##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552574_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1379294
Sequences flagged as poor quality	0
Sequence length	51
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.782769300816216	31.0	31.0	34.0	30.0	34.0
2	31.930483276226823	33.0	31.0	34.0	30.0	34.0
3	31.978562220962317	33.0	31.0	34.0	30.0	34.0
4	35.611326519219254	37.0	35.0	37.0	33.0	37.0
5	35.41600340464034	37.0	35.0	37.0	33.0	37.0
6	35.419313793868454	37.0	35.0	37.0	33.0	37.0
7	35.74839519348304	37.0	35.0	37.0	35.0	37.0
8	35.55079410191011	37.0	35.0	37.0	35.0	37.0
9	37.29973232682807	39.0	37.0	39.0	34.0	39.0
10	36.84932291447654	39.0	37.0	39.0	32.0	39.0
11	36.749876386035176	39.0	37.0	39.0	32.0	39.0
12	36.62940968350475	39.0	35.0	39.0	32.0	39.0
13	36.48092937401308	39.0	35.0	39.0	32.0	39.0
14	37.628908702568125	40.0	36.0	41.0	33.0	41.0
15	37.76226243280983	40.0	36.0	41.0	33.0	41.0
16	37.800442110239004	40.0	36.0	41.0	33.0	41.0
17	37.7350187849726	40.0	36.0	41.0	33.0	41.0
18	37.74305115515619	40.0	36.0	41.0	33.0	41.0
19	37.72923611644798	40.0	36.0	41.0	33.0	41.0
20	37.651154141176576	40.0	36.0	41.0	33.0	41.0
21	37.56943189776799	39.0	36.0	41.0	32.0	41.0
22	37.589154306478534	39.0	36.0	41.0	33.0	41.0
23	37.46790314465226	39.0	35.0	41.0	32.0	41.0
24	37.48416146231333	39.0	35.0	41.0	32.0	41.0
25	37.33550352571678	39.0	35.0	41.0	32.0	41.0
26	37.30485016247442	39.0	35.0	41.0	32.0	41.0
27	37.07104794191811	39.0	35.0	41.0	31.0	41.0
28	37.06920932013044	39.0	35.0	41.0	31.0	41.0
29	37.03852623153584	39.0	35.0	41.0	31.0	41.0
30	36.91671826311142	39.0	35.0	41.0	31.0	41.0
31	36.93066742840902	39.0	35.0	41.0	31.0	41.0
32	36.80226768187203	39.0	35.0	41.0	31.0	41.0
33	36.671279654663905	39.0	35.0	41.0	31.0	41.0
34	36.602010883828974	39.0	35.0	41.0	30.0	41.0
35	36.454098981072924	39.0	35.0	41.0	30.0	41.0
36	36.391240736202725	39.0	35.0	40.0	30.0	41.0
37	36.32907777457163	38.0	35.0	40.0	30.0	41.0
38	36.246122291549156	38.0	35.0	40.0	30.0	41.0
39	36.20286682897192	38.0	35.0	40.0	30.0	41.0
40	36.12423964723982	38.0	35.0	40.0	30.0	41.0
41	36.056768172702846	38.0	35.0	40.0	30.0	41.0
42	36.01668462271278	38.0	35.0	40.0	30.0	41.0
43	35.93629929514665	38.0	35.0	40.0	30.0	41.0
44	35.847065962731655	38.0	35.0	40.0	29.0	41.0
45	35.771217013921614	38.0	35.0	40.0	29.0	41.0
46	35.65175372328162	38.0	34.0	40.0	29.0	41.0
47	35.55745693086463	37.0	34.0	40.0	29.0	41.0
48	35.49858623324686	37.0	34.0	40.0	29.0	41.0
49	35.41839448297463	37.0	34.0	40.0	29.0	41.0
50	35.343799074019024	37.0	34.0	40.0	28.0	41.0
51	34.65477048403024	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	20.0
9	55.0
10	95.0
11	101.0
12	74.0
13	77.0
14	83.0
15	124.0
16	186.0
17	296.0
18	485.0
19	857.0
20	1483.0
21	2392.0
22	3582.0
23	5261.0
24	7036.0
25	9007.0
26	11755.0
27	14748.0
28	17605.0
29	21838.0
30	27090.0
31	34633.0
32	44428.0
33	60725.0
34	98841.0
35	121008.0
36	126922.0
37	178873.0
38	260639.0
39	328791.0
40	181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.621813768493155	19.102526364937425	22.792385089763314	13.483274776806105
2	29.89681677727881	25.54973776439251	25.208258717865807	19.345186740462875
