##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552573_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	619539
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86327091595525	31.0	31.0	34.0	30.0	34.0
2	31.999664266494925	33.0	31.0	34.0	30.0	34.0
3	32.035038956385314	33.0	31.0	34.0	30.0	34.0
4	35.66768355180223	37.0	35.0	37.0	33.0	37.0
5	35.49406736299087	37.0	35.0	37.0	33.0	37.0
6	35.48965440432321	37.0	35.0	37.0	33.0	37.0
7	35.81768702212452	37.0	35.0	37.0	35.0	37.0
8	35.65255940304	37.0	35.0	37.0	35.0	37.0
9	37.382515063619884	39.0	37.0	39.0	35.0	39.0
10	36.944942933374655	39.0	37.0	39.0	33.0	39.0
11	36.86177302800954	39.0	37.0	39.0	33.0	39.0
12	36.658513830444896	39.0	35.0	39.0	33.0	39.0
13	36.4826443533014	39.0	35.0	39.0	32.0	39.0
14	37.588264822714955	40.0	36.0	41.0	33.0	41.0
15	37.73580678536783	40.0	36.0	41.0	33.0	41.0
16	37.81805988000755	40.0	36.0	41.0	33.0	41.0
17	37.78355034953409	40.0	36.0	41.0	33.0	41.0
18	37.76633270867532	40.0	36.0	41.0	33.0	41.0
19	37.726291645885084	39.0	36.0	41.0	33.0	41.0
20	37.68034780699843	39.0	36.0	41.0	33.0	41.0
21	37.59274073141481	39.0	35.0	41.0	33.0	41.0
22	37.601452047409445	39.0	35.0	41.0	33.0	41.0
23	37.5066008758125	39.0	35.0	41.0	33.0	41.0
24	37.51408385912751	39.0	35.0	41.0	33.0	41.0
25	37.34464819809568	39.0	35.0	41.0	32.0	41.0
26	37.320573846037135	39.0	35.0	41.0	32.0	41.0
27	37.11399282369633	39.0	35.0	41.0	32.0	41.0
28	37.11469334456749	39.0	35.0	41.0	32.0	41.0
29	37.09679132387146	39.0	35.0	41.0	32.0	41.0
30	36.97416143293643	39.0	35.0	41.0	31.0	41.0
31	36.94751420007457	39.0	35.0	41.0	31.0	41.0
32	36.82769769134792	39.0	35.0	41.0	31.0	41.0
33	36.70990849647883	39.0	35.0	41.0	31.0	41.0
34	36.62624144726966	39.0	35.0	40.0	31.0	41.0
35	36.51934099386802	39.0	35.0	41.0	31.0	41.0
36	36.4513000795753	38.0	35.0	40.0	30.0	41.0
37	36.3732743217134	38.0	35.0	40.0	30.0	41.0
38	36.248329806517425	38.0	35.0	40.0	30.0	41.0
39	36.22887340425704	38.0	35.0	40.0	30.0	41.0
40	36.12133860822321	38.0	35.0	40.0	30.0	41.0
41	36.04742719990186	38.0	35.0	40.0	30.0	41.0
42	36.01668821494692	38.0	35.0	40.0	30.0	41.0
43	35.95281007329643	38.0	35.0	40.0	30.0	41.0
44	35.849381556286204	38.0	35.0	40.0	30.0	41.0
45	35.78227359375278	38.0	35.0	40.0	30.0	41.0
46	35.66288159421764	37.0	35.0	40.0	30.0	41.0
47	35.58080927915757	37.0	34.0	40.0	29.0	41.0
48	35.52210433887132	37.0	34.0	40.0	29.0	41.0
49	35.47584413572027	37.0	34.0	40.0	29.0	41.0
50	35.36578811019161	37.0	34.0	40.0	29.0	41.0
51	34.66764319921748	36.0	33.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	12.0
9	24.0
10	39.0
11	36.0
12	34.0
13	37.0
14	36.0
15	47.0
16	70.0
17	161.0
18	228.0
19	373.0
20	593.0
21	918.0
22	1383.0
23	2025.0
24	2781.0
25	3825.0
26	4859.0
27	6191.0
28	7480.0
29	8974.0
30	11458.0
31	14811.0
32	19414.0
33	27116.0
34	47287.0
35	60730.0
36	57663.0
37	79262.0
38	118224.0
39	143378.0
40	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.17161631471143	19.757109721906126	23.948936225160967	13.122337738221484
