##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552562_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	722317
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61304385747532	31.0	31.0	34.0	30.0	34.0
2	31.76159913168318	31.0	31.0	34.0	30.0	34.0
3	31.80983557080894	33.0	31.0	34.0	30.0	34.0
4	35.47094419763068	37.0	35.0	37.0	33.0	37.0
5	35.30054948173724	37.0	35.0	37.0	33.0	37.0
6	35.30453111307085	37.0	35.0	37.0	32.0	37.0
7	35.744619052299754	37.0	35.0	37.0	35.0	37.0
8	35.54704098062208	37.0	35.0	37.0	35.0	37.0
9	37.28421870176114	39.0	37.0	39.0	34.0	39.0
10	36.72534911956939	39.0	37.0	39.0	32.0	39.0
11	36.6295587671341	39.0	35.0	39.0	32.0	39.0
12	36.64388350267265	39.0	35.0	39.0	32.0	39.0
13	36.508460966583925	39.0	35.0	39.0	32.0	39.0
14	37.61093398050994	39.0	36.0	41.0	32.0	41.0
15	37.73645920004652	40.0	36.0	41.0	33.0	41.0
16	37.79996732736458	40.0	36.0	41.0	33.0	41.0
17	37.73377893639496	39.0	36.0	41.0	33.0	41.0
18	37.69711082530247	39.0	36.0	41.0	33.0	41.0
19	37.67975833325257	39.0	36.0	41.0	33.0	41.0
20	37.55030270642945	39.0	36.0	41.0	32.0	41.0
21	37.508765541998876	39.0	36.0	41.0	32.0	41.0
22	37.5524693451767	39.0	36.0	41.0	32.0	41.0
23	37.41712433737542	39.0	36.0	41.0	32.0	41.0
24	37.41494800759224	39.0	35.0	41.0	32.0	41.0
25	37.31316582608467	39.0	35.0	41.0	32.0	41.0
26	37.29004024548779	39.0	35.0	41.0	32.0	41.0
27	37.03964741242419	39.0	35.0	41.0	31.0	41.0
28	37.10329536754638	39.0	35.0	41.0	31.0	41.0
29	37.100271764336156	39.0	35.0	41.0	31.0	41.0
30	36.92436838673325	39.0	35.0	41.0	31.0	41.0
31	36.88091793492331	39.0	35.0	41.0	31.0	41.0
32	36.745464941293086	39.0	35.0	41.0	31.0	41.0
33	36.58366478983604	39.0	35.0	40.0	30.0	41.0
34	36.47680450550105	39.0	35.0	40.0	30.0	41.0
35	36.3285759576474	39.0	35.0	40.0	30.0	41.0
36	36.20953127228073	39.0	35.0	40.0	30.0	41.0
37	36.13899160617845	39.0	35.0	40.0	30.0	41.0
38	36.17326603139619	39.0	35.0	40.0	30.0	41.0
39	36.067032895529245	38.0	35.0	40.0	30.0	41.0
40	35.9820992722032	38.0	35.0	40.0	29.0	41.0
41	35.87048622696129	38.0	35.0	40.0	29.0	41.0
42	35.92369832082036	38.0	35.0	40.0	29.0	41.0
43	35.902761529909995	38.0	35.0	40.0	29.0	41.0
44	35.92434900466139	38.0	35.0	40.0	29.0	41.0
45	35.89917723104953	38.0	35.0	40.0	29.0	41.0
46	35.76892278597901	38.0	35.0	40.0	28.0	41.0
47	35.64056086178229	38.0	34.0	40.0	28.0	41.0
48	35.624325607731784	38.0	34.0	40.0	28.0	41.0
49	35.61671537565916	38.0	34.0	40.0	28.0	41.0
50	35.5338293297818	38.0	34.0	40.0	28.0	41.0
51	34.87960964507273	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	17.0
10	39.0
11	45.0
12	28.0
13	22.0
14	34.0
15	54.0
16	88.0
17	134.0
18	262.0
19	442.0
20	868.0
21	1346.0
22	2160.0
23	3115.0
24	4368.0
25	5619.0
26	7051.0
27	8152.0
28	9443.0
29	11336.0
30	14272.0
31	18245.0
32	23771.0
33	31463.0
34	47811.0
35	61687.0
36	64999.0
37	91649.0
38	142734.0
39	170994.0
40	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.544940794692636	20.79364046533586	23.98337572007858	14.678043019892929
2	28.07230066577417	27.847330188822912	26.513012984603712	17.567356160799203
3	29.875110235533707	27.49485336770421	27.60560806404944	15.024428332712644
