##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552539_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1252232
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.55719227746935	31.0	31.0	34.0	30.0	34.0
2	31.6698654881843	31.0	31.0	34.0	30.0	34.0
3	31.641186297746742	31.0	31.0	34.0	30.0	34.0
4	35.378547266001824	37.0	35.0	37.0	33.0	37.0
5	35.310909639747265	37.0	35.0	37.0	33.0	37.0
6	35.32187565882361	37.0	35.0	37.0	33.0	37.0
7	35.79745606245488	37.0	35.0	37.0	35.0	37.0
8	35.75673277795169	37.0	35.0	37.0	35.0	37.0
9	37.3832045499556	39.0	37.0	39.0	35.0	39.0
10	36.40286224916789	38.0	35.0	39.0	32.0	39.0
11	36.5240498565761	38.0	35.0	39.0	32.0	39.0
12	36.51687706431396	38.0	35.0	39.0	32.0	39.0
13	36.1723801979186	38.0	35.0	39.0	32.0	39.0
14	36.10065467101943	39.0	36.0	40.0	30.0	41.0
15	36.81540880603594	39.0	36.0	40.0	31.0	41.0
16	37.24815689105533	39.0	36.0	41.0	32.0	41.0
17	37.451895495403406	39.0	36.0	41.0	32.0	41.0
18	37.46170118636163	39.0	36.0	40.0	32.0	41.0
19	37.44845923119677	39.0	36.0	40.0	32.0	41.0
20	37.24408576046611	39.0	36.0	40.0	32.0	41.0
21	37.23956742839985	39.0	35.0	40.0	32.0	41.0
22	37.25196529077679	39.0	35.0	40.0	32.0	41.0
23	36.87925959406883	39.0	35.0	40.0	31.0	41.0
24	35.2635901334577	39.0	35.0	40.0	27.0	41.0
25	36.13330676743607	39.0	35.0	40.0	28.0	41.0
26	36.571591366456055	39.0	35.0	40.0	30.0	41.0
27	32.71911434941768	38.0	33.0	40.0	0.0	41.0
28	34.32281318477726	38.0	33.0	40.0	16.0	41.0
29	35.614742316120335	38.0	34.0	40.0	26.0	41.0
30	36.231666336589385	38.0	35.0	40.0	30.0	41.0
31	36.46839243846188	38.0	35.0	40.0	30.0	41.0
32	33.62268892665257	38.0	34.0	40.0	15.0	41.0
33	34.38027138741064	38.0	34.0	40.0	16.0	41.0
34	35.36869845204403	38.0	34.0	40.0	25.0	41.0
35	35.83631787080988	38.0	35.0	40.0	29.0	41.0
36	36.01277319218803	38.0	35.0	40.0	30.0	41.0
37	36.14797817017933	38.0	35.0	40.0	30.0	41.0
38	36.18121082994205	38.0	35.0	40.0	30.0	41.0
39	36.236738879057555	38.0	35.0	40.0	30.0	41.0
40	36.16515629691623	38.0	35.0	40.0	30.0	41.0
41	36.08883337911825	38.0	35.0	40.0	30.0	41.0
42	36.000444007180775	38.0	35.0	40.0	29.0	41.0
43	36.016233413616646	38.0	35.0	40.0	30.0	41.0
44	35.93223380332079	38.0	35.0	40.0	29.0	41.0
45	35.88365893859924	38.0	35.0	40.0	29.0	41.0
46	35.73114486772419	38.0	35.0	40.0	29.0	41.0
47	35.638775402641045	38.0	34.0	40.0	29.0	41.0
48	35.60144366219678	38.0	34.0	40.0	29.0	41.0
49	35.587948559052954	38.0	34.0	40.0	28.0	41.0
50	35.44262884193983	38.0	34.0	40.0	28.0	41.0
51	34.10396875339394	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	26.0
9	71.0
10	112.0
11	73.0
12	44.0
13	24.0
14	34.0
15	54.0
16	87.0
17	200.0
18	405.0
19	756.0
20	1418.0
21	2340.0
22	3687.0
23	5289.0
24	7503.0
25	10450.0
26	13218.0
27	16312.0
28	20298.0
29	25773.0
30	33789.0
31	43445.0
32	56445.0
33	74587.0
34	104536.0
35	115979.0
36	118335.0
37	150447.0
38	213523.0
39	232946.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.82473535255448	20.18787253480186	23.139322425876355	10.848069686767309
