##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552586_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1046418
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60565376360116	31.0	31.0	34.0	30.0	34.0
2	31.74007327855599	31.0	31.0	34.0	30.0	34.0
3	31.801123451622583	33.0	31.0	34.0	30.0	34.0
4	35.476550479827374	37.0	35.0	37.0	33.0	37.0
5	35.27284221028308	37.0	35.0	37.0	33.0	37.0
6	35.26844530579558	37.0	35.0	37.0	32.0	37.0
7	35.6782633708518	37.0	35.0	37.0	35.0	37.0
8	35.36880195103677	37.0	35.0	37.0	33.0	37.0
9	37.15901771567385	39.0	37.0	39.0	34.0	39.0
10	36.69841879631275	39.0	37.0	39.0	32.0	39.0
11	36.60475259408764	39.0	35.0	39.0	32.0	39.0
12	36.46161667708316	38.0	35.0	39.0	32.0	39.0
13	36.19019072684147	38.0	35.0	39.0	32.0	39.0
14	37.2438853307187	39.0	36.0	41.0	32.0	41.0
15	37.36199587545321	39.0	36.0	41.0	32.0	41.0
16	37.50582080965733	39.0	36.0	41.0	32.0	41.0
17	37.422559627223535	39.0	36.0	41.0	32.0	41.0
18	37.440480763901235	39.0	36.0	41.0	32.0	41.0
19	37.418624297364914	39.0	36.0	41.0	32.0	41.0
20	37.25097905425939	39.0	35.0	41.0	32.0	41.0
21	37.22816981359266	39.0	35.0	41.0	32.0	41.0
22	37.25368065151785	39.0	35.0	41.0	32.0	41.0
23	37.09310906349088	39.0	35.0	41.0	32.0	41.0
24	37.13015353329167	39.0	35.0	41.0	32.0	41.0
25	37.03855629394754	39.0	35.0	41.0	31.0	41.0
26	37.00474666911311	39.0	35.0	41.0	31.0	41.0
27	36.73915204058034	39.0	35.0	40.0	31.0	41.0
28	36.77111536689927	39.0	35.0	40.0	31.0	41.0
29	36.733068429633285	39.0	35.0	40.0	31.0	41.0
30	36.65552675890514	39.0	35.0	40.0	31.0	41.0
31	36.67188255553708	39.0	35.0	40.0	31.0	41.0
32	36.46999669348195	38.0	35.0	40.0	30.0	41.0
33	36.39286881533001	38.0	35.0	40.0	30.0	41.0
34	36.31043426240757	38.0	35.0	40.0	30.0	41.0
35	36.23440728274934	38.0	35.0	40.0	30.0	41.0
36	36.154978220940386	38.0	35.0	40.0	30.0	41.0
37	36.08845891412418	38.0	35.0	40.0	30.0	41.0
38	36.046207156222465	38.0	35.0	40.0	30.0	41.0
39	35.97761028575579	38.0	35.0	40.0	30.0	41.0
40	35.96505794051708	38.0	35.0	40.0	30.0	41.0
41	35.79136635646558	38.0	35.0	40.0	29.0	41.0
42	35.76973637685896	38.0	35.0	40.0	29.0	41.0
43	35.68498152745843	38.0	35.0	40.0	29.0	41.0
44	35.72288798548955	38.0	35.0	40.0	29.0	41.0
45	35.68003895192934	38.0	35.0	40.0	29.0	41.0
46	35.562056463096006	38.0	34.0	40.0	29.0	41.0
47	35.41688694192952	37.0	34.0	40.0	29.0	41.0
48	35.41044592122842	37.0	34.0	40.0	29.0	41.0
49	35.40588369083865	37.0	34.0	40.0	29.0	41.0
50	35.2975187735685	37.0	34.0	40.0	28.0	41.0
51	34.644154630367595	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	33.0
10	35.0
11	46.0
12	38.0
13	45.0
14	47.0
15	75.0
16	122.0
17	202.0
18	357.0
19	683.0
20	1212.0
21	2205.0
22	3577.0
23	5185.0
24	6777.0
25	8006.0
26	9627.0
27	11666.0
28	13669.0
29	17077.0
30	21685.0
31	28371.0
32	37017.0
33	50209.0
34	80937.0
35	100871.0
36	97007.0
37	132332.0
38	195318.0
39	221897.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.10423368099555	17.954106294043108	23.793837644230127	12.147822380731219
2	28.499700884350233	28.20708359374552	25.234466532494665	18.058748989409587
3	29.732477843462174	28.700863326127802	25.475957026733102	16.090701803676925
4	27.50707652200172	24.681819311212156	29.154792826576	18.656311340210127
5	26.953855916087072	29.101850312207933	23.52988958523267	20.414404186472325
