##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552548_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1647962
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.655594000347094	31.0	31.0	34.0	30.0	34.0
2	31.783763824651295	31.0	31.0	34.0	30.0	34.0
3	31.785338496882815	31.0	31.0	34.0	30.0	34.0
4	35.48265008537818	37.0	35.0	37.0	33.0	37.0
5	35.391748110696724	37.0	35.0	37.0	33.0	37.0
6	35.38211560703463	37.0	35.0	37.0	33.0	37.0
7	35.81079357412368	37.0	35.0	37.0	35.0	37.0
8	35.75060043860235	37.0	35.0	37.0	35.0	37.0
9	37.36343253060447	39.0	37.0	39.0	35.0	39.0
10	36.41127829403833	38.0	35.0	39.0	32.0	39.0
11	36.52117524554571	38.0	35.0	39.0	32.0	39.0
12	36.51704468913725	38.0	35.0	39.0	32.0	39.0
13	36.22442750500315	38.0	35.0	39.0	32.0	39.0
14	36.17828020306293	39.0	36.0	41.0	30.0	41.0
15	36.88197543389957	39.0	36.0	41.0	31.0	41.0
16	37.29340239641448	39.0	36.0	41.0	32.0	41.0
17	37.4514794637255	39.0	36.0	41.0	32.0	41.0
18	37.49175405743579	39.0	36.0	41.0	32.0	41.0
19	37.46914856046438	39.0	36.0	41.0	32.0	41.0
20	37.29669070039236	39.0	36.0	41.0	32.0	41.0
21	37.29550863430104	39.0	36.0	41.0	32.0	41.0
22	37.29832605363473	39.0	36.0	41.0	32.0	41.0
23	36.9048970789375	39.0	35.0	40.0	31.0	41.0
24	35.30494999277896	39.0	35.0	40.0	27.0	41.0
25	36.11965506486193	39.0	35.0	40.0	27.0	41.0
26	36.552086152471965	39.0	35.0	40.0	30.0	41.0
27	32.77450936368678	38.0	33.0	40.0	0.0	41.0
28	34.335042312868865	38.0	33.0	40.0	16.0	41.0
29	35.589623425782875	38.0	34.0	40.0	26.0	41.0
30	36.20850662818682	38.0	35.0	40.0	30.0	41.0
31	36.461500932667136	38.0	35.0	40.0	30.0	41.0
32	33.64943366412575	38.0	34.0	40.0	15.0	41.0
33	34.43004935793422	38.0	34.0	40.0	16.0	41.0
34	35.43083214297417	38.0	34.0	40.0	25.0	41.0
35	35.85664293230062	38.0	34.0	40.0	29.0	41.0
36	36.06321869072224	38.0	35.0	40.0	30.0	41.0
37	36.229802022134	38.0	35.0	40.0	30.0	41.0
38	36.230728014359556	38.0	35.0	40.0	30.0	41.0
39	36.261557608731266	38.0	35.0	40.0	30.0	41.0
40	36.15245254441547	38.0	35.0	40.0	30.0	41.0
41	36.04824989896612	38.0	35.0	40.0	30.0	41.0
42	35.952779250977876	38.0	35.0	40.0	29.0	41.0
43	35.99303199952426	38.0	35.0	40.0	29.0	41.0
44	35.923646297669485	38.0	35.0	40.0	29.0	41.0
45	35.84923560130634	38.0	35.0	40.0	29.0	41.0
46	35.68878408604082	38.0	34.0	40.0	29.0	41.0
47	35.58352012971173	38.0	34.0	40.0	28.0	41.0
48	35.501680257190394	38.0	34.0	40.0	28.0	41.0
49	35.4727754644828	38.0	34.0	40.0	28.0	41.0
50	35.35396386567166	37.0	34.0	40.0	28.0	41.0
51	34.0882380783052	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	49.0
9	93.0
10	111.0
11	85.0
12	67.0
13	42.0
14	60.0
15	100.0
16	134.0
17	313.0
18	544.0
19	1014.0
20	1940.0
21	2999.0
22	4345.0
23	6424.0
24	9223.0
25	12594.0
26	16433.0
27	21299.0
28	27362.0
29	35289.0
30	45622.0
31	58194.0
32	74497.0
33	97836.0
34	137655.0
35	148166.0
36	157260.0
37	198996.0
38	281878.0
39	307283.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.3827212035229	19.423142038469337	22.14177268650612	13.052364071501648
