##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552593_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	978809
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.843999186766776	31.0	31.0	34.0	30.0	34.0
2	31.98330113433775	33.0	31.0	34.0	30.0	34.0
3	32.00185225105204	33.0	31.0	34.0	30.0	34.0
4	35.66189215669247	37.0	35.0	37.0	33.0	37.0
5	35.54162047958284	37.0	35.0	37.0	33.0	37.0
6	35.48831488063555	37.0	35.0	37.0	33.0	37.0
7	35.80647909857797	37.0	35.0	37.0	35.0	37.0
8	35.623432150705604	37.0	35.0	37.0	35.0	37.0
9	37.467096236344375	39.0	37.0	39.0	35.0	39.0
10	36.97137132985087	39.0	37.0	39.0	33.0	39.0
11	36.84575131614033	39.0	37.0	39.0	33.0	39.0
12	36.76020858001919	39.0	35.0	39.0	33.0	39.0
13	36.62485939544896	39.0	35.0	39.0	32.0	39.0
14	37.788624747013976	40.0	37.0	41.0	33.0	41.0
15	37.947525002324255	40.0	37.0	41.0	33.0	41.0
16	37.9948713181019	40.0	37.0	41.0	33.0	41.0
17	37.92642180445827	40.0	37.0	41.0	33.0	41.0
18	37.909228460302266	40.0	36.0	41.0	33.0	41.0
19	37.829887138348745	40.0	36.0	41.0	33.0	41.0
20	37.732061106916674	40.0	36.0	41.0	33.0	41.0
21	37.58360517731243	39.0	35.0	41.0	33.0	41.0
22	37.64710173282019	39.0	36.0	41.0	33.0	41.0
23	33.614785928613244	39.0	35.0	41.0	0.0	41.0
24	35.23632291897602	39.0	35.0	41.0	17.0	41.0
25	36.29503304526215	39.0	34.0	41.0	29.0	41.0
26	36.89598072759854	39.0	35.0	41.0	31.0	41.0
27	30.71488921740605	38.0	31.0	40.0	0.0	41.0
28	33.259263043147335	38.0	31.0	40.0	16.0	41.0
29	35.22589187471713	38.0	32.0	40.0	25.0	41.0
30	36.219506563589015	38.0	35.0	40.0	30.0	41.0
31	36.605696310516144	38.0	35.0	40.0	31.0	41.0
32	35.07346990066499	38.0	35.0	40.0	26.0	41.0
33	35.71178646702268	39.0	35.0	40.0	26.0	41.0
34	36.126409748990866	39.0	35.0	41.0	29.0	41.0
35	36.31027708163697	39.0	35.0	41.0	30.0	41.0
36	36.30622113200839	39.0	35.0	40.0	30.0	41.0
37	36.33276665825508	39.0	35.0	40.0	30.0	41.0
38	36.27634604912705	39.0	35.0	41.0	30.0	41.0
39	36.22681646776848	39.0	35.0	41.0	30.0	41.0
40	36.115181817903185	38.0	35.0	40.0	30.0	41.0
41	36.03496085548866	38.0	35.0	40.0	29.0	41.0
42	35.973287944839086	38.0	35.0	40.0	29.0	41.0
43	35.9338859777546	38.0	35.0	40.0	29.0	41.0
44	35.85046009997865	38.0	35.0	40.0	29.0	41.0
45	35.77075915730239	38.0	35.0	40.0	29.0	41.0
46	35.6632315395547	38.0	35.0	40.0	29.0	41.0
47	35.56774712941953	38.0	34.0	40.0	28.0	41.0
48	35.498345438180486	38.0	34.0	40.0	28.0	41.0
49	35.431118839324114	37.0	34.0	40.0	28.0	41.0
50	35.309551710292816	37.0	34.0	40.0	28.0	41.0
51	34.59736986480508	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	14.0
9	38.0
10	58.0
11	50.0
12	53.0
13	50.0
14	62.0
15	84.0
16	156.0
17	277.0
18	508.0
19	893.0
20	1493.0
21	2259.0
22	3298.0
23	4612.0
24	6231.0
25	8139.0
26	10566.0
27	12768.0
28	15559.0
29	18897.0
30	24050.0
31	30223.0
32	38629.0
33	52478.0
34	78617.0
35	90855.0
36	89433.0
37	111073.0
38	156418.0
39	220872.0
40	96.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.613123704420374	19.033846235578135	23.919477650900227	15.433552409101264
