##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552533_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	488726
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60665280750359	34.0	31.0	34.0	31.0	34.0
2	32.75605963259577	34.0	31.0	34.0	31.0	34.0
3	32.79730564774536	34.0	31.0	34.0	31.0	34.0
4	36.186409153595264	37.0	37.0	37.0	35.0	37.0
5	36.217193273940815	37.0	37.0	37.0	35.0	37.0
6	36.253465131791636	37.0	37.0	37.0	35.0	37.0
7	36.295556201225224	37.0	37.0	37.0	35.0	37.0
8	36.28439452781313	37.0	37.0	37.0	35.0	37.0
9	37.783571981028224	39.0	38.0	39.0	35.0	39.0
10	37.87294312150367	39.0	38.0	39.0	35.0	39.0
11	37.812289503730106	39.0	38.0	39.0	35.0	39.0
12	37.653490913108776	39.0	37.0	39.0	35.0	39.0
13	37.59253037489309	39.0	37.0	39.0	35.0	39.0
14	38.90153582989242	40.0	38.0	41.0	35.0	41.0
15	38.97571236234618	40.0	38.0	41.0	35.0	41.0
16	39.022806234986476	40.0	38.0	41.0	35.0	41.0
17	39.02693738413753	40.0	38.0	41.0	35.0	41.0
18	39.02564627214431	40.0	38.0	41.0	35.0	41.0
19	39.00379967507356	40.0	38.0	41.0	35.0	41.0
20	38.955322614307406	40.0	38.0	41.0	35.0	41.0
21	38.9033446143647	40.0	38.0	41.0	35.0	41.0
22	38.88996697536043	40.0	38.0	41.0	35.0	41.0
23	38.84920998678196	40.0	38.0	41.0	35.0	41.0
24	38.82752708061368	40.0	38.0	41.0	35.0	41.0
25	38.78072171318899	40.0	38.0	41.0	35.0	41.0
26	38.75426721721374	40.0	38.0	41.0	35.0	41.0
27	38.72347491232306	40.0	38.0	41.0	35.0	41.0
28	38.70537274464628	40.0	38.0	41.0	35.0	41.0
29	38.6403383490954	40.0	38.0	41.0	35.0	41.0
30	38.556207363635245	40.0	38.0	41.0	35.0	41.0
31	38.47499826078416	40.0	37.0	41.0	35.0	41.0
32	38.37768401926642	40.0	37.0	41.0	35.0	41.0
33	38.30271972434452	40.0	37.0	41.0	35.0	41.0
34	38.16076288145096	40.0	37.0	41.0	34.0	41.0
35	38.10866006719512	40.0	37.0	41.0	34.0	41.0
36	38.075385798995754	40.0	37.0	41.0	34.0	41.0
37	38.00579261181112	40.0	36.0	41.0	34.0	41.0
38	37.96363197374398	40.0	36.0	41.0	34.0	41.0
39	37.90864001505956	40.0	36.0	41.0	34.0	41.0
40	37.78488560052054	40.0	36.0	41.0	34.0	41.0
41	37.719335578626875	40.0	36.0	41.0	34.0	41.0
42	37.70366217471548	40.0	35.0	41.0	34.0	41.0
43	37.67682095898315	40.0	35.0	41.0	34.0	41.0
44	37.62420865679338	40.0	35.0	41.0	34.0	41.0
45	37.58059526196683	40.0	35.0	41.0	34.0	41.0
46	37.48060058192116	39.0	35.0	41.0	33.0	41.0
47	37.392645367752074	39.0	35.0	41.0	33.0	41.0
48	37.34871891407455	39.0	35.0	41.0	33.0	41.0
49	37.30622680192992	39.0	35.0	41.0	33.0	41.0
50	37.21622749761625	39.0	35.0	41.0	33.0	41.0
51	36.62380147567349	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	7.0
14	8.0
15	6.0
16	23.0
17	21.0
18	58.0
19	95.0
20	177.0
21	283.0
22	471.0
23	692.0
24	1171.0
25	1621.0
26	1972.0
27	2463.0
28	2811.0
29	3205.0
30	3840.0
31	5031.0
32	6602.0
33	9898.0
34	22873.0
35	40942.0
36	26876.0
37	43303.0
38	86531.0
39	227058.0
40	685.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.83371868899956	20.535228328347582	25.78929707034207	12.841755912310784
2	29.632759460311092	26.00966594779079	27.08388749524273	17.273687096655387
3	31.778747191677954	24.720395477220364	27.101893494514307	16.39896383658737