2	30.072037434285814	24.803119739031764	26.67063736100552	18.4542054656769
3	31.623675022879915	24.086619244309073	27.440080446912944	16.849625285898064
4	28.01050458486068	26.95859340574201	25.602423737650092	19.428478271747217
5	24.875108750215887	31.03985382679702	23.837885911944205	20.247151511042887
6	25.10205168681875	36.30247651883094	22.81874103163804	15.776730762712274
7	82.6477429185249	6.267079231493095	6.860907868592616	4.224269981389388
8	84.20147884152571	5.047946941193372	6.03206577794134	4.7185084393395735
9	79.13141868389238	7.012149356214863	8.399148399051553	5.457283560841207
10	43.684578371983044	24.940641347840895	15.452457391705767	15.922322888470298
11	33.33107358858764	26.48049598168961	20.981084322375185	19.207346107347558
12	29.264017277362687	22.959329436887753	27.439434805557035	20.337218480192533
13	26.268887027289644	25.39049196257217	27.69510878249795	20.64551222764023
14	20.85389297526064	28.932641851441154	27.25268304335966	22.96078212993855
15	20.5560908998465	26.515844845925763	31.54442254644179	21.383641707785948
16	22.58776283656073	23.98638342380383	30.758515605958625	22.667338133676814
17	22.709950463166965	23.19385865942257	28.205488274345925	25.890702603064536
18	25.011339076313195	24.089040399393742	27.903328119779385	22.996292404513678
19	25.349493736471796	26.75812176473152	25.370799901216873	22.521584597579814
20	27.13097964777036	24.561488461581916	25.266851642915135	23.04068024773259
21	26.12216502915878	25.72122174713779	25.126424648004402	23.03018857569903
22	23.52781665076775	24.712245718187233	25.201157634951148	26.558779996093868
23	22.592120915713135	27.596325655043508	25.298649479693776	24.51290394954958
24	23.00726830756417	25.79046678255929	27.184567880311008	24.017697029565532
25	23.78930139991187	25.962691614248655	25.928472622385357	24.319534363454117
26	22.080450141153342	27.076422953195845	26.389621960845076	24.453504944805733
27	22.854412716552147	26.363634896269645	26.490503422706237	24.29144896447197
28	21.838980274042473	27.630544646906813	27.57501949029843	22.955455588752283
29	22.645709148253783	25.725902646968144	27.04075126828174	24.58763693649633
30	24.285799602607746	25.612753999344672	26.610915535583718	23.490530862463864
31	25.400176582910845	26.936964420318983	22.934310834346185	24.728548162423998
32	25.538021012397927	26.761188561172094	24.805379483777454	22.895410942652518
33	25.385649652402837	25.967372514079017	23.737165860422024	24.909811973096126
34	23.310235513825603	26.291323064407568	26.602845018634824	23.795596403132006
35	23.964915848719773	24.75663356140614	25.765609590356704	25.512840999517383
36	25.820811926287124	26.12942849441278	25.087847576988697	22.961912002311397
37	25.491696245111285	26.657885944226273	25.68974673103711	22.160671079625335
38	24.587798346835307	27.55887845640065	25.242155861051526	22.61116733571252
39	25.93331493255469	25.013921641736843	25.837437191201847	23.215326234506627
40	26.28874049898392	24.105504253969485	27.74885842537758	21.856896821669014
