##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552584_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1488496
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.666734072513464	31.0	31.0	34.0	30.0	34.0
2	31.81279761584848	33.0	31.0	34.0	30.0	34.0
3	31.867652986638863	33.0	31.0	34.0	30.0	34.0
4	35.512609372144766	37.0	35.0	37.0	33.0	37.0
5	35.330234008018834	37.0	35.0	37.0	33.0	37.0
6	35.331273312121766	37.0	35.0	37.0	32.0	37.0
7	35.74024182799282	37.0	35.0	37.0	35.0	37.0
8	35.58030589265944	37.0	35.0	37.0	35.0	37.0
9	37.297387429996455	39.0	37.0	39.0	34.0	39.0
10	36.7926060936677	39.0	37.0	39.0	32.0	39.0
11	36.709990487041956	39.0	37.0	39.0	32.0	39.0
12	36.57492932463372	39.0	35.0	39.0	32.0	39.0
13	36.39565171824446	39.0	35.0	39.0	32.0	39.0
14	37.48827675720996	39.0	36.0	41.0	32.0	41.0
15	37.625768561017296	39.0	36.0	41.0	33.0	41.0
16	37.667833168513724	39.0	36.0	41.0	33.0	41.0
17	37.65101283443153	39.0	36.0	41.0	33.0	41.0
18	37.65049150283239	39.0	36.0	41.0	33.0	41.0
19	37.62687303157012	39.0	36.0	41.0	33.0	41.0
20	37.561820119100084	39.0	36.0	41.0	32.0	41.0
21	37.4803707903817	39.0	36.0	41.0	32.0	41.0
22	37.51882101127581	39.0	36.0	41.0	32.0	41.0
23	37.424867786006814	39.0	35.0	41.0	32.0	41.0
24	37.41603067794606	39.0	35.0	41.0	32.0	41.0
25	37.24883640936892	39.0	35.0	41.0	32.0	41.0
26	37.24681490578409	39.0	35.0	41.0	32.0	41.0
27	37.00872491427589	39.0	35.0	41.0	31.0	41.0
28	37.05498637550924	39.0	35.0	41.0	31.0	41.0
29	37.05466054325977	39.0	35.0	41.0	31.0	41.0
30	36.914156302737794	39.0	35.0	41.0	31.0	41.0
31	36.93951814448947	39.0	35.0	41.0	31.0	41.0
32	36.848757403446164	39.0	35.0	41.0	31.0	41.0
33	36.76461878298632	39.0	35.0	41.0	31.0	41.0
34	36.713655260074596	39.0	35.0	40.0	31.0	41.0
35	36.60816690135546	39.0	35.0	40.0	31.0	41.0
36	36.56665520095452	39.0	35.0	40.0	30.0	41.0
37	36.51306889638938	39.0	35.0	40.0	30.0	41.0
38	36.43257959712354	38.0	35.0	40.0	30.0	41.0
39	36.41703571927637	38.0	35.0	40.0	30.0	41.0
40	36.315970617321106	38.0	35.0	40.0	30.0	41.0
41	36.25226201481227	38.0	35.0	40.0	30.0	41.0
42	36.21995087658952	38.0	35.0	40.0	30.0	41.0
43	36.135130359772546	38.0	35.0	40.0	30.0	41.0
44	36.055130144790446	38.0	35.0	40.0	30.0	41.0
45	35.98480882716514	38.0	35.0	40.0	30.0	41.0
46	35.872269727295205	38.0	35.0	40.0	30.0	41.0
47	35.78845492362761	38.0	35.0	40.0	30.0	41.0
48	35.73925156668207	37.0	34.0	40.0	30.0	41.0
49	35.6804539615827	37.0	34.0	40.0	29.0	41.0
50	35.5909327267255	37.0	34.0	40.0	29.0	41.0
51	34.88516327890703	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	45.0
10	77.0
11	86.0
12	65.0
13	66.0
14	94.0
15	111.0
16	202.0
17	314.0
18	502.0
19	867.0
20	1364.0
21	2102.0
22	3213.0
23	4640.0
24	6346.0
25	8644.0
26	11355.0
27	14346.0
28	18404.0
29	23342.0
30	29500.0
31	38125.0
32	49850.0
33	67289.0
34	108088.0
35	135673.0
36	139132.0
37	192347.0
38	287490.0
39	344640.0
40	165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.44627059797272	19.57277681633004	23.567144285238253	13.413808300458987
2	30.339819522524753	23.951827885328548	26.36433016951339	19.344022422633316
