FastQCFastQC Report
Sat 18 Jun 2016
SRR3552593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978809
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31090.3176309167569975No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18770.19176366379957682No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT10120.10339095778645273No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTT207.032772E-445.00000434
CACGCTA207.032772E-445.00000416
CCGTACA351.2116834E-745.00000426
ACTCGTA207.032772E-445.00000432
ACGTATG207.032772E-445.00000432
TACGTCG207.032772E-445.0000041
ATCGTAC302.1651704E-644.99999640
TATTGCG556.002665E-1140.9090921
AACGCGA900.040.00000427
CGTTAGG1350.040.02
TACGAAT1650.039.54545612
TCGATAG403.457517E-739.3750041
CCCGTAC403.457517E-739.37500425
TCCCGTA403.457517E-739.37500424
CATAACG403.457517E-739.3750041
GCGTATG750.039.0000041
ATACTCG356.247339E-638.5714344
CGTTTTT24600.037.7743951
CGACGCA301.1397982E-437.49999621
CATGACG301.1397982E-437.4999961