##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552579_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	928768
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.660888402701215	31.0	31.0	34.0	30.0	34.0
2	31.805884785005514	33.0	31.0	34.0	30.0	34.0
3	31.861128936397463	33.0	31.0	34.0	30.0	34.0
4	35.493576436742	37.0	35.0	37.0	33.0	37.0
5	35.31556104430816	37.0	35.0	37.0	33.0	37.0
6	35.322810432745314	37.0	35.0	37.0	32.0	37.0
7	35.75185191565601	37.0	35.0	37.0	35.0	37.0
8	35.5864952280871	37.0	35.0	37.0	35.0	37.0
9	37.302692383889195	39.0	37.0	39.0	34.0	39.0
10	36.79186729086273	39.0	37.0	39.0	32.0	39.0
11	36.708757192323596	39.0	35.0	39.0	32.0	39.0
12	36.535394199627895	39.0	35.0	39.0	32.0	39.0
13	36.30885538692117	39.0	35.0	39.0	32.0	39.0
14	37.374725442737045	39.0	36.0	41.0	32.0	41.0
15	37.52827293791345	39.0	36.0	41.0	32.0	41.0
16	37.602752248139474	39.0	36.0	41.0	33.0	41.0
17	37.580231015711135	39.0	36.0	41.0	33.0	41.0
18	37.591283291414	39.0	36.0	41.0	33.0	41.0
19	37.55620564015987	39.0	36.0	41.0	33.0	41.0
20	37.47769733668688	39.0	35.0	41.0	32.0	41.0
21	37.36603435949559	39.0	35.0	41.0	32.0	41.0
22	37.39812095162624	39.0	35.0	41.0	32.0	41.0
23	37.32062689498346	39.0	35.0	41.0	32.0	41.0
24	37.29548929334344	39.0	35.0	41.0	32.0	41.0
25	37.14349654596196	39.0	35.0	41.0	32.0	41.0
26	37.148454727122385	39.0	35.0	41.0	32.0	41.0
27	36.94577870899945	39.0	35.0	41.0	31.0	41.0
28	36.990716734426684	39.0	35.0	41.0	31.0	41.0
29	37.00231920135061	39.0	35.0	41.0	31.0	41.0
30	36.85692013506064	39.0	35.0	40.0	31.0	41.0
31	36.86105679782249	39.0	35.0	40.0	31.0	41.0
32	36.758857970989524	39.0	35.0	40.0	31.0	41.0
33	36.66249698525358	39.0	35.0	40.0	31.0	41.0
34	36.601736924614116	39.0	35.0	40.0	31.0	41.0
35	36.49901267054851	39.0	35.0	40.0	30.0	41.0
36	36.4288961290656	38.0	35.0	40.0	30.0	41.0
37	36.42558313809261	38.0	35.0	40.0	30.0	41.0
38	36.30450553851985	38.0	35.0	40.0	30.0	41.0
39	36.30120869797409	38.0	35.0	40.0	30.0	41.0
40	36.18784454244763	38.0	35.0	40.0	30.0	41.0
41	36.10845873242833	38.0	35.0	40.0	30.0	41.0
42	36.094441238285555	38.0	35.0	40.0	30.0	41.0
43	36.04145168653528	38.0	35.0	40.0	30.0	41.0
44	35.94946208310364	38.0	35.0	40.0	30.0	41.0
45	35.89123763953969	38.0	35.0	40.0	30.0	41.0
46	35.77536801440188	38.0	35.0	40.0	30.0	41.0
47	35.72311276874311	37.0	35.0	40.0	30.0	41.0
48	35.69131042413175	37.0	35.0	40.0	30.0	41.0
49	35.63464611183848	37.0	34.0	40.0	29.0	41.0
50	35.56065562121003	37.0	34.0	40.0	29.0	41.0
51	34.875117359771224	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	25.0
10	46.0
11	41.0
12	41.0
13	35.0
14	37.0
15	60.0
16	106.0
17	191.0
18	348.0
19	529.0
20	914.0
21	1408.0
22	2184.0
23	3026.0
24	4193.0
25	5737.0
26	7675.0
27	9274.0
28	11755.0
29	14466.0
30	18328.0
31	23958.0
32	31248.0
33	42795.0
34	71369.0
35	91338.0
36	84584.0
37	117242.0
38	175353.0
39	210333.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.97822491730982	19.56484288864388	24.24114525909592	13.215786934950385
2	30.245120417585447	24.81545445148842	25.189498346196252	19.74992678472988
3	32.50036607635061	24.730718543274534	25.540285625689087	17.22862975468578
