##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552591_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1071668
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.910270718170178	33.0	31.0	34.0	30.0	34.0
2	32.05679837412333	33.0	31.0	34.0	30.0	34.0
3	32.08837905022824	33.0	31.0	34.0	30.0	34.0
4	35.69745294251578	37.0	35.0	37.0	33.0	37.0
5	35.525804633524565	37.0	35.0	37.0	33.0	37.0
6	35.53008581015762	37.0	35.0	37.0	33.0	37.0
7	35.85932770223614	37.0	35.0	37.0	35.0	37.0
8	35.702339717151204	37.0	35.0	37.0	35.0	37.0
9	37.45890891582094	39.0	37.0	39.0	35.0	39.0
10	37.00964757742137	39.0	37.0	39.0	33.0	39.0
11	36.89215130058936	39.0	37.0	39.0	33.0	39.0
12	36.73459970811856	39.0	35.0	39.0	33.0	39.0
13	36.57767144302153	39.0	35.0	39.0	32.0	39.0
14	37.728805003041984	40.0	36.0	41.0	33.0	41.0
15	37.87384152554709	40.0	36.0	41.0	33.0	41.0
16	37.92088687914541	40.0	36.0	41.0	33.0	41.0
17	37.90200509859397	40.0	36.0	41.0	33.0	41.0
18	37.87455443290273	40.0	36.0	41.0	33.0	41.0
19	37.8543550801181	40.0	36.0	41.0	33.0	41.0
20	37.79151938846732	40.0	36.0	41.0	33.0	41.0
21	37.70748683360892	40.0	36.0	41.0	33.0	41.0
22	37.71460377654274	40.0	36.0	41.0	33.0	41.0
23	37.614562532426085	39.0	35.0	41.0	33.0	41.0
24	37.611043718763646	39.0	35.0	41.0	33.0	41.0
25	37.45503178223107	39.0	35.0	41.0	33.0	41.0
26	37.44477767368252	39.0	35.0	41.0	33.0	41.0
27	37.23115367819138	39.0	35.0	41.0	32.0	41.0
28	37.29002265627041	39.0	35.0	41.0	32.0	41.0
29	37.25131290660914	39.0	35.0	41.0	32.0	41.0
30	37.12680326369734	39.0	35.0	41.0	32.0	41.0
31	37.11403158440861	39.0	35.0	41.0	32.0	41.0
32	37.01683263846639	39.0	35.0	41.0	32.0	41.0
33	36.9009096100658	39.0	35.0	41.0	31.0	41.0
34	36.845507190659795	39.0	35.0	41.0	31.0	41.0
35	36.72319785605244	39.0	35.0	41.0	31.0	41.0
36	36.66141006356446	39.0	35.0	41.0	31.0	41.0
37	36.61218399728274	39.0	35.0	41.0	31.0	41.0
38	36.529404629045565	39.0	35.0	41.0	31.0	41.0
39	36.4815353262391	38.0	35.0	40.0	31.0	41.0
40	36.39086638772456	38.0	35.0	40.0	31.0	41.0
41	36.304424504604036	38.0	35.0	40.0	30.0	41.0
42	36.24503017725639	38.0	35.0	40.0	30.0	41.0
43	36.169969617456154	38.0	35.0	40.0	30.0	41.0
44	36.100154152218785	38.0	35.0	40.0	30.0	41.0
45	36.0329318408313	38.0	35.0	40.0	30.0	41.0
46	35.910561853111226	38.0	35.0	40.0	30.0	41.0
47	35.849167839293514	37.0	35.0	40.0	30.0	41.0
48	35.779549263391274	37.0	35.0	40.0	30.0	41.0
49	35.70322245322245	37.0	35.0	40.0	30.0	41.0
50	35.65191551861211	37.0	34.0	40.0	30.0	41.0
51	34.93035249722862	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	33.0
10	70.0
11	58.0
12	49.0
13	57.0
14	72.0
15	81.0
16	131.0
17	202.0
18	351.0
19	589.0
20	958.0
21	1433.0
22	2086.0
23	3117.0
24	4283.0
25	5935.0
26	7978.0
27	9888.0
28	12082.0
29	14795.0
30	18820.0
31	23925.0
32	31704.0
33	44966.0
34	77857.0
35	99403.0
36	97737.0
37	138909.0
38	204620.0
39	269319.0
40	148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.36430312372862	20.232851965347475	24.6265634506209	13.776281460303005