2	31.763717852717477	23.887201282553846	25.702170317034007	18.646910547694667
3	33.37874295645167	24.849966200676956	24.696018476154183	17.075272366717194
4	29.269485582798634	26.35418777860169	24.734550918043013	19.641775720556666
5	24.630118898372658	30.776741211265797	24.278957888592092	20.31418200176946
6	24.65263155339747	36.54944713531016	23.89205576342173	14.905865547870642
7	84.28197980293236	5.387806272232005	6.351481405517847	3.9787325193178
8	86.15508124580542	4.1877786016910585	5.585322962544039	4.071817189959477
9	80.24760279666643	6.252692719856405	8.118512441427654	5.381192042049513
10	38.76448607431482	25.90478421225732	17.93645727267983	17.394272440748026
11	31.182879216875147	27.59784509594275	21.17445669256937	20.04481899461274
12	26.03433817041898	24.40098740140853	28.086266552262735	21.47840787590976
13	25.70902727125989	24.3813267538936	29.836488948167496	20.073157026679013
14	22.552037000853176	29.257895509726563	27.071740731885807	21.118326757534458
15	22.63140776304308	26.43052449024917	30.14250328587674	20.795564460831013
16	25.18990122345054	25.026790666289635	28.460243622122356	21.32306448813747
17	24.42780840820359	24.682790015789198	27.106996399188816	23.782405176818397
18	25.76503584427311	24.520953759856113	27.828250894134694	21.885759501736086
19	25.038077334307467	27.6270326621609	25.71169723573723	21.623192767794404
20	25.88130066105893	26.768396358653902	25.95551353732671	21.39478944296046
21	25.39645938437901	27.312219577878615	26.723492410625973	20.567828627116402
22	23.824760522390687	24.920356173261276	26.1463553164454	25.108527987902633
23	22.508528716074764	27.61180172843791	26.60358673318924	23.276082822298086
24	21.807845083806544	29.09247907415341	26.180458044542288	22.91921779749776
25	22.999377412828697	27.029931515411153	26.134522519329934	23.836168552430216
26	21.45820109929719	27.36179596374188	27.20979003156626	23.970212905394664
27	20.220187115965054	33.16192970468979	24.775085833289843	21.84279734605531
28	20.967655807597506	27.30153971996927	27.57381541564672	24.156989056786504
29	22.322480736813105	26.008306016764948	26.653648567139292	25.015564679282654
30	23.572327517260714	24.82053591041541	26.503584427310823	25.103552145013055
31	23.443501731229237	26.9004989192712	24.420526686901763	25.2354726625978
32	20.623958562151312	32.11936925730083	23.797150662454595	23.459521518093258
33	21.458807909405678	26.757049009625224	25.032434000298547	26.75170908067055
34	20.446163200365056	26.473365283908244	26.434832842019418	26.645638673707282
35	20.440762590399537	26.313470820322316	26.332767381772154	26.912999207506
36	20.97123598723757	28.709460533677355	24.356204815402297	25.963098663682775
37	21.41972933841921	27.951615389189797	25.361931889206186	25.266723383184807
38	20.478202774093095	29.094663590543956	25.64312769347837	24.784005941884583
39	21.72186009143415	26.798918907110725	25.822075994470744	25.657145006984383
40	22.08479321731933	26.472515749756365	26.780835965877852	24.66185506704645
