##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552571_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	738170
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81146619342428	31.0	31.0	34.0	30.0	34.0
2	31.955003589958952	33.0	31.0	34.0	30.0	34.0
3	31.988766815232264	33.0	31.0	34.0	30.0	34.0
4	35.62216968990883	37.0	35.0	37.0	33.0	37.0
5	35.44694311608437	37.0	35.0	37.0	33.0	37.0
6	35.4575219800317	37.0	35.0	37.0	33.0	37.0
7	35.830899386320226	37.0	35.0	37.0	35.0	37.0
8	35.6569787447336	37.0	35.0	37.0	35.0	37.0
9	37.399520435672	39.0	37.0	39.0	35.0	39.0
10	36.928857851172495	39.0	37.0	39.0	33.0	39.0
11	36.84045951474592	39.0	37.0	39.0	33.0	39.0
12	36.62180933931208	39.0	35.0	39.0	32.0	39.0
13	36.39381578769118	39.0	35.0	39.0	32.0	39.0
14	37.47847921210561	39.0	36.0	41.0	32.0	41.0
15	37.669439289052654	39.0	36.0	41.0	33.0	41.0
16	37.741449801536234	40.0	36.0	41.0	33.0	41.0
17	37.71535147730198	39.0	36.0	41.0	33.0	41.0
18	37.69477898045166	39.0	36.0	41.0	33.0	41.0
19	37.64667488518905	39.0	36.0	41.0	33.0	41.0
20	37.58219922240134	39.0	35.0	41.0	33.0	41.0
21	37.4756831082271	39.0	35.0	41.0	33.0	41.0
22	37.4926778384383	39.0	35.0	41.0	33.0	41.0
23	37.39548477992874	39.0	35.0	41.0	33.0	41.0
24	37.41875719685167	39.0	35.0	41.0	33.0	41.0
25	37.26386740181801	39.0	35.0	41.0	32.0	41.0
26	37.238516872807075	39.0	35.0	41.0	32.0	41.0
27	37.02833358169527	39.0	35.0	41.0	32.0	41.0
28	37.049541433545116	39.0	35.0	41.0	32.0	41.0
29	37.04280585772925	39.0	35.0	41.0	32.0	41.0
30	36.92325480580354	39.0	35.0	41.0	31.0	41.0
31	36.860507742119026	39.0	35.0	41.0	31.0	41.0
32	36.74453716623542	39.0	35.0	40.0	31.0	41.0
33	36.625742037741986	39.0	35.0	40.0	31.0	41.0
34	36.505059810070854	39.0	35.0	40.0	31.0	41.0
35	36.39057805112643	38.0	35.0	40.0	30.0	41.0
36	36.31509408401859	38.0	35.0	40.0	30.0	41.0
37	36.26744381375564	38.0	35.0	40.0	30.0	41.0
38	36.17821910941924	38.0	35.0	40.0	30.0	41.0
39	36.14068439519352	38.0	35.0	40.0	30.0	41.0
40	36.03176774997629	38.0	35.0	40.0	30.0	41.0
41	35.93407345191487	38.0	35.0	40.0	30.0	41.0
42	35.9197271631196	38.0	35.0	40.0	30.0	41.0
43	35.85823319831475	38.0	35.0	40.0	30.0	41.0
44	35.775195415690156	38.0	35.0	40.0	30.0	41.0
45	35.711800804692686	38.0	35.0	40.0	30.0	41.0
46	35.56985111830608	37.0	34.0	40.0	29.0	41.0
47	35.49512578403349	37.0	34.0	40.0	29.0	41.0
48	35.43555820474958	37.0	34.0	40.0	29.0	41.0
49	35.39409756560142	37.0	34.0	40.0	29.0	41.0
50	35.309323055664684	37.0	34.0	40.0	29.0	41.0
51	34.61572808431662	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	22.0
10	48.0
11	45.0
12	40.0
13	24.0
14	39.0
15	61.0
16	85.0
17	156.0
18	294.0
19	476.0
20	758.0
21	1194.0
22	1866.0
23	2557.0
24	3598.0
25	4833.0
26	6193.0
27	7886.0
28	9246.0
29	11211.0
30	13814.0
31	17832.0
32	23734.0
33	33223.0
34	57561.0
35	74462.0
36	66430.0
37	93236.0
38	139374.0
39	167766.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.205670780443526	18.389530866873486	24.465637996667432	12.939160356015552
2	31.972851782109814	24.16232033271469	25.627429995800426	18.237397889375075
3	31.841445737431755	25.031903220125447	26.236910196838124	16.88974084560467
