FastQCFastQC Report
Sat 18 Jun 2016
SRR3552557_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552557_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940728
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC30870.3281501135290966No Hit
CCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC17910.19038446819909688No Hit
CTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGCT14760.15689976273694414No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11360.12075754096827138No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGC9470.10066671769097975No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATT1500.045.00000417
TTATCGA207.032632E-445.00000412
TAGCCGT302.1650794E-645.00000444
TATTGCG600.045.0000041
AATCTAC207.032632E-445.00000428
TTAACGG650.045.0000042
ACGTAAT207.032632E-445.00000411
ACGTAAG406.8121153E-945.0000041
TATCGCG207.032632E-445.00000419
CGCGTAC253.890263E-545.043
TCCGGTA253.890263E-545.018
TGCGTAT1550.042.09677511
GATGCGT1500.042.0000049
TACGAAG603.6379788E-1241.2500041
CGTAAGG1800.041.252
TCGCAAT556.002665E-1140.90909222
TGTTGCG501.0804797E-940.51
GCGATAT950.040.2631579
TCAAGCG5600.040.17857417
CGCAAGG2650.039.9056632