FastQCFastQC Report
Sat 18 Jun 2016
SRR3552533_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552533_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488726
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC26040.5328138875361654No Hit
CTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGCT20420.41782102855178566No Hit
CCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC18060.3695322123234696No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA14550.29771282886525374No Hit
TCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC10840.22180117284531617No Hit
TGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGCTT9520.19479217393795295Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG9160.1874260833268539No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8210.1679877886586758No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCC8070.16512319786547064No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGC7000.14322953966025953No Hit
ACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC6640.13586344904916045No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCG6260.12808813118188922No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTC6220.1272696766695449No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5660.11581131349672413No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGG302.1629312E-645.0000042
AGCGTAA207.029303E-445.020
CAACGCT207.029303E-445.042
TTATGCG406.8012014E-945.01
ATAGCGG850.045.02
CGCTACA207.029303E-445.028
CGTAAGC207.029303E-445.022
ATGCGAG253.887504E-545.01
TATAACG207.029303E-445.01
AACGGGC700.041.7857134
TCGGCGT1850.041.3513534
CGTTCAG1350.040.038
CATAGCG451.9244908E-840.01
TAATAGG403.4527875E-739.3752
GGTCGGC2000.039.3752
CGTTAGG700.038.5714262
TAGGACG356.2409217E-638.5714261
GTCTCGC659.094947E-1238.0769271
GTGGCGT1600.037.9687534
ACACGCG1250.037.80000336