##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552589_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	979971
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.759556150130972	31.0	31.0	34.0	30.0	34.0
2	31.881976099292736	33.0	31.0	34.0	30.0	34.0
3	31.944115693219494	33.0	31.0	34.0	30.0	34.0
4	35.56083292260689	37.0	35.0	37.0	33.0	37.0
5	35.39192588352104	37.0	35.0	37.0	33.0	37.0
6	35.40102921412981	37.0	35.0	37.0	33.0	37.0
7	35.77947204560135	37.0	35.0	37.0	35.0	37.0
8	35.609581303936544	37.0	35.0	37.0	35.0	37.0
9	37.34474387507385	39.0	37.0	39.0	34.0	39.0
10	36.864465377036666	39.0	37.0	39.0	32.0	39.0
11	36.778216906418656	39.0	37.0	39.0	32.0	39.0
12	36.62971047102414	39.0	35.0	39.0	32.0	39.0
13	36.41765113457439	39.0	35.0	39.0	32.0	39.0
14	37.54023129255866	39.0	36.0	41.0	32.0	41.0
15	37.70137075484887	40.0	36.0	41.0	33.0	41.0
16	37.77168406003851	40.0	36.0	41.0	33.0	41.0
17	37.75781222097389	40.0	36.0	41.0	33.0	41.0
18	37.735536051577036	39.0	36.0	41.0	33.0	41.0
19	37.713955821141646	39.0	36.0	41.0	33.0	41.0
20	37.65201011050327	39.0	36.0	41.0	33.0	41.0
21	37.57246387903316	39.0	35.0	41.0	33.0	41.0
22	37.59232058907865	39.0	35.0	41.0	33.0	41.0
23	37.485973564523846	39.0	35.0	41.0	33.0	41.0
24	37.48260101574434	39.0	35.0	41.0	33.0	41.0
25	37.31800430829075	39.0	35.0	41.0	32.0	41.0
26	37.30916833253229	39.0	35.0	41.0	32.0	41.0
27	37.0806921837483	39.0	35.0	41.0	32.0	41.0
28	37.12733438030309	39.0	35.0	41.0	32.0	41.0
29	37.108212385876726	39.0	35.0	41.0	32.0	41.0
30	36.95917328165834	39.0	35.0	41.0	31.0	41.0
31	36.94384221573904	39.0	35.0	41.0	31.0	41.0
32	36.83685843764764	39.0	35.0	41.0	31.0	41.0
33	36.71951210801136	39.0	35.0	41.0	31.0	41.0
34	36.65562756448915	39.0	35.0	41.0	31.0	41.0
35	36.517105098008	39.0	35.0	40.0	31.0	41.0
36	36.44170184627912	38.0	35.0	40.0	30.0	41.0
37	36.410751950823034	38.0	35.0	40.0	30.0	41.0
38	36.31805430977039	38.0	35.0	40.0	30.0	41.0
39	36.29661183851359	38.0	35.0	40.0	30.0	41.0
40	36.175600094288505	38.0	35.0	40.0	30.0	41.0
41	36.093009895190775	38.0	35.0	40.0	30.0	41.0
42	36.0399246508315	38.0	35.0	40.0	30.0	41.0
43	35.99590396042332	38.0	35.0	40.0	30.0	41.0
44	35.91000754103948	38.0	35.0	40.0	30.0	41.0
45	35.855923287525854	38.0	35.0	40.0	30.0	41.0
46	35.770060542607894	38.0	35.0	40.0	30.0	41.0
47	35.70634845316851	37.0	35.0	40.0	30.0	41.0
48	35.64284453315455	37.0	34.0	40.0	29.0	41.0
49	35.60061573250637	37.0	34.0	40.0	29.0	41.0
50	35.51274272401938	37.0	34.0	40.0	29.0	41.0
51	34.809083125929234	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	14.0
9	40.0
10	72.0
11	64.0
12	64.0
13	55.0
14	45.0
15	89.0
16	102.0
17	221.0
18	346.0
19	634.0
20	978.0
21	1491.0
22	2282.0
23	3199.0
24	4445.0
25	5904.0
26	7707.0
27	9785.0
28	11866.0
29	14695.0
30	18604.0
31	24310.0
32	31432.0
33	43369.0
34	72824.0
35	92156.0
36	90599.0
37	127155.0
38	186291.0
39	229017.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.2215422701284	19.06566622889861	23.536206683667167	12.17658481730582
2	30.985406711014917	24.781141482758162	26.050668846323006	18.182782959903914
3	32.998935682790616	23.883665945216745	26.279451126614973	16.83794724537767
