##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552546_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	795766
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61199397812925	31.0	31.0	34.0	30.0	34.0
2	31.74216666708555	31.0	31.0	34.0	30.0	34.0
3	31.73519225500964	31.0	31.0	34.0	30.0	34.0
4	35.44752603152183	37.0	35.0	37.0	33.0	37.0
5	35.35727085600541	37.0	35.0	37.0	33.0	37.0
6	35.35256092871523	37.0	35.0	37.0	33.0	37.0
7	35.756584221994906	37.0	35.0	37.0	35.0	37.0
8	35.69806953300342	37.0	35.0	37.0	35.0	37.0
9	37.29219142310679	39.0	37.0	39.0	35.0	39.0
10	36.473255957153235	39.0	35.0	39.0	32.0	39.0
11	36.55115197181081	38.0	35.0	39.0	32.0	39.0
12	36.482361649027474	38.0	35.0	39.0	32.0	39.0
13	36.08642364715256	38.0	35.0	39.0	32.0	39.0
14	36.0359263904213	39.0	35.0	40.0	30.0	41.0
15	36.778624620805616	39.0	35.0	41.0	31.0	41.0
16	37.21021883317458	39.0	35.0	41.0	32.0	41.0
17	37.36533352769533	39.0	36.0	41.0	32.0	41.0
18	37.38088332499755	39.0	36.0	41.0	32.0	41.0
19	37.38992618433057	39.0	36.0	41.0	32.0	41.0
20	37.18941372212434	39.0	35.0	40.0	32.0	41.0
21	37.16366997333387	39.0	35.0	40.0	32.0	41.0
22	37.180529200795206	39.0	35.0	40.0	32.0	41.0
23	36.813628629521745	39.0	35.0	40.0	31.0	41.0
24	35.23761382114843	39.0	35.0	40.0	27.0	41.0
25	36.046200767562325	39.0	35.0	40.0	28.0	41.0
26	36.43364506651453	38.0	35.0	40.0	30.0	41.0
27	32.70742529839174	38.0	33.0	40.0	6.0	41.0
28	34.239123058788635	38.0	33.0	40.0	16.0	41.0
29	35.46125996838266	38.0	34.0	40.0	26.0	41.0
30	36.00082059298839	38.0	34.0	40.0	30.0	41.0
31	36.22342623333995	38.0	35.0	40.0	30.0	41.0
32	33.38536705513933	38.0	33.0	40.0	15.0	41.0
33	34.07651998200476	38.0	33.0	40.0	16.0	41.0
34	35.07253639889113	38.0	33.0	40.0	25.0	41.0
35	35.34850697315542	38.0	34.0	40.0	27.0	41.0
36	35.48973064946228	38.0	34.0	40.0	28.0	41.0
37	35.66411105777326	38.0	34.0	40.0	29.0	41.0
38	35.620059163120814	38.0	34.0	40.0	29.0	41.0
39	35.73506533327637	38.0	34.0	40.0	29.0	41.0
40	35.56628581769012	38.0	34.0	40.0	28.0	41.0
41	35.35833021265045	38.0	34.0	40.0	27.0	41.0
42	35.28978116682542	38.0	34.0	40.0	27.0	41.0
43	35.32799717504895	38.0	34.0	40.0	28.0	41.0
44	35.279055652038416	37.0	34.0	40.0	27.0	41.0
45	35.226770181183916	37.0	34.0	40.0	27.0	41.0
46	34.997456538731235	37.0	34.0	40.0	26.0	41.0
47	34.82494100024379	37.0	33.0	40.0	26.0	41.0
48	34.68697581952483	37.0	33.0	40.0	26.0	41.0
49	34.67628800426256	37.0	33.0	40.0	26.0	41.0
50	34.538046360362216	36.0	33.0	40.0	26.0	41.0
51	33.00977297346205	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	20.0
9	54.0
10	88.0
11	63.0
12	40.0
13	36.0
14	25.0
15	43.0
16	103.0
17	206.0
18	380.0
19	714.0
20	1148.0
21	1761.0
22	2686.0
23	3790.0
24	5309.0
25	7163.0
26	9129.0
27	11458.0
28	14654.0
29	18759.0
30	23907.0
31	30131.0
32	38115.0
33	49887.0
34	71134.0
35	78458.0
36	75035.0
37	94879.0
38	129183.0
39	127390.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.701749006617526	19.235931165694435	24.606228464146497	13.456091363541544
2	31.31385357001933	23.857641567998634	25.7142174960981	19.114287365883943
3	32.312011319910624	24.78228524465735	26.166611792913997	16.739091642518027
4	29.47474508838025	26.888934686830048	24.03206470243765	19.604255522352048
5	23.801343611061544	30.002915429912814	25.40269375670737	20.793047202318267
6	24.918506194031913	35.22580758665236	24.199324927177084	15.656361292138643
7	81.44152929378737	5.9824119150604576	7.648730908332349	4.9273278828198235
8	81.61193114558802	4.350776484544451	7.934116310573712	6.103176059293813
9	76.35787404840116	6.731375806455667	10.402932520364025	6.507817624779144
10	39.52845434461889	24.631989806048512	18.151315839078322	17.688240010254273
11	30.357039632253702	26.62516367876989	22.426567609071007	20.5912290799054
12	27.757406071634122	21.5672697752857	29.77470764018568	20.900616512894494
13	25.03474639529711	25.030850777741193	29.75296758092203	20.181435246039666
