FastQCFastQC Report
Sat 18 Jun 2016
SRR3552549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1877103
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC40400.21522527000383038TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC26690.14218718951490675TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT20880.1112352385564351TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAA302.16624E-645.00000433
CGAATCC301.1401972E-437.50000427
ACGGTCG301.1401972E-437.50000442
TCAAGCG11650.037.27467717
AACGCAT2000.037.12527
ATTCGCG1100.036.818181
TCGCCGA250.002107447536.045
ACGATTG504.8818038E-836.01
CGAGACA10650.035.91549322
CACAACG4450.035.89887612
ACACGAC10350.035.86956426
TAGGGCG9800.035.8163265
CGTAAGG3150.035.7142872
AAACACG11000.035.59090840
TAACGCA2100.035.35714326
GCTACGA2050.035.12195210
CGACCAA10450.035.09569529
AGGGCGA21250.034.9411776
GTCAAGC12650.034.8616616
AACGAGC11250.034.815
Produced by FastQC (version 0.11.5)