##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552565_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	675183
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76163203161217	31.0	31.0	34.0	30.0	34.0
2	31.911535094929818	33.0	31.0	34.0	30.0	34.0
3	31.950349757028835	33.0	31.0	34.0	30.0	34.0
4	35.58389947614202	37.0	35.0	37.0	33.0	37.0
5	35.39464708086548	37.0	35.0	37.0	33.0	37.0
6	35.40327437154075	37.0	35.0	37.0	33.0	37.0
7	35.77453371900655	37.0	35.0	37.0	35.0	37.0
8	35.60752418233279	37.0	35.0	37.0	35.0	37.0
9	37.327899251017875	39.0	37.0	39.0	34.0	39.0
10	36.86042154497373	39.0	37.0	39.0	32.0	39.0
11	36.75179173646256	39.0	37.0	39.0	32.0	39.0
12	36.57108961570419	39.0	35.0	39.0	32.0	39.0
13	36.371194179948255	39.0	35.0	39.0	32.0	39.0
14	37.48199228949781	39.0	36.0	41.0	32.0	41.0
15	37.64582639077109	39.0	36.0	41.0	33.0	41.0
16	37.69605869816035	40.0	36.0	41.0	33.0	41.0
17	37.66608015900874	39.0	36.0	41.0	33.0	41.0
18	37.64662617394099	39.0	36.0	41.0	33.0	41.0
19	37.59247196685936	39.0	36.0	41.0	33.0	41.0
20	37.524397089381694	39.0	35.0	41.0	33.0	41.0
21	37.44050131593953	39.0	35.0	41.0	32.0	41.0
22	37.43869143624765	39.0	35.0	41.0	33.0	41.0
23	37.361318338879975	39.0	35.0	41.0	32.0	41.0
24	37.33970642033345	39.0	35.0	41.0	32.0	41.0
25	37.15515497279997	39.0	35.0	41.0	32.0	41.0
26	37.139465892950504	39.0	35.0	41.0	32.0	41.0
27	36.8981535376335	39.0	35.0	41.0	31.0	41.0
28	36.92887409783718	39.0	35.0	41.0	31.0	41.0
29	36.9227735295468	39.0	35.0	41.0	31.0	41.0
30	36.767154978724285	39.0	35.0	41.0	31.0	41.0
31	36.74064216664223	39.0	35.0	41.0	31.0	41.0
32	36.58837381865361	39.0	35.0	40.0	31.0	41.0
33	36.436475740651055	39.0	35.0	40.0	30.0	41.0
34	36.374369615348726	38.0	35.0	40.0	30.0	41.0
35	36.189964794729725	38.0	35.0	40.0	30.0	41.0
36	36.105710599941055	38.0	35.0	40.0	30.0	41.0
37	36.03944263999538	38.0	35.0	40.0	30.0	41.0
38	35.94869835289099	38.0	35.0	40.0	30.0	41.0
39	35.93706595100884	38.0	35.0	40.0	29.0	41.0
40	35.7967958316486	38.0	35.0	40.0	29.0	41.0
41	35.66187981628684	38.0	34.0	40.0	29.0	41.0
42	35.592535653296956	38.0	34.0	40.0	29.0	41.0
43	35.526143282635964	38.0	34.0	40.0	28.0	41.0
44	35.462473136912514	37.0	34.0	40.0	28.0	41.0
45	35.35341085305762	37.0	34.0	40.0	28.0	41.0
46	35.195628444436544	37.0	34.0	40.0	28.0	41.0
47	35.05054629633744	37.0	34.0	40.0	28.0	41.0
48	34.93925794932633	36.0	34.0	40.0	27.0	41.0
49	34.84613208567159	36.0	34.0	40.0	27.0	41.0
50	34.7382546657721	36.0	34.0	40.0	27.0	41.0
51	33.97824885993872	35.0	33.0	39.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	39.0
10	38.0
11	26.0
12	33.0
13	40.0
14	51.0
15	64.0
16	108.0
17	164.0
18	292.0
19	513.0
20	840.0
21	1291.0
22	1857.0
23	2800.0
24	3767.0
25	5079.0
26	6348.0
27	7757.0
28	9467.0
29	11278.0
30	13886.0
31	17860.0
32	22800.0
33	31345.0
34	52264.0
35	68340.0
36	62927.0
37	86125.0
38	123388.0
39	144307.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.44212309847848	20.03382786592672	24.352656983365993	13.171392052228803
2	31.893575519525818	24.601626521994778	24.864221996110683	18.64057596236872
3	32.07115700484165	23.91248002393425	26.180309634573145	17.836053336650952
4	29.472306026662405	26.460529960025653	24.07258476590791	19.994579247404037
5	25.38200754462124	29.779926331083573	24.647095676283318	20.19097044801187
6	24.386425606094942	35.416176058935136	24.051997754682805	16.145400580287124
7	81.05446967710976	6.630054370444753	7.68813788261849	4.627338069826995
8	82.59316363119332	5.1214263392295125	7.1755361139128215	5.109873915664346
9	77.55808425271371	7.315498168644649	9.237939936283942	5.888477642357701
10	42.80943092465302	24.631840552857522	15.934791012214466	16.623937510274992
11	32.96513685919225	26.19289881409929	21.10198272172137	19.739981604987094
12	28.70599526350634	23.211632994314137	28.132224893103054	19.95014684907647
