##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552557_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	940728
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.593979343657253	31.0	31.0	34.0	30.0	34.0
2	31.715694653502393	31.0	31.0	34.0	30.0	34.0
3	31.713354976146135	31.0	31.0	34.0	30.0	34.0
4	35.433819339915466	37.0	35.0	37.0	33.0	37.0
5	35.356058286773646	37.0	35.0	37.0	33.0	37.0
6	35.35202098800078	37.0	35.0	37.0	33.0	37.0
7	35.77972910341778	37.0	35.0	37.0	35.0	37.0
8	35.716777857148934	37.0	35.0	37.0	35.0	37.0
9	37.320357212711855	39.0	37.0	39.0	35.0	39.0
10	36.43635461047189	38.0	35.0	39.0	32.0	39.0
11	36.51594509783912	38.0	35.0	39.0	32.0	39.0
12	36.376823056186275	38.0	35.0	39.0	32.0	39.0
13	35.96953529606858	38.0	35.0	39.0	31.0	39.0
14	35.84995450332083	39.0	35.0	40.0	29.0	41.0
15	36.58387546665986	39.0	35.0	40.0	30.0	41.0
16	37.02238798037265	39.0	35.0	40.0	32.0	41.0
17	37.193421477834185	39.0	35.0	40.0	32.0	41.0
18	37.246365580699205	39.0	36.0	40.0	32.0	41.0
19	37.223776692093786	39.0	35.0	40.0	32.0	41.0
20	37.01772563376449	39.0	35.0	40.0	32.0	41.0
21	36.99240375538945	39.0	35.0	40.0	31.0	41.0
22	37.011113733193866	39.0	35.0	40.0	31.0	41.0
23	36.60403219634156	39.0	35.0	40.0	31.0	41.0
24	34.97284124635389	38.0	34.0	40.0	27.0	41.0
25	35.81617003001931	38.0	35.0	40.0	27.0	41.0
26	36.27168745907425	38.0	35.0	40.0	30.0	41.0
27	32.33041963245486	38.0	33.0	40.0	0.0	41.0
28	33.933677960047966	38.0	33.0	40.0	16.0	41.0
29	35.22364806830455	38.0	33.0	40.0	25.0	41.0
30	35.88213915180584	38.0	34.0	40.0	30.0	41.0
31	36.10388443843492	38.0	35.0	40.0	30.0	41.0
32	33.093384060004595	38.0	33.0	40.0	12.0	41.0
33	33.82176888537388	38.0	33.0	40.0	16.0	41.0
34	34.76095959724809	38.0	33.0	40.0	24.0	41.0
35	35.263998732896226	38.0	34.0	40.0	27.0	41.0
36	35.42883490233096	38.0	34.0	40.0	28.0	41.0
37	35.580362230102644	38.0	34.0	40.0	29.0	41.0
38	35.58396475920776	38.0	34.0	40.0	29.0	41.0
39	35.588880101368304	38.0	34.0	40.0	29.0	41.0
40	35.42299261848271	38.0	34.0	40.0	28.0	41.0
41	35.36682760585419	38.0	34.0	40.0	28.0	41.0
42	35.24714795349984	38.0	34.0	40.0	27.0	41.0
43	35.18704450170506	37.0	34.0	40.0	27.0	41.0
44	35.04881857455077	37.0	34.0	40.0	27.0	41.0
45	34.94891403253651	37.0	34.0	40.0	26.0	41.0
46	34.78354848585351	37.0	33.0	40.0	26.0	41.0
47	34.67907407879855	37.0	33.0	40.0	26.0	41.0
48	34.62101691455979	36.0	33.0	40.0	26.0	41.0
49	34.56454150402667	36.0	33.0	40.0	26.0	41.0
50	34.38372622054409	36.0	33.0	40.0	26.0	41.0
51	32.97893652575452	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	23.0
9	42.0
10	53.0
11	47.0
12	29.0
13	22.0
14	37.0
15	44.0
16	72.0
17	179.0
18	424.0
19	755.0
20	1385.0
21	2179.0
22	3394.0
23	4786.0
24	6542.0
25	8790.0
26	11464.0
27	14266.0
28	18008.0
29	22999.0
30	30010.0
31	37676.0
32	47095.0
33	61824.0
34	87952.0
35	94770.0
36	87327.0
37	110146.0
38	148902.0
39	139465.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.72710921754216	18.32346863280353	23.50116080312269	11.448261346531623