2	29.905997006535607	23.992505141517707	27.076482642012266	19.02501520993442
3	31.760489256000923	24.807869601406406	26.538442875965316	16.89319826662735
4	28.521333099430045	27.41669994811826	25.041897304015798	19.020069648435896
5	25.07418342247786	30.203570508776973	24.146190797896363	20.57605527084881
6	25.787277403076324	34.91277149266377	23.392692512979764	15.907258591280135
7	83.02030106338904	6.018934968665668	6.777285502599685	4.183478465345611
8	84.4936118275436	4.87912301197759	6.076788706950287	4.550476453528518
9	79.55728826464913	6.492215872826286	8.337376874181183	5.6131189883434045
10	44.400597946378916	23.62588040325922	15.696559008946801	16.276962641415064
11	32.24002209639553	25.49017046324048	21.52942889029065	20.740378550073345
12	27.849949797885166	22.018386291276776	29.040617056775048	21.091046854063013
13	24.902675082208294	24.650078195859166	29.58126957229291	20.86597714963963
14	21.484638899360622	26.83461669099012	28.355703445470056	23.325040964179205
15	21.0289007416476	24.780155794518453	32.18506104502514	22.005882418808813
16	22.858571871139198	23.17051549547061	30.80272994994718	23.168182683443007
17	23.246751792532763	23.57530503850073	26.639127043076773	26.538816125889735
18	24.39402874770918	24.157201670666662	28.23924946905198	23.209520112572175
19	24.888678210042663	26.025504167335406	25.909050190917338	23.176767431704594
20	26.354057413303373	24.60706114207012	25.86211401292191	23.176767431704594
21	25.854649014433573	26.016732794111608	25.56948607217907	22.559132119275745
22	24.286999331882637	23.757171064172862	25.075209859770002	26.8806197441745
23	22.843735186643624	26.281740240447597	26.111818212356813	24.762706360551963
24	22.870795806163848	25.196049522799974	27.62711959300828	24.306035078027897
25	22.934154980833615	26.053497911666675	26.354897225633312	24.6574498818664
26	22.1956800053748	25.472814341755097	26.2905116136714	26.040994039198708
27	22.76964507664687	26.51212875629393	26.539189375814153	24.17903679124505
28	21.73098384947577	26.049112225054778	27.933464468473446	24.28643945699601
29	23.250577604258034	24.73788524057824	27.375642456432402	24.635894698731324
30	23.573905351284168	25.676142238081194	26.357136725179814	24.392815685454824
31	25.076609546986568	26.176483761762036	24.63272207437378	24.114184616877615
32	24.86255071533348	26.022144918015655	24.698040811146736	24.417263555504128
33	23.748399690949064	26.76920464173606	23.9282128420369	25.554182825277977
34	22.04078128674179	25.241026138692206	27.339903776169482	25.378288798396518
35	22.717296774747403	24.787714105487893	27.08572057764158	25.40926854212312
36	24.116424116424117	27.500587868630955	24.862364090371273	23.520623924573655
37	23.382521452539407	27.362858646521126	26.822486068446572	22.432133832492898
38	23.712287760761726	26.87483437034604	25.4176666654225	23.995211203469733
39	23.35788695752789	25.93564424803204	26.071693845481995	24.634774948958075
