##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552580_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	997990
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.735400154310163	31.0	31.0	34.0	30.0	34.0
2	31.878520826861994	33.0	31.0	34.0	30.0	34.0
3	31.9243810058217	33.0	31.0	34.0	30.0	34.0
4	35.55285523903045	37.0	35.0	37.0	33.0	37.0
5	35.37243759957514	37.0	35.0	37.0	33.0	37.0
6	35.37887153177888	37.0	35.0	37.0	33.0	37.0
7	35.75012274672091	37.0	35.0	37.0	35.0	37.0
8	35.57479533863065	37.0	35.0	37.0	35.0	37.0
9	37.325562380384575	39.0	37.0	39.0	34.0	39.0
10	36.81799416827824	39.0	37.0	39.0	32.0	39.0
11	36.698707401877776	39.0	35.0	39.0	32.0	39.0
12	36.56875920600407	39.0	35.0	39.0	32.0	39.0
13	36.34937624625497	39.0	35.0	39.0	32.0	39.0
14	37.46210883876592	39.0	36.0	41.0	32.0	41.0
15	37.628645577610996	39.0	36.0	41.0	33.0	41.0
16	37.68472930590487	40.0	36.0	41.0	33.0	41.0
17	37.662635898155294	39.0	36.0	41.0	33.0	41.0
18	37.65284121083378	39.0	36.0	41.0	33.0	41.0
19	37.63460455515586	39.0	36.0	41.0	33.0	41.0
20	37.54690628162607	39.0	36.0	41.0	33.0	41.0
21	37.4730347999479	39.0	35.0	41.0	32.0	41.0
22	37.477502780588985	39.0	35.0	41.0	32.0	41.0
23	37.390757422419064	39.0	35.0	41.0	32.0	41.0
24	37.38595677311396	39.0	35.0	41.0	32.0	41.0
25	37.22725578412609	39.0	35.0	41.0	32.0	41.0
26	37.208237557490556	39.0	35.0	41.0	32.0	41.0
27	36.96135632621569	39.0	35.0	41.0	31.0	41.0
28	36.97307788655197	39.0	35.0	41.0	31.0	41.0
29	36.989937774927604	39.0	35.0	41.0	31.0	41.0
30	36.857450475455664	39.0	35.0	41.0	31.0	41.0
31	36.82687501878776	39.0	35.0	41.0	31.0	41.0
32	36.73297628232748	39.0	35.0	41.0	31.0	41.0
33	36.634568482650124	39.0	35.0	41.0	31.0	41.0
34	36.56698664315274	39.0	35.0	40.0	31.0	41.0
35	36.45335524404052	39.0	35.0	40.0	30.0	41.0
36	36.39352799126244	38.0	35.0	40.0	30.0	41.0
37	36.347546568602894	38.0	35.0	40.0	30.0	41.0
38	36.2570877463702	38.0	35.0	40.0	30.0	41.0
39	36.23496828625537	38.0	35.0	40.0	30.0	41.0
40	36.118932053427386	38.0	35.0	40.0	30.0	41.0
41	36.029846992454836	38.0	35.0	40.0	30.0	41.0
42	35.98819627451177	38.0	35.0	40.0	30.0	41.0
43	35.934108558201984	38.0	35.0	40.0	30.0	41.0
44	35.84503251535587	38.0	35.0	40.0	30.0	41.0
45	35.79257507590256	38.0	35.0	40.0	29.0	41.0
46	35.704140322047316	38.0	34.0	40.0	29.0	41.0
47	35.59587270413531	37.0	34.0	40.0	29.0	41.0
48	35.53483501838696	37.0	34.0	40.0	29.0	41.0
49	35.47871822362949	37.0	34.0	40.0	29.0	41.0
50	35.36880028858005	37.0	34.0	40.0	29.0	41.0
51	34.66583733303941	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	45.0
10	54.0
11	72.0
12	55.0
13	50.0
14	62.0
15	70.0
16	113.0
17	220.0
18	403.0
19	662.0
20	1092.0
21	1647.0
22	2451.0
23	3481.0
24	4791.0
25	6460.0
26	8355.0
27	10152.0
28	12522.0
29	15579.0
30	19985.0
31	25651.0
32	32900.0
33	45718.0
34	75316.0
35	95052.0
36	92080.0
37	126626.0
38	187861.0
39	228362.0
40	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.68822332889107	19.406406877824427	23.754847242958345	12.150522550326155
2	30.27755789136164	24.89213318770729	26.2609845790038	18.569324341927274