2	30.664761758978155	23.729707205483415	26.30558461106717	19.29994642447126
3	32.445191383694336	24.811422642889337	26.469181314896552	16.274204658519782
4	27.75031677594374	26.630120502419402	25.5638186595913	20.055744062045562
5	24.971830327150887	30.34065107023497	24.890616628823633	19.79690197379051
6	24.214544480444932	35.72733032289886	24.5492852344142	15.508839962242007
7	81.96460613482324	5.5849299691302905	7.687344269544438	4.763119626502029
8	82.82532251617896	4.516502113256967	7.115659361685843	5.542516008878231
9	76.81869785953006	6.60243981257069	10.135023088501672	6.4438392393975725
10	40.92851493736765	26.04769922868247	16.43429344082455	16.589492393125322
11	34.159395702052024	25.997100118206323	21.37738007160412	18.466124108137528
12	28.42128649301392	23.151963160445952	27.640189300201545	20.78656104633858
13	25.899409818778647	25.598153770271537	29.72346948320875	18.77896692774107
14	21.390242450527676	30.218617921439566	27.455863969181316	20.93527565885144
15	20.90072794686668	23.46416817613593	34.13239533637778	21.502708540619604
16	23.49605837181417	25.029976783937546	29.979866656461805	21.49409818778648
17	22.88663673240299	23.86938626255411	27.51369152401119	25.73028548103171
18	27.261121174239527	22.601113180430474	28.366860559056388	21.770905086273608
19	24.950144994089683	26.46216547184734	26.33407318587307	22.25361634818991
20	27.093485045624238	26.10478267894652	25.87177164919084	20.929960626238405
21	23.81506662924884	28.898257519708142	26.932652158753644	20.354023692289374
22	23.604272435815666	24.445429497155395	26.360542048286007	25.589756018742932
23	20.914440731008323	28.407998911481318	26.682420423331717	23.995139934178635
24	20.99958755346923	26.6748730770212	27.936236616747877	24.38930275276169
25	20.519852709816227	26.469393916201067	27.609362111045915	25.40139126293679
26	19.802217006403552	26.682101521374936	28.782496109396128	24.733185362825385
27	19.43633016132187	34.4548052146848	24.151189291697495	21.95767533229584
28	17.913679618338136	29.780340332168276	28.008095857676185	24.297884191817403
29	21.23568130214047	27.718426580265497	26.79913853951408	24.246753578079954
30	24.25632063678343	26.363837368506093	26.786595062547303	22.593246932163176
31	25.357063890944037	28.83330782117679	22.77757226318341	23.03205602469577
32	22.137429735268853	33.63735319879923	21.80651580478098	22.41870126115094
33	23.556118240341522	28.019363726815826	22.599306069342042	25.82521196350061
34	19.91308858671157	29.936814892296184	27.14238334566421	23.007713175328043
35	19.339171365155497	29.81924637089573	24.9916022484714	25.849980015477374
36	23.14133309521987	33.76246906651019	22.222045054468452	20.87415278380148
37	21.709463309266866	31.320424182122782	22.423272189198155	24.5468403194122
38	22.621841807621333	30.263476796693624	22.020392717129713	25.094288678555333
39	25.76727810801847	25.07961918854334	22.575388422583362	26.577714280854824
