##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3552572_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1057945 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79344389358615 31.0 31.0 34.0 30.0 34.0 2 31.936217856315782 33.0 31.0 34.0 30.0 34.0 3 31.970682785967135 33.0 31.0 34.0 30.0 34.0 4 35.60747392350264 37.0 35.0 37.0 33.0 37.0 5 35.422256355481615 37.0 35.0 37.0 33.0 37.0 6 35.442913383966086 37.0 35.0 37.0 33.0 37.0 7 35.870165273241994 37.0 35.0 37.0 35.0 37.0 8 35.69835955555345 37.0 35.0 37.0 35.0 37.0 9 37.4676849930762 39.0 37.0 39.0 35.0 39.0 10 36.85332602356455 39.0 37.0 39.0 32.0 39.0 11 36.79333897319804 39.0 37.0 39.0 32.0 39.0 12 36.7796898704564 39.0 35.0 39.0 33.0 39.0 13 36.63417852534867 39.0 35.0 39.0 32.0 39.0 14 37.78743885551707 40.0 37.0 41.0 33.0 41.0 15 37.90545727802485 40.0 37.0 41.0 33.0 41.0 16 37.9669907225801 40.0 37.0 41.0 33.0 41.0 17 37.92204604209103 40.0 37.0 41.0 33.0 41.0 18 37.899896497454975 40.0 37.0 41.0 33.0 41.0 19 37.85742548053065 40.0 37.0 41.0 33.0 41.0 20 37.76009716951259 40.0 36.0 41.0 33.0 41.0 21 37.75188502237829 40.0 36.0 41.0 33.0 41.0 22 37.80088946022714 40.0 36.0 41.0 33.0 41.0 23 37.677987040914225 39.0 36.0 41.0 33.0 41.0 24 37.71528387581585 40.0 36.0 41.0 33.0 41.0 25 37.60463256596515 39.0 36.0 41.0 33.0 41.0 26 37.603180694648586 39.0 36.0 41.0 33.0 41.0 27 37.347040725179475 39.0 36.0 41.0 32.0 41.0 28 37.41422852794805 39.0 36.0 41.0 32.0 41.0 29 37.43091370534385 39.0 36.0 41.0 32.0 41.0 30 37.31472808132748 39.0 36.0 41.0 32.0 41.0 31 37.31609299160164 39.0 36.0 41.0 32.0 41.0 32 37.17178492265666 39.0 36.0 41.0 31.0 41.0 33 37.04938441979498 39.0 35.0 41.0 31.0 41.0 34 37.0337531724239 39.0 35.0 41.0 31.0 41.0 35 36.87110388536266 39.0 35.0 41.0 31.0 41.0 36 36.84020435844963 39.0 35.0 41.0 31.0 41.0 37 36.81528056751532 39.0 35.0 41.0 31.0 41.0 38 36.77000411174494 39.0 35.0 41.0 31.0 41.0 39 36.74328627669681 39.0 35.0 41.0 31.0 41.0 40 36.67405583466059 39.0 35.0 41.0 30.0 41.0 41 36.60431969525826 39.0 35.0 41.0 30.0 41.0 42 36.586453927189034 39.0 35.0 41.0 30.0 41.0 43 36.55491637088885 39.0 35.0 41.0 30.0 41.0 44 36.560614209623374 39.0 35.0 41.0 30.0 41.0 45 36.52455940526209 39.0 35.0 41.0 30.0 41.0 46 36.419880995703934 39.0 35.0 40.0 30.0 41.0 47 36.32922694468994 39.0 35.0 40.0 30.0 41.0 48 36.277436917798184 39.0 35.0 40.0 30.0 41.0 49 36.25241765876298 39.0 35.0 40.0 30.0 41.0 50 36.19018474495366 38.0 35.0 40.0 30.0 41.0 51 35.520354082679155 38.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 5.0 9 17.0 10 32.0 11 40.0 12 30.0 13 27.0 14 32.0 15 53.0 16 92.0 17 133.0 18 250.0 19 490.0 20 897.0 21 1494.0 22 2601.0 23 3788.0 24 5007.0 25 6667.0 26 8043.0 27 9188.0 28 10708.0 29 13606.0 30 17590.0 31 22981.0 32 30809.0 33 41493.0 34 64855.0 35 83840.0 36 91142.0 37 131224.0 38 217440.0 39 293227.0 40 144.