4	28.989121747023045	27.378517143747377	25.10830838424753	18.524052724982052
5	25.03000663803731	30.63874175325467	23.83339881057209	20.49785279813593
6	25.16019345137299	35.39103458552908	24.282482355013073	15.16628960808486
7	81.6093853719333	6.538195808553585	7.473752658601677	4.378666160911443
8	82.43453405042199	5.525420973488491	7.0911849573946375	4.94886001869488
9	77.91579175528672	7.42552528550334	9.39471937358603	5.263963585623908
10	42.82739748296463	25.382635436281614	16.06838533129225	15.721581749461505
11	33.518837124239674	26.689651435306228	21.106926588726175	18.684584851727923
12	29.49794762724034	23.162415161819094	28.31231288185648	19.027324329084085
13	24.356449056450412	26.88716691277077	29.16591029166723	19.590473739111587
14	20.894644865003997	29.287697955755448	28.946313179890808	20.871343999349744
15	20.473061760841	26.14004904019399	33.50691575111424	19.879973447850766
16	22.701410244252678	23.817006922524623	31.9954752970183	21.486107536204397
17	22.02839454326239	24.47159868323015	27.609493748052614	25.890513025454844
18	23.781649213595784	24.87286126502025	28.244984217727627	23.10050530365634
19	25.310158906484958	27.028733218635274	25.540864570491895	22.12024330438788
20	26.59455139060108	25.459717951149464	26.201958898356747	21.74377175989271
21	25.2685695706951	26.824715174011406	27.177479442404866	20.72923581288863
22	23.759567579283907	25.151252421528913	25.31503583185445	25.77414416733273
23	23.151171139439423	27.384206890011786	25.76750613002425	23.69711584052454
24	22.528550333933918	25.12090710811873	28.642047224893997	23.70849533305336
25	22.839589796388367	26.3616782042077	26.98389259926575	23.81483940013818
26	21.61521058834686	28.042456344744437	26.521803920506116	23.820529146402592
27	23.024100139534255	27.454922307869463	26.808729696411394	22.712247856184888
28	20.47509381307829	27.614506143571266	28.861102456073805	23.049297587276644
29	22.573661893601745	25.901621577685358	27.56329842719157	23.96141810152133
30	23.841256079222944	27.033339203706465	26.92103445005893	22.204370267011665
31	25.55373423466139	27.826923337442594	24.003413847758644	22.615928580137368
32	25.575138518227508	28.10084397902922	23.87187233293144	22.452145169811832
33	25.823455301624286	27.793462210601895	22.44225584892369	23.94082663885013
34	22.627714483113646	27.11191189021499	26.49606459216712	23.764309034504247
35	24.01533522088408	25.086497690233955	26.064185756668518	24.833981332213448
36	26.501212457834917	27.61179674058821	24.189414362545214	21.69757643903166
37	24.350217429589392	27.767316471815434	25.66929027188859	22.213175826706586
38	24.908896324694854	27.75986561361204	23.876342847853476	23.45489521383963
39	24.65732825771841	25.621333839088557	25.85407155533278	23.86726634786025
40	25.355405936301935	24.140645108850265	27.625885636100083	22.878063318747714
41	22.624734139832288	24.96904507091862	26.242464472953387	26.163756316295704
42	24.114499370063808	25.04694040668139	26.28595039083138	24.552609832423425
43	23.44676700489047	23.756993646450006	26.76659848002493	26.029640868634594