14	19.689330783169925	30.88583829919851	27.97505799443555	21.449772923196015
15	18.786050170527517	25.65251594061571	34.36914871959848	21.192285169258298
16	21.43042050049889	23.808883516008475	31.854213424549428	22.906482558943207
17	20.592108735482544	23.587210310568686	27.6889186016995	28.13176235224928
18	23.1003837811618	23.94334515422876	29.788405134172606	23.167865930436836
19	23.736123433270585	26.6956617900237	26.641877134735587	22.926337641970125
20	24.391341173158942	25.942048290577883	26.305848704267337	23.360761831995838
21	23.445208767401475	27.82715019239324	27.761553019354935	20.96608802085035
22	20.87108521851901	25.45798639298487	26.0115410811721	27.659387307324014
23	20.111691125280547	27.241425243099105	26.859780387701914	25.78710324391844
24	20.73762890095832	28.558269642080713	27.014599769278906	23.689501687682057
25	21.468371355398446	26.247665771093516	26.55743019932995	25.726532674178088
26	20.15642789463234	27.950301973193127	27.338941347079416	24.554328785095116
27	19.861366281042418	33.06122654147073	25.141687380461093	21.93571979702576
28	18.768834054232023	26.309115996411002	29.156812429784633	25.765237519572338
29	22.60589168172553	24.197942611270147	28.010621212768577	25.185544494235746
30	24.929439056204966	23.92625470301571	27.76657962265289	23.377726618126434
31	25.076592867752577	26.011918076419448	24.430800009047886	24.480689046780082
32	23.021591774466362	30.473028503353998	24.33139892883084	22.1739807933488
33	24.365579831256927	25.48362206980444	25.69926335128668	24.451534747651948
34	23.668389953830648	23.49484647496877	27.383929446596113	25.45283412460447
35	23.367547746448075	22.785341419462505	29.74530201089265	24.10180882319677
36	25.861496972728165	25.670737377570795	26.857015755888035	21.610749893813004
37	23.59550420601031	24.63902705066565	28.508003609101166	23.257465134222873
38	22.05937423815544	26.510431458494082	28.155136057584766	23.275058245765713
39	25.882608706579575	23.632324075167826	26.458154784195354	24.026912434057248
40	28.07873168745586	21.67534174619172	28.62650578185044	21.619420784501976
41	22.57849669375168	23.610207020656826	27.490493436512743	26.32080284907875
42	23.483662282630824	24.765069128361855	27.62897635737139	24.12229223163593
43	22.294493607417255	22.582140981142697	30.149189585883285	24.97417582555676
44	22.991934815008435	22.58741891460555	27.86598070286994	26.55466556751608
45	24.8212414202165	21.106455917945727	28.27765951297241	25.79464314886537
46	26.609581208546228	22.45170062556078	27.443871690924215	23.49484647496877
47	23.1168459069626	23.24804025303921	30.26015185368563	23.37496198631256
48	21.75802937044307	22.514784496950107	30.570795937499213	25.15639019510761
49	23.777718575561156	21.55633691311265	30.420751829055277	24.24519268227092
50	22.441396088799973	20.44910187165574	30.845374142649973	26.264127896894312
51	21.86057207772134	21.009945134625003	27.693316879585204	29.43616590806845
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	504.0
1	689.5
2	875.0
3	1255.5
4	1636.0
5	1257.5
6	879.0
7	887.5
8	896.0
9	1000.0
10	1104.0
11	1169.5
12	1235.0
13	1322.0
14	1409.0
15	1414.5
16	1420.0
17	1402.0
18	1384.0
19	1483.5
20	1583.0
21	1753.0
22	1923.0
23	2114.5
24	2306.0
25	2666.5
26	3401.5
27	3776.0
28	5223.5
29	6671.0
30	7453.5
31	8236.0
32	9447.5
33	10659.0
34	11898.0
35	13137.0
36	14835.0
37	16533.0
38	18833.0
39	21133.0
40	24847.5
41	28562.0
42	33426.0
43	38290.0
44	48279.0
45	58268.0
46	67062.0
47	75856.0
48	85446.5
49	95037.0
50	94614.5
51	94192.0
52	82478.5
53	70765.0
54	61674.5
55	52584.0
56	46805.5
57	41027.0
58	37153.0
59	33279.0
60	30895.5
61	28512.0
62	25559.0
63	22606.0
64	19787.0
65	16968.0
66	14619.0
67	12270.0
68	10446.5
69	8623.0
70	7519.5
71	6416.0
72	5775.0
73	5134.0
74	4187.5
75	2433.0
76	1625.0
77	1292.5
78	960.0
79	777.0
80	594.0
81	465.5
82	337.0
83	259.5
84	182.0