4	27.817149691164328	26.30700296233256	26.267818129311987	19.60802921719112
5	25.051761735806465	29.89915007689003	24.475418150128935	20.57367003717457
6	25.084823938667572	35.73983311751062	22.928637684176365	16.246705259645438
7	82.25478100882577	6.2819205874459545	6.761016397424005	4.7022820063042685
8	83.50920588466393	5.305054945503489	6.134467244438866	5.051271925393711
9	78.25660147085985	7.180110431839309	8.584539746584339	5.978748350716501
10	42.23053539339429	25.405853846695464	15.809651510095707	16.553959249814536
11	31.91992416102109	26.124650627416525	21.0872566637176	20.868168547844785
12	26.94691985783253	23.519573538400625	28.958714084396377	20.574792519370472
13	24.397660747103743	25.980156555653178	28.52706865815417	21.095114039088912
14	20.447747943561595	29.04045119702522	27.895111181861505	22.616689677551683
15	19.549762186840223	26.26638951560811	32.64300678285378	21.540841514697885
16	22.344028547783555	24.62787164109958	29.839250345163276	23.188849465953584
17	21.607986358780003	24.479601947404568	26.382515400965943	27.529896292849482
18	23.259157668951428	24.561134972361426	28.40379970427696	23.77590765441018
19	24.818591570566884	27.15845672984201	25.39013909595284	22.63281260363827
20	25.52636761700091	25.441671233128325	25.83474408936591	23.19721706050485
21	24.426641196525203	26.73517889815107	25.982095388537008	22.85608451678672
22	23.14721558086923	24.961759072462346	24.51643977219734	27.374585574471084
23	22.157900590935853	27.453159328184203	25.416568449474525	24.97237163140542
24	22.41811237271307	25.306259062768184	28.29328622989864	23.982342334620103
25	23.01823217217652	26.220980008592093	26.32945260625059	24.43133521298079
26	21.056031249904333	28.299817035402068	24.93390110523679	25.710250609456807
27	22.41229587406158	27.390912588229654	26.903755315208304	23.29303622250046
28	20.902251189065797	27.162946658625614	28.794525552286753	23.140276600021835
29	22.57046381984773	26.060669142250127	26.286798282806327	25.082068755095815
30	22.681487513406008	28.070830667438116	25.25789028450842	23.989791534647452
31	24.066426455476744	28.141342958107945	23.488858343767316	24.303372242647995
32	25.023801724744914	29.217191120961743	22.357906509478344	23.401100644815
33	23.624882777143405	28.24655015301473	23.02925290646356	25.099314163378306
34	21.91595465580104	27.656838824822366	25.27462547361095	25.152581045765636
35	22.86251327845416	27.310910220812655	24.456233908962613	25.370342591770573
36	24.70603721946874	29.748023155787262	23.581208015339232	21.964731609404765
37	23.821725336770168	28.082157533233126	25.99189159679215	22.104225533204556
38	23.465694392997342	29.436075149162576	24.041935934838886	23.056294523001192
39	25.127172130603864	26.524254289157533	24.501439328306652	23.847134251931944
40	23.765601226975082	26.818956887499734	26.067710166933512	23.34773171859167
41	21.29532404530338	26.906918674124032	25.16309156087272	26.634665719699868
42	22.37198855884511	27.22090755746854	25.557797118486157	24.849306765200193
43	23.65325096354892	24.692975608461882	26.99212527717657	24.661648150812628
44	23.23925912093317	25.370444635606564	26.270165137539784	25.12013110592048