2	31.216406878154984	24.86491235777358	25.705525797167784	18.213154966903655
3	31.952505545004183	24.379322217102622	26.789598379254787	16.878573858638408
4	28.724807393475132	26.37511506330653	26.167720157865325	18.732357385353016
5	24.895561851188535	30.51790492322813	25.559736373490637	19.026796852092694
6	25.340796825529804	35.23567927961431	24.672535704105705	14.750988190750187
7	81.62041828385314	6.581978710861875	7.752891524291256	4.044711480993738
8	84.20846150781205	5.584337700205045	6.694258021738664	3.5129427702442455
9	78.85593614280212	8.233679910993871	8.525769583238404	4.384614362965604
10	41.1008685044784	28.480530930957933	16.044396812861343	14.374203751702325
11	30.499413062201103	27.36488937065352	24.019497164411035	18.116200402734346
12	27.761595980421106	24.84928111613195	28.407074311740082	18.98204859170686
13	24.411810680122475	25.76999189831724	29.488286274441695	20.32991114711859
14	21.621378634646803	28.502905061150845	28.82441824707374	21.051298057128612
15	21.26247306675766	26.970736885337182	31.30692504870715	20.459864999198004
16	23.77828565123533	25.06801633413669	30.271993821062125	20.881704193565852
17	23.223938480336816	25.39055116984008	28.73134595207032	22.65416439775278
18	23.878305164746134	25.604484633876478	28.86834918763518	21.64886101374221
19	25.18428007915742	27.278559964201392	27.149525596924423	20.38763435971676
20	26.430181986475397	25.98126907292434	26.755373111608087	20.83317582899217
21	24.98485404200411	26.659235867263178	27.379703292470747	20.97620679826197
22	23.594899515635838	25.71625311986302	26.93538780293193	23.753459561569212
23	20.591862150838416	34.992833126789805	24.101842136719217	20.313462585652566
24	21.885577267883725	26.858764069394542	30.09034449008949	21.16531417263225
25	22.86533940738183	27.638487181871028	27.308800797704148	22.187372613042992
26	22.367591634322935	28.815529893983406	27.031729377232942	21.785149094460717
27	18.59606930463451	40.5381438053798	22.741821948919554	18.12396494106613
28	21.796285077068152	28.455704841291812	28.87550073609867	20.872509345541367
29	22.98395294689771	26.890026552677792	27.97961604357949	22.146404456845005
30	23.515108667778904	26.61489626678954	28.21449332811611	21.655501737315454
31	24.49384915749651	28.229920239801636	25.57638926491277	21.69984133778909
32	23.022060483710305	31.136105205407798	25.395557253764522	20.446277057117374
33	23.754583376327762	27.738098035469637	25.557080084061347	22.950238504141257
34	22.173273846072114	28.643892730859648	26.903410164802327	22.27942325826591
35	23.51102206865691	27.2361614983107	26.87306716632152	22.379749266710867
36	23.562308887637936	29.656654158267852	25.58701442262995	21.19402253146426
37	23.115847933560072	29.89633319677281	26.516000568037278	20.47181830162984
38	22.23937458687037	29.10200049243519	26.90841624872677	21.750208671967666