41	19.52927312644345	28.303201166046303	25.593672669636803	26.573853037873445
42	20.944354299431662	28.227835350572406	25.348339342776104	25.47947100721983
43	20.96310473178386	25.352404970502963	26.871129310020496	26.813360987692676
44	21.418455037191393	25.157376201635717	26.69309122419085	26.73107753698204
45	21.81421658994564	24.344493380308528	26.700737031557765	27.140552998188067
46	21.765247014190862	25.593551307615105	26.007517163623916	26.633684514570117
47	20.37225372915152	27.549664373328998	26.932356449966687	25.145725447552795
48	20.362969534491693	26.897586230750463	26.911118096169695	25.82832613858815
49	21.477861746812124	25.212231835442804	27.339647394782162	25.97025902296291
50	20.358964587775688	24.44655884055579	28.288152275355866	26.906324296312654
51	20.553871994621232	24.411181811231085	26.22700037986313	28.80794581428455
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	320.0
1	585.0
2	850.0
3	1923.5
4	2997.0
5	2341.0
6	1685.0
7	1652.5
8	1620.0
9	1723.0
10	1826.0
11	1934.5
12	2043.0
13	2174.0
14	2305.0
15	2295.0
16	2285.0
17	2420.5
18	2556.0
19	2724.5
20	2893.0
21	3238.0
22	3583.0
23	4260.5
24	4938.0
25	5988.5
26	8267.5
27	9496.0
28	11825.0
29	14154.0
30	17957.0
31	21760.0
32	24026.5
33	26293.0
34	31484.0
35	36675.0
36	40247.5
37	43820.0
38	49483.0
39	55146.0
40	60806.0
41	66466.0
42	75975.0
43	85484.0
44	96468.0
45	107452.0
46	124278.0
47	141104.0
48	154757.5
49	168411.0
50	171428.5
51	174446.0
52	159588.5
53	144731.0
54	129678.0
55	114625.0
56	103708.5
57	92792.0
58	85293.0
59	77794.0
60	70323.5
61	62853.0
62	56881.5
63	50910.0
64	44299.0
65	37688.0
66	31155.5
67	24623.0
68	21467.0
69	18311.0
70	15459.5
71	12608.0
72	10818.5
73	9029.0
74	7776.0
75	5082.0
76	3641.0
77	2829.0
78	2017.0
79	1614.0
80	1211.0
81	846.5
82	482.0
83	364.5
84	247.0
85	167.0
86	87.0
87	71.5
88	56.0
89	53.5
90	51.0
91	38.0
92	25.0
93	17.0
94	9.0
95	5.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1647962.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.28284297128019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.62229085765462	25.97603727516545
2	10.967498030046158	7.739290215638869
3	4.01162039043841	4.246241168886725
4	1.9979418913998606	2.819722800800153
5	1.1946861674102103	2.1075962222347506
6	0.8340859553838719	1.765735427301562
7	0.6029020270641104	1.489046828357867
8	0.49964126772161965	1.4103011512794619
9	0.40918675717708486	1.2993544889465798
>10	5.671071712051175	44.47056834668454
>50	0.1446556525453522	3.2109379990878693
>100	0.042003183937804625	2.653615399193417
>500	0.001486835181459119	0.31515208168131886
>1k	9.292719884119494E-4	0.4964005947414864
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	2455	0.148971881633193	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	1854	0.11250259411321377	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.068101084855112E-5	0.0	0.0	0.09108219728367523	0.0
2	6.068101084855112E-5	0.0	0.0	0.52476938181827	0.0
3	6.068101084855112E-5	0.0	0.0	0.7537188357498534	0.0