3	32.5375155695595	25.05114935611987	25.456284156967264	16.955050917353372
4	28.733612993313972	26.324554445970183	25.803273268788235	19.13855929192761
5	25.671539207739613	30.2537385068013	23.733373740478825	20.34134854498026
6	25.254369264275784	36.177131199004705	22.65042840757663	15.91807112914288
7	82.28630009265609	6.124002569430448	7.429888044173323	4.159809293740131
8	83.85260865341255	5.835231647495022	5.973780789302354	4.338378909790081
9	78.94930304924114	6.40378338483311	7.988217160373351	6.658696405552406
10	43.279242859027875	25.4932595951262	15.199007608240159	16.028489937605762
11	32.57760854466125	26.220878217406877	20.36491132419919	20.83660191373268
12	27.732158626079716	23.087753589880037	27.390607078693886	21.78948070534636
13	25.525014971427414	24.301925477816912	28.639289375579104	21.533770175176574
14	22.031271070562187	26.68423120813982	27.871215273900994	23.413282447397002
15	21.89989951380924	24.9201403036626	29.95604997919225	23.223910203335908
16	24.0947905232677	24.012284545571866	28.64697446664743	23.245950464513005
17	23.46555556683347	24.34259845979175	27.490005756568216	24.70184021680657
18	25.227616447254903	23.654420304880613	26.86860089292058	24.24936235494391
19	25.454109131193203	25.691621945720055	24.630136867121877	24.224132055964866
20	27.27525821180981	24.848581955696176	25.24479915086994	22.631360681624077
21	25.94000988911719	27.090308520155965	24.791233776120247	22.178447814606603
22	24.831979258954217	23.27777834167335	25.868886546305575	26.021355853066858
23	24.114873261248146	26.715551579286213	25.27205947390477	23.897515685560876
24	23.566331761031368	25.010186370708492	27.22305759323248	24.20042427502766
25	24.247984838620336	26.68684123906868	25.01156388703206	24.05361003527892
26	23.293946033260493	25.985757931231486	25.52958252555293	25.190713509955092
27	24.90071007341437	24.77724111030716	25.74752010811328	24.574528708165193
28	22.458373631727536	26.230665833390127	27.171219478950825	24.139741055931513
29	24.471722489911503	25.67915179794881	25.37595320504548	24.47317250709421
30	24.13901604734016	26.030128457022215	26.035348518879946	23.795506976757675
31	25.709022151912503	25.792615642495363	23.511521111525173	24.986841094066964
32	25.646816414774516	26.477096253590602	23.793549453561024	24.082537878073857
33	23.843792548941707	26.466511128156867	23.076660958432356	26.613035364469066
34	23.349409190498907	26.49949901906338	25.449686578785958	24.701405211651757
35	24.336798391060935	24.947690630133966	25.631591234356126	25.083919744448973
36	24.5028253584805	27.46310793782906	23.586414499011813	24.447652204678626
37	23.39943478330218	27.610502184450887	24.893532488359988	24.096530543886946
38	23.068540862209215	27.990986693192315	23.828712370241586	25.11176007435688
39	23.449315374387186	26.276196372927018	25.034474158518776	25.24001409416702
40	24.534435733063436	25.060356965230042	26.497976501021537	23.90723080068499
41	21.532610161430412	25.10102994720487	26.006348175225874	27.36001171613884
42	22.662536051052204	25.036504182574564	25.49347709770361	26.807482668669625
43	23.349264188780637	24.014024566191107	25.876281633937364	26.760429611090892