4	29.44226417256295	25.396234290788705	25.99575222108093	19.165749315567414
5	26.16189848708683	28.6577755224809	24.9374905366197	20.242835453812567
6	26.719061396365245	33.84084333552952	23.198683925144152	16.241411342961086
7	77.65557797211525	6.957272582183063	9.546862659240556	5.840286786461125
8	78.1933025867255	6.491571964659134	8.884323731497814	6.430801717117567
9	72.44713806918396	7.80989757041778	11.249248044916783	8.493716315481477
10	42.69795345449188	22.38166170819641	18.424024913755353	16.496359923556348
11	31.86898180166392	25.478898196535482	22.789865896228154	19.862254105572447
12	27.238575398075817	22.73196023947979	29.1842463875464	20.845217974898
13	24.283954608512744	25.486264287146582	30.070632624415317	20.159148479925356
14	20.026968076181745	28.38584401075449	28.838040128824737	22.74914778423902
15	19.139967998428567	26.016622811145712	33.38250880861669	21.46090038180903
16	21.006862741086007	25.229678797526628	30.597921943993157	23.165536517394205
17	21.35429668157618	24.908026174175305	28.091404999938618	25.6462721443099
18	21.326469228156473	25.31602574857896	29.810364089489816	23.547140933774756
19	23.30692453440169	26.379812000998516	27.89824973502535	22.415013729574447
20	24.147886545835497	27.314896281351924	27.75522480899318	20.7819923638194
21	23.677070587609418	27.500890069282175	27.657624108396117	21.16441523471229
22	21.64648494248311	26.852264868249286	26.39679493212966	25.104455257137946
23	21.397470157102344	27.106190380704113	27.339654530350337	24.156684931843202
24	22.26114428125371	25.290039817812026	28.32855219489039	24.120263706043875
25	20.608275393574313	27.775686171801787	26.759574894726292	24.85646353989761
26	19.48105891644807	27.968636823086968	28.718750383650555	23.83155387681441
27	21.701116781182094	27.72535121929261	27.61506447375421	22.958467525771088
28	19.12298506729742	27.088388995060626	28.71077045215519	25.077855485486754
29	22.169068148614972	25.387435904781004	28.876507490904924	23.566988455699104
30	23.57537761445063	27.134836288636166	26.594451696860816	22.69533440005238
31	22.891763483015023	27.655577972115253	25.125939688086984	24.326718856782737
32	23.974578802846587	28.58043157106436	25.278172227383035	22.166817398706023
33	25.050232645695136	27.061993837037523	24.133358978241386	23.754414539025955
34	21.70439059923147	25.28103681817624	28.761514631920544	24.253057950671746
35	22.891968096643108	26.736862782008735	26.70964916947328	23.661519951874872
36	24.141338909736746	28.163224383396834	26.287940481987864	21.40749622487856
37	22.895855755576743	27.93016946100678	27.728625037341985	21.445349746074488
38	24.01140925590208	28.39055012420047	24.55486305209872	23.043177567798725
39	27.226912421274907	24.12803902391115	26.933905705855633	21.71114284895831
40	24.35495553745862	25.097293780154935	29.0841903234123	21.46356035897415
41	21.486067858063617	27.0196388160237	27.161640673915443	24.332652651997233
42	22.62474269836268	26.345846138736224	27.42600148140267	23.603409681498427
43	24.38032762734129	24.568572165180488	26.76632714445313	24.28477306302509
44	22.98813650184357	24.316488175378435	27.400834005148077	25.29454131762992
