##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552559_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	723724
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73554006776064	31.0	31.0	34.0	30.0	34.0
2	31.886691611719385	33.0	31.0	34.0	30.0	34.0
3	31.91151322879993	33.0	31.0	34.0	30.0	34.0
4	35.54569559666392	37.0	35.0	37.0	33.0	37.0
5	35.36562695171087	37.0	35.0	37.0	33.0	37.0
6	35.371532517921196	37.0	35.0	37.0	33.0	37.0
7	35.74350442986553	37.0	35.0	37.0	35.0	37.0
8	35.588287800321666	37.0	35.0	37.0	35.0	37.0
9	37.30464514096534	39.0	37.0	39.0	34.0	39.0
10	36.83843702848047	39.0	37.0	39.0	32.0	39.0
11	36.727581232624594	39.0	37.0	39.0	32.0	39.0
12	36.61267555034792	39.0	35.0	39.0	32.0	39.0
13	36.45225251615257	39.0	35.0	39.0	32.0	39.0
14	37.56866706092378	40.0	36.0	41.0	32.0	41.0
15	37.69258308415915	40.0	36.0	41.0	33.0	41.0
16	37.72170191951628	40.0	36.0	41.0	33.0	41.0
17	37.7093643433132	40.0	36.0	41.0	33.0	41.0
18	37.67983789400379	39.0	36.0	41.0	33.0	41.0
19	37.659259607253595	39.0	36.0	41.0	33.0	41.0
20	37.588792136228726	39.0	36.0	41.0	33.0	41.0
21	37.48971154749601	39.0	35.0	41.0	32.0	41.0
22	37.51217453062217	39.0	35.0	41.0	32.0	41.0
23	37.41756249620021	39.0	35.0	41.0	32.0	41.0
24	37.39729095622088	39.0	35.0	41.0	32.0	41.0
25	37.25535839629472	39.0	35.0	41.0	32.0	41.0
26	37.23079101978102	39.0	35.0	41.0	32.0	41.0
27	37.01565375750977	39.0	35.0	41.0	31.0	41.0
28	36.98601538708126	39.0	35.0	41.0	31.0	41.0
29	36.9833859316535	39.0	35.0	41.0	31.0	41.0
30	36.84829161393017	39.0	35.0	41.0	31.0	41.0
31	36.80076659057873	39.0	35.0	41.0	31.0	41.0
32	36.67296096301905	39.0	35.0	41.0	31.0	41.0
33	36.54347099170402	39.0	35.0	40.0	30.0	41.0
34	36.4594389573926	39.0	35.0	40.0	30.0	41.0
35	36.37793136610089	39.0	35.0	40.0	30.0	41.0
36	36.28218906655023	38.0	35.0	40.0	30.0	41.0
37	36.18425394211053	38.0	35.0	40.0	30.0	41.0
38	36.09091725574943	38.0	35.0	40.0	30.0	41.0
39	36.10381858277465	38.0	35.0	40.0	30.0	41.0
40	36.07245856155109	38.0	35.0	40.0	30.0	41.0
41	35.994218790588675	38.0	35.0	40.0	30.0	41.0
42	35.92566641426842	38.0	35.0	40.0	30.0	41.0
43	35.84347624232442	38.0	35.0	40.0	29.0	41.0
44	35.74527858686461	38.0	35.0	40.0	29.0	41.0
45	35.69985104818964	38.0	34.0	40.0	29.0	41.0
46	35.60990377547242	38.0	34.0	40.0	29.0	41.0
47	35.52956928331795	37.0	34.0	40.0	29.0	41.0
48	35.45164040435304	37.0	34.0	40.0	28.0	41.0
49	35.37489844194748	37.0	34.0	40.0	28.0	41.0
50	35.30921456245751	37.0	34.0	40.0	28.0	41.0
51	34.59133868712382	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	31.0
10	45.0
11	41.0
12	33.0
13	31.0
14	39.0
15	66.0
16	96.0
17	178.0
18	301.0
19	488.0
20	853.0
21	1248.0
22	1842.0
23	2645.0
24	3604.0
25	4817.0
26	6188.0
27	7621.0
28	9513.0
29	11555.0
30	14456.0
31	18581.0
32	24294.0
33	33160.0
34	53843.0
35	67172.0
36	67038.0
37	93205.0
38	136352.0
39	164309.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.24514317612792	21.44919886586599	24.064007826187883	13.241650131818206
2	31.854823109362133	24.020759294979854	25.717815078676402	18.406602516981614
