##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552590_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1664851
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79585320247878	31.0	31.0	34.0	30.0	34.0
2	31.939331507744537	33.0	31.0	34.0	30.0	34.0
3	31.98766075762936	33.0	31.0	34.0	30.0	34.0
4	35.619810421473154	37.0	35.0	37.0	33.0	37.0
5	35.423448104364894	37.0	35.0	37.0	33.0	37.0
6	35.42552516711706	37.0	35.0	37.0	33.0	37.0
7	35.79349983872431	37.0	35.0	37.0	35.0	37.0
8	35.64800273417861	37.0	35.0	37.0	35.0	37.0
9	37.37406410543646	39.0	37.0	39.0	35.0	39.0
10	36.88401064119252	39.0	37.0	39.0	32.0	39.0
11	36.79926491920298	39.0	37.0	39.0	32.0	39.0
12	36.6158935544382	39.0	35.0	39.0	32.0	39.0
13	36.45349643902067	39.0	35.0	39.0	32.0	39.0
14	37.46590595795059	40.0	36.0	41.0	32.0	41.0
15	37.63493309611491	40.0	36.0	41.0	32.0	41.0
16	37.71041132209429	40.0	36.0	41.0	33.0	41.0
17	37.69086302618072	40.0	36.0	41.0	33.0	41.0
18	37.682298896417755	40.0	36.0	41.0	33.0	41.0
19	37.64791804191486	40.0	36.0	41.0	33.0	41.0
20	37.58926774828498	39.0	36.0	41.0	33.0	41.0
21	37.49741268137509	39.0	35.0	41.0	32.0	41.0
22	37.550764602958466	39.0	35.0	41.0	33.0	41.0
23	37.4642331355779	39.0	35.0	41.0	32.0	41.0
24	37.456441447312706	39.0	35.0	41.0	32.0	41.0
25	37.29673406208724	39.0	35.0	41.0	32.0	41.0
26	37.281622799878186	39.0	35.0	41.0	32.0	41.0
27	37.05794212214787	39.0	35.0	41.0	31.0	41.0
28	37.07749882722238	39.0	35.0	41.0	31.0	41.0
29	37.04922842945104	39.0	35.0	41.0	31.0	41.0
30	36.900550860107	39.0	35.0	41.0	31.0	41.0
31	36.87978924240067	39.0	35.0	41.0	31.0	41.0
32	36.80088067941215	39.0	35.0	41.0	31.0	41.0
33	36.69915385821314	39.0	35.0	41.0	31.0	41.0
34	36.589258137815335	39.0	35.0	41.0	31.0	41.0
35	36.451875873576675	39.0	35.0	41.0	30.0	41.0
36	36.40019617371164	38.0	35.0	40.0	30.0	41.0
37	36.3649840135844	38.0	35.0	40.0	30.0	41.0
38	36.269992930298265	38.0	35.0	40.0	30.0	41.0
39	36.257168359210524	38.0	35.0	40.0	30.0	41.0
40	36.11702849083792	38.0	35.0	40.0	30.0	41.0
41	36.034224684371154	38.0	35.0	40.0	30.0	41.0
42	36.00101090127585	38.0	35.0	40.0	30.0	41.0
43	35.94109683088757	38.0	35.0	40.0	30.0	41.0
44	35.868510755617166	38.0	35.0	40.0	30.0	41.0
45	35.797370455374086	38.0	35.0	40.0	30.0	41.0
46	35.71334431729927	37.0	34.0	40.0	29.0	41.0
47	35.62481867746723	37.0	34.0	40.0	29.0	41.0
48	35.545679463207215	37.0	34.0	40.0	29.0	41.0
49	35.49313542172843	37.0	34.0	40.0	29.0	41.0
50	35.40761845954983	37.0	34.0	40.0	29.0	41.0
51	34.721106573501174	36.0	33.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	14.0
9	69.0
10	105.0
11	88.0
12	81.0
13	87.0
14	110.0
15	131.0
16	223.0
17	341.0
18	588.0
19	1010.0
20	1637.0
21	2617.0
22	3670.0
23	5378.0
24	7728.0
25	10432.0
26	13852.0
27	17721.0
28	21331.0
29	26357.0
30	32961.0
31	42127.0
32	55361.0
33	77529.0
34	124661.0
35	143472.0
36	154031.0
37	214865.0
38	317068.0
39	389031.0
