FastQCFastQC Report
Sat 18 Jun 2016
SRR3552554_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552554_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428716
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC40810.28564109312137614No Hit
TGCGGAGGGGATGATGGATCCAAAAAGTTTGGAAAGGTTGTTTACAAAGCT25810.18065171804613372No Hit
GCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC24930.17449234137505285No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCC21290.14901491969012737No Hit
TCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC20950.14663516052175518No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGT207.033863E-445.011
CCTCGAC207.033863E-445.045
TCTACGC1050.045.014
CTACGCG351.2122109E-745.01
CGTAAGC207.033863E-445.025
CGGCTAT207.033863E-445.017
CCAACGA207.033863E-445.033
GTACGAG351.2122109E-745.01
GCCGACG302.1658743E-644.9999961
AATTCGC302.1658743E-644.99999613
TTGCGCG1850.042.5675661
GGCGCGA700.041.7857138
TGCGGAG5150.041.5048561
CGCCCTA403.4590084E-739.37517
GCGCGAG1200.039.3749961
CTACGCA1150.039.13043215
GCGCGAC750.039.0000049
GCGTAAG750.039.0000041
AGTCCGG356.249358E-638.5714262
ACGTCGG356.249358E-638.5714262
Produced by FastQC (version 0.11.5)