FastQCFastQC Report
Sat 18 Jun 2016
SRR3552559_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552559_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723724
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC19690.27206504136936177No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC17180.23738331187027095No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT13760.19012772824999585No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12330.17036881463099193No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10980.15171529478088333No Hit
TCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC8090.11178294487953971No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCC7720.10667049869839884No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7480.10335431739171286No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGT302.1643827E-645.00000428
ACTATCC207.0315535E-445.034
TCCGATT207.0315535E-445.015
TAGCGAA207.0315535E-445.01
TCGATAG253.8893682E-545.01
GGTATGC1050.045.08
GTCGTAA207.0315535E-445.031
TCGTAAG253.8893682E-545.01
CGGTCTA253.8893682E-545.031
ATAACCG207.0315535E-445.015
ACGTAGA207.0315535E-445.044
ATACGCG207.0315535E-445.01
TGCGTAT207.0315535E-445.010
ACGGTCT207.0315535E-445.030
TACGAAT1450.041.89655312
TTGCGAG700.041.7857131
GCTACGA1500.040.510
CAACGTC451.9266736E-840.020
GTATGCG451.9266736E-840.01
AGTTACG451.9266736E-840.01