2	33.51559455436372	23.075755930211013	24.631617783286163	18.777031732139093
3	32.7828229912668	23.839512167074474	26.238588376594752	17.139076465063983
4	29.025212580416408	26.016824358425595	24.98602495384242	19.971938107315577
5	23.271087146790688	30.84867660305758	26.12095841665123	19.7592778335005
6	22.80088673664305	35.820199451858755	26.017862504711587	15.361051306786603
7	80.96287269451668	5.432859086814584	8.525097585750883	5.079170632917862
8	81.37661391818767	4.1793373751828735	8.299899699097292	6.1441490075321505
9	76.18716020673484	6.28270160800874	11.245200569862455	6.284937615393953
10	36.70925195970076	23.386560956755616	22.313117697040166	17.59106938650346
11	29.430089631953184	27.26052360904369	22.598847497907737	20.710539261095388
12	24.597358955848435	23.21814168620511	30.500498310217278	21.684001047729176
13	24.50105092347105	25.242127656855917	31.51380894275182	18.74301247692121
14	21.202940030281926	30.10097170492369	28.330133713241633	20.365954551552747
15	19.93680084840509	26.52998805333197	33.552568533626356	19.980642564636586
16	21.243906879875293	25.438656734534813	31.312009276236353	22.005427109353537
17	21.491464840381017	24.889397491838572	27.918947926582295	25.700189741198116
18	23.10322687808649	24.453136479502202	31.210430655022392	21.233205987388917
19	22.34681752263159	27.029496131707226	28.22248592912495	22.40120041653623
20	23.567837269771097	26.334337407125837	28.893208287282228	21.20461703582084
21	22.475547662094563	28.250276306626887	29.065141283723783	20.209034747554767
22	21.043544646678892	26.000453590069572	27.0845977422714	25.871404020980137
23	18.963658491397762	27.953925470679554	28.8313187971558	24.251097240766885
24	19.39297190935865	28.544950137035308	28.281819982239714	23.78025797136633
25	19.47115231043449	27.916073059944164	27.62195823138204	24.990816398239303
26	19.254658881101903	27.035964581643018	30.921506557890233	22.787869979364846
27	19.56346747248114	33.88046304518652	26.13197873876406	20.424090743568286
28	18.14472078656351	27.702294782436482	30.73495965603818	23.41802477496183
29	19.63246427179628	25.094072024992176	31.641341221115578	23.63212248209597
30	22.625040727277373	25.68653412466699	29.969765985855656	21.718659162199977
31	20.990279756466855	29.61511924307956	26.765487545438866	22.629113455014725
32	18.97811268199503	32.449338461243606	26.911546742137237	21.66100211462413
33	20.45715170990679	26.60585258961598	27.76969443361933	25.1673012668579
34	18.37606769352644	26.96680806751465	30.865286943633446	23.791837295325465
35	19.257613605146652	24.347804560177348	30.293827341898304	26.100754492777696
36	21.26083665007762	26.520644736758044	28.765037149665556	23.453481463498775
37	19.95468890748679	27.722578563716628	29.034555896990334	23.288176631806245
38	18.256042011384473	28.147260252093858	28.65746922295549	24.939228513566174
39	20.082540615477004	24.266190290617075	30.45434072919395	25.19692836471197
