FastQCFastQC Report
Sat 18 Jun 2016
SRR3552584_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552584_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488496
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC58640.39395470327095267TruSeq Adapter, Index 27 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC47010.31582214530640323TruSeq Adapter, Index 27 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT32840.22062538293687053TruSeq Adapter, Index 27 (100% over 25bp)
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC21640.14538164697788908TruSeq Adapter, Index 27 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC15330.10298986359385581TruSeq Adapter, Index 27 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATA351.2122473E-745.0000049
TCGATAG600.045.0000041
AACGTAG253.8913622E-545.01
CTATACG207.033958E-445.01
ATTACGC253.8913622E-545.012
ATTCACG207.033958E-445.02
ACTACGC253.8913622E-545.016
ACTCGAT253.8913622E-545.025
CAATACG406.8175723E-945.021
GCGTAAG2050.042.8048821
CGTAAGG3050.042.7868842
CCGTAGT700.041.78571727
CGTAGTG700.041.78571728
GCTACGA2050.040.6097610
CGCATGG1900.040.2631572
TATACGG1900.040.2631572
TACCGGT451.9290383E-840.040
CACAACG3100.039.91935312
CTAACGG1650.039.5454522
CTACGGT403.4591358E-739.37515
Produced by FastQC (version 0.11.5)