3	31.460473882308726	23.724928287579242	27.478016481421097	17.336581348690938
4	29.63215258855586	26.046946073364985	25.692805544655144	18.628095793424013
5	24.722822512449497	30.989852485201542	24.73456732124401	19.55275768110495
6	24.743824994058507	35.71264736280681	23.71815775074476	15.825369892389919
7	81.65377961764429	6.023152472489512	7.718550165532717	4.604517744333475
8	83.4685598377282	4.823662059016973	6.585107029751673	5.122671073503159
9	77.99147188707298	7.312456129684797	9.108997352581923	5.5870746306603065
10	42.595934361718	24.28163222443915	16.459313218851385	16.66312019499146
11	30.65533269588959	26.483162089415302	22.403430036864883	20.458075177830224
12	27.609558339919637	22.559290558279123	28.904941662843846	20.926209438957393
13	25.035648949046873	24.565442074603023	29.147990117779703	21.250918858570394
14	21.397383532949025	26.74707485173906	28.544030597299525	23.31151101801239
15	20.494691346424883	25.74945697531103	31.65889206382544	22.096959614438653
16	22.936091659251318	23.405469488368492	31.113656587317816	22.54478226506237
17	22.118376618711	23.277105636955525	27.543096539564804	27.06142120476867
18	23.04497294548751	24.254273728658994	29.159458578131996	23.54129474772151
19	24.74410134250073	25.79353455184573	26.025390894871524	23.43697321078201
20	25.95948179140114	25.119382527040695	25.849909634059394	23.07122604749877
21	25.220111534231282	26.414074978859343	25.68092256163952	22.684890925269855
22	23.013331048852876	25.23945592518695	25.339632235493088	26.407580790467083
23	22.946592900055823	26.846560290939642	25.2956928331795	24.911153975825037
24	23.525819234956973	25.427511040120265	27.228059315429636	23.81861040949312
25	23.610243684056353	25.75982004189442	25.508757482134076	25.12117879191515
26	22.39969933289486	27.160215772863687	25.786625840790133	24.653459053451314
27	23.91381244783923	25.486787780977277	26.604479055551565	23.994920715631928
28	21.742266388844367	26.424990742327186	28.376148918648546	23.4565939501799
29	24.099242252571422	24.947631970198582	26.84642211671853	24.106703660511467
30	25.27897375242496	24.96531827050091	26.123909114524324	23.63179886254981
31	25.288922296345017	26.06767220653177	24.374899823689695	24.26850567343352
32	25.95989631406448	26.89934284340439	24.175928945288536	22.964831897242597
33	24.8933295013016	25.86358888194947	24.49082799520259	24.752253621546334
34	23.19889902780618	25.13927961488081	27.544063759112593	24.117757598200416
35	24.190437238505286	24.55494083379852	26.394592413682567	24.86002951401363
36	25.696259900182945	25.84410631677269	26.1943779672914	22.265255815752965
37	23.17002061559379	27.57349486820943	27.47608204232553	21.780402473871256
38	22.768762677484787	27.728940866960333	26.14670786100779	23.35558859454709
39	23.373136720628306	25.790080196317934	27.607900249266294	23.228882833787466
40	24.2613206139357	23.818334061050898	28.779064947410895	23.14128037760251
41	22.024556322576007	24.453382781281263	27.06155937898978	26.460501517152945