40	23.692785452210945	24.523919721406255	27.98086720887439	23.802427617508407
41	20.9427733215884	25.873591448097734	26.660308976287432	26.523326254026436
42	22.219661313018584	26.013746794716276	26.818660256721298	24.947931635543842
43	22.934901480682452	24.362395816614846	27.77473993811516	24.92796276458754
44	23.409395447097424	24.046906318001472	27.286342412015664	25.257355822885447
45	24.43387317714068	24.06678187647667	25.978008114453356	25.521336831929293
46	22.943672853906246	25.875457697719817	26.883978993494257	24.296890454879684
47	22.255306680800395	25.75060559800237	27.87150498102024	24.122582740176995
48	22.099008274950823	24.668834004561113	27.493216182623726	25.738941537864342
49	22.06709540641318	25.015396559382197	28.334334887297185	24.583173146907438
50	21.84986395040255	24.596983394110865	28.334428199778287	25.218724455708298
51	21.34961573920281	24.915738829562887	26.579313742688964	27.15533168854533
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1255.0
1	1735.5
2	2216.0
3	3025.0
4	3834.0
5	2930.0
6	2026.0
7	1916.0
8	1806.0
9	1913.5
10	2021.0
11	2191.5
12	2362.0
13	2352.0
14	2342.0
15	2206.0
16	2070.0
17	2043.0
18	2016.0
19	2210.5
20	2405.0
21	2694.0
22	2983.0
23	3173.5
24	3364.0
25	4370.5
26	7185.5
27	8994.0
28	9756.5
29	10519.0
30	12411.0
31	14303.0
32	15714.0
33	17125.0
34	21607.0
35	26089.0
36	27854.0
37	29619.0
38	32109.0
39	34599.0
40	37765.5
41	40932.0
42	47582.5
43	54233.0
44	63375.0
45	72517.0
46	81744.5
47	90972.0
48	88603.0
49	86234.0
50	80636.5
51	75039.0
52	68952.0
53	62865.0
54	61837.5
55	60810.0
56	59697.5
57	58585.0
58	56260.0
59	53935.0
60	51671.5
61	49408.0
62	47751.5
63	46095.0
64	42476.5
65	38858.0
66	34326.5
67	29795.0
68	26522.0
69	23249.0
70	20172.5
71	17096.0
72	15532.5
73	13969.0
74	11304.5
75	6938.5
76	5237.0
77	4043.0
78	2849.0
79	2332.5
80	1816.0
81	1290.5
82	765.0
83	516.5
84	268.0
85	158.5
86	49.0
87	45.0
88	41.0
89	60.5
90	80.0
91	42.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1071668.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.702066245026444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.37463916296018	16.884579852274598
2	10.228813201526489	4.644303898181106
3	3.8140448138590877	2.5976009407718563
4	1.8250299405272348	1.657278024360238
5	1.0816796640087107	1.2278181694111845
6	0.7069901849301417	0.9630082807720541
7	0.4873201850176804	0.7744222585966944
8	0.3853877416727398	0.6999278433180541
9	0.2970849747161952	0.6069998498768162
>10	4.262027920847855	25.481505438956276
>50	2.303601364200796	36.09013489701492
>100	0.22634631669482852	6.749321727932219
>500	0.004551754083442622	0.7470124901598815
>1k	0.002482774954605066	0.8760863283741266
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	1917	0.1788800262767947	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1795	0.16749590358207953	No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	1697	0.1583512804338657	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1657	0.15461878118969682	No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT	1149	0.10721604078875173	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1111	0.10367016650679127	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11281478965500509	0.0
