FastQCFastQC Report
Sat 18 Jun 2016
SRR3552579_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552579_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928768
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC40580.4369228914002205No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC29480.31740972987872107No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT23980.2581914966923925No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC16140.17377859702315324No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14060.15138333792723266No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13380.14406181091510475No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC10920.11757511025358323No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC9900.1065928197353914No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA207.0325873E-445.038
ACACGAA253.8902257E-545.024
ACGCCAA253.8902257E-545.044
CGTATAG253.8902257E-545.02
CGAGTAT207.0325873E-445.024
ACCGCGT207.0325873E-445.023
CGCATCG207.0325873E-445.021
GCGCATC253.8902257E-545.09
TAGTTCG207.0325873E-445.01
ATATCGT207.0325873E-445.017
CACTCGA207.0325873E-445.016
TCTACCG253.8902257E-545.022
TCGCTAA207.0325873E-445.021
ACTCGCA207.0325873E-445.027
ACTCGAT207.0325873E-445.017
CATCGTT207.0325873E-445.036
TTACGAG502.1827873E-1145.01
ACGTATA253.8902257E-545.020
CAACTCG207.0325873E-445.036
ACTCACG253.8902257E-545.013