##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552585_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	714817
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.664786931480364	31.0	31.0	34.0	30.0	34.0
2	31.810168196895148	33.0	31.0	34.0	30.0	34.0
3	31.84854165471722	33.0	31.0	34.0	30.0	34.0
4	35.503361000088134	37.0	35.0	37.0	33.0	37.0
5	35.32112694577773	37.0	35.0	37.0	33.0	37.0
6	35.327213818361905	37.0	35.0	37.0	32.0	37.0
7	35.726604151831864	37.0	35.0	37.0	35.0	37.0
8	35.5663155744757	37.0	35.0	37.0	35.0	37.0
9	37.2752047027421	39.0	37.0	39.0	34.0	39.0
10	36.779894714311496	39.0	37.0	39.0	32.0	39.0
11	36.71294611068287	39.0	35.0	39.0	32.0	39.0
12	36.560928181618515	39.0	35.0	39.0	32.0	39.0
13	36.32642760314878	39.0	35.0	39.0	32.0	39.0
14	37.38835813921605	39.0	36.0	41.0	32.0	41.0
15	37.55554358668023	39.0	36.0	41.0	32.0	41.0
16	37.61995867473773	39.0	36.0	41.0	33.0	41.0
17	37.62061478672164	39.0	36.0	41.0	33.0	41.0
18	37.605305973416975	39.0	36.0	41.0	33.0	41.0
19	37.57932449843806	39.0	36.0	41.0	33.0	41.0
20	37.49336823270851	39.0	35.0	41.0	32.0	41.0
21	37.40600461376828	39.0	35.0	41.0	32.0	41.0
22	37.431514639411205	39.0	35.0	41.0	32.0	41.0
23	37.32919474494871	39.0	35.0	41.0	32.0	41.0
24	37.31292204858027	39.0	35.0	41.0	32.0	41.0
25	37.15629314915566	39.0	35.0	41.0	32.0	41.0
26	37.14348847327358	39.0	35.0	41.0	32.0	41.0
27	36.92334261776091	39.0	35.0	41.0	31.0	41.0
28	36.96411808896543	39.0	35.0	41.0	31.0	41.0
29	36.94432001477301	39.0	35.0	41.0	31.0	41.0
30	36.842085456837204	39.0	35.0	40.0	31.0	41.0
31	36.80067625700004	39.0	35.0	40.0	31.0	41.0
32	36.69132519232195	39.0	35.0	40.0	31.0	41.0
33	36.58077382043236	39.0	35.0	40.0	31.0	41.0
34	36.51211708731046	39.0	35.0	40.0	30.0	41.0
35	36.389470311981945	38.0	35.0	40.0	30.0	41.0
36	36.31535204115179	38.0	35.0	40.0	30.0	41.0
37	36.24795297257899	38.0	35.0	40.0	30.0	41.0
38	36.173020507346635	38.0	35.0	40.0	30.0	41.0
39	36.147011053178645	38.0	35.0	40.0	30.0	41.0
40	36.003391077716394	38.0	35.0	40.0	30.0	41.0
41	35.918523202442024	38.0	35.0	40.0	30.0	41.0
42	35.89482203137307	38.0	35.0	40.0	30.0	41.0
43	35.885332889396864	38.0	35.0	40.0	30.0	41.0
44	35.82266650065681	38.0	35.0	40.0	30.0	41.0
45	35.797258599054025	38.0	35.0	40.0	30.0	41.0
46	35.673312190392785	38.0	34.0	40.0	29.0	41.0
47	35.5645626782799	37.0	34.0	40.0	29.0	41.0
48	35.514324645328806	37.0	34.0	40.0	29.0	41.0
49	35.48999813938393	37.0	34.0	40.0	29.0	41.0
50	35.42411274494032	37.0	34.0	40.0	29.0	41.0
51	34.72925797791603	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	27.0
10	40.0
11	32.0
12	27.0
13	31.0
14	42.0
15	46.0
16	91.0
17	174.0
18	276.0
19	468.0
20	719.0
21	1150.0
22	1670.0
23	2387.0
24	3388.0
25	4587.0
26	6160.0
27	7649.0
28	9380.0
29	11533.0
30	14614.0
31	18586.0
32	24397.0
33	33315.0
34	54356.0
35	69168.0
36	66188.0
37	92092.0
38	135761.0
39	156378.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.49714682219365	19.656639391620512	23.704248779757616	13.141965006428219