44	23.196142374287135	24.732073075065937	26.35790484117237	25.713879709474558
45	24.34259845979175	23.96798652064027	25.38922086226722	26.300194157300766
46	24.05854009370011	25.548505249787212	25.14431296010858	25.2486416964041
47	22.79572012928353	25.484341989452574	27.6529876879041	24.06695019335979
48	22.83806063101848	25.39306340780138	26.356164820553126	25.412711140627014
49	22.859955890477302	25.836768665708686	26.899631260630443	24.40364418318357
50	22.52471191783623	24.7383806498107	27.22711764134405	25.509789791009023
51	22.072234055973563	24.641592002865234	26.237335912430567	27.048838028730643
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1082.0
1	1556.0
2	2030.0
3	3511.0
4	4992.0
5	3643.0
6	2294.0
7	2212.0
8	2130.0
9	2143.5
10	2157.0
11	2122.5
12	2088.0
13	2203.5
14	2319.0
15	2355.5
16	2392.0
17	2482.0
18	2572.0
19	2726.5
20	2881.0
21	3005.5
22	3130.0
23	3516.0
24	3902.0
25	5345.0
26	8021.0
27	9254.0
28	11469.5
29	13685.0
30	16910.5
31	20136.0
32	21569.0
33	23002.0
34	27055.0
35	31108.0
36	33481.5
37	35855.0
38	36812.0
39	37769.0
40	43033.5
41	48298.0
42	53949.5
43	59601.0
44	66880.5
45	74160.0
46	87178.5
47	100197.0
48	105606.0
49	111015.0
50	111721.0
51	112427.0
52	105539.0
53	98651.0
54	93432.0
55	88213.0
56	82817.5
57	77422.0
58	76629.0
59	75836.0
60	73522.0
61	71208.0
62	66565.0
63	61922.0
64	56610.5
65	51299.0
66	45579.0
67	39859.0
68	33929.5
69	28000.0
70	25639.5
71	23279.0
72	20260.5
73	17242.0
74	15086.0
75	9967.5
76	7005.0
77	5663.5
78	4322.0
79	3487.0
80	2652.0
81	2023.5
82	1395.0
83	968.5
84	542.0
85	375.5
86	209.0
87	119.0
88	29.0
89	16.0
90	3.0
91	4.5
92	6.0
93	5.0
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1379294.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.10671113842274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.00194112916316	18.32156840760147
2	8.854883121629262	4.269242191552233
3	3.0710609417819312	2.2209953703608853
4	1.4649529083819077	1.4126078637501969
5	0.9363464879208236	1.1286117154891966
6	0.615918575887477	0.8908662716224869
7	0.4584939530610291	0.773694689960905
8	0.3874854430156004	0.7472799716096675
9	0.33319960577459207	0.7229111983059983
>10	6.2345832952127775	40.68569918717006
>50	1.564182948435101	23.729372273122117
>100	0.06907465535410284	2.8511530319195444
>500	0.004241426205836496	0.6991492603321848
>1k	0.0036355081764312823	1.5468485672031418
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2472	0.17922212378216681	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC	2462	0.17849711519081501	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGC	2268	0.16443194851858994	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCG	2267	0.16435944765945476	No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	2107	0.15275931019782585	TruSeq Adapter, Index 20 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2099	0.15217930332474439	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1592	0.11542136774320776	No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	1471	0.10664876378785088	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1750257740554225E-4	0.0	0.0	0.10295121997195666	0.0