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.7510976468531 18.882267036566173 24.738148013365535 11.628487303215195 2 31.333859510655092 26.16156794540359 25.00167778098105 17.502894762960267 3 31.50551304651943 26.316774501510004 25.998326945162553 16.17938550680801 4 26.75999224912448 26.713203427399346 27.990774567676013 18.53602975580016 5 26.274239209032608 30.49799375203815 24.480478663824677 18.747288375104564 6 23.604346161662466 38.05850020558725 23.73015610452339 14.606997528226891 7 82.4202581419639 5.338368251657695 8.617083118687646 3.6242904876907587 8 84.0136301981672 5.4155934382222135 6.480015501751037 4.090760861859549 9 78.3829972257537 6.764340301244394 8.20912240239332 6.643540070608586 10 37.27150277188323 29.01899437116296 18.86128295894399 14.848219898009821 11 28.61736668730416 27.243193171667713 24.848267159445907 19.291172981582218 12 23.888009301050623 24.421401868717183 30.528808208366222 21.161780621865976 13 24.151917160154827 26.38256242054171 31.790499506117992 17.67502091318547 14 20.96384972753782 30.070372278332048 29.3668385407559 19.598939453374232 15 20.139799327942377 27.89691335560922 33.01976945871477 18.943517857733625 16 22.414397723889238 26.29881515579732 30.590437121022358 20.696349999291076 17 23.112638180623758 26.268284268085768 28.98392638558715 21.63515116570332 18 23.708510366796006 26.11931622154271 29.653337366309213 20.518836045352074 19 22.53633222899111 28.531634442244165 28.29589439904721 20.636138929717518 20 23.8367779043334 27.86647699076984 28.83561999914929 19.461125105747463 21 22.704015804224227 28.948102217033966 29.008313286607528 19.33956869213428 22 21.3881629007179 26.2563743861921 28.73268459135399 23.622778121736008 23 20.601259989886053 28.81822779066965 28.972961732415204 21.607550487029098 24 20.567609847392823 27.679699795357983 29.677251652968728 22.07543870428047 25 20.064370075949128 29.90297227171545 27.706449768182654 22.32620788415277 26 20.30474173988251 29.05453497109963 29.471569883122466 21.16915340589539 27 21.88875603174078 28.769453988628896 28.506113266757723 20.835676712872598 28 19.540524318371936 28.59600451819329 29.955337942898737 21.908133220536037 29 20.679241359427948 26.144459305540458 30.145990576069643 23.03030875896195 30 21.622201532215758 29.444158250192594 28.171596822141037 20.762043395450615 31 21.9612550746967 28.225947473639934 26.157219893283678 23.65557755837969 32 21.185222294164628 28.707636030228418 27.546328022723298 22.560813652883656 33 21.270765493480283 29.096219557727483 25.942463927708907 23.690551021083326 34 21.019051084886264 27.19451389249914 28.135772653587853 23.650662369026744 35 20.828776543204043 27.243098648795545 28.77711034127483 23.151014466725588 36 21.26292009509001 29.812513883046847 27.369759297506015 21.554806724357125 37 20.47261436085997 29.332337692413123 27.64028375766226 22.55476418906465 38 20.11758645298196 28.94640080533487 26.908582204178856 24.027430537504312 39 22.18206050409048 28.38966108824183 26.68891104925114 22.739367358416555 40 22.234048083785073 27.223815982872456 