4	28.468250413450935	26.201268777563396	25.06837014195149	20.26211066703418
5	24.006533386163177	30.532275013781696	24.73760939222712	20.723582207828002
6	24.67171564911797	34.152339443219404	24.822345300441015	16.353599607221607
7	81.54921358186328	5.866911866041896	7.808731566979052	4.7751429851157665
8	81.75626205898567	4.79430815876516	7.443839581036384	6.005590201212789
9	76.16412279492833	6.9038769638919515	10.380525599503859	6.5514746416758545
10	41.09594645810364	24.869612217475193	17.536241558710035	16.498199765711135
11	31.696720817254686	25.39977690876516	22.55299493522602	20.350507338754134
12	28.13619762954796	21.881244831863285	28.917232290518193	21.06532524807056
13	23.2920384853914	26.396581277563396	29.986390573318634	20.32498966372657
14	19.97064929024256	28.81731498070562	28.817099641675853	22.394936087375964
15	19.076992316703418	25.111007269845647	34.634160522326354	21.177839891124588
16	20.756421409867695	24.676883785832416	31.998087789415656	22.568607014884236
17	20.09683796168688	24.908050234288865	28.209628238699008	26.785483565325247
18	22.837996141124588	24.275599503858874	28.922400427232635	23.964003927783903
19	23.245632924476293	26.46861218302095	26.164445803472987	24.12130908902977
20	25.937693805126795	25.395900806229328	26.10167447629548	22.564730912348402
21	23.81972677783903	27.291637954796034	26.361804024255786	22.526831243109154
22	22.603599607221607	25.572048132579933	24.773032662624036	27.05131959757442
23	21.356032938257993	28.086023635611905	25.054480774531424	25.503462651598678
24	22.124793274531424	25.915621554575523	27.727376481532524	24.23220868936053
25	21.55252980292172	26.66037158902977	27.014065945424477	24.773032662624036
26	20.0891934261301	26.443848194597575	27.712841097023155	25.754117282249172
27	21.556298235942666	26.056130271499452	27.64134853914002	24.746222953417863
28	19.828202522050717	26.040195183296582	30.280759027012127	23.850843267640574
29	20.75965149531422	25.346157490352812	27.53217165104741	26.362019363285555
30	22.04253376515987	25.980438602535834	26.91091855016538	25.066109082138922
31	22.465890297684673	26.130745245314223	25.687146843991183	25.716217613009924
32	22.847686397464166	25.788679196527013	26.273192013506062	25.090442392502755
33	21.279264574145536	25.79653907111356	27.009005478224918	25.915190876515986
34	19.65786934950386	25.20877118936053	28.391912727398015	26.741446733737597
35	19.626322181642777	24.146934433572216	28.660440497519296	27.56630288726571
36	22.222880202590957	25.870400358324147	28.06481274117971	23.841906697905184
37	20.23034816014333	25.600580554024255	30.29077229189636	23.878298993936053
38	20.25220507166483	27.579653907111357	28.303516055678056	23.864624965545755
39	22.230955416207276	25.112299304024255	28.51616334757442	24.140581932194046
40	23.093280560915105	23.679433399944873	27.75397085170893	25.47331518743109
41	19.30439033213892	23.634427542723262	27.25266159040794	29.808520534729876
42	20.84503342061742	23.389371726846747	28.44240972987872	27.32318512265711
43	21.15662899669239	22.989271809536934	30.022890538864388	25.831208654906284