3	31.985490836581533	24.608162406436936	26.126514293730395	17.279832463251136
4	28.925740738885157	26.478121023256744	25.91268449583663	18.683453742021463
5	26.00146294051043	29.992585096043044	23.332899127245767	20.673052836200764
6	25.16297758494574	36.68012705538132	22.64812272668063	15.508772632992315
7	81.58318219621438	6.515295744446337	7.485245343139711	4.416276716199561
8	83.31165642942314	5.519393981903626	6.328119520235674	4.840830068437559
9	78.38034449242978	6.873816370905519	8.325333921181576	6.4205052154831215
10	42.732893115161474	24.952855239030452	16.162687000871752	16.15156464493632
11	31.980981773364466	25.55145843144721	21.94921792803535	20.518341867152976
12	27.786049960420446	23.031793905750558	28.631850018537257	20.550306115291736
13	24.63641920259722	26.294752452429382	29.487169210112324	19.58165913486107
14	21.396005972003728	29.032355033617574	27.97553081694205	21.596108177436648
15	20.570346396256475	25.24814877904588	32.85684225292839	21.324662571769256
16	22.166554775097946	24.500345694846644	30.869146985440736	22.463952544614678
17	22.67367408491067	24.525295844647744	26.902974979709214	25.898055090732374
18	24.443531498311607	24.54934418180543	27.80629064419483	23.200833675688134
19	24.718684555957473	26.745257968516718	25.6693954849247	22.86666199060111
20	25.97170312327779	26.42912253629796	25.58512610346797	22.01404823695628
21	25.98172326376016	25.959779156103767	26.505375805368793	21.55312177476728
22	23.46536538442269	24.893836611589297	25.6347257988557	26.006072205132313
23	23.070772252226977	27.390655216986143	25.568993677291356	23.969578853495527
24	22.838204791631178	25.51618753694927	27.4513772683093	24.19423040311025
25	22.95453862263149	26.961592801531076	26.17781741300013	23.9060511628373
26	22.504534113568273	26.89125141534484	25.31748815118388	25.286726319903003
27	23.935109570236175	26.356075712181486	26.632531388090058	23.076283329492277
28	20.917945069589873	27.706490044990428	28.08254591729376	23.293018968125935
29	22.64962574775298	24.914077295363683	26.426216695558068	26.010080261325264
30	24.051643804046133	25.552159841280975	26.57130832974278	23.82488802493011
31	24.907063197026023	27.655387328530345	23.094319582360544	24.343229892083087
32	24.79473742221866	27.06299662321266	23.7834046433331	24.358861311235582
33	24.579905610276658	25.780919648493473	24.14192526979228	25.497249471437588
34	22.387498872734195	26.787943766971612	25.72981693203339	25.094740428260803
35	23.520776761290193	24.81297407789657	25.817793765468593	25.848455395344644
36	26.089740378160105	27.07792663253139	24.71207126323911	22.120261726069398
37	23.948436357077725	27.884848545576606	25.779717231635583	22.386997865710075
38	22.63599835669696	27.620116434032404	26.09404903856752	23.649836170703114
39	24.21126464193028	25.481016843856153	25.88713313760659	24.42058537660698
40	23.81687191254421	24.98692371667051	28.61521658533653	22.58098778544875
41	22.232387098067115	25.130612531187683	27.260794196334633	25.376206174410566
42	23.364963576789346	25.29744787021914	26.50898305594244	24.828605497049068
