##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552566_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	921020
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.773723697639575	31.0	31.0	34.0	30.0	34.0
2	31.914319993051183	33.0	31.0	34.0	30.0	34.0
3	31.954588391131573	33.0	31.0	34.0	30.0	34.0
4	35.59181993876354	37.0	35.0	37.0	33.0	37.0
5	35.40860676206814	37.0	35.0	37.0	33.0	37.0
6	35.40259277757269	37.0	35.0	37.0	33.0	37.0
7	35.76417884519337	37.0	35.0	37.0	35.0	37.0
8	35.59062669648868	37.0	35.0	37.0	35.0	37.0
9	37.32885062213633	39.0	37.0	39.0	34.0	39.0
10	36.8644633124145	39.0	37.0	39.0	32.0	39.0
11	36.757446092375844	39.0	37.0	39.0	32.0	39.0
12	36.641679876658486	39.0	35.0	39.0	32.0	39.0
13	36.50805845692819	39.0	35.0	39.0	32.0	39.0
14	37.6372673774728	40.0	36.0	41.0	33.0	41.0
15	37.784179496645024	40.0	36.0	41.0	33.0	41.0
16	37.81815161451434	40.0	36.0	41.0	33.0	41.0
17	37.76898981563918	40.0	36.0	41.0	33.0	41.0
18	37.76949686217455	40.0	36.0	41.0	33.0	41.0
19	37.73213502421228	40.0	36.0	41.0	33.0	41.0
20	37.659778289287964	39.0	36.0	41.0	33.0	41.0
21	37.572257931423856	39.0	36.0	41.0	33.0	41.0
22	37.594689583288094	39.0	36.0	41.0	33.0	41.0
23	37.515108249549414	39.0	35.0	41.0	33.0	41.0
24	37.53217411131137	39.0	35.0	41.0	33.0	41.0
25	37.36956743610345	39.0	35.0	41.0	32.0	41.0
26	37.34456146446331	39.0	35.0	41.0	32.0	41.0
27	37.11796595079368	39.0	35.0	41.0	32.0	41.0
28	37.16134285900415	39.0	35.0	41.0	32.0	41.0
29	37.12830774576013	39.0	35.0	41.0	32.0	41.0
30	36.976397906668694	39.0	35.0	41.0	31.0	41.0
31	36.94590996938177	39.0	35.0	41.0	31.0	41.0
32	36.85438535536687	39.0	35.0	41.0	31.0	41.0
33	36.73288745086969	39.0	35.0	41.0	31.0	41.0
34	36.67415474148227	39.0	35.0	41.0	31.0	41.0
35	36.52705804434214	39.0	35.0	41.0	30.0	41.0
36	36.47205598141191	39.0	35.0	40.0	30.0	41.0
37	36.42897548370285	38.0	35.0	40.0	30.0	41.0
38	36.34962215804217	38.0	35.0	40.0	30.0	41.0
39	36.30190006731667	38.0	35.0	40.0	30.0	41.0
40	36.2471998436516	38.0	35.0	40.0	30.0	41.0
41	36.16138303185598	38.0	35.0	40.0	30.0	41.0
42	36.112024711732644	38.0	35.0	40.0	30.0	41.0
43	36.05533538902521	38.0	35.0	40.0	30.0	41.0
44	35.97375844172765	38.0	35.0	40.0	30.0	41.0
45	35.9361979110117	38.0	35.0	40.0	30.0	41.0
46	35.8275173177564	38.0	35.0	40.0	29.0	41.0
47	35.73821849688389	38.0	34.0	40.0	29.0	41.0
48	35.659400447330135	37.0	34.0	40.0	29.0	41.0
49	35.596901261644696	37.0	34.0	40.0	29.0	41.0
50	35.50361338515993	37.0	34.0	40.0	29.0	41.0
51	34.78380925495646	36.0	33.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	33.0
10	59.0
11	74.0
12	60.0
13	54.0
14	52.0
15	85.0
16	128.0
17	194.0
18	360.0
19	589.0
20	895.0
21	1390.0
22	2095.0
23	3046.0
24	4241.0
25	5605.0
26	7455.0
27	9283.0
28	11593.0
29	14193.0
30	17717.0
31	22454.0
32	29325.0
33	40297.0
34	66579.0
35	82857.0
36	83240.0
37	117262.0
38	177001.0
39	222712.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.68322077696467	19.541920913769516	24.347028294716726	13.427830014549086