44	21.117867971334068	24.81534678197354	28.484077832138922	25.582707414553475
45	21.95715183985667	23.91329158627343	27.017296030871	27.112260542998897
46	22.320644122105843	24.911387989250276	27.181922719129	25.586045169514883
47	20.005211204520396	24.091699972436604	29.94666052232635	25.95642830071665
48	21.26903597023153	23.258768605292172	28.56676801957001	26.905427404906284
49	21.312319115214994	22.5606394707828	31.028954485942666	25.098086928059537
50	20.91577229189636	22.85845334895259	29.99597316014333	26.229801199007717
51	20.60525341097023	23.052689153803748	26.865697353914005	29.47636008131202
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1754.0
1	1878.0
2	2002.0
3	2540.5
4	3079.0
5	2301.5
6	1524.0
7	1533.0
8	1542.0
9	1582.5
10	1623.0
11	1593.0
12	1563.0
13	1746.5
14	1930.0
15	1763.0
16	1596.0
17	1942.5
18	2289.0
19	2360.5
20	2432.0
21	2409.0
22	2386.0
23	2479.0
24	2572.0
25	3505.0
26	5290.0
27	6142.0
28	8085.0
29	10028.0
30	10919.5
31	11811.0
32	11954.5
33	12098.0
34	14653.0
35	17208.0
36	19312.0
37	21416.0
38	23834.5
39	26253.0
40	31947.5
41	37642.0
42	45707.0
43	53772.0
44	65496.0
45	77220.0
46	87059.5
47	96899.0
48	96853.0
49	96807.0
50	86123.5
51	75440.0
52	67986.5
53	60533.0
54	57305.5
55	54078.0
56	49721.0
57	45364.0
58	42459.5
59	39555.0
60	37443.0
61	35331.0
62	33325.0
63	31319.0
64	28393.0
65	25467.0
66	22124.0
67	18781.0
68	16495.5
69	14210.0
70	11859.5
71	9509.0
72	8721.0
73	7933.0
74	6712.0
75	4719.5
76	3948.0
77	3045.5
78	2143.0
79	1538.5
80	934.0
81	653.5
82	373.0
83	231.0
84	89.0
85	132.0
86	175.0
87	113.0
88	51.0
89	29.0
90	7.0
91	5.0
92	3.0
93	2.5
94	2.0
95	3.5
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	928768.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.297737997406436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.81399241047303	17.692153101348143
2	11.256688241842841	5.470241232375661
3	4.617400929512725	3.3657719404284343
4	2.3650257503054433	2.2985910415216493
5	1.3330358638467152	1.6194878080446924
6	0.8894584306380581	1.2967096744336704
7	0.6419262077075755	1.091814836598316
8	0.4780076701843223	0.9291604104711464
9	0.3252603387700774	0.7112781443144698
>10	3.1134914056483862	17.470457893851936
>50	1.6013852898599104	28.594645478444892
>100	0.5562847895547085	17.055278113411898
>500	0.004914966012129955	0.7917715491235485
>1k	0.003127705644082699	1.6126387756315905
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	4058	0.4369228914002205	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	2948	0.31740972987872107	No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	2398	0.2581914966923925	No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1614	0.17377859702315324	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1406	0.15138333792723266	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1338	0.14406181091510475	No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1092	0.11757511025358323	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	990	0.1065928197353914	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2721885336273429	0.0
2	1.0766951488423373E-4	0.0	0.0	1.3810768674200662	0.0
