FastQCFastQC Report
Sat 18 Jun 2016
SRR3552548_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552548_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647962
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC24550.148971881633193No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC18540.11250259411321377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGT302.166078E-645.00000415
TCTAGCG406.8175723E-945.01
CGACGGT750.042.028
AACACGT6700.039.62686541
CACGTGA6800.038.71323443
ACACGTG6850.038.4306642
ACACGAC6950.037.87769726
AAACACG7300.037.60273740
CCGTATA301.1401368E-437.50000434
CGTAAGG3900.037.52
CGTTAGG3250.037.3846172
CGTATGG2000.037.1250042
ACGTGAG7150.036.8181844
CGTACTC552.750312E-936.8181845
CAATGCG552.750312E-936.818181
GACACGA7300.036.3698625
CGACCAA7450.036.2416129
CACGACC7100.036.12676227
CTCGTAG250.00210737336.0000041
TCAAGCG8400.035.89285717
Produced by FastQC (version 0.11.5)