2	29.765477405485225	24.905648085817898	26.809841262947604	18.519033245749277
3	32.87702764326508	23.448025015743415	26.612777138390047	17.062170202601465
4	28.876897352934787	27.603743675490218	24.583288093635318	18.936070877939677
5	25.61431890729843	30.374584699572218	24.309244098933792	19.701852294195564
6	24.952986905821806	35.66936657184426	23.37527958133374	16.002366941000197
7	82.28105795748192	6.1162624047251954	7.192134807061736	4.410544830731146
8	84.01174784478079	5.124752991248833	6.076306703437493	4.7871924605328875
9	78.68352478773534	7.005711059477536	8.631734381446657	5.6790297713404705
10	42.41427982019935	26.979978719246056	15.439729864715208	15.166011595839397
11	31.34524766020282	25.720071225380558	22.820785650691626	20.113895463725
12	27.388764630518338	22.273674838765718	29.487307550324637	20.850252980391303
13	24.77546633080715	23.980043864411197	29.68621745456125	21.55827235022041
14	20.372630344617924	28.195370350263836	27.90438861262513	23.527610692493106
15	20.65514321078804	26.15600095546242	31.4928014592517	21.696054374497837
16	23.162797767692343	24.39664719550064	29.924540183709365	22.51601485309765
17	22.193546285639833	24.691211917222212	27.603526524939742	25.511715272198217
18	23.67494734099151	24.707389633232722	28.624676988556164	22.992986037219605
19	25.503789277105817	26.242535449827365	26.139714664176672	22.113960608890142
20	26.271850774141715	25.81550889231504	25.394128249115113	22.51851208442813
21	24.85505200755684	27.11428633471586	25.902043386679985	22.12861827104732
22	23.425441358493842	24.955375561877048	25.25298039130529	26.366202688323813
23	22.03708931402141	27.623612950858828	25.464593602744785	24.874704132374976
24	23.34020976743176	25.608998718811755	27.75194892619053	23.29884258756596
25	23.707628498838247	26.042105491737424	26.058174632472692	24.19209137695164
26	22.877027643265073	27.004082430348962	26.052854443986018	24.066035482399947
27	23.094612494842675	25.4258322294847	27.3784499793707	24.101105296301924
28	22.31178476037437	26.561095307376604	27.977351197585286	23.14976873466374
29	23.471151549369175	24.758962888970924	27.30831035156674	24.46157521009316
30	24.874486981824496	25.43364964930186	26.930794119563096	22.761069249310548
31	25.57175739940501	27.008208290808017	24.58556817441532	22.834466135371652
32	26.87020911598011	26.058608933573645	24.824868081040584	22.24631386940566
33	25.292827517317757	26.536340144622265	24.99229115545808	23.178541182601897
34	24.87698421315498	25.663069205880433	25.779461900935917	23.680484680028663
35	25.037892771058175	24.73768213502421	26.921348070617356	23.303077023300254
36	26.60094243338907	26.965755358189835	25.24168856268051	21.191613645740592
37	24.481335910186534	28.106664350394126	26.494864389481226	20.917135349938114
38	24.667542507220254	27.41851425593364	26.03374519554407	21.880198041302034
39	25.003691559358103	26.134828776790947	26.085318451282273	22.776161212568674
40	25.60465570780222	25.33332609498165	27.65672840980652	21.40528978740961