3	1.0766951488423373E-4	0.0	0.0	1.7870986080485116	0.0
4	1.0766951488423373E-4	0.0	0.0	2.2931453280044103	0.0
5	1.0766951488423373E-4	0.0	0.0	3.7691867075523704	0.0
6	1.0766951488423373E-4	0.0	0.0	4.828547064498347	0.0
7	2.1533902976846747E-4	0.0	0.0	5.827935501653804	0.0
8	2.1533902976846747E-4	0.0	0.0	7.460420686328556	0.0
9	2.1533902976846747E-4	0.0	0.0	8.12377256753032	0.0
10	2.1533902976846747E-4	0.0	0.0	9.476424683020948	0.0
11	2.1533902976846747E-4	0.0	0.0	11.034617902425579	0.0
12	2.1533902976846747E-4	0.0	0.0	12.29198249724366	0.0
13	2.1533902976846747E-4	0.0	0.0	12.75862217475193	0.0
14	2.1533902976846747E-4	0.0	0.0	12.922495176405732	0.0
15	2.1533902976846747E-4	0.0	0.0	13.239366558710033	0.0
16	2.1533902976846747E-4	0.0	0.0	13.942556160418963	0.0
17	2.1533902976846747E-4	0.0	0.0	14.840842923098126	1.0766951488423373E-4
18	2.1533902976846747E-4	0.0	0.0	15.916353707276736	1.0766951488423373E-4
19	3.230085446527012E-4	0.0	0.0	16.437366489801544	1.0766951488423373E-4
20	3.230085446527012E-4	0.0	0.0	16.922309984840133	1.0766951488423373E-4
21	3.230085446527012E-4	0.0	0.0	17.546254823594268	1.0766951488423373E-4
22	3.230085446527012E-4	0.0	0.0	18.124332449007717	1.0766951488423373E-4
23	3.230085446527012E-4	0.0	0.0	18.732557538588754	1.0766951488423373E-4
24	3.230085446527012E-4	0.0	0.0	19.235697181642777	1.0766951488423373E-4
25	3.230085446527012E-4	0.0	0.0	19.671866386438808	1.0766951488423373E-4
26	3.230085446527012E-4	0.0	0.0	20.069059226846747	1.0766951488423373E-4
27	4.3067805953693494E-4	0.0	0.0	20.49715321802646	1.0766951488423373E-4
28	4.3067805953693494E-4	0.0	0.0	20.904897670893053	1.0766951488423373E-4
29	4.3067805953693494E-4	0.0	0.0	21.318994625137815	1.0766951488423373E-4
30	4.3067805953693494E-4	0.0	0.0	21.79747794928335	1.0766951488423373E-4
31	4.3067805953693494E-4	0.0	0.0	22.24732118246968	1.0766951488423373E-4
32	5.383475744211687E-4	0.0	0.0	22.684136404355016	1.0766951488423373E-4
33	7.536866041896362E-4	0.0	0.0	23.123535694597575	1.0766951488423373E-4
34	7.536866041896362E-4	0.0	0.0	23.54861493936053	1.0766951488423373E-4
35	7.536866041896362E-4	0.0	0.0	23.99673546030871	1.0766951488423373E-4
36	7.536866041896362E-4	0.0	0.0	24.416538898842337	1.0766951488423373E-4
37	7.536866041896362E-4	0.0	0.0	24.831066531146636	1.0766951488423373E-4
38	7.536866041896362E-4	0.0	0.0	25.22965907524807	1.0766951488423373E-4
39	7.536866041896362E-4	0.0	0.0	25.61543894707828	1.0766951488423373E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	20	7.0325873E-4	45.0	38
ACACGAA	25	3.8902257E-5	45.0	24
ACGCCAA	25	3.8902257E-5	45.0	44
CGTATAG	25	3.8902257E-5	45.0	2
CGAGTAT	20	7.0325873E-4	45.0	24
ACCGCGT	20	7.0325873E-4	45.0	23
CGCATCG	20	7.0325873E-4	45.0	21
GCGCATC	25	3.8902257E-5	45.0	9
TAGTTCG	20	7.0325873E-4	45.0	1
ATATCGT	20	7.0325873E-4	45.0	17
CACTCGA	20	7.0325873E-4	45.0	16
TCTACCG	25	3.8902257E-5	45.0	22
TCGCTAA	20	7.0325873E-4	45.0	21
ACTCGCA	20	7.0325873E-4	45.0	27
ACTCGAT	20	7.0325873E-4	45.0	17
CATCGTT	20	7.0325873E-4	45.0	36
TTACGAG	50	2.1827873E-11	45.0	1
ACGTATA	25	3.8902257E-5	45.0	20
CAACTCG	20	7.0325873E-4	45.0	36
ACTCACG	25	3.8902257E-5	45.0	13
>>END_MODULE