85	125.5
86	69.0
87	45.5
88	22.0
89	14.5
90	7.0
91	7.0
92	7.0
93	5.0
94	3.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	795766.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.9171139266124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.80018909871563	28.74124405714764
2	10.565309044380774	8.012120534113084
3	4.147273488624377	4.717579240589693
4	1.9923575213718032	3.0217778848159105
5	1.1474527578952545	2.175404847325998
6	0.7243843555850843	1.6479938482425214
7	0.5402579637130162	1.4339515931906244
8	0.41585684191426203	1.2614473001619453
9	0.3112871601919488	1.0622799645200791
>10	4.023296384474443	36.63361904090743
>50	0.2858573568675815	6.591182142830167
>100	0.042633978980608105	2.96439532838918
>500	0.0020967530646200587	0.47807698662739706
>1k	0.0017472942205167157	1.2589272311382758
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	3087	0.38792810951963264	TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	2467	0.31001575840133905	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGCT	1454	0.1827170298806433	Illumina Paired End PCR Primer 2 (95% over 21bp)
TGAATGATACCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCC	1449	0.182088704468399	No Hit
TCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	1044	0.13119434607661046	TruSeq Adapter, Index 21 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24705755209445993	0.0
2	0.0	0.0	0.0	1.4804603363300266	0.0
3	0.0	0.0	0.0	2.073222530241302	0.0
4	0.0	0.0	0.0	2.703307253639889	0.0
5	0.0	0.0	0.0	4.406948776399092	0.0
6	0.0	0.0	0.0	5.698283163643584	0.0
7	0.0	0.0	0.0	6.825247623044966	0.0
8	0.0	0.0	0.0	8.816033859199814	0.0
9	0.0	0.0	0.0	9.622049698026807	0.0
10	0.0	0.0	0.0	11.28485006899013	0.0
11	0.0	0.0	0.0	13.705033891872736	0.0
12	0.0	0.0	0.0	15.447003264778843	0.0
13	0.0	0.0	0.0	16.27111487547847	0.0
14	0.0	0.0	0.0	16.525461002354962	0.0
15	0.0	0.0	0.0	17.044583457951205	0.0
16	0.0	0.0	0.0	18.177202846062787	0.0
17	0.0	0.0	0.0	19.443655546982406	0.0
18	0.0	0.0	0.0	21.1588582573269	0.0
19	0.0	0.0	0.0	21.841219655024215	0.0
20	0.0	0.0	0.0	22.563668214022716	0.0
21	1.256650824488606E-4	0.0	0.0	23.325575608910157	0.0
22	7.539904946931636E-4	0.0	0.0	24.089116649869435	0.0
23	0.0016336460718351876	0.0	0.0	24.831294626812404	0.0
24	0.0017593111542840484	0.0	0.0	25.43184805583551	0.0
25	0.001884976236732909	0.0	0.0	25.950970511431752	0.0
26	0.0020106413191817696	0.0	0.0	26.43327309787048	0.0
27	0.00213630640163063	0.0	0.0	26.941211361128776	0.0
28	0.0022619714840794907	0.0	0.0	27.492504077831924	0.0
29	0.0022619714840794907	0.0	0.0	28.015396485901633	0.0
30	0.0022619714840794907	0.0	0.0	28.624997800861056	0.0
31	0.0022619714840794907	0.0	0.0	29.143994591374852	0.0
32	0.0026389667314260725	0.0	0.0	29.65042487364376	0.0
33	0.002764631813874933	0.0	0.0	30.18425014388652	0.0
34	0.0030159619787726542	0.0	0.0	30.688795449918693	0.0
35	0.0030159619787726542	0.0	0.0	31.291359520260983	0.0
36	0.0030159619787726542	0.0	0.0	31.83712297333638	0.0
37	0.003392957226119236	0.0	0.0	32.36076937190079	0.0
38	0.0035186223085680968	0.0	0.0	32.91595770615985	0.0
39	0.0035186223085680968	0.0	0.0	33.417612715295704	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.031976E-4	45.000004	1
CGAACGC	20	7.031976E-4	45.000004	16
ACCGAAC	40	6.8102963E-9	45.000004	10
GCACGAT	20	7.031976E-4	45.000004	41
TGCGACA	20	7.031976E-4	45.000004	30
AGTTCGC	20	7.031976E-4	45.000004	1
CGTACGC	20	7.031976E-4	45.000004	13
GACGATT	20	7.031976E-4	45.000004	10
CCCGAAT	20	7.031976E-4	45.000004	22
CGTGATA	25	3.8897197E-5	45.0	6
CCGTAGT	45	3.8380676E-10	45.0	27
ACCGTAG	45	3.8380676E-10	45.0	26
CGCGTAC	25	3.8897197E-5	45.0	43
TATGCAG	95	0.0	42.63158	1
ATCACGC	60	3.6379788E-12	41.250004	42
GATCCTC	55	6.002665E-11	40.909092	9
CACCGTA	55	6.002665E-11	40.909092	25
GCTACGA	105	0.0	40.714287	10
CGTTAGG	50	1.0804797E-9	40.5	2
CTACGCG	50	1.0804797E-9	40.5	1
>>END_MODULE