40	21.5525557564413	24.349082278683184	30.596646627781432	23.501715337094083
41	19.276699525327576	24.807224220431998	27.168128589590427	28.747947664650003
42	21.922535121287428	23.672929616876107	28.286371854416753	26.118163407419708
43	22.368139450197727	22.98216304965853	29.047812226488386	25.601885273655363
44	20.500274709478756	24.834934740527316	29.05851311897476	25.60627743101917
45	20.669971698535093	23.106580889164306	30.39947869084962	25.823968721450978
46	22.31774942662382	24.254770681471165	29.13693309227044	24.290546799634573
47	18.41743383015288	25.549898101949157	30.868081952864962	25.164586115033
48	19.2362118201739	23.20983651591718	30.14768828779332	27.406263376115607
49	21.352113665838278	21.275131125861662	31.44393371196392	25.92882149633614
50	19.806233988589973	21.872704099559826	31.85663678934894	26.46442512250126
51	19.661931654837122	21.36289441573127	27.892515124992812	31.082658804438797
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	718.0
1	835.0
2	952.0
3	2183.0
4	3414.0
5	2628.0
6	1842.0
7	1859.0
8	1876.0
9	1952.0
10	2028.0
11	2209.5
12	2391.0
13	2569.0
14	2747.0
15	2684.5
16	2622.0
17	2790.0
18	2958.0
19	2992.5
20	3027.0
21	3156.0
22	3285.0
23	3514.0
24	3743.0
25	4435.0
26	5778.5
27	6430.0
28	7562.5
29	8695.0
30	10740.5
31	12786.0
32	14813.0
33	16840.0
34	19717.0
35	22594.0
36	26780.0
37	30966.0
38	35547.0
39	40128.0
40	50281.5
41	60435.0
42	75064.0
43	89693.0
44	110575.5
45	131458.0
46	148334.0
47	165210.0
48	163853.0
49	162496.0
50	141865.5
51	121235.0
52	106148.5
53	91062.0
54	78309.0
55	65556.0
56	58761.5
57	51967.0
58	46241.5
59	40516.0
60	35686.0
61	30856.0
62	26662.5
63	22469.0
64	19039.0
65	15609.0
66	13033.5
67	10458.0
68	8136.0
69	5814.0
70	5202.0
71	4590.0
72	3649.5
73	2709.0
74	2564.0
75	1778.0
76	1137.0
77	910.0
78	683.0
79	519.0
80	355.0
81	267.5
82	180.0
83	123.0
84	66.0
85	59.0
86	52.0
87	37.0
88	22.0
89	15.0
90	8.0
91	6.5
92	5.0
93	3.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1252232.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.15136872892381
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.50938923970503	26.938230559533427
2	11.893020325343123	8.836839668148158
3	5.018844890785271	5.593708713925171
4	2.599461098063964	3.8629415100267
5	1.5529031823077621	2.884623936311744
6	0.9977669386126887	2.2241044465157693
7	0.6621143120224394	1.7218917062650354
8	0.5043619217791941	1.499018858307798
9	0.3823256383238904	1.2783528687502816
>10	3.4205691380979126	29.62243264720199
>50	0.40184698727961793	9.381614620437094
>100	0.0527425715073505	3.739766883029863
>500	0.0026592892409913944	0.6643223511806052
>1k	0.0017728594939942631	1.2549591769792992
>5k	2.216074367492829E-4	0.4971920533869965
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	6039	0.48225887854646743	Illumina PCR Primer Index 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	4485	0.35816046866714796	RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	3405	0.27191446952321935	TruSeq Adapter, Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1670	0.13336186904663033	Illumina PCR Primer Index 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1387	0.11076222297465646	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.98574066147487E-5	0.0	0.0	0.32997080413214164	0.0
