FastQCFastQC Report
Sat 18 Jun 2016
SRR3552589_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552589_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979971
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC30130.307458077841079No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16400.16735189102534667No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15040.15347392933056184No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT14660.14959626356290137No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC13150.13418764432825053No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12820.13082019774054537No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC10450.10663580861066298No Hit
TCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC9830.10030909077921694No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTA207.0327753E-445.00000417
ACGTTAA207.0327753E-445.00000432
GAATCGT207.0327753E-445.00000443
CGACACG207.0327753E-445.00000441
TCTAGCG207.0327753E-445.0000041
ATCCGAT207.0327753E-445.00000414
CTAGTCC302.1651722E-645.00000416
CGCTAAC207.0327753E-445.00000434
AACGCAT207.0327753E-445.00000423
AACGATC207.0327753E-445.00000442
TATAGCG207.0327753E-445.0000041
TTACGCC207.0327753E-445.00000445
ACGTAAC207.0327753E-445.00000445
GCTCGAT207.0327753E-445.00000427
TAAGACG207.0327753E-445.00000417
AGTAACG302.1651722E-645.0000041
TATCCGA207.0327753E-445.00000437
AGGTAAT253.890383E-545.06
GTCGATC253.890383E-545.043
CGTATCT253.890383E-545.019