44	23.04347237086308	25.07958871262717	26.439031659373857	25.43790725713589
45	24.2838370565046	24.59094788463362	25.967731010471844	25.15748404838994
46	25.209098175217093	25.975588279122697	26.034517794004092	22.78079575165612
47	22.964493273907095	25.18959047373911	27.940040911985044	23.90587534036875
48	23.586030318219382	24.670739802484523	27.32676754677107	24.416462332525025
49	23.63791538534484	24.172887004348592	27.925003725429104	24.264193884877468
50	22.267634826665944	24.584038907026837	28.31583510573445	24.83249116057277
51	21.910941923947057	24.055298914884105	26.101981928282104	27.931777232886734
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1747.0
1	1805.0
2	1863.0
3	2814.0
4	3765.0
5	2711.5
6	1658.0
7	1732.0
8	1806.0
9	1889.0
10	1972.0
11	2050.5
12	2129.0
13	2069.5
14	2010.0
15	2088.0
16	2166.0
17	2104.0
18	2042.0
19	1946.5
20	1851.0
21	2118.5
22	2386.0
23	2410.5
24	2435.0
25	3339.0
26	4980.0
27	5717.0
28	6223.5
29	6730.0
30	7687.5
31	8645.0
32	10249.5
33	11854.0
34	13890.5
35	15927.0
36	17442.5
37	18958.0
38	19534.5
39	20111.0
40	23487.5
41	26864.0
42	31652.0
43	36440.0
44	40940.0
45	45440.0
46	48512.5
47	51585.0
48	54774.5
49	57964.0
50	61264.5
51	64565.0
52	65909.0
53	67253.0
54	58840.5
55	50428.0
56	48006.0
57	45584.0
58	40936.0
59	36288.0
60	34220.0
61	32152.0
62	28936.0
63	25720.0
64	24276.5
65	22833.0
66	19085.5
67	15338.0
68	13615.5
69	11893.0
70	10743.0
71	9593.0
72	7939.0
73	6285.0
74	5207.5
75	3885.5
76	3641.0
77	2799.5
78	1958.0
79	1603.0
80	1248.0
81	880.0
82	512.0
83	365.5
84	219.0
85	157.0
86	95.0
87	66.0
88	37.0
89	31.5
90	26.0
91	41.5
92	57.0
93	31.5
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	738170.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.243147071874553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.76005361902222	18.124189749871828
2	10.51304717216372	5.097386975366398
3	3.9144648139571325	2.8469683857732244
4	1.9798095529717261	1.9198725666798306
5	1.1521865572020957	1.3966314080243605
6	0.7441767734836989	1.082471218022303
7	0.5041846181169796	0.855611529387079
8	0.35456618361272174	0.6876640108829192
9	0.28289144564000673	0.6172361029823299
>10	3.501185295323675	21.612548228344195
>50	1.9558332861940315	33.65126609703678
>100	0.33084866866573354	10.363626914701833
>500	0.003938674626973018	0.673721004695128
>1k	0.0028133390192664415	1.0708058082317786
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1746	0.23653088042049933	No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	1732	0.23463429833236246	TruSeq Adapter, Index 22 (95% over 24bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1728	0.23409241773575193	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1413	0.19141932075267215	No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	1231	0.16676375360689272	Illumina Single End Adapter 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	989	0.13397997751195523	TruSeq Adapter, Index 22 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCC	773	0.10471842529498625	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7094029830526846E-4	0.0	0.0	0.1849167535933457	0.0