41	22.87378195722949	23.64580760856056	27.77500690029199	25.705403533917963
42	24.06741141397071	24.187662116509212	26.39785388813295	25.347072581387124
43	24.403951970739534	23.533466012631973	26.497928298299218	25.56465371832927
44	23.884049268891868	24.84928309597943	26.080521161702492	25.186146473426206
45	24.82604000716662	24.248836634981817	26.35895399643929	24.56616936141228
46	24.663338385476944	24.7343589346272	27.074163208450152	23.5281394714457
47	23.510061513480185	25.266367411898198	27.814552433341568	23.40901864128005
48	23.963301745329996	24.14343568362928	27.717544819615874	24.17571775142485
49	24.743397913609957	23.034869475529383	28.036330239097136	24.18540237176352
50	22.6098760530007	23.749755866862294	29.33406936447907	24.306298715657935
51	22.21958585335225	23.907937999060593	26.325703466609852	27.546772680977305
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1219.0
1	1340.0
2	1461.0
3	1879.5
4	2298.0
5	1725.0
6	1152.0
7	1127.5
8	1103.0
9	1130.5
10	1158.0
11	1205.0
12	1252.0
13	1222.5
14	1193.0
15	1321.0
16	1449.0
17	1469.0
18	1489.0
19	1605.5
20	1722.0
21	1883.5
22	2045.0
23	2128.0
24	2211.0
25	2547.5
26	3767.5
27	4651.0
28	5144.5
29	5638.0
30	6069.0
31	6500.0
32	8233.5
33	9967.0
34	11275.5
35	12584.0
36	13821.5
37	15059.0
38	16351.0
39	17643.0
40	20255.0
41	22867.0
42	27364.0
43	31861.0
44	34695.5
45	37530.0
46	41525.0
47	45520.0
48	49905.5
49	54291.0
50	53702.5
51	53114.0
52	49114.0
53	45114.0
54	41436.0
55	37758.0
56	35131.0
57	32504.0
58	30898.0
59	29292.0
60	30042.5
61	30793.0
62	28452.0
63	26111.0
64	23824.5
65	21538.0
66	18206.0
67	14874.0
68	13095.5
69	11317.0
70	10148.5
71	8980.0
72	8386.0
73	7792.0
74	6877.5
75	4548.0
76	3133.0
77	2610.0
78	2087.0
79	1738.0
80	1389.0
81	1051.5
82	714.0
83	472.0
84	230.0
85	134.5
86	39.0
87	34.0
88	29.0
89	19.0
90	9.0
91	7.5
92	6.0
93	5.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	619539.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.902360511383964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.68219122039395	18.846652100617483
2	9.595127876599694	4.778826670718313
3	3.4150890060483814	2.551313328212422
4	1.6473786890980457	1.6409447205876262
5	0.9480976065449876	1.1804934199081776
6	0.6450901672281194	0.9638560743998157
7	0.4784542353418092	0.8340247899676195
8	0.37641264980332334	0.7498850805158144
9	0.3284857942120344	0.7362064503302728
>10	4.952274064922422	33.0451627505106
>50	1.8289216386751936	29.95759582217114
>100	0.09660257049390744	3.484590513796105
>500	0.00456904049633346	0.8065369031372456
>1k	0.00130544014180956	0.4239113751273552
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	1354	0.2185495989760128	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	1254	0.20240856507822755	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	947	0.1528555910120267	No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	935	0.15091866694429246	TruSeq Adapter, Index 15 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	905	0.14607635677495687	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6141033897785288E-4	0.0	0.0	0.15915059423216293	0.0