42	23.444434618722056	25.474628449519432	25.870912115668403	25.210024816090108
43	22.965660942569265	24.938097948941863	27.49072850976339	24.605512598725483
44	23.111849268505676	24.974299594873184	27.010434917178372	24.90341621944277
45	23.332789848063626	24.457251659472394	27.175967634070446	25.03399085839353
46	23.588550331341782	24.789837009688775	27.40575136377956	24.215861295189878
47	22.488821705512045	25.046150189851378	28.34409249935058	24.120935605285993
48	22.290541698216447	24.276657952479123	28.235211213114393	25.19758913619004
49	22.768486329042563	24.650695569029075	28.43929453769669	24.14152356423167
50	21.79739790306802	23.762511675721683	29.169407122052053	25.270683299158243
51	21.690451055927397	23.646445329987674	26.720683575506687	27.94242003857824
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1383.0
1	1497.0
2	1611.0
3	2164.0
4	2717.0
5	2063.5
6	1410.0
7	1386.0
8	1362.0
9	1453.5
10	1545.0
11	1582.0
12	1619.0
13	1646.0
14	1673.0
15	1670.5
16	1668.0
17	1567.5
18	1467.0
19	1445.5
20	1424.0
21	1445.5
22	1467.0
23	1838.5
24	2210.0
25	2656.0
26	4133.5
27	5165.0
28	6052.0
29	6939.0
30	7580.0
31	8221.0
32	9958.5
33	11696.0
34	13628.5
35	15561.0
36	16862.5
37	18164.0
38	19559.5
39	20955.0
40	24718.5
41	28482.0
42	34099.5
43	39717.0
44	44870.5
45	50024.0
46	57727.5
47	65431.0
48	65558.0
49	65685.0
50	60204.5
51	54724.0
52	49885.5
53	45047.0
54	43780.0
55	42513.0
56	40127.5
57	37742.0
58	35766.0
59	33790.0
60	33667.0
61	33544.0
62	31294.0
63	29044.0
64	26419.0
65	23794.0
66	20565.0
67	17336.0
68	15056.5
69	12777.0
70	11542.0
71	10307.0
72	9106.5
73	7906.0
74	6776.5
75	4783.5
76	3920.0
77	3018.0
78	2116.0
79	1785.5
80	1455.0
81	1120.5
82	786.0
83	572.0
84	358.0
85	255.0
86	152.0
87	87.5
88	23.0
89	14.0
90	5.0
91	8.5
92	12.0
93	20.0
94	28.0
95	14.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	723724.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.060397651146882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.83083979202453	20.24952281341449
2	10.229871942956674	5.536488053934363
3	3.4796681792631916	2.8248361387471266
4	1.7234211203953458	1.8654584335313256
5	0.9827607883032493	1.329694886372025
6	0.6720057624107595	1.0910845892818373
7	0.48847599665361563	0.9252848298740993
8	0.4068325899347667	0.880724132886461
9	0.31108601230407434	0.7576300076992042
>10	5.674597181053307	41.14758593422518
>50	1.1474875788656997	19.199463466234114
>100	0.04678376777632161	2.5079070475100647
>500	0.003598751367409354	0.6556701567046616
>1k	0.002570536691006682	1.0286495095850345
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1969	0.27206504136936177	No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1718	0.23738331187027095	No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	1376	0.19012772824999585	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1233	0.17036881463099193	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1098	0.15171529478088333	No Hit
TCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	809	0.11178294487953971	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCC	772	0.10667049869839884	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	748	0.10335431739171286	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3817422111191558E-4	0.0	0.0	0.2028397565922921	0.0
2	1.3817422111191558E-4	0.0	0.0	0.9706739033112071	0.0
3	1.3817422111191558E-4	0.0	0.0	1.309615267698736	0.0
4	1.3817422111191558E-4	0.0	0.0	1.682685664700908	0.0
5	1.3817422111191558E-4	0.0	0.0	2.643411024092057	0.0
6	1.3817422111191558E-4	0.0	0.0	3.398947665132012	0.0
7	1.3817422111191558E-4	0.0	0.0	4.1108212523006005	0.0
8	1.3817422111191558E-4	0.0	0.0	5.350105841453372	0.0
9	1.3817422111191558E-4	0.0	0.0	5.901420983689915	0.0
10	1.3817422111191558E-4	0.0	0.0	7.069960371633385	0.0
11	1.3817422111191558E-4	0.0	0.0	8.618478867634623	0.0
12	1.3817422111191558E-4	0.0	0.0	9.689052732809746	0.0
13	1.3817422111191558E-4	0.0	0.0	10.216463734793926	0.0
14	1.3817422111191558E-4	0.0	0.0	10.372186081987056	0.0
15	1.3817422111191558E-4	0.0	0.0	10.703113341550093	0.0
16	2.7634844222383117E-4	0.0	0.0	11.305000248713599	0.0
17	2.7634844222383117E-4	0.0	0.0	12.023920721158895	0.0
18	2.7634844222383117E-4	0.0	0.0	12.87756105918831	0.0
19	2.7634844222383117E-4	0.0	0.0	13.34569532031548	0.0
20	2.7634844222383117E-4	0.0	0.0	13.779424200385783	0.0
21	2.7634844222383117E-4	0.0	0.0	14.281411145685372	0.0
22	2.7634844222383117E-4	0.0	0.0	14.834660727017482	0.0
23	5.526968844476623E-4	0.0	0.0	15.368151394730589	0.0
24	8.290453266714935E-4	0.0	0.0	15.792898950428617	0.0
25	0.0012435679900072404	0.0	0.0	16.165002127883007	0.0
26	0.0012435679900072404	0.0	0.0	16.502561750059414	0.0
27	0.0012435679900072404	0.0	0.0	16.865545428920417	0.0
28	0.0012435679900072404	0.0	0.0	17.227423714012524	0.0
29	0.0012435679900072404	0.0	0.0	17.57534640277233	0.0
30	0.0012435679900072404	0.0	0.0	18.004239185103714	0.0
31	0.0012435679900072404	0.0	0.0	18.359899630245785	0.0
32	0.0012435679900072404	0.0	0.0	18.72385052865457	0.0
33	0.0015199164322310715	0.0	0.0	19.08103089022887	0.0
34	0.0015199164322310715	0.0	0.0	19.429920798536457	0.0
35	0.0015199164322310715	0.0	0.0	19.814321481669808	0.0
36	0.0015199164322310715	0.0	0.0	20.173850805003013	0.0
37	0.0015199164322310715	0.0	0.0	20.540703362055147	0.0
38	0.0015199164322310715	0.0	0.0	20.891389535237188	0.0
39	0.0015199164322310715	0.0	0.0	21.24000309510255	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAACGT	30	2.1643827E-6	45.000004	28
ACTATCC	20	7.0315535E-4	45.0	34
TCCGATT	20	7.0315535E-4	45.0	15
TAGCGAA	20	7.0315535E-4	45.0	1
TCGATAG	25	3.8893682E-5	45.0	1
GGTATGC	105	0.0	45.0	8
GTCGTAA	20	7.0315535E-4	45.0	31
TCGTAAG	25	3.8893682E-5	45.0	1
CGGTCTA	25	3.8893682E-5	45.0	31
ATAACCG	20	7.0315535E-4	45.0	15
ACGTAGA	20	7.0315535E-4	45.0	44
ATACGCG	20	7.0315535E-4	45.0	1
TGCGTAT	20	7.0315535E-4	45.0	10
ACGGTCT	20	7.0315535E-4	45.0	30
TACGAAT	145	0.0	41.896553	12
TTGCGAG	70	0.0	41.785713	1
GCTACGA	150	0.0	40.5	10
CAACGTC	45	1.9266736E-8	40.0	20
GTATGCG	45	1.9266736E-8	40.0	1
AGTTACG	45	1.9266736E-8	40.0	1
>>END_MODULE