FastQCFastQC Report
Sat 18 Jun 2016
SRR3552562_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552562_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722317
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCG32470.44952562379121636No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGC31120.4308357687829582No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC30780.42612869418828575No Hit
GAATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTC27220.37684285431465686No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC23660.32755701444102797No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19350.26788792178503346No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT19070.26401150741295026Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13360.18496034289653987No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC11090.1535336978085799No Hit
TCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC10350.1432888883966458No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCT8120.11241601679041195No Hit
ACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC7840.1085396024183288No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7250.10037144356286783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGA700.045.00000441
CAGGTCG207.031545E-445.034
ACGCAGT253.889361E-545.011
CGCATCG207.031545E-445.021
GTCTCGA207.031545E-445.027
GAATACG453.8380676E-1045.01
ATCGTAG207.031545E-445.02
ACGATAT207.031545E-445.029
TCGTGCG253.889361E-545.01
CGCTATC207.031545E-445.026
TACGAGC207.031545E-445.040
CTACGAT207.031545E-445.027
CGCTAGG253.889361E-545.02
CCGAGAC207.031545E-445.021
TTGCGGT253.889361E-545.012
GTCCGAC207.031545E-445.041
TCGTAGC207.031545E-445.026
TCCCGCT253.889361E-545.041
CGGCTAG207.031545E-445.014
GCGTGAT207.031545E-445.033