FastQCFastQC Report
Sat 18 Jun 2016
SRR3552580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997990
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC31750.31813946031523366RNA PCR Primer, Index 27 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG16410.1644305053156845No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC14230.14258659906411889No Hit
GAATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC13580.13607350775057866No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT13340.13366867403480995RNA PCR Primer, Index 38 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13330.13356847262998628No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC12060.12084289421737693RNA PCR Primer, Index 38 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC10460.10481066944558563RNA PCR Primer, Index 27 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA207.032838E-445.00000417
CGTATGG1400.045.0000042
GTCGACA207.032838E-445.00000418
CACGGTA207.032838E-445.00000439
CTCCGAT207.032838E-445.00000423
TTTACGT302.165214E-645.00000417
ACGTTAG351.2117198E-745.0000041
ATAATCG207.032838E-445.00000440
GACGTAC207.032838E-445.00000427
ATTACCG207.032838E-445.00000420
CGCGATT207.032838E-445.00000413
CGTTACG207.032838E-445.00000445
ACGATTG207.032838E-445.00000434
CGTAGTA207.032838E-445.00000432
CGCTAAA207.032838E-445.00000426
TATTCGA207.032838E-445.00000414
TCCGTTA302.165214E-645.00000427
ACGTCGA207.032838E-445.00000421
CTCGATT207.032838E-445.00000414
CGGTACC207.032838E-445.00000423