FastQCFastQC Report
Sat 18 Jun 2016
SRR3552590_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552590_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1664851
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37870.22746780342505127No Hit
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC27550.16548027421072517RNA PCR Primer, Index 14 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGT207.0342E-445.012
TCGATAG800.045.01
CGTAAGG3450.042.3913042
GCGTAAG2650.040.754721
CACAACG5400.040.41666412
AACGAGC5500.040.09090415
ACAACGA5750.038.34782413
TTATTCG659.094947E-1238.0769220
GCTACGA2800.037.7678610
TATTGCG301.1401417E-437.5000041
CGACATA552.750312E-936.8181821
TTACGCG552.750312E-936.818181
CGCGTAA552.750312E-936.8181831
ATATCGC250.002107379536.012
TACGCAC250.002107379536.036
TACGAAT2900.035.6896612
CGTTTTT22650.035.0662271
TAAGGGA8850.034.830514
TTACACG15550.034.7266934
GTCTTAC16450.034.46808631
Produced by FastQC (version 0.11.5)