0
52	61523.5
53	55325.0
54	50757.0
55	46189.0
56	42430.0
57	38671.0
58	36813.0
59	34955.0
60	32916.5
61	30878.0
62	27724.0
63	24570.0
64	22020.5
65	19471.0
66	16994.0
67	14517.0
68	12609.0
69	10701.0
70	9764.5
71	8828.0
72	7694.5
73	6561.0
74	5476.0
75	3661.5
76	2932.0
77	2510.0
78	2088.0
79	1510.5
80	933.0
81	669.0
82	405.0
83	260.5
84	116.0
85	71.5
86	27.0
87	18.5
88	10.0
89	10.5
90	11.0
91	12.5
92	14.0
93	9.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	786059.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.946623637729438
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.55773759122278	23.818679821073864
2	9.9880279711338	6.381675409538517
3	4.033828433868205	3.8660159638787737
4	2.1248876164385115	2.715319398193325
5	1.3496271707258658	2.1558015637216426
6	0.8901745413910497	1.7062842627424963
7	0.6205918509620886	1.3878070006729406
8	0.44619704536749866	1.1403591261297894
9	0.3672604678777227	1.0559458749875428
>10	4.889290636077535	38.24554280672906
>50	0.6831209119174523	13.912808686847772
>100	0.04562214115558713	2.320349201865302
>500	0.002422414574632945	0.613815555957586
>1k	0.0012112072873164724	0.6795953276613826
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2201	0.2800044271485983	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1995	0.25379774291751633	No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	1073	0.13650374844636345	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	922	0.11729399447115293	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	907	0.11538574076500618	No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	804	0.10228239864946524	No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	793	0.10088301259829097	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12174658645216199	0.0
2	0.0	0.0	0.0	0.5260419383277846	0.0
3	0.0	0.0	0.0	0.7124147169614494	0.0
4	0.0	0.0	0.0	0.9279201688422879	0.0
5	0.0	0.0	0.0	1.5900842048752066	0.0
6	0.0	0.0	0.0	2.1747731404385675	0.0
7	0.0	0.0	0.0	2.7461043000589016	0.0
8	0.0	0.0	0.0	3.653924196529777	0.0
9	0.0	0.0	0.0	4.100328346854371	0.0
10	0.0	0.0	0.0	5.010692581600109	0.0
11	0.0	0.0	0.0	6.1550087207194375	0.0
12	0.0	0.0	0.0	7.080766202028092	0.0
13	0.0	0.0	0.0	7.43875459730122	0.0
14	0.0	0.0	0.0	7.601337813064923	0.0
15	0.0	0.0	0.0	7.831982077681192	0.0
16	0.0	0.0	0.0	8.325838136831969	0.0
17	0.0	0.0	0.0	8.831907019702083	0.0
18	0.0	0.0	0.0	9.550428148523203	0.0
19	0.0	0.0	0.0	9.88348202870268	0.0
20	0.0	0.0	0.0	10.189565923168617	0.0
21	1.2721691374311598E-4	0.0	0.0	10.57999463144624	0.0
22	1.2721691374311598E-4	0.0	0.0	10.96622518157034	0.0
23	1.2721691374311598E-4	0.0	0.0	11.379171283580495	0.0
24	1.2721691374311598E-4	0.0	0.0	11.71298846524243	0.0
25	1.2721691374311598E-4	0.0	0.0	12.016019153778533	0.0
26	1.2721691374311598E-4	0.0	0.0	12.281648069674159	0.0
27	2.5443382748623197E-4	0.0	0.0	12.57030324695729	0.0
28	2.5443382748623197E-4	0.0	0.0	12.887455012918878	0.0
29	2.5443382748623197E-4	0.0	0.0	13.232467282990209	0.0
30	2.5443382748623197E-4	0.0	0.0	13.620733303734198	0.0
31	2.5443382748623197E-4	0.0	0.0	13.976940662214922	0.0
32	2.5443382748623197E-4	0.0	0.0	14.326532741181005	0.0
33	2.5443382748623197E-4	0.0	0.0	14.67625203706083	0.0
34	2.5443382748623197E-4	0.0	0.0	15.017447799719868	0.0
35	3.816507412293479E-4	0.0	0.0	15.418308294924428	0.0
36	3.816507412293479E-4	0.0	0.0	15.7838024881084	0.0
37	3.816507412293479E-4	0.0	0.0	16.14560739079382	0.0
38	3.816507412293479E-4	0.0	0.0	16.492527914571298	0.0
39	3.816507412293479E-4	0.0	0.0	16.84988022527571	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACAA	30	2.1646229E-6	45.000004	19
CGAACTA	25	3.889677E-5	45.0	16
GGTCGTA	20	7.0319243E-4	45.0	44
CGTACTC	20	7.0319243E-4	45.0	45
TTACGCG	25	3.889677E-5	45.0	1
CCCGATA	20	7.0319243E-4	45.0	21
GCGAATG	65	0.0	41.53846	1
TTAACGG	65	0.0	41.53846	2
GCGATAC	65	0.0	41.53846	9
TCGATGG	45	1.9270374E-8	40.0	2
CTAACGC	170	0.0	39.705883	25
ACGCATT	165	0.0	39.545452	17
TTTAGCG	80	0.0	39.375	1
TTAGCGG	160	0.0	39.375	2
ATGACGC	150	0.0	39.000004	27
TACGCAT	180	0.0	38.75	16
GGTCCGT	35	6.2457693E-6	38.571426	8
AGGGCGT	70	0.0	38.571426	6
ACGAGAT	70	0.0	38.571426	15
TAACGCA	170	0.0	38.382355	26
>>END_MODULE