2	2.1750257740554225E-4	0.0	0.0	0.4793756806018151	0.0
3	2.90003436540723E-4	0.0	0.0	0.630539971898667	0.0
4	2.90003436540723E-4	0.0	0.0	0.8336873791954434	0.0
5	2.90003436540723E-4	0.0	0.0	1.5417307695096187	0.0
6	2.90003436540723E-4	0.0	0.0	2.070479535182492	0.0
7	2.90003436540723E-4	0.0	0.0	2.5576128077117715	0.0
8	2.90003436540723E-4	0.0	0.0	3.3277894343047967	0.0
9	2.90003436540723E-4	0.0	0.0	3.6851461689821026	0.0
10	2.90003436540723E-4	0.0	0.0	4.59263942277716	0.0
11	2.90003436540723E-4	0.0	0.0	5.472799852678254	0.0
12	2.90003436540723E-4	0.0	0.0	6.371955507672766	0.0
13	2.90003436540723E-4	0.0	0.0	6.677836632364094	0.0
14	2.90003436540723E-4	0.0	0.0	6.814718254411315	0.0
15	2.90003436540723E-4	0.0	0.0	7.05563860931752	0.0
16	2.90003436540723E-4	0.0	0.0	7.478101115498219	7.250085913518075E-5
17	2.90003436540723E-4	0.0	0.0	7.981402079614644	7.250085913518075E-5
18	2.90003436540723E-4	0.0	0.0	8.538861185505048	7.250085913518075E-5
19	3.625042956759038E-4	0.0	0.0	8.890707854888081	7.250085913518075E-5
20	3.625042956759038E-4	0.0	0.0	9.199634015663086	7.250085913518075E-5
21	3.625042956759038E-4	0.0	0.0	9.582946057910787	7.250085913518075E-5
22	3.625042956759038E-4	0.0	0.0	9.994388433502937	7.250085913518075E-5
23	3.625042956759038E-4	0.0	0.0	10.39089563211324	7.250085913518075E-5
24	3.625042956759038E-4	0.0	0.0	10.729039639119723	7.250085913518075E-5
25	3.625042956759038E-4	0.0	0.0	11.011647988028658	7.250085913518075E-5
26	3.625042956759038E-4	0.0	0.0	11.274898607548499	7.250085913518075E-5
27	3.625042956759038E-4	0.0	0.0	11.545906818995805	7.250085913518075E-5
28	4.350051548110845E-4	0.0	0.0	11.823657610342682	7.250085913518075E-5
29	4.350051548110845E-4	0.0	0.0	12.112936038292053	7.250085913518075E-5
30	4.350051548110845E-4	0.0	0.0	12.451515050453349	7.250085913518075E-5
31	5.075060139462653E-4	0.0	0.0	12.739633464656556	7.250085913518075E-5
32	5.075060139462653E-4	0.0	0.0	13.063567303272544	7.250085913518075E-5
33	5.075060139462653E-4	0.0	0.0	13.36872341937252	7.250085913518075E-5
34	5.075060139462653E-4	0.0	0.0	13.664454423784921	7.250085913518075E-5
35	5.075060139462653E-4	0.0	0.0	13.989403274428803	7.250085913518075E-5
36	5.075060139462653E-4	0.0	0.0	14.277739191209417	7.250085913518075E-5
37	5.075060139462653E-4	0.0	0.0	14.57238268273479	7.250085913518075E-5
38	5.075060139462653E-4	0.0	0.0	14.868403690583733	7.250085913518075E-5
39	5.075060139462653E-4	0.0	0.0	15.16616471905192	7.250085913518075E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAATA	20	7.0337777E-4	45.0	15
CGGTCTA	40	6.8175723E-9	45.0	31
CGTTACA	25	3.891214E-5	44.999996	39
GGACGTA	55	6.184564E-11	40.909092	8
ATAACGG	55	6.184564E-11	40.909092	2
TAGGACG	50	1.0822987E-9	40.499996	1
TAAGACG	50	1.0822987E-9	40.499996	1
ACGTAAC	35	6.249198E-6	38.57143	16
CGTAAGG	220	0.0	37.840908	2
TACGGGA	340	0.0	37.72059	4
ACGCATT	85	0.0	37.058823	17
TATGCGG	170	0.0	37.058823	2
GCGTAAG	170	0.0	37.058823	1
CGAGGGA	755	0.0	36.953644	4
CGTTGAT	270	0.0	36.666664	25
AAGGGTA	400	0.0	36.562496	5
ATTCGGT	25	0.002107255	35.999996	13
CGTACTC	25	0.002107255	35.999996	45
GTATACG	25	0.002107255	35.999996	1
GACGATA	25	0.002107255	35.999996	37
>>END_MODULE