FastQCFastQC Report
Sat 18 Jun 2016
SRR3552571_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552571_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738170
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17460.23653088042049933No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC17320.23463429833236246TruSeq Adapter, Index 22 (95% over 24bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC17280.23409241773575193No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14130.19141932075267215No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT12310.16676375360689272Illumina Single End Adapter 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC9890.13397997751195523TruSeq Adapter, Index 22 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCC7730.10471842529498625No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATT207.0316443E-445.015
CGACCGT207.0316443E-445.024
TCGATTG207.0316443E-445.01
ATCCGTA207.0316443E-445.034
ACGATTG207.0316443E-445.011
CCACTAG253.8894446E-545.018
ATTTTCG253.8894446E-545.027
CTACGTT207.0316443E-445.042
GTATGAT253.8894446E-545.035
CGTAGAA253.8894446E-545.042
ACTACGT207.0316443E-445.041
AGCGATC253.8894446E-545.045
TACCTAC453.8380676E-1045.024
GAACGGT207.0316443E-445.021
CATACCG207.0316443E-445.01
TCCGTAT253.8894446E-545.015
TTACGCC207.0316443E-445.013
TTACGAC207.0316443E-445.013
ATAACGC207.0316443E-445.011
CGACTCA253.8894446E-545.025