43	23.71807332738805	23.563061754125794	26.80497800579164	25.913886912694515
44	23.03399833665668	25.394442830088476	27.08504093227387	24.48651790098097
45	23.382498822633494	24.71888495876712	25.615587330534375	26.28302888806501
46	24.520987184240322	25.912884898646276	25.878315413982104	23.687812503131294
47	22.829888075030812	25.525005260573753	27.637852082686198	24.007254581709237
48	22.858946482429683	24.947644765979618	27.50097696369703	24.692431787893664
49	23.988817523221677	24.177296365695046	28.494974899548094	23.338911211535184
50	22.180282367558792	24.996543051533582	28.153488511908936	24.669686068998686
51	22.453932404132306	24.432308940971353	26.272407539153697	26.841351115742647
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1438.0
1	1717.5
2	1997.0
3	2489.5
4	2982.0
5	2266.5
6	1551.0
7	1521.5
8	1492.0
9	1524.5
10	1557.0
11	1582.0
12	1607.0
13	1773.0
14	1939.0
15	2053.5
16	2168.0
17	2084.5
18	2001.0
19	2151.5
20	2302.0
21	2269.5
22	2237.0
23	2930.0
24	3623.0
25	4326.0
26	6573.0
27	8117.0
28	9709.0
29	11301.0
30	12564.5
31	13828.0
32	14241.5
33	14655.0
34	17116.0
35	19577.0
36	23303.5
37	27030.0
38	27322.0
39	27614.0
40	32404.0
41	37194.0
42	44349.0
43	51504.0
44	59033.0
45	66562.0
46	73686.0
47	80810.0
48	86026.5
49	91243.0
50	89373.0
51	87503.0
52	81182.0
53	74861.0
54	68540.0
55	62219.0
56	58178.5
57	54138.0
58	49716.5
59	45295.0
60	43641.0
61	41987.0
62	39893.0
63	37799.0
64	34573.5
65	31348.0
66	26654.5
67	21961.0
68	20361.5
69	18762.0
70	16350.0
71	13938.0
72	12255.5
73	10573.0
74	8624.0
75	5772.0
76	4869.0
77	3596.0
78	2323.0
79	1898.5
80	1474.0
81	1096.5
82	719.0
83	430.5
84	142.0
85	77.0
86	12.0
87	11.0
88	10.0
89	15.5
90	21.0
91	11.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	997990.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.344056354153032
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.19858262491924	16.578973115694755
2	10.790114423812968	4.821898495068728
3	4.06886473826383	2.727448290275795
4	1.9994419479847265	1.7870257425051301
5	1.1904508393463151	1.3299750320601422
6	0.6927203061775411	0.9286908935338274
7	0.5011573348753264	0.7838521412926007
8	0.367185765224706	0.656353554449891
9	0.30741945907768553	0.6182097946195543
>10	3.293010920508379	18.380047639403667
>50	2.0115933383888596	33.108274434381684
>100	0.5699589850037223	16.098601288886186
>500	0.005880529210355865	0.9156201402338063
>1k	0.0036187872063728403	1.265029437594251
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	3175	0.31813946031523366	RNA PCR Primer, Index 27 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG	1641	0.1644305053156845	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC	1423	0.14258659906411889	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC	1358	0.13607350775057866	No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	1334	0.13366867403480995	RNA PCR Primer, Index 38 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1333	0.13356847262998628	No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	1206	0.12084289421737693	RNA PCR Primer, Index 38 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	1046	0.10481066944558563	RNA PCR Primer, Index 27 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0020140482369563E-4	0.0	0.0	0.14148438361105822	0.0