3	32.04959905837839	24.758145134417557	26.120191119089338	17.072064688114715
4	29.3606432264514	26.13671786823747	25.125495802474447	19.377143102836687
5	24.865972095323063	30.30958766432694	24.155926519117283	20.668513721232706
6	23.955993163569133	36.054312540980966	23.38407358837377	16.605620707076135
7	81.23723543764982	5.783556018961422	7.837844374455827	5.141364168932936
8	82.37637185454311	4.699979039244983	7.265051434468081	5.658597671743827
9	77.14269974524622	6.687488579075791	9.513831411035031	6.655980264642968
10	42.026179445561155	24.464761746084637	16.92177876191807	16.587280046436135
11	31.643081338478574	25.779511668153628	22.039629263363825	20.537777730003977
12	26.51488482333846	22.7474578366351	29.393495179026345	21.344162161000096
13	24.378164267824705	24.89365104105083	30.45396158269824	20.274223108426224
14	21.1523578162118	27.762251292579894	28.04750567015296	23.037885221055348
15	19.920308821790584	26.56265115929099	32.44711440272597	21.069925616192453
16	22.292770689340113	24.727241457148693	30.496017456546742	22.48397039696445
17	22.500497146112586	24.510176715288452	27.036821059646783	25.952505078952175
18	23.47933753265041	24.260797476110113	28.49661671915813	23.76324827208135
19	24.27013576119788	26.725365738302287	25.81599144371231	23.18850705678752
20	26.1963082198407	25.421768012812933	25.71595758403113	22.665966183315238
21	24.929257451817136	26.737189753952983	25.967486644236864	22.366066149993014
22	23.038288312497983	24.526031645365524	25.683508722898818	26.75217131923767
23	22.147187496640903	27.04851071148327	26.252741021809932	24.55156077006589
24	22.815849018069244	24.692508411174767	27.977972396298007	24.513670174457978
25	22.381719534348765	26.055629306360245	26.03332491320098	25.52932624609001
26	21.77358891122314	25.857509862303964	26.27249250249917	26.09640872397373
27	23.076044544291687	26.107157829110726	26.182535928883922	24.634261697713665
28	21.082555814728423	26.243201191000846	27.45966398297342	25.21457901129731
29	22.425051864432284	25.016728294869452	25.42620201868194	27.13201782201632
30	22.752765207296495	25.887808902408878	26.523349743633844	24.83607614666079
31	23.36492674484849	26.912803259128676	23.807185239328827	25.915084756694007
32	22.993142070922595	26.25294256753125	24.486259956358634	26.26765540518752
33	22.226193419397834	26.391941933334053	24.339265943610194	27.042598703657923
34	20.55752921069321	25.02337930367297	27.488619384936204	26.930472100697617
35	21.322999860261632	24.477727854156143	26.930740828326044	27.268531457256184
36	22.67792456278015	26.014178069675697	25.86416087110748	25.44373649643667
37	21.418062258816953	26.852070815104646	27.27430910126732	24.455557824811084
38	21.3549784480442	26.655966828261548	26.323819479528332	25.665235244165924
39	22.326831916242973	23.55693263535811	28.14518816308542	25.971047285313496
40	22.313328352914617	23.44702303533231	30.558429448248432	23.68121916350464
41	19.621013425632317	24.727913276219756	28.099370102438968	27.55170319570896
42	21.59757231460481	24.493246874697682	26.984083262568394	26.925097548129116
