FastQCFastQC Report
Sat 18 Jun 2016
SRR3552565_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552565_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences675183
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC16660.24674791871240834TruSeq Adapter, Index 14 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12590.1864679649813458No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC12470.18469066904824322TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11920.17654472935485638No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT8300.12292963537292852Illumina PCR Primer Index 8 (95% over 21bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC7760.11493180367396691No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC6920.10249073214224885No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTTC302.1641645E-645.00000442
TATTACG302.1641645E-645.0000041
GTTGGTA302.1641645E-645.00000443
AATTTCG207.031217E-445.032
GAATGCG207.031217E-445.01
GCGAATG207.031217E-445.01
CGACGAA207.031217E-445.037
ATAGCGC207.031217E-445.012
TAACGAG207.031217E-445.01
ATCACGT253.8890903E-545.016
TCGGCTA253.8890903E-545.043
TAGCACG453.8380676E-1045.01
AATGACG207.031217E-445.01
CTACGAT253.8890903E-545.031
GAACGCT253.8890903E-545.021
ACTAACG207.031217E-445.01
ACGTCGT207.031217E-445.01
AATTACG207.031217E-445.023
CGTAACA253.8890903E-545.018
TTCGGAT207.031217E-445.031