2	0.0	0.0	0.0	0.607464251988489	0.0
3	0.0	0.0	0.0	0.8184437717651362	0.0
4	0.0	0.0	0.0	1.0700142208221204	0.0
5	0.0	0.0	0.0	1.809888883497501	0.0
6	0.0	0.0	0.0	2.3961712022753314	0.0
7	0.0	0.0	0.0	2.9269325947961495	0.0
8	0.0	0.0	0.0	3.856231594113102	0.0
9	0.0	0.0	0.0	4.246837640015378	0.0
10	0.0	0.0	0.0	5.080024783794982	0.0
11	0.0	0.0	0.0	6.2222628649917695	0.0
12	0.0	0.0	0.0	7.044532448482179	0.0
13	0.0	0.0	0.0	7.370659569941437	0.0
14	0.0	0.0	0.0	7.501483668449557	0.0
15	0.0	0.0	0.0	7.729352747306069	0.0
16	0.0	0.0	0.0	8.221576085130843	0.0
17	0.0	0.0	0.0	8.801046592788065	0.0
18	0.0	0.0	0.0	9.479241705453555	0.0
19	0.0	0.0	0.0	9.848665818145172	9.331248110422257E-5
20	0.0	0.0	0.0	10.188043311921229	9.331248110422257E-5
21	0.0	0.0	0.0	10.624652411007887	9.331248110422257E-5
22	0.0	0.0	0.0	11.07021950828055	9.331248110422257E-5
23	3.732499244168903E-4	0.0	0.0	11.562629471067533	9.331248110422257E-5
24	3.732499244168903E-4	0.0	0.0	11.930094021655961	9.331248110422257E-5
25	4.665624055211129E-4	0.0	0.0	12.239798146440876	9.331248110422257E-5
26	4.665624055211129E-4	0.0	0.0	12.535878648984573	9.331248110422257E-5
27	4.665624055211129E-4	0.0	0.0	12.843156649260779	9.331248110422257E-5
28	4.665624055211129E-4	0.0	0.0	13.145302463076252	9.331248110422257E-5
29	4.665624055211129E-4	0.0	0.0	13.461818398981775	9.331248110422257E-5
30	4.665624055211129E-4	0.0	0.0	13.83497501091756	9.331248110422257E-5
31	4.665624055211129E-4	0.0	0.0	14.173792629806993	9.331248110422257E-5
32	4.665624055211129E-4	0.0	0.0	14.505705125094712	9.331248110422257E-5
33	4.665624055211129E-4	0.0	0.0	14.838550745193475	9.331248110422257E-5
34	4.665624055211129E-4	0.0	0.0	15.16953011567015	9.331248110422257E-5
35	4.665624055211129E-4	0.0	0.0	15.541940227757104	9.331248110422257E-5
36	4.665624055211129E-4	0.0	0.0	15.857243101408272	9.331248110422257E-5
37	4.665624055211129E-4	0.0	0.0	16.181783910688758	9.331248110422257E-5
38	4.665624055211129E-4	0.0	0.0	16.49801990915097	9.331248110422257E-5
39	4.665624055211129E-4	0.0	0.0	16.828719342184332	9.331248110422257E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACAC	20	7.033071E-4	45.000004	39
GACGTAT	20	7.033071E-4	45.000004	30
GTTCACG	20	7.033071E-4	45.000004	1
TTCGGAT	20	7.033071E-4	45.000004	40
TATCGTG	20	7.033071E-4	45.000004	35
TGTAACG	30	2.1653632E-6	45.000004	1
GTATTAC	35	1.211829E-7	45.0	28
GTATACG	35	1.211829E-7	45.0	1
ACGTATA	25	3.890629E-5	45.0	22
ACGCATT	95	0.0	42.63158	17
ACGGGTA	145	0.0	41.89655	5
TATACGG	65	0.0	41.53846	2
AGTACGG	115	0.0	41.086956	2
TTTAGCG	50	1.0822987E-9	40.5	1
TACGCAT	100	0.0	40.5	16
GGTAACG	85	0.0	39.705883	1
AATACGG	85	0.0	39.705883	2
TAGTAAG	85	0.0	39.705883	1
CATACGA	160	0.0	39.375004	18
CGATCTA	40	3.4579352E-7	39.375004	10
>>END_MODULE