FastQCFastQC Report
Sat 18 Jun 2016
SRR3552574_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552574_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379294
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24720.17922212378216681No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC24620.17849711519081501No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGC22680.16443194851858994No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCG22670.16435944765945476No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC21070.15275931019782585TruSeq Adapter, Index 20 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC20990.15217930332474439No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15920.11542136774320776No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC14710.10664876378785088TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATA207.0337777E-445.015
CGGTCTA406.8175723E-945.031
CGTTACA253.891214E-544.99999639
GGACGTA556.184564E-1140.9090928
ATAACGG556.184564E-1140.9090922
TAGGACG501.0822987E-940.4999961
TAAGACG501.0822987E-940.4999961
ACGTAAC356.249198E-638.5714316
CGTAAGG2200.037.8409082
TACGGGA3400.037.720594
ACGCATT850.037.05882317
TATGCGG1700.037.0588232
GCGTAAG1700.037.0588231
CGAGGGA7550.036.9536444
CGTTGAT2700.036.66666425
AAGGGTA4000.036.5624965
ATTCGGT250.00210725535.99999613
CGTACTC250.00210725535.99999645
GTATACG250.00210725535.9999961
GACGATA250.00210725535.99999637