4	26.505813929341272	27.795690811651948	28.143875888287273	17.554619370719504
5	25.44796813587386	29.80754987076311	25.29595731514003	19.448524678223
6	23.23868883052732	37.0031440489425	23.918999552827913	15.839167567702269
7	79.6459172357843	5.55905509630813	10.4814091320016	4.31361853590598
8	80.24759212367977	6.427510359025193	7.977107004265441	5.347790513029598
9	75.13529378375422	7.278798643808742	10.138484903442672	7.447422668994361
10	37.18990415565465	32.06320770520423	16.947960521488486	13.798927617652637
11	25.702565494097467	26.485462753887834	28.521272516083656	19.29069923593104
12	23.228444021115383	23.067711268044363	32.18517631455441	21.518668396285843
13	22.64075191363349	25.442153514315734	33.26406550032742	18.653029071723356
14	18.755338722472267	29.863619435787886	29.97132837798363	21.40971346375622
15	17.441095806965638	27.450413045795685	34.78708101844481	20.321410128793868
16	19.803770366750335	25.88088055521329	32.090065719067944	22.225283358968433
17	19.537959095521774	26.479786575700142	29.9453010243425	24.03695330443559
18	20.31808748790351	25.398405409259368	31.31118331702009	22.972323785817032
19	20.469267648414753	28.031598314867296	28.860181886900072	22.63895214981788
20	22.5600394286719	27.09336759345274	30.182177631150864	20.1644153467245
21	21.555494332820633	29.502559125702426	29.19646083367829	19.745485707798654
22	19.80072461260084	25.31520094363001	29.450504418420167	25.433570025348985
23	19.059498807310362	28.94975474756928	29.099273587635345	22.89147285748501
24	20.028740843701588	27.527110673014754	30.00386257003504	22.440285913248616
25	18.605266108924475	30.19311465741496	27.605054290567715	23.59656494309285
26	18.58491493347104	28.77919251519762	28.589109767595115	24.046782783736226
27	20.539458437223544	28.683251259488564	28.452050830867886	22.32523947242
28	18.082365498804542	28.33811193700273	30.142444383837013	23.437078180355716
29	20.968217555450032	25.12373376232319	28.643518012174713	25.26453067005207
30	21.346444843469005	28.186793333121052	28.037828266536714	22.42893355687323
31	21.41012879386751	27.246070631038727	26.18739417734873	25.156406397745034
32	23.684753370057745	28.09085207741199	25.03997552321211	23.18441902931815
33	22.865860833955175	27.106935043755026	25.94207252494403	24.085131597345764
34	21.244412079460957	25.08704626915883	28.370230798942845	25.298310852437368
35	22.8409410272775	25.933350592606846	26.53890189487441	24.686806485241245
36	22.52002929461718	26.95257068572386	27.372469428242724	23.154930591416235
37	21.97733128252554	26.81440420203318	29.369376603347284	21.838887912094
38	23.3817008321831	25.975852707329327	27.566013260106022	23.076433200381548
39	24.079732305898936	24.404105122820035	29.291432985794323	22.224729585486706
40	24.652749416115086	23.864175978137023	29.583548497404877	21.89952610834301
41	20.269216978141177	25.497946192599645	29.239932051993794	24.992904777265384
42	22.3664955968086	25.158898378412804	29.22719526191409	23.247410762864504
43	22.223068265041526	25.511790529642802	28.833462316406784	23.431678888908884