41	23.817506677379427	26.575535818983305	25.234522594514775	24.372434909122497
42	23.456385311936767	26.195413780373933	26.001715489348765	24.346485418340535
43	23.84855920609759	25.079911402575405	27.102017328613925	23.96951206271308
44	23.403617728170943	25.254717595709103	27.279755054179063	24.06190962194089
45	24.106208334238126	25.261666413324356	26.010835812468784	24.62128943996873
46	23.880480337017655	25.722785607261518	27.132201255130184	23.264532800590647
47	23.958654535189247	25.14950815400317	28.015461119193937	22.876376191613645
48	23.61924822479425	24.438882977568348	27.600594992508302	24.341273805129095
49	23.7066513213611	24.250504875029858	28.728040650583047	23.31480315302599
50	22.70330720288376	24.909665371001715	28.199930511823844	24.187096914290677
51	23.19982193654861	24.224012507871706	26.765108249549414	25.81105730603027
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1334.0
1	1600.5
2	1867.0
3	2544.0
4	3221.0
5	2459.5
6	1698.0
7	1661.5
8	1625.0
9	1644.0
10	1663.0
11	1740.5
12	1818.0
13	1901.0
14	1984.0
15	1978.0
16	1972.0
17	1985.5
18	1999.0
19	2040.5
20	2082.0
21	2320.0
22	2558.0
23	2940.5
24	3323.0
25	4607.0
26	6252.0
27	6613.0
28	8275.0
29	9937.0
30	12918.0
31	15899.0
32	16693.5
33	17488.0
34	19138.5
35	20789.0
36	22766.0
37	24743.0
38	27284.5
39	29826.0
40	32861.5
41	35897.0
42	40125.0
43	44353.0
44	49706.0
45	55059.0
46	60333.5
47	65608.0
48	74069.0
49	82530.0
50	80630.5
51	78731.0
52	72204.5
53	65678.0
54	61911.0
55	58144.0
56	53025.5
57	47907.0
58	46755.0
59	45603.0
60	44454.0
61	43305.0
62	39146.5
63	34988.0
64	31267.5
65	27547.0
66	24002.5
67	20458.0
68	18874.0
69	17290.0
70	15168.0
71	13046.0
72	11213.5
73	9381.0
74	7968.5
75	5506.0
76	4456.0
77	3853.0
78	3250.0
79	2405.0
80	1560.0
81	1151.0
82	742.0
83	612.0
84	482.0
85	275.0
86	68.0
87	48.0
88	28.0
89	18.0
90	8.0
91	10.5
92	13.0
93	7.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	921020.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.23589881334875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.11212785298183	18.204099303001815
2	10.014494670959046	4.854205590243675
3	3.5442823159403316	2.576966027252137
4	1.7163862199501587	1.6639265100535279
5	0.9882911070459072	1.1976061634248512
6	0.6484758178787623	0.9429836583007953
7	0.4564990640222981	0.7744565586822981
8	0.3711539869787045	0.7196200378069473
9	0.3021984087408813	0.6591645050219104
>10	4.774288123328628	31.089942718776925
>50	1.9521885662684697	31.434677408843264
>100	0.11239189664274939	4.015537402234459
>500	0.004513730788865437	0.7402713815054508
>1k	0.0027082384733192622	1.1265427348519481
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	2434	0.26427221993007755	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	2275	0.2470087511671842	Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT	1707	0.1853379948318169	Illumina Single End Adapter 1 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1430	0.1552626435908015	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1346	0.1461423204707824	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1106	0.12008425441358493	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCC	953	0.10347223730212156	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1986927536861306	0.0