40	25.44306111862302	24.662814331028734	24.751469075014242	25.142655475333996
41	20.548766487231166	27.14365895349134	22.921609646996792	29.385964912280706
42	23.466506790485667	26.48385080490854	23.66433230434302	26.385310100262778
43	23.020681854903863	25.058996862004747	25.04220135894754	26.878119924143856
44	23.172372885680026	25.03422881002798	24.968960209539844	26.82443809475215
45	23.359993536920342	24.619443664906328	25.382150844877582	26.638411953295744
46	24.254088323085952	26.09064469219583	24.276517760712977	25.378749224005237
47	20.27153438613499	27.451718243743144	27.003342092507083	25.273405277614785
48	22.0212431223478	25.042945463513366	27.11091835259501	25.824893061543825
49	25.348878740719954	22.446552032043268	27.685473378064646	24.519095849172132
50	21.359734163328824	23.533050998800928	27.50922689661624	27.59798794125401
51	20.97992193280098	22.649905179818184	24.85043498226905	31.519737905111782
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	437.0
1	641.0
2	845.0
3	1865.0
4	2885.0
5	2129.5
6	1374.0
7	1377.5
8	1381.0
9	1469.0
10	1557.0
11	1711.0
12	1865.0
13	1918.0
14	1971.0
15	2035.0
16	2099.0
17	2091.5
18	2084.0
19	2049.0
20	2014.0
21	2245.5
22	2477.0
23	2397.0
24	2317.0
25	2924.5
26	3704.0
27	3876.0
28	5116.5
29	6357.0
30	7667.5
31	8978.0
32	9860.0
33	10742.0
34	12517.0
35	14292.0
36	16027.5
37	17763.0
38	19905.5
39	22048.0
40	27545.0
41	33042.0
42	39506.5
43	45971.0
44	56528.0
45	67085.0
46	77946.0
47	88807.0
48	98923.0
49	109039.0
50	109615.0
51	110191.0
52	98591.5
53	86992.0
54	77945.0
55	68898.0
56	60764.5
57	52631.0
58	47635.5
59	42640.0
60	39528.5
61	36417.0
62	32292.5
63	28168.0
64	23823.0
65	19478.0
66	16378.0
67	13278.0
68	11627.5
69	9977.0
70	8067.0
71	6157.0
72	5371.5
73	4586.0
74	3569.0
75	2255.5
76	1959.0
77	1430.0
78	901.0
79	714.0
80	527.0
81	376.5
82	226.0
83	181.5
84	137.0
85	111.5
86	86.0
87	57.0
88	28.0
89	35.5
90	43.0
91	28.5
92	14.0
93	8.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	940728.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.5808324703658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.69077776807723	29.202132166218757
2	10.795898659636698	8.330295151089803
3	4.1975902120035835	4.858395742456727
4	2.0970782977318123	3.236281059281238
5	1.2099584079639287	2.334060131688343
6	0.7498114810869849	1.735701068170429
7	0.532690748816172	1.4386156777013308
8	0.39261123551597815	1.2117814642740228
9	0.30127864036360863	1.0461222675691175
>10	3.660837609255714	33.880764999481514
>50	0.3191397073113019	7.578060260887333
>100	0.04686449920126103	3.154363569821593
>500	0.004312683975536497	1.1626009978238345
>1k	0.0011500490601430659	0.8308254435359718
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC	3087	0.3281501135290966	No Hit
CCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC	1791	0.19038446819909688	No Hit
CTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGCT	1476	0.15689976273694414	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1136	0.12075754096827138	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGC	947	0.10066671769097975	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0630065226080228E-4	0.0	0.0	0.20909338299699806	0.0
2	1.0630065226080228E-4	0.0	0.0	1.1044637769897356	0.0
3	1.0630065226080228E-4	0.0	0.0	1.513402386237042	0.0
4	1.0630065226080228E-4	0.0	0.0	1.989204105756393	0.0
5	1.0630065226080228E-4	0.0	0.0	3.35484858535092	0.0
6	1.0630065226080228E-4	0.0	0.0	4.248092966298441	0.0
7	1.0630065226080228E-4	0.0	0.0	5.167699909006641	0.0
8	1.0630065226080228E-4	0.0	0.0	7.062934238164486	0.0
9	1.0630065226080228E-4	0.0	0.0	7.878579142961621	0.0
10	1.0630065226080228E-4	0.0	0.0	9.803790256057011	0.0
11	1.0630065226080228E-4	0.0	0.0	11.965201418475903	0.0
12	1.0630065226080228E-4	0.0	0.0	13.854376610454882	0.0
13	1.0630065226080228E-4	0.0	0.0	14.623461829561785	0.0
14	1.0630065226080228E-4	0.0	0.0	14.916957930453862	0.0
15	1.0630065226080228E-4	0.0	0.0	15.502993426367665	0.0
16	1.0630065226080228E-4	0.0	0.0	16.633713464465817	0.0
17	1.0630065226080228E-4	0.0	0.0	17.907407879854752	0.0
18	1.0630065226080228E-4	0.0	0.0	19.64021481235809	0.0
19	1.0630065226080228E-4	0.0	0.0	20.39314233232135	0.0
20	1.0630065226080228E-4	0.0	0.0	21.17190091078399	0.0
21	1.0630065226080228E-4	0.0	0.0	22.007636638858415	0.0
22	2.1260130452160455E-4	0.0	0.0	22.851238615200142	0.0
23	0.0010630065226080226	0.0	0.0	23.6858050360997	0.0
24	0.0013819084793904295	0.0	0.0	24.34540058337798	0.0
25	0.0018071110884336386	0.0	0.0	24.93377469364152	0.0
26	0.0018071110884336386	0.0	0.0	25.469423680383702	0.0
27	0.0018071110884336386	0.0	0.0	26.056734784124636	0.0
28	0.0018071110884336386	0.0	0.0	26.640963168950005	0.0
29	0.0018071110884336386	0.0	0.0	27.242731161398407	0.0
30	0.0018071110884336386	0.0	0.0	28.028505582910256	0.0
31	0.0018071110884336386	0.0	0.0	28.612415065778844	0.0
32	0.0018071110884336386	0.0	0.0	29.23990781607436	0.0
33	0.0018071110884336386	0.0	0.0	29.89301902356473	0.0
34	0.001913411740694441	0.0	0.0	30.54379161670536	0.0
35	0.001913411740694441	0.0	0.0	31.188505072667127	0.0
36	0.0021260130452160453	0.0	0.0	31.780387104455272	0.0
37	0.0021260130452160453	0.0	0.0	32.42169893954469	0.0
38	0.0022323136974768476	0.0	0.0	33.10064120553444	0.0
39	0.0022323136974768476	0.0	0.0	33.84846629418918	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATT	150	0.0	45.000004	17
TTATCGA	20	7.032632E-4	45.000004	12
TAGCCGT	30	2.1650794E-6	45.000004	44
TATTGCG	60	0.0	45.000004	1
AATCTAC	20	7.032632E-4	45.000004	28
TTAACGG	65	0.0	45.000004	2
ACGTAAT	20	7.032632E-4	45.000004	11
ACGTAAG	40	6.8121153E-9	45.000004	1
TATCGCG	20	7.032632E-4	45.000004	19
CGCGTAC	25	3.890263E-5	45.0	43
TCCGGTA	25	3.890263E-5	45.0	18
TGCGTAT	155	0.0	42.096775	11
GATGCGT	150	0.0	42.000004	9
TACGAAG	60	3.6379788E-12	41.250004	1
CGTAAGG	180	0.0	41.25	2
TCGCAAT	55	6.002665E-11	40.909092	22
TGTTGCG	50	1.0804797E-9	40.5	1
GCGATAT	95	0.0	40.263157	9
TCAAGCG	560	0.0	40.178574	17
CGCAAGG	265	0.0	39.905663	2
>>END_MODULE