40	174.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.450555034654755	19.1978140986791	23.810178808794298	13.541452057871844
2	30.18246077276585	24.549884644331534	26.355031170957645	18.912623411944974
3	32.560991944624476	23.94983094583239	26.1709306118085	17.31824649773463
4	29.084284419446544	26.113027532193573	24.709658702190165	20.093029346169715
5	25.54751145898342	30.44710908063244	23.451888487318083	20.553490973066058
6	25.134441460527096	35.7260199261075	23.259078440052594	15.880460173312807
7	83.09121957460457	5.864969297552753	6.566893974295597	4.476917153547074
8	85.074400051416	4.409283473415939	6.049850707360599	4.46646576780745
9	79.92823381792124	6.2130484950304865	8.272932532701125	5.585785154347145
10	43.144161249264954	24.967279354128387	15.397894466231513	16.49066493037515
11	32.746173681608745	25.500119830543394	21.362332124616557	20.391374363231304
12	27.835103561820247	22.86835278352237	28.26355031170958	21.032993342947805
13	25.69160843823261	23.83846962881363	29.581926550784427	20.887995382169336
14	21.83762991402834	25.361488805905154	30.364879499727003	22.436001780339502
15	21.280523001758116	24.595053851666005	31.80578922678366	22.318633919792223
16	25.760383361634165	23.52961316057713	28.636256337654242	22.07374714013446
17	25.470327374641933	23.676773477025872	26.575170991277897	24.277728157054295
18	26.755967951486348	22.711882324604424	27.262139374634724	23.2700103492745
19	25.757500220740475	25.64920224092126	25.68788438124493	22.90541315709334
20	26.748219510334557	25.69010680235048	25.48179987278141	22.079873814533553
21	25.93235070285569	26.219883941565943	26.68491054154396	21.16285481403441
22	24.9424723293556	22.89850563203554	26.688454402225787	25.470567636383073
23	23.140208943623183	26.11422884089928	26.872314699633783	23.873247515843758
24	23.418371974428943	25.439694002646483	27.51969996113766	23.622234061786912
25	24.920007856558936	26.115249953299124	25.06560647169026	23.89913571845168
26	23.321786754490343	25.534116866914818	25.533336016256108	25.61076036233873
27	22.927817564454717	25.00325854986422	27.121045667149797	24.94787821853127
28	21.86994511821178	26.02533199667718	27.195707003209296	24.909015881901745
29	23.811019724888293	26.07662787841074	24.97725021638573	25.135102180315233
30	24.46146832359172	25.47489234772361	26.65283559910166	23.41080372958301
31	25.324788824945898	26.740771396359193	23.643857618489584	24.29058216020533
32	24.863065823908567	26.301332671812673	24.12077717465407	24.714824329624694
33	24.28031097077156	26.102395950148093	23.13960828927033	26.47768478981002
34	22.34716500155269	26.350586328746537	26.317490273904394	24.98475839579638
35	22.40837168010831	27.147414393239995	25.623374103748624	24.82083982290307
36	24.56357956357656	28.335809030357673	23.337283636793924	23.763327769271843
37	23.301604768234515	27.539161162170068	24.747559991855127	24.41167407774029
38	23.392183444644594	26.878141046856445	24.960491959941162	24.7691835485578