43	22.088470509830056	23.80819296793542	27.15479248852533	26.948544033709194
44	21.99387838462448	23.555656179123087	27.312199696875233	27.1382657393772
45	22.503050058582623	22.98803624598252	26.979380529070955	27.5295331663639
46	22.906007137405812	24.46368683557094	27.097687867485032	25.532618159538217
47	20.670193268910364	24.68149057840935	29.585836979071495	25.0624791736088
48	21.116079586374433	23.89935881587858	27.885395728305618	27.09916586944137
49	21.765930173813032	23.236273392740053	29.111868624437015	25.8859278090099
50	21.3571282690716	22.367880061484883	29.703674044135823	26.571317625307696
51	20.20334619642915	23.753641259365157	26.90131515301351	29.141697391192185
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1517.0
1	1902.0
2	2287.0
3	2929.0
4	3571.0
5	2815.5
6	2060.0
7	1979.0
8	1898.0
9	2010.0
10	2122.0
11	2212.0
12	2302.0
13	2254.0
14	2206.0
15	2349.5
16	2493.0
17	2583.0
18	2673.0
19	2862.0
20	3051.0
21	3162.5
22	3274.0
23	4230.5
24	5187.0
25	5919.5
26	8666.5
27	10681.0
28	12292.5
29	13904.0
30	17034.5
31	20165.0
32	21953.5
33	23742.0
34	26804.0
35	29866.0
36	34129.0
37	38392.0
38	41327.5
39	44263.0
40	49255.5
41	54248.0
42	63646.0
43	73044.0
44	84346.0
45	95648.0
46	108754.5
47	121861.0
48	133818.0
49	145775.0
50	143634.5
51	141494.0
52	127658.0
53	113822.0
54	102057.0
55	90292.0
56	85898.5
57	81505.0
58	75271.0
59	69037.0
60	67325.0
61	65613.0
62	61971.0
63	58329.0
64	51088.5
65	43848.0
66	38824.0
67	33800.0
68	30016.5
69	26233.0
70	21671.5
71	17110.0
72	15256.0
73	13402.0
74	11064.0
75	7596.0
76	6466.0
77	4707.5
78	2949.0
79	2311.0
80	1673.0
81	1259.0
82	845.0
83	612.5
84	380.0
85	220.0
86	60.0
87	41.0
88	22.0
89	12.5
90	3.0
91	2.0
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1488496.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.30607153278497
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.98879176730416	16.280932102530333
2	10.916096935960937	4.869904782247189
3	4.577128023325579	3.062932353090451
4	2.374408076068561	2.118548655712306
5	1.3620293029445445	1.5190761530615127
6	0.8538667620517674	1.1427847844276522
7	0.6108989276271267	0.9538728625266623
8	0.4264197111255581	0.7609398863484962
9	0.32446684545553695	0.6513822598273463
>10	3.135382842517191	16.51072664817027
>50	1.6566839423640518	27.471087625680056
>100	0.7647158719295438	22.12075101365211
>500	0.005770294506089561	0.8672519637028053
>1k	0.00303699710846819	1.2725611731102326
>5k	3.03699710846819E-4	0.39724773591261137
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	5864	0.39395470327095267	TruSeq Adapter, Index 27 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	4701	0.31582214530640323	TruSeq Adapter, Index 27 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	3284	0.22062538293687053	TruSeq Adapter, Index 27 (100% over 25bp)
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	2164	0.14538164697788908	TruSeq Adapter, Index 27 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	1533	0.10298986359385581	TruSeq Adapter, Index 27 (100% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.23661467682815407	0.0
