##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552536_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	732798
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.204184509237194	33.0	31.0	34.0	30.0	34.0
2	32.42198259274725	34.0	31.0	34.0	30.0	34.0
3	32.223019167628735	34.0	31.0	34.0	30.0	34.0
4	35.857327667379	37.0	35.0	37.0	35.0	37.0
5	35.88581164249902	37.0	35.0	37.0	35.0	37.0
6	35.91492334858992	37.0	35.0	37.0	35.0	37.0
7	36.13038654581481	37.0	35.0	37.0	35.0	37.0
8	36.04182052898616	37.0	35.0	37.0	35.0	37.0
9	37.8566330694134	39.0	38.0	39.0	35.0	39.0
10	37.45221466215792	39.0	37.0	39.0	35.0	39.0
11	37.24945073540048	39.0	37.0	39.0	34.0	39.0
12	37.15299850709199	39.0	37.0	39.0	34.0	39.0
13	37.02295857794372	39.0	37.0	39.0	33.0	39.0
14	38.12803664857164	40.0	37.0	41.0	33.0	41.0
15	38.10953223125609	40.0	37.0	41.0	33.0	41.0
16	38.23726183750502	40.0	37.0	41.0	34.0	41.0
17	38.15881730026556	40.0	37.0	41.0	34.0	41.0
18	38.150749592657185	40.0	37.0	41.0	34.0	41.0
19	38.17721254697747	40.0	37.0	41.0	34.0	41.0
20	38.12177980835101	40.0	37.0	41.0	34.0	41.0
21	38.07300647654606	40.0	37.0	41.0	34.0	41.0
22	37.99997134271655	40.0	37.0	41.0	34.0	41.0
23	37.98621721129152	40.0	37.0	41.0	34.0	41.0
24	37.87337984001048	40.0	36.0	41.0	33.0	41.0
25	37.77022044274138	40.0	36.0	41.0	33.0	41.0
26	37.70871918318554	39.0	36.0	41.0	33.0	41.0
27	37.702286305366556	40.0	36.0	41.0	33.0	41.0
28	37.6971020663266	40.0	36.0	41.0	33.0	41.0
29	37.64389367874912	40.0	36.0	41.0	33.0	41.0
30	37.52630192767993	39.0	36.0	41.0	33.0	41.0
31	37.34618271338077	39.0	36.0	41.0	32.0	41.0
32	37.24505116007413	39.0	36.0	41.0	32.0	41.0
33	37.278207637029574	39.0	36.0	41.0	32.0	41.0
34	37.147170161490614	39.0	35.0	41.0	31.0	41.0
35	37.154716579466644	39.0	35.0	41.0	32.0	41.0
36	37.137934328423384	39.0	35.0	41.0	32.0	41.0
37	37.04172909860562	39.0	35.0	41.0	31.0	41.0
38	37.056147806080254	39.0	35.0	41.0	31.0	41.0
39	36.97774693708225	39.0	35.0	41.0	31.0	41.0
40	36.79973062153554	39.0	35.0	40.0	31.0	41.0
41	36.66755777171881	39.0	35.0	40.0	31.0	41.0
42	36.78738069699972	39.0	35.0	40.0	31.0	41.0
43	36.772186878239296	39.0	35.0	40.0	31.0	41.0
44	36.62865619174725	39.0	35.0	40.0	31.0	41.0
45	36.540438156217675	39.0	35.0	40.0	31.0	41.0
46	36.41187885338115	39.0	35.0	40.0	30.0	41.0
47	36.314665160112334	39.0	35.0	40.0	30.0	41.0
48	36.368236539946885	39.0	35.0	40.0	30.0	41.0
49	36.3225131618809	39.0	35.0	40.0	30.0	41.0
50	36.17180860209771	39.0	35.0	40.0	30.0	41.0
51	33.51820283352302	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	4.0
12	12.0
13	11.0
14	25.0
15	28.0
16	72.0
17	113.0
18	228.0
19	400.0
20	588.0
21	922.0
22	1398.0
23	2056.0
24	2707.0
25	3629.0
26	4477.0
27	5656.0
28	6845.0
29	8264.0
30	10908.0
31	14032.0
32	18621.0
33	26712.0
34	46534.0
35	54879.0
36	55527.0
37	89879.0
38	171573.0
39	206670.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.99562225879437	20.5849360942579	22.563789748334464	10.85565189861326
2	30.122762343783688	27.571854726677746	23.126973599818776	19.178409329719788
3	31.79539245467373	26.955859595686672	24.961722057101685	16.287025892537915
4	30.899647651876776	22.885843029047567	27.67529387361865	18.539215445457003
5	26.695897095789018	27.634491360511355	24.117423901266104	21.55218764243352
6	24.82403063327138	34.054132243810706	25.465407929606794	15.656429193311116
7	71.55218764243352	5.6700482261141545	15.42744385219392	7.350320279258405
8	65.34133553857953	8.91992063297116	14.908474095180392	10.830269733268922
9	59.45185439916594	8.564024465132274	17.32646650236491	14.65765463333688