28.78854760880764 21.753588324534828 41 19.724276781874295 28.314704450609433 28.542599095416115 23.418419672100157 42 21.12680715916234 26.926447027019364 29.616946060523 22.329799753295305 43 20.784256270411035 27.67932170386929 28.435221112628728 23.101200913090945 44 20.743895003993593 27.592360661471062 27.466456195737965 24.197288138797386 45 21.26953669614205 26.67974233065046 27.374485441114615 24.67623553209288 46 22.28319997731451 27.977730411316276 27.263326543440346 22.475743067928864 47 19.912188251752216 27.908823237502894 30.018290175765284 22.160698334979607 48 20.58235541545165 27.293857431151903 28.77474726947053 23.34903988392591 49 21.466900453237173 25.66579548086148 29.896072102046894 22.971231963854454 50 20.32714366058727 26.30647150844326 29.08185208115734 24.284532749812136 51 20.231864605437902 25.365212747354544 27.38384320545964 27.019079441747916 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1299.0 1 1427.5 2 1556.0 3 2482.0 4 3408.0 5 2538.5 6 1669.0 7 1625.5 8 1582.0 9 1759.5 10 1937.0 11 1931.5 12 1926.0 13 2033.5 14 2141.0 15 2148.0 16 2155.0 17 2279.0 18 2403.0 19 2519.5 20 2636.0 21 3144.5 22 3653.0 23 4128.5 24 4604.0 25 5396.0 26 7893.0 27 9598.0 28 11420.5 29 13243.0 30 15206.5 31 17170.0 32 20197.5 33 23225.0 34 27059.0 35 30893.0 36 34337.0 37 37781.0 38 41432.0 39 45083.0 40 51520.0 41 57957.0 42 65793.5 43 73630.0 44 87699.5 45 101769.0 46 106783.0 47 111797.0 48 110904.0 49 110011.0 50 101433.5 51 92856.0 52 84080.5 53 75305.0 54 67562.5 55 59820.0 56 52638.5 57 45457.0 58 40564.5 59 35672.0 60 31370.5 61 27069.0 62 23131.0 63 19193.0 64 16031.0 65 12869.0 66 10297.0 67 7725.0 68 6253.0 69 4781.0 70 3915.5 71 3050.0 72 2382.5 73 1715.0 74 1427.0 75 980.0 76 821.0 77 738.5 78 656.0 79 469.0 80 282.0 81 183.5 82 85.0 83 94.5 84 104.0 85 65.0 86 26.0 87 14.5 88 3.0 89 3.0 90 3.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1057945.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.07482220810301 #Duplication Level Percentage of deduplicated Percentage of total 1 74.22306026435501 21.58022280927437 2 10.3016143802716 5.990352131256682 3 3.808412242391602 3.3218672652809618 4 1.8485411497764301 2.149840210964481 5 1.110520464446443 1.6144092531120158 6 0.7727620131051975 1.3480750884125632 7 0.570039900900385 1.160166613914235 8 0.5081306368433415 1.1819046339768249 9 0.40527466329097694 1.060495990257057 >10 5.474736327216764 38.17482402104834 >50 0.906801257734572 16.812407454308424 >100 0.06453747586232961 3.07160667230676 >500 0.0029484126028447057 0.6527449927221954 >1k 0.0026208112025286273 1.8810828631651302 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC 3901 0.3687337243429479 No Hit GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC 3859 0.36476376371172414 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG 3387 0.3201489680465431 No Hit GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC 2498 0.23611813468564055 TruSeq Adapter, Index 21 (95% over 21bp) CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC 2016 0.19055811029873954 TruSeq Adapter, Index 21 (95% over 21bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1533 0.14490356303966653 No Hit CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT 1421 0.1343170013564032 TruSeq Adapter, Index 15 (95% over 24bp) CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCC 1134 0.107188937043041 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.14424190293446257 0.0 2 0.0 0.0 0.0 0.7496608991960829 0.0 3 0.0 0.0 0.0 1.0223593854122852 0.0 4 0.0 0.0 0.0 1.453383682516577 0.0 5 0.0 0.0 0.0 2.782564310999154 0.0 6 0.0 0.0 0.0 3.5691836532144867 0.0 7 0.0 0.0 0.0 4.33784364971714 0.0 8 0.0 0.0 0.0 5.574297340598991 0.0 9 0.0 0.0 0.0 6.076308314704451 0.0 10 0.0 0.0 0.0 7.518349252560388 0.0 11 0.0 0.0 0.0 8.95055981171044 0.0 12 0.0 0.0 0.0 10.431922264389925 0.0 13 0.0 0.0 0.0 10.903496873656003 0.0 14 0.0 0.0 0.0 11.08866718024094 0.0 15 0.0 0.0 0.0 11.443978656735464 0.0 16 0.0 0.0 0.0 12.111499180014084 0.0 17 0.0 0.0 0.0 12.957856977442116 0.0 18 9.452287217199382E-5 0.0 0.0 13.834934708326047 0.0 19 9.452287217199382E-5 0.0 0.0 14.461148736465507 0.0 20 9.452287217199382E-5 0.0 0.0 14.996715330192023 0.0 21 9.452287217199382E-5 0.0 0.0 15.598731503055452 0.0 22 9.452287217199382E-5 0.0 0.0 16.2661575034619 0.0 23 9.452287217199382E-5 0.0 0.0 16.95173189532537 0.0 24 9.452287217199382E-5 0.0 0.0 17.468204868873144 0.0 25 9.452287217199382E-5 0.0 0.0 17.92692436752383 0.0 26 9.452287217199382E-5 0.0 0.0 18.38337531724239 0.0 27 9.452287217199382E-5 0.0 0.0 18.837746763773165 0.0 28 9.452287217199382E-5 0.0 0.0 19.30298834060372 0.0 29 9.452287217199382E-5 0.0 0.0 19.768135394562098 0.0 30 9.452287217199382E-5 0.0 0.0 20.29774704734178 0.0 31 9.452287217199382E-5 0.0 0.0 20.79418117198909 0.0 32 1.8904574434398764E-4 0.0 0.0 21.27293951954024 0.0 33 1.8904574434398764E-4 0.0 0.0 21.741489396896814 0.0 34 1.8904574434398764E-4 0.0 0.0 22.209661182764698 0.0 35 1.8904574434398764E-4 0.0 0.0 22.69049903350363 0.0 36 1.8904574434398764E-4 0.0 0.0 23.152432309808166 0.0 37 1.8904574434398764E-4 0.0 0.0 23.62589737651768 0.0 38 1.8904574434398764E-4 0.0 0.0 24.10276526662539 0.0 39 1.8904574434398764E-4 0.0 0.0 24.576608424823597 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGCGCG 40 6.8139343E-9 45.0 1 CGTATCA 20 7.0330314E-4 45.0 34 CCGATCG 25 3.8905957E-5 45.0 40 TTAGCGA 20 7.0330314E-4 45.0 14 CGTTGCG 25 3.8905957E-5 45.0 17 TTCGTAG 25 3.8905957E-5 45.0 1 TAAGTCG 20 7.0330314E-4 45.0 1 TCTAGCG 25 3.8905957E-5 45.0 1 ACGATCG 25 3.8905957E-5 45.0 40 CGCTCGT 25 3.8905957E-5 45.0 13 TACGCAA 20 7.0330314E-4 45.0 36 ACTACGC 25 3.8905957E-5 45.0 13 TTACGTC 20 7.0330314E-4 45.0 24 CGTAACG 20 7.0330314E-4 45.0 10 CCGAAAC 20 7.0330314E-4 45.0 22 TACGTCG 20 7.0330314E-4 45.0 1 CTTACCG 25 3.8905957E-5 45.0 1 TAGCACG 30 2.1653377E-6 44.999996 1 CTACGCA 70 0.0 41.785713 15 TGTAGCG 70 0.0 41.785713 1 >>END_MODULE