39	24.023072938642777	27.56727819217028	26.43416642061934	21.975482448567597
40	23.541262902159666	27.487691674269442	27.53908065822852	21.43196476534237
41	21.293122560172616	27.655650898183403	27.205512004895745	23.845714536748233
42	23.03861121015438	27.861615493931915	26.678442883136544	22.42133041277716
43	22.706779361448454	27.16055941455381	27.6613721369542	22.471289087043537
44	22.529012299641707	27.875918590858888	27.26486985714271	22.330199252356692
45	22.691454614740977	27.32341038956528	26.752512492222692	23.232622503471056
46	21.99233966994582	28.099149067897823	26.359892481577102	23.548618780579254
47	22.75959865510023	28.163921663981426	27.621323465558657	21.455156215359686
48	22.87678188492341	26.894215316777842	27.28234006838924	22.946662729909512
49	22.769099998058863	27.015995970613265	27.417912994261396	22.796991037066476
50	21.689318345049955	27.831681155363302	27.957650573298775	22.521349926287968
51	21.46762034268177	26.600593169862556	27.373062568897506	24.55872391855817
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2150.0
1	2602.5
2	3055.0
3	5134.5
4	7214.0
5	5204.5
6	3195.0
7	3159.0
8	3123.0
9	3299.0
10	3475.0
11	3263.5
12	3052.0
13	3185.5
14	3319.0
15	3201.0
16	3083.0
17	3250.0
18	3417.0
19	3487.5
20	3558.0
21	3605.5
22	3653.0
23	4434.0
24	5215.0
25	6123.0
26	8596.5
27	10162.0
28	12864.0
29	15566.0
30	17445.5
31	19325.0
32	22217.5
33	25110.0
34	28346.0
35	31582.0
36	33795.5
37	36009.0
38	37623.0
39	39237.0
40	43625.5
41	48014.0
42	52203.0
43	56392.0
44	59113.5
45	61835.0
46	64555.5
47	67276.0
48	68818.0
49	70360.0
50	71074.0
51	71788.0
52	67324.0
53	62860.0
54	58449.0
55	54038.0
56	50371.0
57	46704.0
58	43499.5
59	40295.0
60	40568.0
61	40841.0
62	37132.5
63	33424.0
64	29935.0
65	26446.0
66	22324.5
67	18203.0
68	16203.0
69	14203.0
70	12304.0
71	10405.0
72	9457.0
73	8509.0
74	7282.5
75	5170.5
76	4285.0
77	3468.0
78	2651.0
79	1966.5
80	1282.0
81	1001.0
82	720.0
83	556.0
84	392.0
85	289.0
86	186.0
87	137.0
88	88.0
89	52.5
90	17.0
91	11.5
92	6.0
93	3.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	978809.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.075699288542694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.85371246029429	20.73494622377721
2	9.097074242205954	5.108134416594436
3	3.8629319055392566	3.253635436561121
4	2.14340745911118	2.4071065309929947
5	1.3654467250067979	1.9167935822908155
6	0.9055908991894975	1.525505865845119
7	0.6891630188551189	1.3544113574712418
8	0.5347266903601934	1.2010260608088377
9	0.4418687779774655	1.1165197441942043
>10	6.2186436337866695	43.18554690362609
>50	0.836264794610036	14.900125496592445
>100	0.04706090164972561	2.0993476042672063
>500	0.0029879937555381043	0.567935395839716
>1k	0.0011204976583267892	0.6289653811385925
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3109	0.3176309167569975	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1877	0.19176366379957682	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1012	0.10339095778645273	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0216497804985446E-4	0.0	0.0	0.07294579432759608	0.0