10	36.640929696860525	22.855821113048886	24.620563920753057	15.882685269337527
11	27.682389962854703	23.58125977418061	27.16669532395012	21.56965493901457
12	27.622346130857345	19.359632531748176	33.1339605184512	19.884060818943283
13	22.001424676377393	24.331125357874885	34.37127284736039	19.29617711838733
14	16.446414973839993	29.43990021806828	29.629993531641734	24.48369127644999
15	15.479163425664371	27.361837777941535	37.84207926331677	19.31691953307733
16	16.7930316403702	28.648003951975852	30.121261247983753	24.437703159670196
17	15.771604180142413	29.126171195882083	29.27641723913002	25.825807384845483
18	16.53661718509057	29.57718225213497	32.13818815007683	21.74801241269763
19	19.40807698710968	28.73233824328123	27.11142770586164	24.748157063747446
20	21.225085221302457	29.023823754977496	31.170936601901207	18.580154421818836
21	20.784445372394575	29.865529108976823	29.954776077445626	19.395249441182973
22	16.660116430448774	27.61525004162129	26.6657387165358	29.058894811394136
23	19.451881691816844	27.096689674371383	29.279965283748044	24.17146335006373
24	24.01248365852527	23.015892510623665	27.112519411897956	25.85910441895311
25	18.62955411996212	29.54129241619109	24.540050600574784	27.289102863272007
26	20.37328158646721	27.249801445964643	27.167650566731893	25.209266400836245
27	23.304102904210982	25.771904399302397	24.667097890551013	26.256894805935605
28	16.321824022445476	25.05055963580687	27.967461701587617	30.66015464016004
29	24.750340475820074	21.508519400980898	27.70818151796266	26.032958605236367
30	23.91272901945693	23.70666950510236	25.296739346996034	27.083862128444675
31	21.10335999825327	23.82621131607892	25.9407094451677	29.129719240500112
32	22.96772098177124	23.764802851536167	30.438401851533435	22.829074315159158
33	23.293322307102365	21.043043239746833	29.35078971285402	26.312844740296782
34	20.526393358060474	20.024754434373456	34.67940687611047	24.7694453314556
35	20.93237154031534	18.735313142230194	32.72429782832377	27.608017489130702
36	22.804510929342054	21.28744892862699	31.054260519269977	24.853779622760978
37	17.62627627258808	21.36386835116908	37.316695733339884	23.693159642902955
38	19.357722046184627	22.54836940057151	31.756091037366367	26.3378175158775
39	25.425697122535816	18.63692313570725	36.22266982169711	19.714709920059825
40	24.576486289536817	18.009874481098475	36.98413478202725	20.429504447337465
41	18.695738798413753	22.584532163024463	32.870995826953674	25.84873321160811
42	19.990775083993135	18.879281875769312	35.62864527468689	25.501297765550667
43	24.953670725083857	18.409029500626367	31.446455912816358	25.19084386147342
44	21.317607307880206	19.81514687540086	29.47947456188472	29.38777125483421
45	21.444108744838278	17.272836443330906	29.45682166163117	31.826233150199645
46	24.64772010840641	20.01097164566497	31.813405604272937	23.527902641655682
47	17.43004211256035	18.158892355055553	39.61255352771159	24.798512004672503
48	18.838888752425635	17.106624199301855	34.21488595765818	29.83960109061433
49	20.728904827797017	15.414070453249055	39.08430426938938	24.772720449564545
50	20.293177656052556	15.254817835201514	35.35012377217186	29.101880736574063
51	18.923495970240094	15.430309580539248	30.795116798899556	34.8510776503211
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	344.0
1	683.5
2	1023.0
3	1070.0
4	1117.0
5	952.5
6	788.0
7	791.0
8	794.0
9	861.0
10	928.0
11	969.0
12	1010.0
13	1018.0
14	1026.0
15	1146.5
16	1267.0
17	1323.5
18	1380.0
19	1344.0
20	1308.0
21	1725.0
22	2142.0
23	2200.0
24	2258.0
25	3045.5
26	4396.0