13	24.582816806702777	26.475340759468175	28.907866459907904	20.033975973921144
14	21.52942239363254	28.796637356094568	28.137408672907938	21.53653157736495
15	20.02109057840615	26.18223503850067	32.68462031775089	21.112054065342285
16	22.166286769660967	23.895891928558626	32.017097586876446	21.920723714903957
17	21.936571270307457	24.30289269723912	27.290379052790133	26.47015697966329
18	24.166040910390223	23.962688634044397	28.37438146398828	23.496888991577098
19	24.25949705487253	27.20106993215173	25.74057699912468	22.79885601385106
20	26.08226214226365	25.57410361339074	25.31121192328598	23.032422321059624
21	24.972933264018792	26.73008650987954	26.223557168945312	22.073423057156354
22	23.23592270539987	25.46183775361643	25.019883498251584	26.28235604273212
23	22.222864023531397	27.677237134228793	24.897546294856358	25.202352547383448
24	22.993173702536943	25.24604440573889	28.372456060060752	23.388325831663415
25	21.914355071143675	26.821913466423176	26.911963127033705	24.35176833539944
26	22.643046403715733	27.30474552824938	26.048197303545855	24.004010764489035
27	22.768641982988317	27.262534749838192	25.864395282464166	24.104427984709332
28	21.188181574476843	27.923244512969074	27.894955886033863	22.993618026520217
29	23.58886405611516	25.520932843392085	26.581386083476627	24.308817017016125
30	23.835315758838714	26.967503624943163	25.512342579715426	23.684838036502693
31	26.67395357999239	27.917764517175343	22.617868044663446	22.790413858168822
32	27.10568838374189	27.106132707725166	23.56250083310747	22.225678075425478
33	25.782343453552592	26.726087594030062	22.484867065669604	25.00670188674774
34	24.489656878209313	26.656328728655787	24.781725843215842	24.07228854991906
35	25.016180798390952	25.552627954199085	25.350312433814242	24.08087881359572
36	26.85568208915213	27.726260880383542	23.555835973358334	21.862221057105998
37	25.9603692628517	27.757511667207265	25.442435606346724	20.839683463594312
38	24.81075501012318	27.30622660819363	24.927019785746975	22.955998595936215
39	25.43162372275368	25.794932633078737	24.44892717974238	24.3245164644252
40	26.35907598384438	23.92551352744367	27.20566127997891	22.50974920873304
41	22.812481949338178	26.151872899643504	26.364852195626966	24.670792955391352
42	24.473661214811393	25.84410448722791	26.003320581235013	23.67891371672569
43	24.09820744894347	25.61823979572945	26.792884299515833	23.49066845581124
44	24.86570307605494	24.760842616001884	25.470576125287515	24.902878182655666
45	25.455913433839417	24.93872031730657	24.373095886596673	25.232270362257342
46	25.536928506790012	25.577954421245796	24.892658731040328	23.992458340923868
47	23.821689823351594	26.109514013237895	26.98098145243586	23.087814710974655
48	23.60915485135141	24.533793060548028	27.281936897107894	24.575115190992662
49	22.99228505457039	24.321258088547847	28.435253849697045	24.251203007184717
50	23.240365945232625	24.877107391625678	27.189073184603284	24.69345347853841
51	22.748203079757637	24.490545526175865	25.581953337095275	27.17929805697122
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1639.0
1	1662.5
2	1686.0
3	2225.0
4	2764.0
5	2105.5
6	1447.0
7	1462.0
8	1477.0
9	1591.5
10	1706.0
11	1821.5
12	1937.0
13	1877.5
14	1818.0
15	1977.0
16	2136.0
17	2158.0
18	2180.0
19	2246.0
20	2312.0
21	2229.0
22	2146.0
23	2092.5
24	2039.0
25	2633.0
26	3909.5
27	4592.0
28	5447.5
29	6303.0
30	7497.5
31	8692.0
32	9221.5
33	9751.0
34	12249.5
35	14748.0
36	14951.5
37	15155.0
38	16691.5
39	18228.0
40	21105.5
41	23983.0
42	27876.5
43	31770.0
44	36499.0
45	41228.0
46	42539.5
47	43851.0
48	46423.5
49	48996.0
50	54068.0
51	59140.0
52	59407.5
53	59675.0
54	55245.0
55	50815.0
56	45587.5
57	40360.0
58	37724.5
59	35089.0
60	32809.0
61	30529.0
62	28703.5
63	26878.0
64	23208.5
65	19539.0
66	17254.0