2	7.98574066147487E-5	0.0	0.0	1.7046362015984258	0.0
3	7.98574066147487E-5	0.0	0.0	2.3482070415066856	0.0
4	7.98574066147487E-5	0.0	0.0	3.0836138990219064	0.0
5	7.98574066147487E-5	0.0	0.0	4.989490765289499	0.0
6	7.98574066147487E-5	0.0	0.0	6.164113359185838	0.0
7	7.98574066147487E-5	0.0	0.0	7.233723463383782	0.0
8	7.98574066147487E-5	0.0	0.0	9.177293025573537	0.0
9	7.98574066147487E-5	0.0	0.0	9.853126257754154	0.0
10	7.98574066147487E-5	0.0	0.0	11.717876559615151	0.0
11	7.98574066147487E-5	0.0	0.0	14.170936376007003	0.0
12	7.98574066147487E-5	0.0	0.0	16.050220725871885	0.0
13	7.98574066147487E-5	0.0	0.0	16.926575906062137	0.0
14	7.98574066147487E-5	0.0	0.0	17.21741658095305	0.0
15	7.98574066147487E-5	0.0	0.0	17.82026014348779	0.0
16	7.98574066147487E-5	0.0	0.0	19.144375802566937	0.0
17	7.98574066147487E-5	0.0	0.0	20.64329932472577	0.0
18	7.98574066147487E-5	0.0	0.0	22.545263178069238	0.0
19	7.98574066147487E-5	0.0	0.0	23.41147646761942	0.0
20	7.98574066147487E-5	0.0	0.0	24.25908298142836	0.0
21	4.7914443968849225E-4	0.0	0.0	25.274070619501817	0.0
22	5.59001846303241E-4	0.0	0.0	26.31876521283596	0.0
23	0.001038146285991733	0.0	0.0	27.423033431504706	0.0
24	0.004232442550581681	0.0	0.0	28.247161867768913	0.0
25	0.005510161056417661	0.0	0.0	29.012355537951432	0.0
26	0.005669875869647158	0.0	0.0	29.691622638616487	0.0
27	0.005829590682876655	0.0	0.0	30.368014872643407	0.0
28	0.005909448089491404	0.0	0.0	31.074273776744246	0.0
29	0.005909448089491404	0.0	0.0	31.82333625079059	0.0
30	0.006628164749024143	0.0	0.0	32.65608928696919	0.0
31	0.00678787956225364	0.0	0.0	33.38183339828402	0.0
32	0.00678787956225364	0.0	0.0	34.108296226258396	0.0
33	0.006947594375483137	0.0	0.0	34.883631787080986	0.0
34	0.006947594375483137	0.0	0.0	35.65186003871487	0.0
35	0.007267024001942132	0.0	0.0	36.4506736770822	0.0
36	0.007267024001942132	0.0	0.0	37.200454867788075	0.0
37	0.007586453628401127	0.0	0.0	37.92787598464182	0.0
38	0.007746168441630625	0.0	0.0	38.65960940145277	0.0
39	0.00806559806808962	0.0	0.0	39.40723444218004	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	40	6.8157533E-9	45.0	1
GAACGTA	20	7.0335285E-4	45.0	9
GCCGTAA	20	7.0335285E-4	45.0	41
CGCTTCG	25	3.8910075E-5	45.0	2
TAACGCA	165	0.0	43.636368	26
TATTGCG	90	0.0	42.5	1
TCGATTG	55	6.184564E-11	40.909092	1
CGTTTTT	2140	0.0	40.68925	1
CTACGCA	155	0.0	40.64516	15
GTCGATC	50	1.0822987E-9	40.5	6
GCGAGAC	340	0.0	40.367645	21
TCGTTGC	95	0.0	40.26316	16
CGACTAA	135	0.0	40.0	17
TCAAGCG	415	0.0	39.578312	17
ACGCATT	165	0.0	39.545456	17
GTACGAT	330	0.0	39.545456	10
GACACGA	325	0.0	39.46154	25
ACGCATA	40	3.4585537E-7	39.375	21
TTGTACG	40	3.4585537E-7	39.375	1
CGACAAT	40	3.4585537E-7	39.375	20
>>END_MODULE