27	4959.0
28	5546.5
29	6134.0
30	8057.5
31	9981.0
32	10648.5
33	11316.0
34	12532.0
35	13748.0
36	14383.5
37	15019.0
38	16258.5
39	17498.0
40	20446.0
41	23394.0
42	27422.5
43	31451.0
44	38474.0
45	45497.0
46	73114.5
47	100732.0
48	107607.0
49	114482.0
50	114334.0
51	114186.0
52	90271.0
53	66356.0
54	53266.0
55	40176.0
56	33406.0
57	26636.0
58	24047.0
59	21458.0
60	20080.0
61	18702.0
62	16341.5
63	13981.0
64	10688.0
65	7395.0
66	5741.0
67	4087.0
68	3154.5
69	2222.0
70	1873.5
71	1525.0
72	1270.0
73	1015.0
74	827.5
75	497.5
76	355.0
77	282.0
78	209.0
79	189.5
80	170.0
81	129.5
82	89.0
83	79.5
84	70.0
85	51.0
86	32.0
87	21.5
88	11.0
89	9.0
90	7.0
91	7.5
92	8.0
93	14.5
94	21.0
95	18.5
96	16.0
97	8.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	732798.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.283511424413312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.031931613926744	13.97754165562462
2	13.874643829531466	6.46100856229123
3	7.0729030422286145	4.940460563624929
4	4.181025588110808	3.8939582818616953
5	2.7470592106330223	3.1980592257156895
6	1.8852380966281894	2.633697765634899
7	1.3772582738099115	2.2447186126834584
8	1.0290789871923083	1.9168457883932628
9	0.8111662695193175	1.6998119193106578
>10	6.192374707603887	31.4949686359705
>50	0.6408313807004022	9.567463852035322
>100	0.12852328526581394	5.569879620429143
>500	0.017850456286918604	2.9057470689205265
>1k	0.006545167305203488	2.5872426104744366
>5k	0.0029750760478197674	5.404462341879935
>10k+	5.950152095639534E-4	1.5041334951497165
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	10857	1.4815815545348106	TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	8565	1.1688077751303907	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCG	8507	1.1608929063671025	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC	7702	1.0510399864628452	No Hit
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT	7369	1.005597722701208	TruSeq Adapter, Index 22 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGC	6867	0.9370931689224044	No Hit
TCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	3466	0.4729816402337343	TruSeq Adapter, Index 20 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	2419	0.33010461273092995	TruSeq Adapter, Index 22 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	1791	0.24440568888015524	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCT	1779	0.24276812982568183	No Hit
AGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	1673	0.22830302484450013	TruSeq Adapter, Index 22 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	1408	0.19214026239154583	No Hit
CGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	1381	0.18845575451898067	TruSeq Adapter, Index 22 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	1272	0.17358125977418062	TruSeq Adapter, Index 22 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTC	1252	0.1708519946833916	No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCT	1151	0.15706920597490714	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1083	0.14778970466622451	No Hit
AAGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC	994	0.13564447501221347	No Hit
TGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	965	0.1316870406305694	TruSeq Adapter, Index 22 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTA	956	0.13045887133971437	No Hit
GGCCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCT	909	0.1240450983763602	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTGTTTT	814	0.11108108919511243	No Hit
GGGCCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC	801	0.10930706688609956	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCC	782	0.10671426504985002	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	780	0.10644133854077113	No Hit
AGGCCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC	768	0.10480377948629772	No Hit
GGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCT	760	0.10371207344998214	No Hit
AGCCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCT	748	0.10207451439550871	No Hit
GTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	738	0.10070988185011422	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3646325453945017E-4	0.0	0.0	1.0600465612624488	0.0
2	1.3646325453945017E-4	0.0	0.0	4.7034789942112285	0.0
3	1.3646325453945017E-4	0.0	0.0	6.293275909595823	0.0
4	1.3646325453945017E-4	0.0	0.0	8.040278494209865	0.0
5	1.3646325453945017E-4	0.0	0.0	13.393868433047034	0.0
6	1.3646325453945017E-4	0.0	0.0	15.29084413439993	0.0
7	1.3646325453945017E-4	0.0	0.0	17.483126318576197	0.0
8	1.3646325453945017E-4	0.0	0.0	20.519570195333504	0.0
9	1.3646325453945017E-4	0.0	0.0	21.48600296398189	0.0
10	1.3646325453945017E-4	0.0	0.0	24.86810826448762	0.0
11	1.3646325453945017E-4	0.0	0.0	28.392544739478	0.0
12	1.3646325453945017E-4	0.0	0.0	31.73016301900387	0.0
13	1.3646325453945017E-4	0.0	0.0	32.791437749557176	0.0
14	1.3646325453945017E-4	0.0	0.0	33.279976200808406	0.0
15	1.3646325453945017E-4	0.0	0.0	34.25732602981995	0.0
16	1.3646325453945017E-4	0.0	0.0	35.85080745307711	0.0
17	1.3646325453945017E-4	0.0	0.0	37.60313210461819	0.0
18	1.3646325453945017E-4	0.0	0.0	39.21189741238377	0.0
19	1.3646325453945017E-4	0.0	0.0	40.545279872488734	0.0
20	1.3646325453945017E-4	0.0	0.0	41.801014740760756	0.0
21	5.458530181578007E-4	0.0	0.0	43.256941203442146	0.0
22	8.18779527236701E-4	0.0	0.0	44.757081760594325	0.0
23	0.0010917060363156014	0.0	0.0	46.075589725954494	0.0
24	0.001637559054473402	0.0	0.0	47.0850084197828	0.0
25	0.0019104855635523022	0.0	0.0	47.96492348505318	0.0
26	0.0019104855635523022	0.0	0.0	48.74644854380061	0.0
27	0.0019104855635523022	0.0	0.0	49.47147781516871	0.0
28	0.0019104855635523022	0.0	0.0	50.271698339788045	0.0
29	0.0019104855635523022	0.0	0.0	51.06454984866225	0.0
30	0.0020469488180917526	0.0	0.0	51.92863517640605	0.0
31	0.0020469488180917526	0.0	0.0	52.81373584534892	0.0
32	0.002319875327170653	0.0	0.0	53.57001520200656	0.0
33	0.002319875327170653	0.0	0.0	54.28235339070248	0.0
34	0.002456338581710103	0.0	0.0	54.952797360254806	0.0
35	0.002456338581710103	0.0	0.0	55.61136902666219	0.0
36	0.002592801836249553	0.0	0.0	56.35454791088404	0.0
37	0.002592801836249553	0.0	0.0	57.058152451289445	0.0
38	0.0027292650907890033	0.0	0.0	57.764213330276554	0.0
39	0.0027292650907890033	0.0	0.0	58.442572168592164	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	20	7.0316094E-4	45.000004	1
CGGGTAT	40	6.8084773E-9	45.000004	6
TCGTTCG	20	7.0316094E-4	45.000004	45
TCGTTAG	20	7.0316094E-4	45.000004	1
TCGCACA	20	7.0316094E-4	45.000004	16
TCGCAAA	20	7.0316094E-4	45.000004	10
TTAATCG	45	3.8380676E-10	45.000004	20
GATCGGC	20	7.0316094E-4	45.000004	10
GGTAACT	45	3.8380676E-10	45.000004	8
GATCGAT	20	7.0316094E-4	45.000004	12
ACGTTAG	20	7.0316094E-4	45.000004	1
TAGACCG	20	7.0316094E-4	45.000004	2
AACCCGA	20	7.0316094E-4	45.000004	36
CCCTACA	20	7.0316094E-4	45.000004	27
TTTCGCG	20	7.0316094E-4	45.000004	1
GACAATT	20	7.0316094E-4	45.000004	35
CGCATAG	20	7.0316094E-4	45.000004	27
CGTTGAT	45	3.8380676E-10	45.000004	25
CTCCCGT	20	7.0316094E-4	45.000004	13
GAATCGT	20	7.0316094E-4	45.000004	18
>>END_MODULE