SRR3552536_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552536_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	732798
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	10857	1.4815815545348106	TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	8565	1.1688077751303907	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCG	8507	1.1608929063671025	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC	7702	1.0510399864628452	No Hit
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT	7369	1.005597722701208	TruSeq Adapter, Index 22 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGC	6867	0.9370931689224044	No Hit
TCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	3466	0.4729816402337343	TruSeq Adapter, Index 20 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	2419	0.33010461273092995	TruSeq Adapter, Index 22 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	1791	0.24440568888015524	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCT	1779	0.24276812982568183	No Hit
AGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	1673	0.22830302484450013	TruSeq Adapter, Index 22 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	1408	0.19214026239154583	No Hit
CGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	1381	0.18845575451898067	TruSeq Adapter, Index 22 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	1272	0.17358125977418062	TruSeq Adapter, Index 22 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTC	1252	0.1708519946833916	No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCT	1151	0.15706920597490714	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1083	0.14778970466622451	No Hit
AAGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC	994	0.13564447501221347	No Hit
TGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	965	0.1316870406305694	TruSeq Adapter, Index 22 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTA	956	0.13045887133971437	No Hit
GGCCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCT	909	0.1240450983763602	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTGTTTT	814	0.11108108919511243	No Hit
GGGCCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC	801	0.10930706688609956	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCC	782	0.10671426504985002	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	780	0.10644133854077113	No Hit
AGGCCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC	768	0.10480377948629772	No Hit
GGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCT	760	0.10371207344998214	No Hit
AGCCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCT	748	0.10207451439550871	No Hit
GTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	738	0.10070988185011422	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	20	7.0316094E-4	45.000004	1
CGGGTAT	40	6.8084773E-9	45.000004	6
TCGTTCG	20	7.0316094E-4	45.000004	45
TCGTTAG	20	7.0316094E-4	45.000004	1
TCGCACA	20	7.0316094E-4	45.000004	16
TCGCAAA	20	7.0316094E-4	45.000004	10
TTAATCG	45	3.8380676E-10	45.000004	20
GATCGGC	20	7.0316094E-4	45.000004	10
GGTAACT	45	3.8380676E-10	45.000004	8
GATCGAT	20	7.0316094E-4	45.000004	12
ACGTTAG	20	7.0316094E-4	45.000004	1
TAGACCG	20	7.0316094E-4	45.000004	2
AACCCGA	20	7.0316094E-4	45.000004	36
CCCTACA	20	7.0316094E-4	45.000004	27
TTTCGCG	20	7.0316094E-4	45.000004	1
GACAATT	20	7.0316094E-4	45.000004	35
CGCATAG	20	7.0316094E-4	45.000004	27
CGTTGAT	45	3.8380676E-10	45.000004	25
CTCCCGT	20	7.0316094E-4	45.000004	13
GAATCGT	20	7.0316094E-4	45.000004	18

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content