2	2.7094029830526846E-4	0.0	0.0	0.7566007830174621	0.0
3	2.7094029830526846E-4	0.0	0.0	1.0246962081905253	0.0
4	2.7094029830526846E-4	0.0	0.0	1.346302342278879	0.0
5	2.7094029830526846E-4	0.0	0.0	2.2093826625303112	0.0
6	2.7094029830526846E-4	0.0	0.0	2.8897137515748406	0.0
7	2.7094029830526846E-4	0.0	0.0	3.5375320048227374	0.0
8	2.7094029830526846E-4	0.0	0.0	4.642155601013317	0.0
9	2.7094029830526846E-4	0.0	0.0	5.146240026010268	0.0
10	2.7094029830526846E-4	0.0	0.0	6.207513174472005	0.0
11	2.7094029830526846E-4	0.0	0.0	7.461695815327093	0.0
12	2.7094029830526846E-4	0.0	0.0	8.439112941463348	0.0
13	2.7094029830526846E-4	0.0	0.0	8.807727217307667	0.0
14	2.7094029830526846E-4	0.0	0.0	8.949970873917932	0.0
15	2.7094029830526846E-4	0.0	0.0	9.19828765731471	0.0
16	2.7094029830526846E-4	0.0	0.0	9.763875530026958	0.0
17	2.7094029830526846E-4	0.0	0.0	10.45409593995963	0.0
18	2.7094029830526846E-4	0.0	0.0	11.333161737811073	0.0
19	2.7094029830526846E-4	0.0	0.0	11.761518349431702	0.0
20	2.7094029830526846E-4	0.0	0.0	12.209111722232006	0.0
21	2.7094029830526846E-4	0.0	0.0	12.697075199479794	0.0
22	2.7094029830526846E-4	0.0	0.0	13.179890811059783	0.0
23	2.7094029830526846E-4	0.0	0.0	13.67191839278215	0.0
24	2.7094029830526846E-4	0.0	0.0	14.077380549195984	0.0
25	2.7094029830526846E-4	0.0	0.0	14.433260631019955	0.0
26	2.7094029830526846E-4	0.0	0.0	14.774916347182899	0.0
27	2.7094029830526846E-4	0.0	0.0	15.104515220071256	0.0
28	2.7094029830526846E-4	0.0	0.0	15.441700421312165	0.0
29	2.7094029830526846E-4	0.0	0.0	15.80042537626834	0.0
30	2.7094029830526846E-4	0.0	0.0	16.196675562539795	0.0
31	2.7094029830526846E-4	0.0	0.0	16.56569624883157	0.0
32	2.7094029830526846E-4	0.0	1.3547014915263423E-4	16.929569069455546	0.0
33	2.7094029830526846E-4	0.0	1.3547014915263423E-4	17.288429494560873	1.3547014915263423E-4
34	2.7094029830526846E-4	0.0	1.3547014915263423E-4	17.643903165937385	1.3547014915263423E-4
35	2.7094029830526846E-4	0.0	1.3547014915263423E-4	18.046384979069863	1.3547014915263423E-4
36	2.7094029830526846E-4	0.0	1.3547014915263423E-4	18.48232791904304	1.3547014915263423E-4
37	2.7094029830526846E-4	0.0	1.3547014915263423E-4	18.851348605334813	1.3547014915263423E-4
38	2.7094029830526846E-4	0.0	1.3547014915263423E-4	19.1977457767181	1.3547014915263423E-4
39	2.7094029830526846E-4	0.0	1.3547014915263423E-4	19.541027134670877	1.3547014915263423E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAATT	20	7.0316443E-4	45.0	15
CGACCGT	20	7.0316443E-4	45.0	24
TCGATTG	20	7.0316443E-4	45.0	1
ATCCGTA	20	7.0316443E-4	45.0	34
ACGATTG	20	7.0316443E-4	45.0	11
CCACTAG	25	3.8894446E-5	45.0	18
ATTTTCG	25	3.8894446E-5	45.0	27
CTACGTT	20	7.0316443E-4	45.0	42
GTATGAT	25	3.8894446E-5	45.0	35
CGTAGAA	25	3.8894446E-5	45.0	42
ACTACGT	20	7.0316443E-4	45.0	41
AGCGATC	25	3.8894446E-5	45.0	45
TACCTAC	45	3.8380676E-10	45.0	24
GAACGGT	20	7.0316443E-4	45.0	21
CATACCG	20	7.0316443E-4	45.0	1
TCCGTAT	25	3.8894446E-5	45.0	15
TTACGCC	20	7.0316443E-4	45.0	13
TTACGAC	20	7.0316443E-4	45.0	13
ATAACGC	20	7.0316443E-4	45.0	11
CGACTCA	25	3.8894446E-5	45.0	25
>>END_MODULE