2	0.0	0.0	0.0	1.257242209586052	0.0
3	0.0	0.0	0.0	1.6272129720200794	0.0
4	0.0	0.0	0.0	2.08095957261558	0.0
5	6.718190710623341E-5	0.0	0.0	3.398463952875923	0.0
6	6.718190710623341E-5	0.0	0.0	4.28835549440509	0.0
7	6.718190710623341E-5	0.0	0.0	5.189332050606787	0.0
8	6.718190710623341E-5	0.0	0.0	6.602234738957982	0.0
9	6.718190710623341E-5	0.0	0.0	7.144527093119498	0.0
10	6.718190710623341E-5	0.0	0.0	8.322830561855726	0.0
11	6.718190710623341E-5	0.0	0.0	9.627771925487203	0.0
12	6.718190710623341E-5	0.0	0.0	10.722769829411702	0.0
13	6.718190710623341E-5	0.0	0.0	11.148703120465221	0.0
14	6.718190710623341E-5	0.0	0.0	11.294487858885748	0.0
15	6.718190710623341E-5	0.0	0.0	11.584310606142038	0.0
16	6.718190710623341E-5	0.0	0.0	12.222404359837043	0.0
17	6.718190710623341E-5	0.0	0.0	12.975177628962388	0.0
18	1.3436381421246682E-4	0.0	0.0	13.895636938224893	0.0
19	1.3436381421246682E-4	0.0	0.0	14.37014274811622	6.718190710623341E-5
20	1.3436381421246682E-4	0.0	0.0	14.820261525727982	6.718190710623341E-5
21	2.015457213187002E-4	0.0	0.0	15.367659704829572	6.718190710623341E-5
22	2.015457213187002E-4	0.0	0.0	15.952679751910653	6.718190710623341E-5
23	2.015457213187002E-4	0.0	0.0	16.52742096720448	6.718190710623341E-5
24	2.6872762842493364E-4	0.0	0.0	17.00414378003031	6.718190710623341E-5
25	2.6872762842493364E-4	0.0	0.0	17.417715600176287	6.718190710623341E-5
26	2.6872762842493364E-4	0.0	0.0	17.78466317679053	6.718190710623341E-5
27	3.35909535531167E-4	0.0	0.0	18.165987681525515	6.718190710623341E-5
28	3.35909535531167E-4	0.0	0.0	18.544759273790458	6.718190710623341E-5
29	3.35909535531167E-4	0.0	0.0	18.949328718384194	6.718190710623341E-5
30	3.35909535531167E-4	0.0	0.0	19.40032086078834	6.718190710623341E-5
31	4.030914426374004E-4	0.0	0.0	19.81429577237695	6.718190710623341E-5
32	4.7027334974363385E-4	0.0	0.0	20.224038223817868	6.718190710623341E-5
33	4.7027334974363385E-4	0.0	0.0	20.61940374713805	6.718190710623341E-5
34	4.7027334974363385E-4	0.0	0.0	21.01302254087347	6.718190710623341E-5
35	5.374552568498673E-4	0.0	0.0	21.43935892336963	6.718190710623341E-5
36	5.374552568498673E-4	0.0	0.0	21.841308273586225	6.718190710623341E-5
37	5.374552568498673E-4	0.0	0.0	22.241913985660695	6.718190710623341E-5
38	5.374552568498673E-4	0.0	0.0	22.631501864969742	6.718190710623341E-5
39	5.374552568498673E-4	0.0	0.0	23.01591673743161	6.718190710623341E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATA	35	1.2122473E-7	45.000004	9
TCGATAG	60	0.0	45.000004	1
AACGTAG	25	3.8913622E-5	45.0	1
CTATACG	20	7.033958E-4	45.0	1
ATTACGC	25	3.8913622E-5	45.0	12
ATTCACG	20	7.033958E-4	45.0	2
ACTACGC	25	3.8913622E-5	45.0	16
ACTCGAT	25	3.8913622E-5	45.0	25
CAATACG	40	6.8175723E-9	45.0	21
GCGTAAG	205	0.0	42.804882	1
CGTAAGG	305	0.0	42.786884	2
CCGTAGT	70	0.0	41.785717	27
CGTAGTG	70	0.0	41.785717	28
GCTACGA	205	0.0	40.60976	10
CGCATGG	190	0.0	40.263157	2
TATACGG	190	0.0	40.263157	2
TACCGGT	45	1.9290383E-8	40.0	40
CACAACG	310	0.0	39.919353	12
CTAACGG	165	0.0	39.545452	2
CTACGGT	40	3.4591358E-7	39.375	15
>>END_MODULE