2	1.0216497804985446E-4	0.0	0.0	0.23998553343910814	0.0
3	2.0432995609970893E-4	0.0	0.0	0.32181968085704155	0.0
4	2.0432995609970893E-4	0.0	0.0	0.4159136256409575	0.0
5	3.064949341495634E-4	0.0	0.0	0.6915547364194649	0.0
6	3.064949341495634E-4	0.0	0.0	0.9678088370662714	0.0
7	3.064949341495634E-4	0.0	0.0	1.2407936584154824	0.0
8	3.064949341495634E-4	0.0	0.0	1.708709257883816	0.0
9	3.064949341495634E-4	0.0	0.0	1.9398064382325868	0.0
10	3.064949341495634E-4	0.0	0.0	2.442151635303721	0.0
11	3.064949341495634E-4	0.0	0.0	3.085382337105605	0.0
12	3.064949341495634E-4	0.0	0.0	3.589975163693836	0.0
13	4.0865991219941785E-4	0.0	0.0	3.786540581461756	0.0
14	4.0865991219941785E-4	0.0	0.0	3.877671741882226	0.0
15	4.0865991219941785E-4	0.0	0.0	3.9989415708274034	0.0
16	4.0865991219941785E-4	0.0	0.0	4.266613813318022	0.0
17	4.0865991219941785E-4	0.0	0.0	4.533775230918391	0.0
18	4.0865991219941785E-4	0.0	0.0	4.870817493504862	0.0
19	4.0865991219941785E-4	0.0	0.0	5.041841666760318	0.0
20	4.0865991219941785E-4	0.0	0.0	5.2047948067498355	0.0
21	4.0865991219941785E-4	0.0	0.0	5.401360224517756	0.0
22	4.0865991219941785E-4	0.0	0.0	5.613556883927304	0.0
23	5.108248902492723E-4	0.0	0.0	5.824016738710004	0.0
24	6.129898682991268E-4	0.0	0.0	6.00198813047285	0.0
25	6.129898682991268E-4	0.0	0.0	6.155133432569582	0.0
26	6.129898682991268E-4	0.0	0.0	6.294384297651534	0.0
27	6.129898682991268E-4	0.0	0.0	6.450083724199512	0.0
28	7.151548463489813E-4	0.0	0.0	6.639701923460041	0.0
29	7.151548463489813E-4	0.0	0.0	6.852920232650088	0.0
30	7.151548463489813E-4	0.0	0.0	7.081871948459812	0.0
31	7.151548463489813E-4	0.0	0.0	7.293455618001061	0.0
32	7.151548463489813E-4	0.0	0.0	7.516175270149743	0.0
33	7.151548463489813E-4	0.0	0.0	7.732560693659335	0.0
34	7.151548463489813E-4	0.0	0.0	7.95364570615922	0.0
35	7.151548463489813E-4	0.0	0.0	8.186786186068987	0.0
36	7.151548463489813E-4	0.0	0.0	8.412570787559167	0.0
37	8.173198243988357E-4	0.0	0.0	8.650921681349477	0.0
38	8.173198243988357E-4	0.0	0.0	8.889374740117837	0.0
39	8.173198243988357E-4	0.0	0.0	9.136000997130186	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTT	20	7.032772E-4	45.000004	34
CACGCTA	20	7.032772E-4	45.000004	16
CCGTACA	35	1.2116834E-7	45.000004	26
ACTCGTA	20	7.032772E-4	45.000004	32
ACGTATG	20	7.032772E-4	45.000004	32
TACGTCG	20	7.032772E-4	45.000004	1
ATCGTAC	30	2.1651704E-6	44.999996	40
TATTGCG	55	6.002665E-11	40.909092	1
AACGCGA	90	0.0	40.000004	27
CGTTAGG	135	0.0	40.0	2
TACGAAT	165	0.0	39.545456	12
TCGATAG	40	3.457517E-7	39.375004	1
CCCGTAC	40	3.457517E-7	39.375004	25
TCCCGTA	40	3.457517E-7	39.375004	24
CATAACG	40	3.457517E-7	39.375004	1
GCGTATG	75	0.0	39.000004	1
ATACTCG	35	6.247339E-6	38.57143	44
CGTTTTT	2460	0.0	37.774395	1
CGACGCA	30	1.1397982E-4	37.499996	21
CATGACG	30	1.1397982E-4	37.499996	1
>>END_MODULE