4	6.068101084855112E-5	0.0	0.0	1.0219289037004493	0.0
5	6.068101084855112E-5	0.0	0.0	1.820915773543322	0.0
6	6.068101084855112E-5	0.0	0.0	2.620570134505529	0.0
7	6.068101084855112E-5	0.0	0.0	3.247344295560213	0.0
8	6.068101084855112E-5	0.0	0.0	4.415028987318882	0.0
9	6.068101084855112E-5	0.0	0.0	4.904482020823296	0.0
10	6.068101084855112E-5	0.0	0.0	6.069678791137174	0.0
11	6.068101084855112E-5	0.0	0.0	7.4840924730060525	0.0
12	6.068101084855112E-5	0.0	0.0	8.639034152486525	0.0
13	6.068101084855112E-5	0.0	0.0	9.116472345842926	0.0
14	6.068101084855112E-5	0.0	0.0	9.314110398176657	0.0
15	6.068101084855112E-5	0.0	0.0	9.645428717409747	0.0
16	6.068101084855112E-5	0.0	0.0	10.387436118065828	0.0
17	6.068101084855112E-5	0.0	0.0	11.254021633993988	0.0
18	6.068101084855112E-5	0.0	0.0	12.331898429696802	0.0
19	6.068101084855112E-5	0.0	0.0	12.848293832017971	0.0
20	6.068101084855112E-5	0.0	0.0	13.378221099758369	6.068101084855112E-5
21	2.4272404339420448E-4	0.0	0.0	14.021318452731313	6.068101084855112E-5
22	5.461290976369601E-4	0.0	0.0	14.712839252361402	6.068101084855112E-5
23	5.461290976369601E-4	0.0	0.0	15.456120954245304	6.068101084855112E-5
24	6.068101084855112E-4	0.0	0.0	16.035260521783876	6.068101084855112E-5
25	0.001031577184425369	0.0	0.0	16.547893701432436	6.068101084855112E-5
26	0.0010922581952739201	0.0	0.0	17.03570834764394	6.068101084855112E-5
27	0.0011529392061224713	0.0	0.0	17.528377474723325	6.068101084855112E-5
28	0.0011529392061224713	0.0	0.0	18.078754243119683	6.068101084855112E-5
29	0.0011529392061224713	0.0	0.0	18.626036279962765	6.068101084855112E-5
30	0.0011529392061224713	0.0	0.0	19.29468033850295	6.068101084855112E-5
31	0.0011529392061224713	0.0	0.0	19.857800119177504	6.068101084855112E-5
32	0.0011529392061224713	0.0	0.0	20.441976210616506	6.068101084855112E-5
33	0.0011529392061224713	0.0	0.0	21.068568328638644	6.068101084855112E-5
34	0.0012743012278195736	0.0	0.0	21.667065138637906	6.068101084855112E-5
35	0.0013349822386681247	0.0	0.0	22.30852410431794	6.068101084855112E-5
36	0.0013349822386681247	0.0	0.0	22.924497045441584	6.068101084855112E-5
37	0.0013349822386681247	0.0	0.0	23.538467513207223	6.068101084855112E-5
38	0.001517025271213778	0.0	0.0	24.185569812896173	6.068101084855112E-5
39	0.001517025271213778	0.0	0.0	24.810280819582005	6.068101084855112E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAGT	30	2.166078E-6	45.000004	15
TCTAGCG	40	6.8175723E-9	45.0	1
CGACGGT	75	0.0	42.0	28
AACACGT	670	0.0	39.626865	41
CACGTGA	680	0.0	38.713234	43
ACACGTG	685	0.0	38.43066	42
ACACGAC	695	0.0	37.877697	26
AAACACG	730	0.0	37.602737	40
CCGTATA	30	1.1401368E-4	37.500004	34
CGTAAGG	390	0.0	37.5	2
CGTTAGG	325	0.0	37.384617	2
CGTATGG	200	0.0	37.125004	2
ACGTGAG	715	0.0	36.81818	44
CGTACTC	55	2.750312E-9	36.81818	45
CAATGCG	55	2.750312E-9	36.81818	1
GACACGA	730	0.0	36.36986	25
CGACCAA	745	0.0	36.24161	29
CACGACC	710	0.0	36.126762	27
CTCGTAG	25	0.002107373	36.000004	1
TCAAGCG	840	0.0	35.892857	17
>>END_MODULE