Basic Statistics
Measure | Value |
---|---|
Filename | SRR3552580_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 997990 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC | 3175 | 0.31813946031523366 | RNA PCR Primer, Index 27 (95% over 23bp) |
GAATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG | 1641 | 0.1644305053156845 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC | 1423 | 0.14258659906411889 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC | 1358 | 0.13607350775057866 | No Hit |
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT | 1334 | 0.13366867403480995 | RNA PCR Primer, Index 38 (95% over 24bp) |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1333 | 0.13356847262998628 | No Hit |
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC | 1206 | 0.12084289421737693 | RNA PCR Primer, Index 38 (95% over 23bp) |
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC | 1046 | 0.10481066944558563 | RNA PCR Primer, Index 27 (95% over 23bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATTA | 20 | 7.032838E-4 | 45.000004 | 17 |
CGTATGG | 140 | 0.0 | 45.000004 | 2 |
GTCGACA | 20 | 7.032838E-4 | 45.000004 | 18 |
CACGGTA | 20 | 7.032838E-4 | 45.000004 | 39 |
CTCCGAT | 20 | 7.032838E-4 | 45.000004 | 23 |
TTTACGT | 30 | 2.165214E-6 | 45.000004 | 17 |
ACGTTAG | 35 | 1.2117198E-7 | 45.000004 | 1 |
ATAATCG | 20 | 7.032838E-4 | 45.000004 | 40 |
GACGTAC | 20 | 7.032838E-4 | 45.000004 | 27 |
ATTACCG | 20 | 7.032838E-4 | 45.000004 | 20 |
CGCGATT | 20 | 7.032838E-4 | 45.000004 | 13 |
CGTTACG | 20 | 7.032838E-4 | 45.000004 | 45 |
ACGATTG | 20 | 7.032838E-4 | 45.000004 | 34 |
CGTAGTA | 20 | 7.032838E-4 | 45.000004 | 32 |
CGCTAAA | 20 | 7.032838E-4 | 45.000004 | 26 |
TATTCGA | 20 | 7.032838E-4 | 45.000004 | 14 |
TCCGTTA | 30 | 2.165214E-6 | 45.000004 | 27 |
ACGTCGA | 20 | 7.032838E-4 | 45.000004 | 21 |
CTCGATT | 20 | 7.032838E-4 | 45.000004 | 14 |
CGGTACC | 20 | 7.032838E-4 | 45.000004 | 23 |