39	24.200363876406957	24.657161511750903	24.59991915192411	26.54255545991803
40	24.674880815160037	24.853815746874645	27.17624580217689	23.295057635788428
41	22.065217848323964	25.685361632962948	26.789784791551916	25.459635727161167
42	23.28742932550721	25.441375834834467	26.85381454556594	24.417380294092382
43	23.020498531099783	24.18612836824437	27.328391549754304	25.46498155090155
44	24.101195842751093	24.281512279477262	25.859251068113604	25.758040809658038
45	23.546972071374555	24.054645130405063	25.51081147802416	26.887571320196223
46	23.21042543747158	25.483061246922396	25.609438922762457	25.697074392843568
47	21.94214377142459	26.350526263311252	27.86231320400444	23.845016761259714
48	22.06041261350115	26.532464466790124	26.667851957922963	24.73927096178577
49	23.64974403114753	25.409901546745022	26.566641699467397	24.373712722640047
50	21.60763936232131	25.271570849283208	27.71509282212042	25.40569696627506
51	21.95716013024589	24.903970385337786	25.638030069958212	27.500839414458113
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1152.0
1	1635.5
2	2119.0
3	4603.0
4	7087.0
5	4919.0
6	2751.0
7	2698.5
8	2646.0
9	2680.5
10	2715.0
11	2791.5
12	2868.0
13	2872.0
14	2876.0
15	2846.0
16	2816.0
17	2889.0
18	2962.0
19	3094.0
20	3226.0
21	3445.5
22	3665.0
23	4538.0
24	5411.0
25	6631.5
26	10101.0
27	12350.0
28	13754.0
29	15158.0
30	19583.0
31	24008.0
32	26809.0
33	29610.0
34	34106.0
35	38602.0
36	40214.0
37	41826.0
38	46015.5
39	50205.0
40	56212.5
41	62220.0
42	67217.5
43	72215.0
44	81567.0
45	90919.0
46	102542.0
47	114165.0
48	125880.0
49	137595.0
50	140665.5
51	143736.0
52	134709.5
53	125683.0
54	116779.5
55	107876.0
56	102513.5
57	97151.0
58	92164.0
59	87177.0
60	83407.0
61	79637.0
62	73587.0
63	67537.0
64	62977.0
65	58417.0
66	51123.5
67	43830.0
68	38650.5
69	33471.0
70	29186.0
71	24901.0
72	22285.0
73	19669.0
74	17154.0
75	12510.5
76	10382.0
77	7970.5
78	5559.0
79	4406.5
80	3254.0
81	2546.5
82	1839.0
83	1212.5
84	586.0
85	458.5
86	331.0
87	215.5
88	100.0
89	73.5
90	47.0
91	26.5
92	6.0
93	4.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1664851.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.098019622860527
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.54058020806035	17.962803645532475
2	9.664840388622777	4.6580702667369325
3	3.499069405356258	2.5296192957607806
4	1.8210092760107086	1.755308690668684
5	1.0456416669217	1.2598946703979859
6	0.7391052739297173	1.068658403671082
7	0.5371277348837112	0.906060028664721
8	0.410262657728642	0.7909214061177382
9	0.34716099907450726	0.7529303311190407
>10	5.525047177457654	35.288647939122505
>50	1.774372273301307	27.913861164030735
>100	0.08973348998911994	3.4718051106346346
>500	0.003780905414724729	0.6578917849215314
>1k	0.0022685432488348373	0.983527262621128
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3787	0.22746780342505127	No Hit