6	23.47723376318068	36.93848920794558	24.290675427983846	15.293601600889891
7	77.39402418536379	5.516629109973261	12.135112354718668	4.9542343499442865
8	76.84663298987594	8.572100250569084	8.386323629754076	6.194943129800902
9	71.84987261304757	6.820314635260479	10.134382244953738	11.195430506738226
10	42.59865560416583	23.257054064436964	17.467398305457284	16.67689202593992
11	32.881028422676216	24.61597564262083	22.26290067640274	20.24009525830022
12	26.75995634631667	21.63017073483063	29.002176950320045	22.607695968532653
13	24.39713384135212	24.705232517024744	31.380289712141803	19.517343929481335
14	20.43781739228492	29.749105997794377	27.629589705070057	22.183486904850643
15	17.791934007251403	25.033399654822453	35.432207779300434	21.742458558625714
16	20.412110647943745	24.279494427657017	30.304333449921543	25.004061474477695
17	20.019246610819003	24.993167166466936	28.85663281786055	26.130953404853507
18	22.726768843808117	23.37985393982137	28.904510434644664	24.98886678172585
19	22.809527359047724	26.469919286556614	25.553650644388764	25.1669027100069
20	25.16413135095153	25.0861510409798	28.17096036192038	21.57875724614829
21	22.878237950799775	28.601094400134553	27.142881716484236	21.377785932581435
22	21.07303200059632	23.97130018787903	27.95909474034277	26.996573071181878
23	21.468667396776432	28.06134833307531	26.67394865149491	23.79603561865335
24	21.42528129294412	25.124185554912092	28.59392709223274	24.856606059911048
25	20.51436424067629	29.602032839649166	25.92205027054198	23.961552649132564
26	20.04772471421554	27.03546766206239	28.05207861485563	24.864729008866437
27	22.86103641183542	26.340047667375753	26.7558470897863	24.043068831002525
28	20.000802738485003	27.097966586966205	29.166833903851042	23.734396770697753
29	22.47849329808929	25.832793396138065	26.71399001163971	24.974723294132936
30	21.292542750602532	27.398611262420946	28.20880374764196	23.100042239334567
31	25.62608823624976	25.348092253764747	23.227715884092206	25.798103625893287
32	25.42349233289183	27.1640969478736	23.877551800523307	23.53485891871126
33	23.351949221056977	24.145704680156495	24.272804940281993	28.229541158504535
34	22.202599725922145	25.612327005078278	26.492185723104917	25.69288754589466
35	22.640952277197066	23.333983169249763	27.432823212138935	26.592241341414237
36	24.22091363107286	26.26063389582366	26.489892184576334	23.028560288527146
37	22.854824744987184	24.379263353650263	29.272145547955024	23.49376635340753
38	23.38262529887674	25.243736250714342	24.236012759719348	27.13762569068957
39	22.67076827806861	23.891121903484073	27.97753861267677	25.460571205770542
40	24.34180222435012	22.43740073278556	28.12948554019522	25.091311502669107
41	20.735881836895008	23.447131069993063	29.306357497673012	26.510629595438918
42	23.637781460181305	22.64888409794174	29.835782641353646	23.877551800523307
43	23.81868431162308	22.593170224518307	28.29442918604229	25.29371627781632
44	23.079495956682702	22.14554795502371	28.676685607472347	26.09827048082124
45	22.817841436213826	21.910555819949582	26.566821289389136	28.70478145444746
46	24.78961562205543	24.653245643710257	26.269999178148694	24.28713955608562
47	19.82859622063076	24.039532959104296	32.15216099111445	23.97970982915049
48	22.638085354036342	22.96768595341441	28.142004437997052	26.252224254552196