2	1.6141033897785288E-4	0.0	0.0	0.740550635230389	0.0
3	1.6141033897785288E-4	0.0	0.0	0.9823433230192127	0.0
4	1.6141033897785288E-4	0.0	0.0	1.2522214097901827	0.0
5	1.6141033897785288E-4	0.0	0.0	2.017629237223161	0.0
6	1.6141033897785288E-4	0.0	0.0	2.6070997951702797	0.0
7	1.6141033897785288E-4	0.0	0.0	3.1349116036278586	0.0
8	1.6141033897785288E-4	0.0	0.0	4.058985794276067	0.0
9	1.6141033897785288E-4	0.0	0.0	4.493018195787513	0.0
10	1.6141033897785288E-4	0.0	0.0	5.436623037452041	0.0
11	1.6141033897785288E-4	0.0	0.0	6.602328505550094	0.0
12	1.6141033897785288E-4	0.0	0.0	7.516233844842698	0.0
13	1.6141033897785288E-4	0.0	0.0	7.880214159237756	0.0
14	1.6141033897785288E-4	0.0	0.0	8.028873081436359	0.0
15	1.6141033897785288E-4	0.0	0.0	8.294554499393904	0.0
16	1.6141033897785288E-4	0.0	0.0	8.844963755308383	0.0
17	1.6141033897785288E-4	0.0	0.0	9.526599616811856	0.0
18	1.6141033897785288E-4	0.0	0.0	10.350599397293795	0.0
19	1.6141033897785288E-4	0.0	0.0	10.737661390162685	0.0
20	1.6141033897785288E-4	0.0	0.0	11.109227990489703	0.0
21	1.6141033897785288E-4	0.0	0.0	11.569893097932495	0.0
22	1.6141033897785288E-4	0.0	0.0	12.030558205375288	0.0
23	1.6141033897785288E-4	0.0	0.0	12.512690887902133	0.0
24	1.6141033897785288E-4	0.0	0.0	12.88135210212755	0.0
25	1.6141033897785288E-4	0.0	0.0	13.189323028897293	0.0
26	1.6141033897785288E-4	0.0	0.0	13.476310611599915	0.0
27	1.6141033897785288E-4	0.0	0.0	13.780569100573167	0.0
28	1.6141033897785288E-4	0.0	0.0	14.102098495817051	0.0
29	1.6141033897785288E-4	0.0	0.0	14.411199294959639	0.0
30	1.6141033897785288E-4	0.0	0.0	14.75242075155882	0.0
31	1.6141033897785288E-4	0.0	0.0	15.06894642629439	0.0
32	1.6141033897785288E-4	0.0	0.0	15.405164162385258	0.0
33	1.6141033897785288E-4	0.0	0.0	15.721689837120827	0.0
34	1.6141033897785288E-4	0.0	0.0	16.03418025338195	0.0
35	1.6141033897785288E-4	0.0	0.0	16.37185068252362	0.0
36	1.6141033897785288E-4	0.0	0.0	16.677561864547673	0.0
37	1.6141033897785288E-4	0.0	0.0	16.994248949622218	0.0
38	1.6141033897785288E-4	0.0	0.0	17.31158167605268	0.0
39	3.2282067795570576E-4	0.0	0.0	17.61987542350038	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTT	20	7.030765E-4	45.000004	45
ACCGGTT	20	7.030765E-4	45.000004	38
GTCGATC	20	7.030765E-4	45.000004	35
CGTATGA	20	7.030765E-4	45.000004	30
GTCGAAA	20	7.030765E-4	45.000004	9
GCGAACC	20	7.030765E-4	45.000004	23
TAGGTCG	20	7.030765E-4	45.000004	1
TGGCGAA	20	7.030765E-4	45.000004	23
GGCCGAT	80	0.0	45.000004	8
CGCGAAT	20	7.030765E-4	45.000004	35
AATATCG	20	7.030765E-4	45.000004	1
ATCCGTG	20	7.030765E-4	45.000004	1
CCCAATC	20	7.030765E-4	45.000004	17
GTTCGAC	20	7.030765E-4	45.000004	33
AAATTCG	20	7.030765E-4	45.000004	30
CTCGCAC	20	7.030765E-4	45.000004	39
TATAGCG	20	7.030765E-4	45.000004	1
TTACGGT	20	7.030765E-4	45.000004	33
TGTAGCG	40	6.8066583E-9	45.000004	1
CGGTCGA	20	7.030765E-4	45.000004	33
>>END_MODULE