45	23.53334945625942	25.938828802076795	25.089415911287173	25.438405830376613
46	23.559880853617095	27.481323426917736	25.42840553444949	23.53039018501568
47	22.58964806101405	27.070188811709734	26.50047807537162	23.839685051904596
48	21.974017598479957	26.092608862915327	26.94140949068901	24.991964047915705
49	23.538553691894965	24.47215274737722	27.95409251906434	24.035201041663477
50	21.55410721337672	25.867602204555034	27.347646001769437	25.230644580298804
51	21.320630916629167	25.54065375403966	25.928114199297735	27.21060113003344
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1714.0
1	1985.0
2	2256.0
3	2877.5
4	3499.0
5	2557.5
6	1616.0
7	1588.0
8	1560.0
9	1633.5
10	1707.0
11	1719.5
12	1732.0
13	1766.0
14	1800.0
15	1896.5
16	1993.0
17	2026.0
18	2059.0
19	2039.5
20	2020.0
21	2356.5
22	2693.0
23	2733.5
24	2774.0
25	4161.0
26	6519.0
27	7490.0
28	8810.0
29	10130.0
30	11434.0
31	12738.0
32	13665.5
33	14593.0
34	17761.5
35	20930.0
36	21486.5
37	22043.0
38	25583.5
39	29124.0
40	35552.5
41	41981.0
42	49525.0
43	57069.0
44	66775.0
45	76481.0
46	81201.5
47	85922.0
48	89286.0
49	92650.0
50	82711.5
51	72773.0
52	67524.0
53	62275.0
54	58891.0
55	55507.0
56	51637.0
57	47767.0
58	45907.0
59	44047.0
60	42748.0
61	41449.0
62	39902.5
63	38356.0
64	34183.5
65	30011.0
66	26311.5
67	22612.0
68	20309.0
69	18006.0
70	16155.0
71	14304.0
72	12163.0
73	10022.0
74	8879.0
75	6253.5
76	4771.0
77	3787.5
78	2804.0
79	2225.0
80	1646.0
81	1373.5
82	1101.0
83	710.5
84	320.0
85	255.0
86	190.0
87	122.5
88	55.0
89	71.0
90	87.0
91	45.5
92	4.0
93	4.5
94	5.0
95	2.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	979971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.242431711550942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.62082208351023	17.111269298242284
2	10.817734110310342	5.028608926652069
3	4.2972772837230995	2.9963752143759983
4	2.172626791558001	2.019885193498915
5	1.2416515417548746	1.442950058438981
6	0.8040208315523711	1.1212439563212233
7	0.5647101010555622	0.9187665172424855
8	0.41352389373419135	0.7689040688969273
9	0.31410602489424316	0.6570529050412052
>10	3.3385760835913354	19.26278793899551
>50	1.9306341071879827	32.42096448787745
>100	0.476355769366744	14.235778058795518
>500	0.004865286409502493	0.8573602424219892
>1k	0.0030960913515015857	1.1580531331994852
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	3013	0.307458077841079	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1640	0.16735189102534667	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1504	0.15347392933056184	No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT	1466	0.14959626356290137	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1315	0.13418764432825053	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1282	0.13082019774054537	No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	1045	0.10663580861066298	No Hit
TCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	983	0.10030909077921694	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0204383599106504E-4	0.0	0.0	0.16092312935790956	0.0
2	1.0204383599106504E-4	0.0	0.0	0.7970644029262091	0.0