49	22.244934624595526	21.336502239066988	31.917933368883176	24.500629767454306
50	21.864971741694045	21.698499070161255	29.523956965572072	26.912572222572624
51	21.550183578646394	22.122325877421833	26.56930595612843	29.758184587803342
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1675.0
1	1856.5
2	2038.0
3	2853.0
4	3668.0
5	2699.5
6	1731.0
7	1766.5
8	1802.0
9	1897.5
10	1993.0
11	1982.5
12	1972.0
13	2034.0
14	2096.0
15	2092.0
16	2088.0
17	2187.0
18	2286.0
19	2325.0
20	2364.0
21	2465.0
22	2566.0
23	3144.0
24	3722.0
25	4215.5
26	5786.0
27	6863.0
28	7598.5
29	8334.0
30	10010.5
31	11687.0
32	12732.5
33	13778.0
34	17170.0
35	20562.0
36	22358.5
37	24155.0
38	26129.5
39	28104.0
40	32004.0
41	35904.0
42	42718.0
43	49532.0
44	58592.0
45	67652.0
46	95133.5
47	122615.0
48	115907.5
49	109200.0
50	107543.5
51	105887.0
52	92850.0
53	79813.0
54	71116.5
55	62420.0
56	58894.5
57	55369.0
58	49601.5
59	43834.0
60	40747.0
61	37660.0
62	36684.5
63	35709.0
64	30651.0
65	25593.0
66	22269.0
67	18945.0
68	16461.0
69	13977.0
70	12599.5
71	11222.0
72	10097.0
73	8972.0
74	7405.0
75	4797.5
76	3757.0
77	2982.0
78	2207.0
79	1623.0
80	1039.0
81	864.5
82	690.0
83	494.0
84	298.0
85	158.5
86	19.0
87	44.0
88	69.0
89	35.0
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1046418.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.484676929019745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.35072487192215	16.99133399197485
2	11.469009221875769	5.386919525434012
3	4.622356404431026	3.256636404265439
4	2.3567264657117213	2.2138783862924125
5	1.3918532418984986	1.6343611859297502
6	0.9357548318580353	1.318553994657309
7	0.6148397043438876	1.0107518273755152
8	0.4900872494456283	0.9207632576209993
9	0.3403605748654505	0.719393232608149
>10	3.3167193916137894	18.717806597215585
>50	1.6930331591532832	28.824218515920524
>100	0.4070344150489306	12.645369004614324
>500	0.004928771927938615	0.7410933214015062
>1k	0.004928771927938615	2.2198074457129846
>5k	0.0012321929819846538	2.351570230697295
>10k+	4.1073099399488455E-4	1.0475430782793644
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG	10860	1.0378261841826115	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGC	9364	0.8948622825677692	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC	9095	0.8691555382265977	No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	5920	0.5657395037164881	No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	2776	0.2652859564724613	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT	2756	0.263374674365311	No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT	2399	0.22925828875267817	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATGCCTGTT	2019	0.19294392871682253	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1945	0.18587218492036642	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTC	1839	0.17574238975246986	No Hit
GAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT	1747	0.1669504920595785	No Hit
TCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	1743	0.16656823563814843	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTA	1712	0.16360574837206546	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1698	0.16226785089706028	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1307	0.12490228570227194	No Hit
ACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	1072	0.10244472094325593	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.556410535751488E-5	0.0	0.0	0.2434973404509479	0.0
2	9.556410535751488E-5	0.0	0.0	1.406894759073334	0.0
3	1.9112821071502975E-4	0.0	0.0	1.7998543603034352	0.0
4	1.9112821071502975E-4	0.0	0.0	2.4386048405130647	0.0
5	1.9112821071502975E-4	0.0	0.0	4.708634599175473	0.0
6	1.9112821071502975E-4	0.0	0.0	5.715975833749037	0.0
7	1.9112821071502975E-4	0.0	0.0	6.893612304069693	0.0
8	1.9112821071502975E-4	0.0	0.0	8.463730555093662	0.0
9	1.9112821071502975E-4	0.0	0.0	9.038739777029829	0.0
10	1.9112821071502975E-4	0.0	0.0	11.389521204719339	0.0
11	1.9112821071502975E-4	0.0	0.0	12.89341353073055	0.0
12	1.9112821071502975E-4	0.0	0.0	15.04112123453534	0.0
13	1.9112821071502975E-4	0.0	0.0	15.567870583265961	0.0
14	1.9112821071502975E-4	0.0	0.0	15.786234564007882	0.0
15	1.9112821071502975E-4	0.0	0.0	16.30935247673492	0.0
16	1.9112821071502975E-4	0.0	0.0	17.062015370530705	0.0
17	1.9112821071502975E-4	0.0	0.0	17.959935704469917	0.0
18	1.9112821071502975E-4	0.0	0.0	19.001297760550756	9.556410535751488E-5
19	2.866923160725446E-4	0.0	0.0	19.766575116253733	1.9112821071502975E-4
20	2.866923160725446E-4	0.0	0.0	20.31281954247729	1.9112821071502975E-4
21	2.866923160725446E-4	0.0	0.0	20.92299635518502	1.9112821071502975E-4
22	3.822564214300595E-4	0.0	0.0	21.56375368160716	1.9112821071502975E-4
23	3.822564214300595E-4	0.0	0.0	22.16007369903805	1.9112821071502975E-4
24	3.822564214300595E-4	0.0	0.0	22.635218430875614	1.9112821071502975E-4
25	3.822564214300595E-4	0.0	0.0	23.076246777100547	1.9112821071502975E-4
26	3.822564214300595E-4	0.0	0.0	23.453916121473444	1.9112821071502975E-4
27	3.822564214300595E-4	0.0	0.0	23.881565492948326	1.9112821071502975E-4
28	5.733846321450892E-4	0.0	0.0	24.297938299991017	1.9112821071502975E-4
29	5.733846321450892E-4	0.0	0.0	24.721765107251596	1.9112821071502975E-4
30	5.733846321450892E-4	0.0	0.0	25.214875890896373	1.9112821071502975E-4
31	5.733846321450892E-4	0.0	0.0	25.63898939047302	1.9112821071502975E-4
32	5.733846321450892E-4	0.0	0.0	26.12091917379097	1.9112821071502975E-4
33	5.733846321450892E-4	0.0	0.0	26.520281570080027	1.9112821071502975E-4
34	5.733846321450892E-4	0.0	0.0	26.909514171201184	1.9112821071502975E-4
35	5.733846321450892E-4	0.0	0.0	27.349013491740394	1.9112821071502975E-4
36	5.733846321450892E-4	0.0	0.0	27.752389580454466	1.9112821071502975E-4
37	5.733846321450892E-4	0.0	0.0	28.17210713118467	1.9112821071502975E-4
38	5.733846321450892E-4	0.0	0.0	28.572138476211226	1.9112821071502975E-4
39	5.733846321450892E-4	0.0	0.0	28.989180231991423	1.9112821071502975E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCACG	45	3.8562575E-10	45.000004	1
CGATCGA	155	0.0	45.000004	41
ATCACGC	20	7.0329965E-4	45.0	42
CCCACGT	20	7.0329965E-4	45.0	17
CCCGTTA	20	7.0329965E-4	45.0	16
TCTACGT	20	7.0329965E-4	45.0	35
ACGTCGT	20	7.0329965E-4	45.0	37
CGTAATA	20	7.0329965E-4	45.0	22
ATGCGTA	20	7.0329965E-4	45.0	20
AATTACG	20	7.0329965E-4	45.0	1
ACGTAAG	20	7.0329965E-4	45.0	1
CGGTACG	20	7.0329965E-4	45.0	2
AGTACGA	20	7.0329965E-4	45.0	12
TAGTACG	35	1.2117926E-7	45.0	1
ATTAGCG	50	2.1827873E-11	44.999996	1
ATAGCGC	25	3.8905666E-5	44.999996	12
TATTGCG	25	3.8905666E-5	44.999996	1
CATCGAT	25	3.8905666E-5	44.999996	21
TCGCGGA	50	2.1827873E-11	44.999996	15
TCAACGT	30	2.165314E-6	44.999996	38
>>END_MODULE