67	14969.0
68	13695.0
69	12421.0
70	10762.5
71	9104.0
72	8012.0
73	6920.0
74	6328.0
75	4784.0
76	3832.0
77	2968.0
78	2104.0
79	1627.0
80	1150.0
81	809.5
82	469.0
83	370.0
84	271.0
85	285.5
86	300.0
87	171.0
88	42.0
89	30.5
90	19.0
91	12.5
92	6.0
93	4.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	675183.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.635076808650126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.36658516158992	19.32028199423029
2	10.294269589789268	5.277887832463982
3	3.799395355016235	2.9219337525680906
4	1.822366813573604	1.868660529579773
5	1.0125326967658108	1.2978176726430604
6	0.6667955447219094	1.0256013002767108
7	0.4745806923876738	0.8516138750882264
8	0.3814535276113233	0.7822872383397451
9	0.2874688332185929	0.6632357057686495
>10	3.876078193419602	26.056637679657097
>50	1.853024936831782	32.64438279021155
>100	0.1590629175099963	5.8040179486642485
>500	0.004063651177262679	0.6873864491905377
>1k	0.0023220863870072454	0.7982552313180439
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	1666	0.24674791871240834	TruSeq Adapter, Index 14 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1259	0.1864679649813458	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	1247	0.18469066904824322	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1192	0.17654472935485638	No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	830	0.12292963537292852	Illumina PCR Primer Index 8 (95% over 21bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	776	0.11493180367396691	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC	692	0.10249073214224885	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13122368306074056	0.0
2	0.0	0.0	0.0	0.751944287696817	0.0
3	0.0	0.0	0.0	1.04653108860857	0.0
4	0.0	0.0	0.0	1.385402179853462	0.0
5	0.0	0.0	0.0	2.387352762140042	0.0
6	0.0	0.0	0.0	3.1335208380542756	0.0
7	0.0	0.0	0.0	3.8330348957245666	0.0
8	0.0	0.0	0.0	4.934514050264891	0.0
9	0.0	0.0	0.0	5.42638069975103	0.0
10	0.0	0.0	0.0	6.491425287662752	0.0
11	0.0	0.0	0.0	7.92185229782148	0.0
12	0.0	0.0	0.0	9.0379941438099	0.0
13	0.0	0.0	0.0	9.458768955971937	0.0
14	0.0	0.0	0.0	9.624057477750476	0.0
15	0.0	0.0	0.0	9.916570766740277	0.0
16	0.0	0.0	0.0	10.537587587365204	0.0
17	0.0	0.0	0.0	11.293382682917075	0.0
18	0.0	0.0	0.0	12.13048906740839	0.0
19	0.0	0.0	0.0	12.57970061450007	0.0
20	0.0	0.0	0.0	12.981073279392401	0.0
21	0.0	0.0	0.0	13.487750728321062	0.0
22	0.0	0.0	0.0	13.999019525076905	0.0
23	0.0	0.0	0.0	14.530727225063428	0.0
24	0.0	0.0	0.0	14.950465281264487	0.0
25	0.0	0.0	0.0	15.29422393632541	0.0
26	0.0	0.0	0.0	15.608805316484569	0.0
27	0.0	0.0	0.0	15.959080723300202	0.0
28	0.0	0.0	0.0	16.29276803474021	0.0
29	0.0	0.0	0.0	16.62556669821367	0.0
30	0.0	0.0	0.0	17.021459367312268	0.0
31	0.0	0.0	0.0	17.378103417888187	0.0
32	0.0	0.0	0.0	17.724231800859915	0.0
33	0.0	0.0	0.0	18.08132017541911	0.0
34	0.0	0.0	0.0	18.422264778585955	0.0
35	0.0	0.0	0.0	18.800828812336803	0.0
36	0.0	0.0	0.0	19.140292335559398	0.0
37	0.0	0.0	0.0	19.487901798475377	0.0
38	0.0	0.0	0.0	19.8158128981328	0.0
39	0.0	0.0	0.0	20.15142561350034	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTTC	30	2.1641645E-6	45.000004	42
TATTACG	30	2.1641645E-6	45.000004	1
GTTGGTA	30	2.1641645E-6	45.000004	43
AATTTCG	20	7.031217E-4	45.0	32
GAATGCG	20	7.031217E-4	45.0	1
GCGAATG	20	7.031217E-4	45.0	1
CGACGAA	20	7.031217E-4	45.0	37
ATAGCGC	20	7.031217E-4	45.0	12
TAACGAG	20	7.031217E-4	45.0	1
ATCACGT	25	3.8890903E-5	45.0	16
TCGGCTA	25	3.8890903E-5	45.0	43
TAGCACG	45	3.8380676E-10	45.0	1
AATGACG	20	7.031217E-4	45.0	1
CTACGAT	25	3.8890903E-5	45.0	31
GAACGCT	25	3.8890903E-5	45.0	21
ACTAACG	20	7.031217E-4	45.0	1
ACGTCGT	20	7.031217E-4	45.0	1
AATTACG	20	7.031217E-4	45.0	23
CGTAACA	25	3.8890903E-5	45.0	18
TTCGGAT	20	7.031217E-4	45.0	31
>>END_MODULE