45	23.80802330958451	23.38099466776885	26.994266726141024	25.81671529650561
46	23.741114653200363	25.783363275127574	27.479405638333137	22.996116433338926
47	21.246669913202897	25.296792067538863	30.578892876581154	22.877645142677082
48	21.883018296550624	24.701161796180273	28.84172317413029	24.574096733138813
49	23.70776263182233	22.9905918653806	29.973850378330596	23.32779512446647
50	21.82224804900906	22.956830616746398	29.06495664237221	26.155964691872335
51	21.812631208489012	22.44325041025032	27.24696455682734	28.497153824433326
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	192.0
1	367.0
2	542.0
3	931.0
4	1320.0
5	996.0
6	672.0
7	640.5
8	609.0
9	634.5
10	660.0
11	689.0
12	718.0
13	790.0
14	862.0
15	870.5
16	879.0
17	991.0
18	1103.0
19	1157.5
20	1212.0
21	1320.5
22	1429.0
23	1775.0
24	2121.0
25	3257.5
26	4858.5
27	5323.0
28	5841.5
29	6360.0
30	7068.0
31	7776.0
32	8696.5
33	9617.0
34	10505.5
35	11394.0
36	12084.0
37	12774.0
38	13798.0
39	14822.0
40	17200.0
41	19578.0
42	21621.0
43	23664.0
44	27132.0
45	30600.0
46	37652.5
47	44705.0
48	49062.5
49	53420.0
50	52529.5
51	51639.0
52	44524.0
53	37409.0
54	33055.0
55	28701.0
56	25933.0
57	23165.0
58	21951.5
59	20738.0
60	20207.5
61	19677.0
62	17702.0
63	15727.0
64	13923.5
65	12120.0
66	9824.0
67	7528.0
68	6491.5
69	5455.0
70	4765.0
71	4075.0
72	3310.0
73	2545.0
74	2088.0
75	1281.5
76	932.0
77	703.5
78	475.0
79	293.0
80	111.0
81	76.0
82	41.0
83	22.5
84	4.0
85	4.5
86	5.0
87	3.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	488726.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.979144981973562
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.26656200938088	22.150854289008528
2	11.510950095732243	7.362206839833677
3	4.1380407596568425	3.969930161831465
4	2.054526127570399	2.6280795561130605
5	1.3834581067965719	2.212090368686712
6	1.0549702553112332	2.0242228047760555
7	0.9276134542172733	2.076499959775043
8	0.8183868719762757	2.0937049944218526
9	0.7518709650320166	2.163977153864571
>10	7.94752590790744	45.23340201072882
>50	0.08265926573696573	1.7325361005783289
>100	0.05446540765614022	3.142539756176139
>500	0.005766925516532493	1.3675867457433197
>1k	0.0032038475091847184	1.8423692584624194
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	2604	0.5328138875361654	No Hit
CTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGCT	2042	0.41782102855178566	No Hit
CCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	1806	0.3695322123234696	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1455	0.29771282886525374	No Hit
TCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	1084	0.22180117284531617	No Hit
TGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGCTT	952	0.19479217393795295	Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	916	0.1874260833268539	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	821	0.1679877886586758	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCC	807	0.16512319786547064	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGC	700	0.14322953966025953	No Hit
ACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	664	0.13586344904916045	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCG	626	0.12808813118188922	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTC	622	0.1272696766695449	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	566	0.11581131349672413	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4391008458727385	0.0
2	2.0461362808608504E-4	0.0	0.0	1.7633602468458809	0.0
3	2.0461362808608504E-4	0.0	0.0	2.336687632743091	0.0
4	2.0461362808608504E-4	0.0	0.0	3.014572582592291	0.0
5	2.0461362808608504E-4	0.0	0.0	4.861824416953467	0.0
6	2.0461362808608504E-4	0.0	0.0	5.793430265629412	0.0
7	2.0461362808608504E-4	0.0	0.0	6.879724017138438	0.0
8	2.0461362808608504E-4	0.0	0.0	8.484508702217603	0.0
9	2.0461362808608504E-4	0.0	0.0	9.142750743770538	0.0
10	2.0461362808608504E-4	0.0	0.0	10.759607632906782	0.0
11	4.092272561721701E-4	0.0	0.0	12.973936316054395	0.0
12	4.092272561721701E-4	0.0	0.0	14.785994606384763	0.0
13	4.092272561721701E-4	0.0	0.0	15.467357987911427	0.0
14	4.092272561721701E-4	0.0	0.0	15.743381772199555	0.0
15	4.092272561721701E-4	0.0	0.0	16.241411342961086	0.0
16	4.092272561721701E-4	0.0	0.0	17.224579825914724	0.0
17	4.092272561721701E-4	0.0	0.0	18.415226527747652	0.0
18	4.092272561721701E-4	0.0	0.0	19.777339449916724	0.0
19	4.092272561721701E-4	0.0	0.0	20.62198450665608	0.0
20	4.092272561721701E-4	0.0	0.0	21.36943809005455	0.0
21	4.092272561721701E-4	0.0	0.0	22.224518441826298	0.0
22	4.092272561721701E-4	0.0	0.0	23.09105715677087	0.0
23	4.092272561721701E-4	0.0	0.0	23.900713283107507	0.0
24	8.184545123443402E-4	0.0	0.0	24.605811845492155	0.0
25	8.184545123443402E-4	0.0	0.0	25.201851344106924	0.0
26	8.184545123443402E-4	0.0	0.0	25.724434550238783	0.0
27	8.184545123443402E-4	0.0	0.0	26.247631597254905	0.0
28	8.184545123443402E-4	0.0	0.0	26.774307075948485	0.0
29	8.184545123443402E-4	0.0	0.0	27.307120963484653	0.0
30	0.0010230681404304251	0.0	0.0	27.86694384992818	0.0
31	0.0010230681404304251	0.0	0.0	28.436383576891757	0.0
32	0.0010230681404304251	0.0	0.0	28.979632759460312	0.0
33	0.0010230681404304251	0.0	0.0	29.489939147907005	0.0
34	0.0010230681404304251	0.0	0.0	29.998199400072842	0.0
35	0.0010230681404304251	0.0	0.0	30.53183174212135	0.0
36	0.0010230681404304251	0.0	0.0	31.047458084898288	0.0
37	0.0010230681404304251	0.0	0.0	31.557150632460726	0.0
38	0.0010230681404304251	0.0	0.0	32.06541088462656	0.0
39	0.0012276817685165102	0.0	0.0	32.56835118246216	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTACGG	30	2.1629312E-6	45.000004	2
AGCGTAA	20	7.029303E-4	45.0	20
CAACGCT	20	7.029303E-4	45.0	42
TTATGCG	40	6.8012014E-9	45.0	1
ATAGCGG	85	0.0	45.0	2
CGCTACA	20	7.029303E-4	45.0	28
CGTAAGC	20	7.029303E-4	45.0	22
ATGCGAG	25	3.887504E-5	45.0	1
TATAACG	20	7.029303E-4	45.0	1
AACGGGC	70	0.0	41.785713	4
TCGGCGT	185	0.0	41.351353	4
CGTTCAG	135	0.0	40.0	38
CATAGCG	45	1.9244908E-8	40.0	1
TAATAGG	40	3.4527875E-7	39.375	2
GGTCGGC	200	0.0	39.375	2
CGTTAGG	70	0.0	38.571426	2
TAGGACG	35	6.2409217E-6	38.571426	1
GTCTCGC	65	9.094947E-12	38.076927	1
GTGGCGT	160	0.0	37.96875	34
ACACGCG	125	0.0	37.800003	36
>>END_MODULE