44	21.546910843853876	25.684983186052662	28.149828953215835	24.61827701687763
45	22.55311726015032	24.68999068276117	27.086306981560725	25.67058507552778
46	24.087762021383963	25.705611248246964	27.51035902519254	22.69626770517654
47	20.711405103299523	26.0731783967427	30.848367129667448	22.367049370290328
48	21.63828346833869	24.68832936231599	28.821833073290538	24.85155409605478
49	22.014572549171625	23.48124161552338	30.485091725655078	24.01909410964992
50	21.375241064518764	23.37284045647548	30.034181668159547	25.21773681084621
51	19.987346275942556	23.63228333266419	27.846914858711617	28.533455532681636
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1642.0
1	1639.5
2	1637.0
3	2760.0
4	3883.0
5	2837.0
6	1791.0
7	1745.0
8	1699.0
9	1776.0
10	1853.0
11	1950.0
12	2047.0
13	2064.5
14	2082.0
15	2246.5
16	2411.0
17	2214.5
18	2018.0
19	2437.5
20	2857.0
21	2815.0
22	2773.0
23	3244.0
24	3715.0
25	4515.5
26	6417.5
27	7519.0
28	8703.5
29	9888.0
30	10927.0
31	11966.0
32	13664.5
33	15363.0
34	17453.5
35	19544.0
36	21795.5
37	24047.0
38	25646.0
39	27245.0
40	29318.0
41	31391.0
42	35341.5
43	39292.0
44	42304.5
45	45317.0
46	50095.0
47	54873.0
48	65359.5
49	75846.0
50	76683.0
51	77520.0
52	74560.0
53	71600.0
54	60974.5
55	50349.0
56	43992.5
57	37636.0
58	31311.0
59	24986.0
60	22685.0
61	20384.0
62	17499.0
63	14614.0
64	12037.5
65	9461.0
66	7985.0
67	6509.0
68	4885.5
69	3262.0
70	2986.5
71	2711.0
72	2366.0
73	2021.0
74	1686.0
75	1126.0
76	901.0
77	594.0
78	287.0
79	308.0
80	329.0
81	295.5
82	262.0
83	177.0
84	92.0
85	52.0
86	12.0
87	8.5
88	5.0
89	5.0
90	5.0
91	3.0
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	722317.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.168626292023376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.48382382819248	22.47074645873645
2	10.479933249556254	6.3233037954242555
3	3.975829965098888	3.5983598525309004
4	1.94305554600521	2.344772665282985
5	1.1309876339685982	1.7060171635049182
6	0.7508944380295224	1.359207221140294
7	0.5399856493595651	1.1403437681009003
8	0.43393208566598745	1.047290794286036
9	0.36861040351193747	1.0008422559813224
>10	5.034131166794017	36.8706714545783
>50	0.7848996457553267	15.022237214122894
>100	0.0660627718306046	3.4193586102298945
>500	0.0032338419777219038	0.6519833528432155
>1k	0.004619774253888434	3.04486539323765
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCG	3247	0.44952562379121636	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGC	3112	0.4308357687829582	No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	3078	0.42612869418828575	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTC	2722	0.37684285431465686	No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	2366	0.32755701444102797	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1935	0.26788792178503346	No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	1907	0.26401150741295026	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1336	0.18496034289653987	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC	1109	0.1535336978085799	No Hit
TCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	1035	0.1432888883966458	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCT	812	0.11241601679041195	No Hit
ACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	784	0.1085396024183288	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	725	0.10037144356286783	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3844337043154183E-4	0.0	0.0	0.2822860323099138	0.0
2	1.3844337043154183E-4	0.0	0.0	1.368789603456654	0.0
3	1.3844337043154183E-4	0.0	0.0	1.7542159467380665	0.0
4	1.3844337043154183E-4	0.0	0.0	2.303697683980856	0.0
5	1.3844337043154183E-4	0.0	0.0	4.009873781179177	0.0
6	1.3844337043154183E-4	0.0	0.0	5.043215098080206	0.0
7	1.3844337043154183E-4	0.0	0.0	6.148685410976067	0.0
8	1.3844337043154183E-4	0.0	0.0	7.713926157075079	0.0
9	1.3844337043154183E-4	0.0	0.0	8.370978393143178	0.0
10	1.3844337043154183E-4	0.0	0.0	10.126578773585559	0.0
11	1.3844337043154183E-4	0.0	0.0	11.832893314154312	0.0
12	1.3844337043154183E-4	0.0	0.0	13.679450988970217	0.0
13	1.3844337043154183E-4	0.0	0.0	14.243192393367455	0.0
14	1.3844337043154183E-4	0.0	0.0	14.466639993243964	0.0
15	1.3844337043154183E-4	0.0	0.0	14.86771043738414	0.0
16	1.3844337043154183E-4	0.0	0.0	15.580832238477011	0.0
17	1.3844337043154183E-4	0.0	0.0	16.494835370066053	0.0
18	1.3844337043154183E-4	0.0	0.0	17.524161829224564	0.0
19	2.7688674086308367E-4	0.0	0.0	18.258465465993464	0.0
20	2.7688674086308367E-4	0.0	0.0	18.83812785799033	0.0
21	2.7688674086308367E-4	0.0	0.0	19.45143198900206	0.0
22	2.7688674086308367E-4	0.0	0.0	20.17258350557996	0.0
23	2.7688674086308367E-4	0.0	0.0	20.874076063556583	0.0
24	2.7688674086308367E-4	0.0	0.0	21.399607097714714	0.0
25	2.7688674086308367E-4	0.0	0.0	21.867545689773326	0.0
26	2.7688674086308367E-4	0.0	0.0	22.306134287300452	0.0
27	2.7688674086308367E-4	0.0	0.0	22.757182788166414	0.0
28	2.7688674086308367E-4	0.0	0.0	23.232181992117034	0.0
29	2.7688674086308367E-4	0.0	0.0	23.70593520573377	0.0
30	2.7688674086308367E-4	0.0	0.0	24.279782976172513	0.0
31	2.7688674086308367E-4	0.0	0.0	24.757689490902195	0.0
32	4.153301112946255E-4	0.0	0.0	25.216767707253187	0.0
33	4.153301112946255E-4	0.0	0.0	25.65341809759427	0.0
34	5.537734817261673E-4	0.0	0.0	26.078577688189533	0.0
35	5.537734817261673E-4	0.0	0.0	26.549285147656775	0.0
36	5.537734817261673E-4	0.0	0.0	27.011547561527696	0.0
37	5.537734817261673E-4	0.0	0.0	27.473256201916886	0.0
38	5.537734817261673E-4	0.0	0.0	27.91447522348221	0.0
39	5.537734817261673E-4	0.0	0.0	28.323298496366554	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCGA	70	0.0	45.000004	41
CAGGTCG	20	7.031545E-4	45.0	34
ACGCAGT	25	3.889361E-5	45.0	11
CGCATCG	20	7.031545E-4	45.0	21
GTCTCGA	20	7.031545E-4	45.0	27
GAATACG	45	3.8380676E-10	45.0	1
ATCGTAG	20	7.031545E-4	45.0	2
ACGATAT	20	7.031545E-4	45.0	29
TCGTGCG	25	3.889361E-5	45.0	1
CGCTATC	20	7.031545E-4	45.0	26
TACGAGC	20	7.031545E-4	45.0	40
CTACGAT	20	7.031545E-4	45.0	27
CGCTAGG	25	3.889361E-5	45.0	2
CCGAGAC	20	7.031545E-4	45.0	21
TTGCGGT	25	3.889361E-5	45.0	12
GTCCGAC	20	7.031545E-4	45.0	41
TCGTAGC	20	7.031545E-4	45.0	26
TCCCGCT	25	3.889361E-5	45.0	41
CGGCTAG	20	7.031545E-4	45.0	14
GCGTGAT	20	7.031545E-4	45.0	33
>>END_MODULE