3	1.0204383599106504E-4	0.0	0.0	1.0680928313184779	0.0
4	1.0204383599106504E-4	0.0	0.0	1.435450640886312	0.0
5	1.0204383599106504E-4	0.0	0.0	2.418847088332206	0.0
6	1.0204383599106504E-4	0.0	0.0	3.1620323458551325	0.0
7	1.0204383599106504E-4	0.0	0.0	3.8187864742936273	0.0
8	1.0204383599106504E-4	0.0	0.0	4.971371601812707	0.0
9	1.0204383599106504E-4	0.0	0.0	5.499448453066468	0.0
10	1.0204383599106504E-4	0.0	0.0	6.663666577888529	0.0
11	1.0204383599106504E-4	0.0	0.0	8.013604484214328	0.0
12	1.0204383599106504E-4	0.0	0.0	9.098534548471333	0.0
13	1.0204383599106504E-4	0.0	0.0	9.509567119843343	0.0
14	1.0204383599106504E-4	0.0	0.0	9.667020758777555	0.0
15	1.0204383599106504E-4	0.0	0.0	9.943661598149333	0.0
16	1.0204383599106504E-4	0.0	0.0	10.561537025075232	0.0
17	1.0204383599106504E-4	0.0	0.0	11.37870406369168	0.0
18	1.0204383599106504E-4	0.0	0.0	12.316588960285559	0.0
19	1.0204383599106504E-4	0.0	0.0	12.78609264968045	0.0
20	1.0204383599106504E-4	0.0	0.0	13.2354937033851	2.0408767198213008E-4
21	8.163506879285203E-4	0.0	0.0	13.777856691677611	2.0408767198213008E-4
22	8.163506879285203E-4	0.0	0.0	14.340118227988379	2.0408767198213008E-4
23	8.163506879285203E-4	0.0	0.0	14.925033495889164	2.0408767198213008E-4
24	0.0014286137038749106	0.0	0.0	15.383822582504992	2.0408767198213008E-4
25	0.0025510958997766262	0.0	0.0	15.77648726339861	2.0408767198213008E-4
26	0.0025510958997766262	0.0	0.0	16.1443552921464	2.0408767198213008E-4
27	0.0025510958997766262	0.0	0.0	16.51916230174158	2.0408767198213008E-4
28	0.0025510958997766262	0.0	0.0	16.89458157435271	2.0408767198213008E-4
29	0.0025510958997766262	0.0	0.0	17.291634140193946	2.0408767198213008E-4
30	0.0025510958997766262	0.0	0.0	17.74348424596238	2.0408767198213008E-4
31	0.0025510958997766262	0.0	0.0	18.155945431038266	2.0408767198213008E-4
32	0.0026531397357676912	0.0	0.0	18.54463040232823	2.0408767198213008E-4
33	0.0026531397357676912	0.0	0.0	18.953009833964476	2.0408767198213008E-4
34	0.0026531397357676912	0.0	0.0	19.33281699152322	2.0408767198213008E-4
35	0.0026531397357676912	0.0	0.0	19.77864651096818	2.0408767198213008E-4
36	0.0026531397357676912	0.0	0.0	20.173862287761576	2.0408767198213008E-4
37	0.0026531397357676912	0.0	0.0	20.54968973571667	2.0408767198213008E-4
38	0.0026531397357676912	0.0	0.0	20.930007112455367	2.0408767198213008E-4
39	0.0027551835717587563	0.0	0.0	21.308079524802263	2.0408767198213008E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTA	20	7.0327753E-4	45.000004	17
ACGTTAA	20	7.0327753E-4	45.000004	32
GAATCGT	20	7.0327753E-4	45.000004	43
CGACACG	20	7.0327753E-4	45.000004	41
TCTAGCG	20	7.0327753E-4	45.000004	1
ATCCGAT	20	7.0327753E-4	45.000004	14
CTAGTCC	30	2.1651722E-6	45.000004	16
CGCTAAC	20	7.0327753E-4	45.000004	34
AACGCAT	20	7.0327753E-4	45.000004	23
AACGATC	20	7.0327753E-4	45.000004	42
TATAGCG	20	7.0327753E-4	45.000004	1
TTACGCC	20	7.0327753E-4	45.000004	45
ACGTAAC	20	7.0327753E-4	45.000004	45
GCTCGAT	20	7.0327753E-4	45.000004	27
TAAGACG	20	7.0327753E-4	45.000004	17
AGTAACG	30	2.1651722E-6	45.000004	1
TATCCGA	20	7.0327753E-4	45.000004	37
AGGTAAT	25	3.890383E-5	45.0	6
GTCGATC	25	3.890383E-5	45.0	43
CGTATCT	25	3.890383E-5	45.0	19
>>END_MODULE