2	0.0	0.0	0.0	0.9354845714533886	0.0
3	1.0857527523832273E-4	0.0	0.0	1.2368895355149725	0.0
4	1.0857527523832273E-4	0.0	0.0	1.571409958524245	0.0
5	1.0857527523832273E-4	0.0	0.0	2.430023235108901	0.0
6	1.0857527523832273E-4	0.0	0.0	3.0989555058522074	0.0
7	1.0857527523832273E-4	0.0	0.0	3.7453041193459424	0.0
8	1.0857527523832273E-4	0.0	0.0	4.866886712557816	0.0
9	1.0857527523832273E-4	0.0	0.0	5.341686391175002	0.0
10	1.0857527523832273E-4	0.0	0.0	6.276085209876007	0.0
11	2.1715055047664546E-4	0.0	0.0	7.3395800308353785	0.0
12	2.1715055047664546E-4	0.0	0.0	8.153894595122798	0.0
13	2.1715055047664546E-4	0.0	0.0	8.478860393911098	0.0
14	2.1715055047664546E-4	0.0	0.0	8.610019326398993	0.0
15	2.1715055047664546E-4	0.0	0.0	8.829124231829928	0.0
16	2.1715055047664546E-4	0.0	0.0	9.31771297040238	0.0
17	2.1715055047664546E-4	0.0	0.0	9.91911141994745	0.0
18	2.1715055047664546E-4	0.0	0.0	10.599769820416494	0.0
19	2.1715055047664546E-4	0.0	0.0	10.973377342511563	0.0
20	2.1715055047664546E-4	0.0	0.0	11.34068749864281	0.0
21	2.1715055047664546E-4	0.0	0.0	11.776400078174198	0.0
22	2.1715055047664546E-4	0.0	0.0	12.219495776421793	0.0
23	2.1715055047664546E-4	0.0	0.0	12.676597685175132	0.0
24	2.1715055047664546E-4	0.0	0.0	13.065840046904519	0.0
25	2.1715055047664546E-4	0.0	0.0	13.383422726976614	0.0
26	2.1715055047664546E-4	0.0	0.0	13.671581507459122	0.0
27	2.1715055047664546E-4	0.0	0.0	13.985146902347397	0.0
28	3.257258257149682E-4	0.0	0.0	14.309678400034745	0.0
29	3.257258257149682E-4	0.0	0.0	14.650604764283077	0.0
30	3.257258257149682E-4	0.0	0.0	15.013246183579076	0.0
31	3.257258257149682E-4	0.0	0.0	15.341360665349287	0.0
32	3.257258257149682E-4	0.0	0.0	15.669692297669975	0.0
33	4.343011009532909E-4	0.0	0.0	16.01322446852403	0.0
34	4.343011009532909E-4	0.0	0.0	16.341013224468526	0.0
35	4.343011009532909E-4	0.0	0.0	16.71396929491216	0.0
36	4.343011009532909E-4	0.0	0.0	17.044689583288093	0.0
37	5.428763761916136E-4	0.0	0.0	17.37247833923259	0.0
38	5.428763761916136E-4	0.0	0.0	17.71481618205902	0.0
39	5.428763761916136E-4	0.0	0.0	18.04521074460924	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGATT	20	7.0325553E-4	45.000004	18
CATAGGT	20	7.0325553E-4	45.000004	13
TTACGAA	20	7.0325553E-4	45.000004	21
AGTCGAC	20	7.0325553E-4	45.000004	16
TCACGTA	20	7.0325553E-4	45.000004	14
ACGAACA	20	7.0325553E-4	45.000004	26
TAGGTCG	25	3.8902006E-5	45.0	1
ACGGCAC	25	3.8902006E-5	45.0	18
CTCGACG	55	1.8189894E-12	45.0	1
TTAACGG	110	0.0	42.954544	2
TACGAAT	250	0.0	42.3	12
ATAGCGG	115	0.0	41.086956	2
GTTAACG	50	1.0804797E-9	40.5	1
CGTTTAT	50	1.0804797E-9	40.5	39
GCGTAAG	95	0.0	40.26316	1
GCTACGA	270	0.0	40.0	10
CGAATAT	265	0.0	39.905663	14
ACGCATT	85	0.0	39.705883	17
CGTAAGG	125	0.0	39.6	2
CCCTCGT	120	0.0	39.375004	14
>>END_MODULE