2	31.47854625729383	23.97089045168204	25.87011780637562	18.68044548464852
3	32.19075651530392	24.46220501191214	26.87541006998994	16.471628402794003
4	29.21391069322638	27.078958670540853	24.822017383470175	18.885113252762594
5	24.00362610290466	29.931856684997697	24.839644272590046	21.224872939507595
6	24.72213167845756	35.724108408166	23.967812740883332	15.5859471724931
7	81.14734260656923	6.310985888695988	7.754991837071586	4.786679667663192
8	81.79562041753344	4.888523915911345	7.473381298989812	5.842474367565405
9	76.75782752788476	7.192610136580411	9.91610440154613	6.1334579339887
10	40.6744663319423	25.446372987771692	16.714627659946533	17.16453302033947
11	31.189381338160675	26.563582007702667	22.015704718830133	20.231331935306518
12	27.515154228285	22.847526010153647	29.31533525363834	20.32198450792301
13	23.753492152536943	26.60834871022933	29.69809056024129	19.940068576992434
14	20.30155969989522	29.80007470443484	27.70317437889698	22.195191216772965
15	18.78760577882171	26.39528718539151	33.81424896162235	21.002858074164436
16	20.88142839356087	24.350148359650092	32.63632510138959	22.13209814539945
17	21.102463987286256	24.89937984127406	27.132398921682054	26.865757249757632
18	22.6252313529197	24.817121025381322	28.71084487358303	23.84680274811595
19	23.945289493674604	26.752301638041626	26.503286855237075	22.799122013046695
20	24.907913493943205	25.586688621003695	26.797767820295267	22.707630064757833
21	24.14366194424587	27.219833887554433	26.869534440283317	21.766969727916376
22	22.040746093055986	25.03004265427375	26.69718263555567	26.23202861711459
23	20.753843291359885	27.28824300485299	26.525110622718824	25.4328030810683
24	21.40785683608532	25.460782270147465	29.064921511379836	24.06643938238738
25	21.40058224692474	27.511237141813922	26.67563865996472	24.412541951296625
26	20.267564985164036	28.50827554464989	26.672700845111407	24.55145862507467
27	22.094046448251788	27.353294619462044	26.166417418723952	24.38624151356221
28	19.74631269261923	26.79189219058864	28.72357540461405	24.73821971217808
29	20.52245539767521	26.645840823595414	26.354017881499743	26.47768589722964
30	22.348657068872175	26.480483816137557	26.027500744945907	25.14335837004436
31	23.252804563965324	27.161077590488198	23.521544675070682	26.064573170475803
32	22.651951478490297	29.264133337623477	22.391045540327102	25.692869643559117
33	22.619215827267677	26.76783008798056	23.735305679635488	26.877648405116272
34	20.980614618846502	26.1882411862057	26.538400737531425	26.292743457416375
35	20.92115884205328	26.13661958235464	25.446093195880902	27.49612837971117
36	24.092320132285604	27.7960652866398	24.60657762756062	23.505036953513976
37	21.529286516688888	27.366584734274646	26.844912753893652	24.259215995142814
38	21.289364970335065	29.376749573667105	24.119879633528583	25.21400582246925
39	23.584357954553404	26.497691017421243	26.28994553850846	23.628005489516898
40	23.387384463436096	26.26742229129973	28.201203944506076	22.143989300758097