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC	2755	0.16548027421072517	RNA PCR Primer, Index 14 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.801963058555991E-4	0.0	0.0	0.08547311441083917	0.0
2	1.801963058555991E-4	0.0	0.0	0.44322284696948855	0.0
3	1.801963058555991E-4	0.0	0.0	0.6078622050862209	0.0
4	1.801963058555991E-4	0.0	0.0	0.8040359167276832	0.0
5	1.801963058555991E-4	0.0	0.0	1.4409097270566555	0.0
6	1.801963058555991E-4	0.0	0.0	2.0406630983793743	0.0
7	1.801963058555991E-4	0.0	0.0	2.58077149246389	0.0
8	1.801963058555991E-4	0.0	0.0	3.451119649746434	0.0
9	1.801963058555991E-4	0.0	0.0	3.896925310433186	0.0
10	1.801963058555991E-4	0.0	0.0	4.739042713131686	0.0
11	1.801963058555991E-4	0.0	0.0	5.7181693737157255	0.0
12	1.801963058555991E-4	0.0	0.0	6.465743781275322	0.0
13	1.801963058555991E-4	0.0	0.0	6.779345418899349	0.0
14	1.801963058555991E-4	0.0	0.0	6.913891993938196	0.0
15	1.801963058555991E-4	0.0	0.0	7.132229851199897	0.0
16	1.801963058555991E-4	0.0	0.0	7.5484232522910455	0.0
17	1.801963058555991E-4	0.0	0.0	8.042581588382383	0.0
18	1.801963058555991E-4	0.0	0.0	8.620771468437717	0.0
19	2.402617411407988E-4	0.0	0.0	8.947467370953918	0.0
20	2.402617411407988E-4	0.0	0.0	9.289239697726703	0.0
21	2.402617411407988E-4	0.0	0.0	9.702850285100588	0.0
22	2.402617411407988E-4	0.0	0.0	10.115499825509911	0.0
23	2.402617411407988E-4	0.0	0.0	10.539141340576425	0.0
24	2.402617411407988E-4	0.0	0.0	10.894128063111955	0.0
25	2.402617411407988E-4	0.0	0.0	11.224668153486409	0.0
26	2.402617411407988E-4	0.0	0.0	11.522472581630428	0.0
27	2.402617411407988E-4	0.0	0.0	11.886409054023453	0.0
28	2.402617411407988E-4	0.0	0.0	12.209681226728398	0.0
29	2.402617411407988E-4	0.0	0.0	12.574158288038989	0.0
30	2.402617411407988E-4	0.0	0.0	12.979960368825799	0.0
31	2.402617411407988E-4	0.0	0.0	13.31440471249379	0.0
32	2.402617411407988E-4	0.0	0.0	13.647407485714938	0.0
33	3.0032717642599846E-4	0.0	0.0	13.988338896393731	0.0
34	3.0032717642599846E-4	0.0	0.0	14.352275368786756	0.0
35	3.0032717642599846E-4	0.0	0.0	14.74161952030542	0.0
36	3.0032717642599846E-4	0.0	0.0	15.063149795387094	0.0
37	3.0032717642599846E-4	0.0	0.0	15.408225721100568	0.0
38	3.603926117111982E-4	0.0	0.0	15.808802109017563	0.0
39	3.603926117111982E-4	0.0	0.0	16.459551034897416	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGT	20	7.0342E-4	45.0	12
TCGATAG	80	0.0	45.0	1
CGTAAGG	345	0.0	42.391304	2
GCGTAAG	265	0.0	40.75472	1
CACAACG	540	0.0	40.416664	12
AACGAGC	550	0.0	40.090904	15
ACAACGA	575	0.0	38.347824	13
TTATTCG	65	9.094947E-12	38.07692	20
GCTACGA	280	0.0	37.76786	10
TATTGCG	30	1.1401417E-4	37.500004	1
CGACATA	55	2.750312E-9	36.81818	21
TTACGCG	55	2.750312E-9	36.81818	1
CGCGTAA	55	2.750312E-9	36.81818	31
ATATCGC	25	0.0021073795	36.0	12
TACGCAC	25	0.0021073795	36.0	36
TACGAAT	290	0.0	35.68966	12
CGTTTTT	2265	0.0	35.066227	1
TAAGGGA	885	0.0	34.83051	4
TTACACG	1555	0.0	34.72669	34
GTCTTAC	1645	0.0	34.468086	31
>>END_MODULE