2	1.0020140482369563E-4	0.0	0.0	0.7974027795869698	0.0
3	1.0020140482369563E-4	0.0	0.0	1.0637381136083528	0.0
4	1.0020140482369563E-4	0.0	0.0	1.4084309462018658	0.0
5	1.0020140482369563E-4	0.0	0.0	2.464453551638794	0.0
6	1.0020140482369563E-4	0.0	0.0	3.1674666078818423	0.0
7	1.0020140482369563E-4	0.0	0.0	3.835008366817303	0.0
8	1.0020140482369563E-4	0.0	0.0	4.915279712221565	0.0
9	1.0020140482369563E-4	0.0	0.0	5.378110001102216	0.0
10	1.0020140482369563E-4	0.0	0.0	6.478321426066393	0.0
11	1.0020140482369563E-4	0.0	0.0	7.5928616519203596	0.0
12	1.0020140482369563E-4	0.0	0.0	8.609805709476047	0.0
13	1.0020140482369563E-4	0.0	0.0	8.961512640407218	0.0
14	1.0020140482369563E-4	0.0	0.0	9.0954819186565	0.0
15	1.0020140482369563E-4	0.0	0.0	9.35740839086564	0.0
16	1.0020140482369563E-4	0.0	0.0	9.907914908967024	0.0
17	1.0020140482369563E-4	0.0	0.0	10.5856772111945	0.0
18	1.0020140482369563E-4	0.0	0.0	11.420354913375885	0.0
19	1.0020140482369563E-4	0.0	0.0	11.851220954117776	0.0
20	1.0020140482369563E-4	0.0	0.0	12.224871992705337	0.0
21	1.0020140482369563E-4	0.0	0.0	12.685197246465396	0.0
22	1.0020140482369563E-4	0.0	0.0	13.16576318399984	0.0
23	1.0020140482369563E-4	0.0	0.0	13.635507369813325	0.0
24	2.0040280964739126E-4	0.0	0.0	14.018176534835018	0.0
25	2.0040280964739126E-4	0.0	0.0	14.349141774967684	0.0
26	2.0040280964739126E-4	0.0	0.0	14.665076804376797	0.0
27	2.0040280964739126E-4	0.0	0.0	14.994338620627461	0.0
28	2.0040280964739126E-4	0.0	0.0	15.314582310443992	0.0
29	2.0040280964739126E-4	0.0	0.0	15.6444453351236	0.0
30	2.0040280964739126E-4	0.0	0.0	16.034028397078128	0.0
31	3.006042144710869E-4	0.0	0.0	16.38523432098518	0.0
32	5.010070241184782E-4	0.0	0.0	16.72171063838315	0.0
33	5.010070241184782E-4	0.0	0.0	17.063698033046425	0.0
34	5.010070241184782E-4	0.0	0.0	17.395064078798384	0.0
35	5.010070241184782E-4	0.0	0.0	17.79566929528352	0.0
36	5.010070241184782E-4	0.0	0.0	18.1313440014429	0.0
37	6.012084289421738E-4	0.0	0.0	18.4578001783585	0.0
38	7.014098337658694E-4	0.0	0.0	18.784156153869276	0.0
39	7.014098337658694E-4	0.0	0.0	19.137666710087274	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTA	20	7.032838E-4	45.000004	17
CGTATGG	140	0.0	45.000004	2
GTCGACA	20	7.032838E-4	45.000004	18
CACGGTA	20	7.032838E-4	45.000004	39
CTCCGAT	20	7.032838E-4	45.000004	23
TTTACGT	30	2.165214E-6	45.000004	17
ACGTTAG	35	1.2117198E-7	45.000004	1
ATAATCG	20	7.032838E-4	45.000004	40
GACGTAC	20	7.032838E-4	45.000004	27
ATTACCG	20	7.032838E-4	45.000004	20
CGCGATT	20	7.032838E-4	45.000004	13
CGTTACG	20	7.032838E-4	45.000004	45
ACGATTG	20	7.032838E-4	45.000004	34
CGTAGTA	20	7.032838E-4	45.000004	32
CGCTAAA	20	7.032838E-4	45.000004	26
TATTCGA	20	7.032838E-4	45.000004	14
TCCGTTA	30	2.165214E-6	45.000004	27
ACGTCGA	20	7.032838E-4	45.000004	21
CTCGATT	20	7.032838E-4	45.000004	14
CGGTACC	20	7.032838E-4	45.000004	23
>>END_MODULE