41	19.861866743516174	27.801940916346425	26.201111613182114	26.13508072695529
42	20.51112382609815	26.965922746661036	26.471390579686826	26.05156284755399
43	21.954150502855978	25.350824057066358	26.431660131194416	26.26336530888325
44	21.866295849147406	24.97968011393126	26.822809194521113	26.331214842400225
45	22.88753625053685	24.55649487910892	25.372927616438893	27.183041253915334
46	23.2368564261902	26.016868653095827	26.769648735270707	23.976626185443266
47	21.198432605827787	26.27050000209844	28.27772702663759	24.253340365436188
48	20.777066018295592	25.597180816908384	27.624832649475323	26.0009205153207
49	21.28628725953636	24.86762346166921	28.760507934198543	25.08558134459589
50	20.361155372633835	23.69501564736149	29.767758741048407	26.17607023895626
51	20.36269422803319	24.53970736566142	26.452644523003787	28.6449538833016
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1480.0
1	1628.5
2	1777.0
3	2263.0
4	2749.0
5	2005.5
6	1262.0
7	1259.5
8	1257.0
9	1300.0
10	1343.0
11	1461.5
12	1580.0
13	1545.5
14	1511.0
15	1499.5
16	1488.0
17	1536.5
18	1585.0
19	1748.0
20	1911.0
21	1968.0
22	2025.0
23	2298.0
24	2571.0
25	3269.0
26	4305.0
27	4643.0
28	5767.5
29	6892.0
30	8740.5
31	10589.0
32	12033.5
33	13478.0
34	14333.5
35	15189.0
36	16068.0
37	16947.0
38	18808.0
39	20669.0
40	23740.0
41	26811.0
42	31637.0
43	36463.0
44	42337.0
45	48211.0
46	53891.0
47	59571.0
48	66372.5
49	73174.0
50	72261.5
51	71349.0
52	63285.5
53	55222.0
54	49689.5
55	44157.0
56	40534.0
57	36911.0
58	33857.5
59	30804.0
60	29250.5
61	27697.0
62	26558.5
63	25420.0
64	21907.0
65	18394.0
66	16232.0
67	14070.0
68	12043.0
69	10016.0
70	8161.5
71	6307.0
72	6079.5
73	5852.0
74	5052.0
75	3202.5
76	2153.0
77	1837.0
78	1521.0
79	1206.0
80	891.0
81	697.0
82	503.0
83	288.5
84	74.0
85	72.5
86	71.0
87	38.5
88	6.0
89	4.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	714817.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.663163504921187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.59285503489565	18.886254715544894
2	11.000268671049678	5.646033870064201
3	4.1242767703962135	3.1752596729467895
4	2.080378935896863	2.1355641913646064
5	1.2169143167940208	1.561493554168221
6	0.7672127777103436	1.1813464174467183
7	0.5252288729982802	0.9435324111682245
8	0.4110058822227665	0.843816892557379
9	0.34081154085584014	0.78716720676128
>10	4.034715320703132	26.948046353578786
>50	1.7604110367122288	30.59338287887656
>100	0.13821181426799087	5.195225401900343
>500	0.0038545127485097056	0.6637464289175798
>1k	0.0038545127485097056	1.4391300047044142
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	2932	0.4101749119005284	No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT	1419	0.1985123465166609	No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	1359	0.19011858979291202	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1190	0.16647617502101938	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1146	0.16032075342360352	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1084	0.15164720480906302	No Hit
TCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	1054	0.14745032644718858	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCC	762	0.10660071039161072	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	730	0.10212404013894465	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2106832937660968	0.0
2	0.0	0.0	0.0	1.1156701645316214	0.0
3	0.0	0.0	0.0	1.491710465755571	0.0
4	0.0	0.0	0.0	1.961900738230904	0.0
5	0.0	0.0	0.0	3.2773423127877486	0.0
6	0.0	0.0	0.0	4.201075240236312	0.0
7	0.0	0.0	0.0	5.0121919316412455	0.0
8	0.0	0.0	0.0	6.4028975248210385	0.0
9	0.0	0.0	0.0	7.008367176494124	0.0
10	1.398959453958146E-4	0.0	0.0	8.276384025561788	0.0
11	1.398959453958146E-4	0.0	0.0	9.806425980355812	0.0
12	1.398959453958146E-4	0.0	0.0	10.946717831277097	0.0
13	1.398959453958146E-4	0.0	0.0	11.405996220011556	0.0
14	1.398959453958146E-4	0.0	0.0	11.582125215264886	0.0
15	1.398959453958146E-4	0.0	0.0	11.892414422152802	0.0
16	1.398959453958146E-4	0.0	0.0	12.604065096381312	0.0
17	1.398959453958146E-4	0.0	0.0	13.452254213316136	0.0
18	1.398959453958146E-4	0.0	0.0	14.510567040235474	0.0
19	1.398959453958146E-4	0.0	0.0	15.018109530131488	0.0
20	1.398959453958146E-4	0.0	0.0	15.513341176832672	0.0
21	1.398959453958146E-4	0.0	0.0	16.106499985310926	0.0
22	1.398959453958146E-4	0.0	0.0	16.685109615468015	0.0
23	1.398959453958146E-4	0.0	0.0	17.28624249283383	0.0
24	1.398959453958146E-4	0.0	0.0	17.791826439494304	0.0
25	1.398959453958146E-4	0.0	0.0	18.215291466207436	0.0
26	2.797918907916292E-4	0.0	0.0	18.594969062011675	0.0
27	2.797918907916292E-4	0.0	0.0	18.986118125338372	0.0
28	2.797918907916292E-4	0.0	0.0	19.39027751158688	0.0
29	4.196878361874438E-4	0.0	0.0	19.799053464033452	0.0
30	4.196878361874438E-4	0.0	0.0	20.25707278925935	0.0
31	4.196878361874438E-4	0.0	0.0	20.683195838935	0.0
32	4.196878361874438E-4	0.0	0.0	21.085816369784155	0.0
33	4.196878361874438E-4	0.0	0.0	21.50004826410116	0.0
34	4.196878361874438E-4	0.0	0.0	21.905326817912837	0.0
35	4.196878361874438E-4	0.0	0.0	22.368242501227588	0.0
36	4.196878361874438E-4	0.0	0.0	22.767085841551054	0.0
37	4.196878361874438E-4	0.0	0.0	23.157675321096168	0.0
38	5.595837815832584E-4	0.0	0.0	23.54392802633401	0.0
39	5.595837815832584E-4	0.0	0.0	23.919548639721775	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGAT	25	3.8893195E-5	45.000004	18
CGTGATA	25	3.8893195E-5	45.000004	39
TCTAGTA	25	3.8893195E-5	45.000004	19
CGTTACT	25	3.8893195E-5	45.000004	19
TGCGAAC	30	2.1643446E-6	45.000004	33
CGGTATG	25	3.8893195E-5	45.000004	6
GTAAGCG	25	3.8893195E-5	45.000004	1
TATCGCG	60	0.0	45.000004	1
CAATACG	30	2.1643446E-6	45.000004	1
ACTATCG	20	7.0314953E-4	45.0	44
GATACGA	20	7.0314953E-4	45.0	11
CGAGTTA	20	7.0314953E-4	45.0	38
CGTTCTA	20	7.0314953E-4	45.0	27
TCGATAT	20	7.0314953E-4	45.0	16
CTACGAT	20	7.0314953E-4	45.0	35
GTCGTAC	20	7.0314953E-4	45.0	18
TATTACG	20	7.0314953E-4	45.0	1
CGAATCG	20	7.0314953E-4	45.0	43
TTTATCG	20	7.0314953E-4	45.0	1
CCGCGAA	20	7.0314953E-4	45.0	40
>>END_MODULE