FastQCFastQC Report
Sat 18 Jun 2016
SRR3552572_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552572_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1057945
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC39010.3687337243429479No Hit
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC38590.36476376371172414No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG33870.3201489680465431No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC24980.23611813468564055TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC20160.19055811029873954TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15330.14490356303966653No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT14210.1343170013564032TruSeq Adapter, Index 15 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCC11340.107188937043041No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCG406.8139343E-945.01
CGTATCA207.0330314E-445.034
CCGATCG253.8905957E-545.040
TTAGCGA207.0330314E-445.014
CGTTGCG253.8905957E-545.017
TTCGTAG253.8905957E-545.01
TAAGTCG207.0330314E-445.01
TCTAGCG253.8905957E-545.01
ACGATCG253.8905957E-545.040
CGCTCGT253.8905957E-545.013
TACGCAA207.0330314E-445.036
ACTACGC253.8905957E-545.013
TTACGTC207.0330314E-445.024
CGTAACG207.0330314E-445.010
CCGAAAC207.0330314E-445.022
TACGTCG207.0330314E-445.01
CTTACCG253.8905957E-545.01
TAGCACG302.1653377E-644.9999961
CTACGCA700.041.78571315
TGTAGCG700.041.7857131